LOCUS       NZ_CP060912             4091 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC19 plasmid pEC19-5, complete sequence.
ACCESSION   NZ_CP060912
VERSION     NZ_CP060912.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904661
            Assembly: GCF_022558785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558785.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:22:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,991
            CDSs (total)                      :: 4,870
            Genes (coding)                    :: 4,617
            CDSs (with protein)               :: 4,617
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 253
            CDSs (without protein)            :: 253
            Pseudo Genes (ambiguous residues) :: 0 of 253
            Pseudo Genes (frameshifted)       :: 85 of 253
            Pseudo Genes (incomplete)         :: 163 of 253
            Pseudo Genes (internal stop)      :: 44 of 253
            Pseudo Genes (multiple problems)  :: 33 of 253
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060912.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC19"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC19-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-05-28"
                     /collected_by="Long Sun"
     gene            complement(join(3760..4091,1..1816))
                     /gene="mobQ"
                     /locus_tag="H9204_RS24825"
                     /old_locus_tag="H9204_24635"
     CDS             complement(join(3760..4091,1..1816))
                     /gene="mobQ"
                     /locus_tag="H9204_RS24825"
                     /old_locus_tag="H9204_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_011666798.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     KRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            1987..2277
                     /locus_tag="H9204_RS24830"
                     /old_locus_tag="H9204_24640"
     CDS             1987..2277
                     /locus_tag="H9204_RS24830"
                     /old_locus_tag="H9204_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            2810..3511
                     /locus_tag="H9204_RS24835"
                     /old_locus_tag="H9204_24645"
     CDS             2810..3511
                     /locus_tag="H9204_RS24835"
                     /old_locus_tag="H9204_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          1462..1816
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1833..1983
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          2811..3510
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..1858)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.838"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cattaagccg gacaacaccg cccatagagt catccacacc catgaccagc aaaagctgaa
       61 gctggcgggt ttcacggtcg acagaactca ttaccggcat cagagtcggt ggcagacgtc
      121 cctgcgtaaa atccaccttc cttgccgcca gtgatggcat ggctggctgg cggtcttctc
      181 caagacagaa agactccggc catgtcttcg gctggcggag atcgcgggta tgttgcggcc
      241 atgtctggca aacctgcgtc agcagttgct caaaggtcgc agaggaataa acatgttgca
      301 gtggtggacg ttgaccgatt tcgcttcgct cacagatata accactcctg tcacgcatta
      361 aatcaacact ctcaaaaatt ctggcgcggt atttctccag ttcgttcccc cagagcgtat
      421 attctccgtt ccggttgtga agcagaaggt aacaggtcat ttccccgtta cctggcatgg
      481 gggcctcgcc ataccccgta acgactgctt tcagtacccc tgaatccata acggcattgt
      541 catttctcct gagctgctcc gcccgacgac gttctgtttc ctcctgctcc ttccggcgca
      601 attcctcttc acgtttacgc aactgttctc ggagatctgc aaggccagat gcaccccact
      661 gctgccactg tgtttcaaat gctttttgac tcgcgggaat gtcctgtttc tcaaccttcc
      721 agtgatgctg ccgcaatttt tccatgcgct ggctcaacga ctggattttt tcctgttcgc
      781 ggccgtattc cggtgcaacc cattgcatca ggggaatacg ggaaaaaccg gacgaaaacg
      841 gcgatgattt tttcaggctg gcagcatggc tcatcgcttc ctttcgttcg gtgttcagat
      901 cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat cgacatggct ttactggtga
