LOCUS       NZ_CP060898             2443 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC22 plasmid pEC22-7, complete sequence.
ACCESSION   NZ_CP060898
VERSION     NZ_CP060898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904664
            Assembly: GCF_022558725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558725.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:20:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,946
            Genes (coding)                    :: 4,682
            CDSs (with protein)               :: 4,682
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 87 of 264
            Pseudo Genes (incomplete)         :: 179 of 264
            Pseudo Genes (internal stop)      :: 57 of 264
            Pseudo Genes (multiple problems)  :: 53 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060898.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC22"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC22-7"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-07-11"
                     /collected_by="Long Sun"
     gene            322..1164
                     /gene="mobV"
                     /locus_tag="H9207_RS25160"
                     /old_locus_tag="H9207_24940"
     CDS             322..1164
                     /gene="mobV"
                     /locus_tag="H9207_RS25160"
                     /old_locus_tag="H9207_24940"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_241407653.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKIWMRSRNAYRASQSLLRGFPESR"
                     /name="mobV"
     gene            1161..1289
                     /locus_tag="H9207_RS25360"
     CDS             1161..1289
                     /locus_tag="H9207_RS25360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_277629091.1"
                     /translation="MTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="hypothetical protein"
     gene            complement(1359..1589)
                     /locus_tag="H9207_RS25165"
                     /old_locus_tag="H9207_24945"
     CDS             complement(1359..1589)
                     /locus_tag="H9207_RS25165"
                     /old_locus_tag="H9207_24945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(1576..2058)
                     /locus_tag="H9207_RS25170"
                     /old_locus_tag="H9207_24950"
     CDS             complement(1576..2058)
                     /locus_tag="H9207_RS25170"
                     /old_locus_tag="H9207_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          314..1092
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.615"
                     /coverage="96.0"
                     /evalue="4.31e-177"
ORIGIN
        1 gccaaagctg cggcgagcgg ttcctgctct ttcaaagacg atttcattgc ctgatttcgt
       61 caagggcgag cgtagcgagt gccatttacc cttgacgaca ctccggataa aacaggcttg
      121 gcagtggtgg ggggtggcgc gaagcgacgc cttccacctg ccagaccgcc gttgattttg
      181 accttctggc agcttgctgc ccgaacgatt ttgcgtagca aagtctagtg agtttacact
      241 atcatcttga tagtggcccg ccggaggtat cgcaatgcgc tcccccgcct ggctgaatga
      301 atgccggaat caggggggaa catggcgaac tacgcgataa tgcgctgcaa aaagctgacg
      361 ggaatgggaa gcgtggccag cgcgttgcag cactgctacc gagagcgtga gacgccgaac
      421 gctaacgctg aacggacacc agaaaactac tgctctgtgt ccaaatcaac ggatcaggca
      481 atggggcgtg tgcgggagct gctgccggaa aaacgccgta aagatgcggt actggccgtt
      541 gaatacgtta tgacagcaag ccctgagtgg tggaaagagg ccacaccacg gcagcaggcg
      601 gagtttttcg cccgctctga gcaatggctg gagaagaaat acgggaagga tcgtgttgtg
      661 gcggcggtgg ttcaccggga cgaggccacc ccgcacctgt ctgcgttcgt ggtgccattg
      721 acgcaggatg gacggctgtc cgcgaaagag tttattggcg ggcggtcaaa gatgcgtgaa
      781 gatcagagca cctacgctga atcagtaaaa aaactgggtc ttgagcgcgg catcgaggga
      841 agccgcgcca cgcatcagac agttcagcac tactacgaat cgataaaccg tggcacccgt
      901 agccaggtat cgatctcgcc ggaggcgctg gagccgcgtg tattgcggaa gggcattttc
      961 accaaagacg tggaagatca ggcagccata gccaaacgtc taagccacgc agtgaacgat
     1021 ggctttgcag gaaccatcgc tatggcctct cagagcgccc agaacgcaaa aagagcgcgt
     1081 gagctacaga agatatggat gcgcagcaga aacgcctaca gagcgtcaca gagcctttta
     1141 aggggctttc cagagagcag atgaccgaaa tcctgatgat ggctcagcgg tttaagcagc
     1201 aaaaccagga aaaggaaaaa cagcagcgta ttgaacgtga aaaacagcga cagacgcgat
     1261 cgcgtggcat gggtggtatg gagcgttagt tttgatgcga tgcaggatag ggctaacgcc
     1321 gcaaagtgac gtcatttgcg gcattcacca cgaaaaaatt aatcattcag atggtaatta
     1381 tgttttttta aaatagaaaa agctgcgttt ttctgctttg gtgacaaacc cttagttgag
     1441 tatttattac cgctagttct acgatatatg ctgtaaacac tgcttaaaaa agtacgctcc
     1501 caaacagtca gaacctcatt cttttttagt gaatcatgca gcattagcct gtctacattt
     1561 ttaaaaagtt tatcttcagc attcatcatc cattaccttc gtctgcaccg ttcttccatt
     1621 ggtgtcataa ctgatcttta actcaatttt ctttcttctt tcgtaaattg atggccagtc
     1681 tccccgtgca aaaagaaagg ggttagcttc aagttcccca cgccctgccc ggaagaatat
     1741 ttgtttatct acaagagccg caattatgtt tttaaaggtc tgatattcaa tatgaaccct
     1801 gggctgtatg gttttttcaa atctagtttt tgatacagac acaaacccat cccatccaat
     1861 cagcattgcc aattgtaaaa gcacatcatt ttgagatttt gataaaccat gaatcatcgc
     1921 aatgtctgca atgtatagct tgacgtaggg aggttcggct gggatggaaa aggtctcgat
     1981 cttagagtct tgttttacct caccgttttc atccatgtat gtatgccgtt caagcctaga
     2041 aatctttttc gtattcactc cagccccacg tttcgccatg atttagtact cgcatttaag
     2101 tactaaagag acatacagtc aagtcctttg tatcaagtac gattagtact tgaggtgagt
     2161 acgattagtc cttgaggtga gtacgattag tacttgaggt gagtacgatt ggtacttgag
     2221 gtgagtacga ttcttcgctc tagcccttgg tacaagcggg ttcccatatc tatatctata
     2281 tattaagggg gaaaataagg gctgaaaaac ggcattttta gacactgaaa aactgccttt
     2341 ttttagcaag gagtgtacgc atttctatgt acggggagtg gaaattccgc ttcctcgctt
     2401 tggctgttcg ttcctcgtcg cgttgctcct gcggtacgcc tca
//
