LOCUS       NZ_CP060906             4091 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC20 plasmid pEC20-5, complete sequence.
ACCESSION   NZ_CP060906
VERSION     NZ_CP060906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904662
            Assembly: GCF_022558765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558765.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 03:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,990
            CDSs (total)                      :: 4,869
            Genes (coding)                    :: 4,617
            CDSs (with protein)               :: 4,617
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 85 of 252
            Pseudo Genes (incomplete)         :: 163 of 252
            Pseudo Genes (internal stop)      :: 44 of 252
            Pseudo Genes (multiple problems)  :: 34 of 252
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060906.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC20"
                     /isolation_source="throat swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC20-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-06-22"
                     /collected_by="Long Sun"
     gene            complement(join(3942..4091,1..141))
                     /locus_tag="H9205_RS24820"
                     /old_locus_tag="H9205_24625"
     CDS             complement(join(3942..4091,1..141))
                     /locus_tag="H9205_RS24820"
                     /old_locus_tag="H9205_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            312..2459
                     /gene="mobQ"
                     /locus_tag="H9205_RS24825"
                     /old_locus_tag="H9205_24630"
     CDS             312..2459
                     /gene="mobQ"
                     /locus_tag="H9205_RS24825"
                     /old_locus_tag="H9205_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_011666798.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     KRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2708..3409)
                     /locus_tag="H9205_RS24830"
                     /old_locus_tag="H9205_24635"
     CDS             complement(2708..3409)
                     /locus_tag="H9205_RS24830"
                     /old_locus_tag="H9205_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(146..296)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          complement(313..667)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2710..3409)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          271..2456
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.863"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttccccaggc ttcctgacct ttgccagcaa tgccgctccc acaatgacct tctgcgctgt
       61 gtccaggctc ctgctcttcg ccttcagacg ctgcaatctg gcctcagcat cggcaatctt
      121 ctgttcgagt gttctactca ttccgtaact ccgtgcgcgg tgaaaaatcg cattttagcg
      181 cgtcactggt agtttaaaaa ctgaactggc ataatgcaca gcaaatcacg aagtgcgcac
      241 ttatacaaac tctccttcgt cgagtttgtg tgcgccctgc ggggctaaaa gaaaacggca
      301 acaacgacac gatggcaatt tatcatctca gcatgaagat tctttccaga agcaagggct
      361 acagcgccgt tgcttctgct gcctatcgtg ccggagaaaa aatactggat gagcgcaccg
      421 gagtgattca cgactacacc cgcaaaaacg gtgtagccag cgcagtgatt ctgacgccag
      481 caaacgcccc agcatggtgt gcgaaccgcg ccgaactctg gaatgctgtt gaaaaagccg
      541 agcggcggaa aaactcacaa cttgcgcggg agttcgaact cgccatcccc cgtgaactgg
      601 cgcaggatgc cgcacgggaa acagttctca acttcgtaag ggaaaacttt gtcagtcgcg
      661 gcatgatcgc cgacgtggcc tttcacaatc tgggcgggtc aaacccacac gcgcacatca
      721 tgctatccat gagggcaatc acgcctgacg gcttcgggga gaaagtccgt gagtggaatg
      781 actggactca cgccgaaaca tggcggggat catgggcaga ccatgcgaac agggcgctgg
      841 cgaacgccgg ttatcaggaa gagatagacc atcgttcata cgagcgacag ggggttgaga
      901 aaacgcctgg cattcacctc ggaaagtcgg cctgtgcgat ggaaaagtgg ggcatcgaaa
      961 cagaacgcgg cgaacagaat cgcctgatta acaggcttaa cctggaaata caggtttccc