      961 gtttccatgc tgcgttactg tccgtcggca acgcttcgat gatgtcatgc agtatgtacg
     1021 aagttgcgtt gtcaggaagc tcaatattca gaaggtcagc catacggcgt gcgggctctg
     1081 tcagctcact gttcctgagt tcgtcgcggg aaacctgtat ttccaggtta agcctgttaa
     1141 tcaggcgatt ctgttcgccg cgttctgttt cgatgcccca cttttccatc gcacaggccg
     1201 actttccgag gtgaatgcca ggcgttttct caaccccctg tcgctcgtat gaacgatggt
     1261 ctatctcttc ctgataaccg gcgttcgcca gcgccctgtt cgcatggtct gcccatgatc
     1321 cccgccatgt ttcggcgtga gtccagtcat tccactcacg gactttctcc ccgaagccgt
     1381 caggcgtgat tgccctcatg gatagcatga tgtgcgcgtg tgggtttgac ccgcccagat
     1441 tgtgaaaggc cacgtcggcg atcatgccgc gactgacaaa gttttccctt acgaagttga
     1501 gaactgtttc ccgtgcggca tcctgcgcca gttcacgggg gatggcgagt tcgaactccc
     1561 gcgcaagttg tgagtttttc cgccgctcgg ctttttcaac agcattccag agttcggcgc
     1621 ggttcgcaca ccatgctggg gcgtttgctg gcgtcagaat cactgcgctg gctacaccgt
     1681 ttttgcgggt gtagtcgtga atcactccgg tgcgctcatc cagtattttt tctccggcac
     1741 gataggcagc agaagcaacg gcgctgtagc ccttgcttct ggaaagaatc ttcatgctga
     1801 gatgataaat tgccatcgtg tcgttgttgc cgttttcttt tagccccgca gggcgcacac
     1861 aaactcgacg aaggagagtt tgtataagtg cgcacttcgt gatttgctgt gcattatgcc
     1921 agttcagttt ttaaactacc agtgacgcgc taaaatgcga tttttcaccg cgcacggagt
     1981 tacggaatga gtagaacact cgaacagaag attgccgatg ctgaggccag attgcagcgt
     2041 ctgaaggcga agagcaggag cctggacaca gcgcagaagg tcattgtggg agcggcattg
     2101 ctggcaaagg tcaggaagcc tggggaaaca caactacgag cctggttgct tcatctcctg
     2161 aagacagagg taacacggca ggctgatgtg agcaggatac agccgttgat tgacgaactg
     2221 aacgcgttac caaaaccagt ccccaaagaa gtgtcagaaa acgaccaaca ggcgtgaatg
     2281 caccgtccgc tcgccgcagc cccagcgacc gagcgttagc gagcgagtgg gcgaggaagc
     2341 ggaagtgaat ccgtcgccag attgagcaca tacgcacggt ttttcgacag gtgatatgcg
     2401 ctgaacccgc atgaatactg gatttccaca gggttgagcg gggttttgct aaagcttgcg
     2461 aacctgcttt tttgtaacct gttgtttttc atgatttttt gttttgaaca ccccaagttt
     2521 tccacaggct cgccgttaag tttttagatc ttgtttttaa gatcttgttt ttaatacttg
     2581 ttttagggtc gaattttctt atgaaattca agagtttaga agactatagg taatataatt
     2641 taaggtaaag gcaatatgat ttaaggtgga taggcaatat gatttaaggt aaaggcaata
     2701 tgatttaagg taatattcac aggaaaaggg atattaaaat taaatcatat tgcctttacc
     2761 taaaggaatg tttatatttt actcattacc ttaggttgag ggatttacaa tgtcggatct
     2821 gattgcctac aaatccaatg cactggttga ggccagctat aaattgactc ttcaggaaca
     2881 gcgattcttg ttactttgca ttggtcgatt gaagtcgggt tctgatgcag aatccactga
     2941 attgcaaaag accatgactg ttaccgccga agagtatttc gactcattcc ctgatatggg
     3001 gcggaaaaat gccgaggttc agctacagga agcaattgat cgtttgtggg acaggtcgat
     3061 catccttaag gacgatgaga agcgcgagga gttccgctgg atccagtatc gggcacagta
     3121 cgctaaaggt gagggcaaag cccaaatcac tttttcagat gcggtcatgc cttatctgac
     3181 acagttgaag gggcaattta ctagagttgt aattaagaac atctccaatt tatcaagaag
     3241 ttacagcata agaatatacg aacttctcca gcaattccgc agcacagggg aacgtattat
     3301 cgccctcgat gattttcgct caatgcttga tatagagcat aaatatcaaa catataagtc
     3361 tctgaaccag caattattaa gaccttgcgt tgacgaactg aatcaaaaaa gcgacttggc
     3421 tgtaacgctt gagacgatca agaaggggcg cacggttgta gccctgcatt ttcgcttcaa
     3481 ggaagacaaa cagatcaaga tgacgatcta gaatcgattt taaggcctct aaaacgctct
     3541 ctgacgcgtt ttttttgtgt ttggcatgcg aaggtactgg tgaagtaaaa aattgcgtca
     3601 taaacgctct ggcacagtga aaaaagaacc cccaattttt cgggggcggg aaaactataa
     3661 agttgatttg tgttatgtgt gtgcttcttg cattcatccc tgtcgatgaa catcgacgaa
     3721 cataacacga tgttaaacaa aacaaaatat tggtcacatt cactgtgacc aaccgcgatc
     3781 atgtctcctc tcggtggcgt gtgttcgggc atattcatca cgtttccatt catcccaggt
     3841 gcgctcaaag cccagcgagc ggaacagatg atccggctgt ttttctggcg tgttgacgat
     3901 agcaaaaagc ggttcgctcc aggttttcag atgaaactgc ataagttccg gcgcaggacg
     3961 tgccggactg tcagcatcgt ggtttaccgc ttcaccatag cctgcaaagc gtaaaccttt
     4021 gtcggtcagt gcattgatta ccagctggct gttgtcggct gtggggacgg caaacgcggg
     4081 atacagcgta c
//
