LOCUS       NZ_CP060918             3374 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC18 plasmid pEC18-5, complete sequence.
ACCESSION   NZ_CP060918
VERSION     NZ_CP060918.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904660
            Assembly: GCF_022558825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558825.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:22:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,177
            CDSs (total)                      :: 5,051
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 74 of 262
            Pseudo Genes (incomplete)         :: 190 of 262
            Pseudo Genes (internal stop)      :: 44 of 262
            Pseudo Genes (multiple problems)  :: 42 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060918.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC18"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC18-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2016-12-20"
                     /collected_by="Long Sun"
     gene            join(3201..3374,1..345)
                     /locus_tag="H9203_RS25760"
                     /old_locus_tag="H9203_25490"
     CDS             join(3201..3374,1..345)
                     /locus_tag="H9203_RS25760"
                     /old_locus_tag="H9203_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_052969336.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTALSVTGGIFLGCWGL
                     MEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGVKGENTWTVGD
                     KNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            352..585
                     /locus_tag="H9203_RS25765"
                     /old_locus_tag="H9203_25495"
     CDS             352..585
                     /locus_tag="H9203_RS25765"
                     /old_locus_tag="H9203_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1903..2094)
                     /locus_tag="H9203_RS25770"
                     /old_locus_tag="H9203_25500"
     CDS             complement(1903..2094)
                     /locus_tag="H9203_RS25770"
                     /old_locus_tag="H9203_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2158..2481
                     /locus_tag="H9203_RS25775"
                     /old_locus_tag="H9203_25505"
     CDS             2158..2481
                     /locus_tag="H9203_RS25775"
                     /old_locus_tag="H9203_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2471..>3313
                     /locus_tag="H9203_RS25780"
                     /pseudo=""
     CDS             2471..>3313
                     /locus_tag="H9203_RS25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          1739..1822
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.882"
                     /coverage="95.0"
                     /evalue="4.11e-16"
     Typing          19..668
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.036"
                     /coverage="98.0"
                     /evalue="6.800000000000001e-100"
     Typing          2472..3352
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.619"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cagttcagca ccacagaggc cgaactgtcc cggcagcaga aaaaactgct ctggcgggtg
       61 atcaaagggc ggatactgtt tccggcgctg accgcgctca gcgtcacggg cgggattttc
      121 ctggggtgct gggggctgat ggagtggcag gaaagcaaga ttgcgaaaaa tatcctgact
      181 atcagagagc aggaaaacac gctggcgaaa ctcgaggcaa aaacctgggg ggtgacgttc
      241 gtcaatggcg agaacgggaa atttctggtg ttgccggacg gggtgaaagg ggaaaacacc
      301 tggacggtgg gcgacaagaa cgccgtgagg ctggtcaggg agtaaacgag tatgacggaa
      361 ctggaagggc atttgctgaa cgccttagag catttgcaac aggactatat gcggcggctg