     1021 gcgacgaact caggaacagt gagctgacag agcccgcacg ccgtatggct gaccttctga
     1081 atattgagct tcctgacaac gcaacttcgt acatactgca tgacatcatc gaagcgttgc
     1141 cgacggacag taacgcagca tggaaactca ccagtaaagc catgtcgatg atggcagata
     1201 tgcaggcgac gcgcagacgc tgggaagatc tgaacaccga acgaaaggaa gcgatgagcc
     1261 atgctgccag cctgaaaaaa tcatcgccgt tttcgtccgg tttttcccgt attcccctga
     1321 tgcaatgggt tgcaccggaa tacggccgcg aacaggaaaa aatccagtcg ttgagccagc
     1381 gcatggaaaa attgcggcag catcactgga aggttgagaa acaggacatt cccgcgagtc
     1441 aaaaagcatt tgaaacacag tggcagcagt ggggtgcatc tggccttgca gatctccgag
     1501 aacagttgcg taaacgtgaa gaggaattgc gccggaagga gcaggaggaa acagaacgtc
     1561 gtcgggcgga gcagctcagg agaaatgaca atgccgttat ggattcaggg gtactgaaag
     1621 cagtcgttac ggggtatggc gaggccccca tgccaggtaa cggggaaatg acctgttacc
     1681 ttctgcttca caaccggaac ggagaatata cgctctgggg gaacgaactg gagaaatacc
     1741 gcgccagaat ttttgagagt gttgatttaa tgcgtgacag gagtggttat atctgtgagc
     1801 gaagcgaaat cggtcaacgt ccaccactgc aacatgttta ttcctctgcg acctttgagc
     1861 aactgctgac gcaggtttgc cagacatggc cgcaacatac ccgcgatctc cgccagccga
     1921 agacatggcc ggagtctttc tgtcttggag aagaccgcca gccagccatg ccatcactgg
     1981 cggcaaggaa ggtggatttt acgcagggac gtctgccacc gactctgatg ccggtaatga
     2041 gttctgtcga ccgtgaaacc cgccagcttc agcttttgct ggtcatgggt gtggatgact
     2101 ctatgggcgg tgttgtccgg cttaatggta cgctgtatcc cgcgtttgcc gtccccacag
     2161 ccgacaacag ccagctggta atcaatgcac tgaccgacaa aggtttacgc tttgcaggct
     2221 atggtgaagc ggtaaaccac gatgctgaca gtccggcacg tcctgcgccg gaacttatgc
     2281 agtttcatct gaaaacctgg agcgaaccgc tttttgctat cgtcaacacg ccagaaaaac
     2341 agccggatca tctgttccgc tcgctgggct ttgagcgcac ctgggatgaa tggaaacgtg
     2401 atgaatatgc ccgaacacac gccaccgaga ggagacatga tcgcggttgg tcacagtgaa
     2461 tgtgaccaat attttgtttt gtttaacatc gtgttatgtt cgtcgatgtt catcgacagg
     2521 gatgaatgca agaagcacac acataacaca aatcaacttt atagttttcc cgcccccgaa
     2581 aaattggggg ttcttttttc actgtgccag agcgtttatg acgcaatttt ttacttcacc
     2641 agtaccttcg catgccaaac acaaaaaaaa cgcgtcagag agcgttttag aggccttaaa
     2701 atcgattcta gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta
     2761 caaccgtgcg ccccttcttg atcgtctcaa gcgttacagc caagtcgctt ttttgattca
     2821 gttcgtcaac gcaaggtctt aataattgct ggttcagaga cttatatgtt tgatatttat
     2881 gctctatatc aagcattgag cgaaaatcat cgagggcgat aatacgttcc cctgtgctgc
     2941 ggaattgctg gagaagttcg tatattctta tgctgtaact tcttgataaa ttggagatgt
     3001 tcttaattac aactctagta aattgcccct tcaactgtgt cagataaggc atgaccgcat
     3061 ctgaaaaagt gatttgggct ttgccctcac ctttagcgta ctgtgcccga tactggatcc
     3121 agcggaactc ctcgcgcttc tcatcgtcct taaggatgat cgacctgtcc cacaaacgat
     3181 caattgcttc ctgtagctga acctcggcat ttttccgccc catatcaggg aatgagtcga
     3241 aatactcttc ggcggtaaca gtcatggtct tttgcaattc agtggattct gcatcagaac
     3301 ccgacttcaa tcgaccaatg caaagtaaca agaatcgctg ttcctgaaga gtcaatttat
     3361 agctggcctc aaccagtgca ttggatttgt aggcaatcag atccgacatt gtaaatccct
     3421 caacctaagg taatgagtaa aatataaaca ttcctttagg taaaggcaat atgatttaat
     3481 tttaatatcc cttttcctgt gaatattacc ttaaatcata ttgcctttac cttaaatcat
     3541 attgcctatc caccttaaat catattgcct ttaccttaaa ttatattacc tatagtcttc
     3601 taaactcttg aatttcataa gaaaattcga ccctaaaaca agtattaaaa acaagatctt
     3661 aaaaacaaga tctaaaaact taacggcgag cctgtggaaa acttggggtg ttcaaaacaa
     3721 aaaatcatga aaaacaacag gttacaaaaa agcaggttcg caagctttag caaaaccccg
     3781 ctcaaccctg tggaaatcca gtattcatgc gggttcagcg catatcacct gtcgaaaaac
     3841 cgtgcgtatg tgctcaatct ggcgacggat tcacttccgc ttcctcgccc actcgctcgc
     3901 taacgctcgg tcgctggggc tgcggcgagc ggacggtgca ttcacgcctg ttggtcgttt
     3961 tctgacactt ctttggggac tggttttggt aacgcgttca gttcgtcaat caacggctgt
     4021 atcctgctca catcagcctg ccgtgttacc tctgtcttca ggagatgaag caaccaggct
     4081 cgtagttgtg t
//