      421 aacgagtggg agagcgcctt cgcggaattg cagaagatgc acgaggttac gcaacggaac
      481 aacgcgatac tgaacgagcg cgtcgtgata ttgagtcagc aggtgcagag attagccggg
      541 caagtggatc gcttgagtcg gttatttatc gcgaacaaca gctaagggat gaacaggagc
      601 gtgcccggta cagacatgag gtacaggaac gcgcggcacg ggttcggagt gggccatcaa
      661 tggagatgtg accggagaag tgacgaaatc ttgccatttt gacccgttcg acggctgggg
      721 cgagcgaaac aaatcgtgtg ccgtatttgt gcatagctat gcaacatcct gaaaacgcat
      781 ttcacgcgtt tttggggatt cagccagggg aaaacatggt ttttttactt tttgagcgta
      841 aagacgtgaa aaatctcgtt attgcacttg cgatctttcg tcggtgggcg tagaacgaaa
      901 acagctcgcc aggaaggcga gctgtttcgg gttcattgag gtctctgacc tcctcaatgt
      961 tcctgtggaa actgttgaga tccacaacag aaaccagttc attcagtttg catgagcctt
     1021 ctgtattctt caagagtttt tcaacagcac agtctcccag gaaccccgaa aaaaacctgt
     1081 ttttgcagat acttgcgtat ttgcgagaat cgtttgcggt tgggggagct gtgacgacgg
     1141 cgaccgcacg cgtgagagtt ccgctaccgg cgcagagagt ttcatcgaca gcggacacct
     1201 gatgagttgc cacaaaaacc gtatgtagcg cggctagatt ggttgagtta gaccttggcg
     1261 gcagttcccg gtatgtggtg gcgtgacggg tgagatggtg cggcgaagac gcatacgtca
     1321 cgcaggcgcg ggttcttcca ctgacgcgcc ccgtgatcga tggccttggc tccgtccggc
     1381 atatcgagag cggcaaacta cccgtccctt cactgggagg ggtgagttgt tgccgttccc
     1441 tccgaccttc caccatccag catatcgcac gatcccatgt gcaccgctac tcctgataac
     1501 tccagtgcaa aaaaaaaaaa cggatctgca tcacaacgag agtgatccca ccgctcgccg
     1561 cagccccagc gaccgaacgt agtgagcgag tgggcgagga agcggaagag tcacagaggc
     1621 aagcattgtg cacttacgcg cctgtgccag tgtccgcgtg tgtgttgttt atcgggcgcg
     1681 ctgtttttac gcatctgtgc ggctttttac tcctgacatg gtgcacttcc catccactct
     1741 gcaccgatgc aaaatgagga aagccagtat acacatcgct atcgctctgt gaactgggtc
     1801 agggctgcgc cccgacaccc gcactgacgc gccctggcgg gcttgtctgc tcccggcatc
     1861 cgctcacagg caagctgtga acgtctccgg gagctgcatg tttcagttgc tttcaccgtc
     1921 atcgccgaag cgtgcgaggc aactgcggta gagttcgtcg gcgtggtcgt gaagtgattc
     1981 gcagatgtct gcctgttcgt ccgcgtccag atcgttgagt ttttccagca gggttagcgt
     2041 ctggtttctg ataaagcgag ccatgttaag ggctgttttt tcctgtttgg tcatggtctt
     2101 aaggctcctt gtatggggga tttgttaggg ggaatgatac cgatgaaacg cgagaagatg
     2161 ctcacgatac gggttaccga tgaggagcac gcccggttac tggaacgctg cgagggtaaa
     2221 cgactggcgg aatggatgcg gcgggtctgt ctgggggagc cggttgcacg cacaggaaaa
     2281 ctgccgacac tggcaccgcc attgctgcgg catctggctg ccatcgggaa caacctgaac
     2341 cagacagccc ggaaggtgaa cagcggccag tggtcttcga ttgaccgggt tcatgtggtg
     2401 gccgcactga tggccatcga gggtgaattg cgccagttgc ggcaggctgt cagggaacag
     2461 ggggtacggg atgatagtta aatttcatgc cagggggaaa ggcggtggaa gtggcccggt
     2521 cgattacctg ctggggcggg agcgtaaccg cgaaggcgca acggtgctcc ggggtaaccc
     2581 ggaagaagtg cgggaactca ttgattccac gccattttcg aagaaataca cgtcaggcgt
     2641 tctgtcgttc gcggagaagg agctaccacc gggtgaacgc gaaagagtga tgacgagctt
     2701 tgagcgtgtt cttatgcccg gtctcgaaaa agaccagtac agcattctct gggtggaaca
     2761 ccaggataag gggcggctcg aactgaattt tgtcatcccg aacatggagc tacagagcgg
     2821 taagcgcctc cagccgtact acgaccgtgc ggacaggccg aggataaatg cctggcagac
     2881 gctggttaat catcattacg ggctgcgtga cccgaacgca ccggagaaca gacggacgct
     2941 tgtcacgcca aataatctgc caaaagcgaa acaggaggcc gcagaagcga ttacacgggg
     3001 tcttgaggct ctttaccatg ccggagcaat aaaaacgcgt caggatgtca cagaggcgct
     3061 tacagcggca ggttttgagg tggtgaggac aaccaaaagc agcatcagta ttgccgaccc
     3121 ggaggggggg cggaatctca ggctaaaggg ggcgctgtat gagcaatctt ttacaaatgg
     3181 gaacagactt cgagaagaaa ctgaaagagc gagccgcttc taccgagaac atgctgaaca
     3241 gcgagttcag gaagctcgaa gagtctgtag acaaggcatt gagcttaaac aggcagaaaa
     3301 tcagagacgc tatcagcgaa catacaacat ccgtgaaaaa gcaactggat acgctgagca
     3361 ccaccgtgag catg
//
