LOCUS       NZ_CP060897             2959 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC22 plasmid pEC22-6, complete sequence.
ACCESSION   NZ_CP060897
VERSION     NZ_CP060897.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904664
            Assembly: GCF_022558725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558725.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:20:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,946
            Genes (coding)                    :: 4,682
            CDSs (with protein)               :: 4,682
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 87 of 264
            Pseudo Genes (incomplete)         :: 179 of 264
            Pseudo Genes (internal stop)      :: 57 of 264
            Pseudo Genes (multiple problems)  :: 53 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060897.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC22"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC22-6"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-07-11"
                     /collected_by="Long Sun"
     gene            join(2634..2959,1..130)
                     /locus_tag="H9207_RS25150"
                     /old_locus_tag="H9207_24930"
     CDS             join(2634..2959,1..130)
                     /locus_tag="H9207_RS25150"
                     /old_locus_tag="H9207_24930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061350436.1"
                     /translation="MTTKFNKFELHSAFRDGKLNEKHFVDLPYLKEEQLRALASFLGAI
                     TREEPLPGKNKPSWLDDDSIKISGTDAYEDGNYWHYHCGPYPESVKLRSMTFNLGMNVA
                     GLTSAEVIHYQKLNDGTVFVVGFSPTHEPFPKSDAPHYNPLFTESDD"
                     /name="hypothetical protein"
     gene            complement(1659..2237)
                     /locus_tag="H9207_RS25155"
                     /old_locus_tag="H9207_24935"
     CDS             complement(1659..2237)
                     /locus_tag="H9207_RS25155"
                     /old_locus_tag="H9207_24935"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_123906182.1"
                     /translation="MKSEIELKKTEHLSVEIPERILAIWGRSILNSGWTSVPNELLKNQ
                     SKLGIGNTELVLLINLISFMHHSDARVYPSISLLCERMNQDRRTIQRNLNKLVEMDILR
                     IKIRSTGKNSKGMTNLYDLTPLMLKLINLKIPSLNTPDEKHTCPKCGKIAISREEITKE
                     FGFRTDTNGKMRTQSWCKDCRGKKVADLP"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(353..846)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.237"
                     /coverage="96.0"
                     /evalue="2.76e-175"
ORIGIN
        1 agaagtgatc cactatcaaa aactcaacga tggcacggtg tttgtcgtag gcttctcccc
       61 tacacatgag cccttcccta agtctgatgc gccgcattac aaccctctct tcaccgaatc
      121 agatgattaa atatctgaat agtggctggt aaggatatgt tgagattttt ttctctcagc
      181 tgtagtctct tttcctgaaa cgaaaaaacc accctggcag gtggtttttc gaaggttagt
      241 taatcctggc agattctcta accgtggtaa cagtcttgtg cgagacatgt caccaaatct
      301 gtcctttcag tgtagccgca gtcaggccac cacttcaaga actctcgata catctctcgc
      361 acatcctgtt taccagtggc tgctgccagt ggcgctttgt cgtgtctttc cgggttggac
      421 tcaagttgat agttaccgga aaaggcgcag cagtcggact gaacgggggg ttcgtgcata
      481 cagtccagct tggagcgaac tgccttcccg gaatcgagtg tcaggcgtgg aatgagataa
      541 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
      601 gagggagcca ccagggggaa acgcctggta tctttaagtc ctgtcgggtt tcgccaccac
      661 tgatttgagc gtcagattct gtgatgcttg tcaggggggc ggagcctatg gaaaatcggc
      721 aacgccgcgg cgtttttttc cccctcatcg tccctcatgg cttcatcttt tgatgtgcct
      781 gttccctccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      841 gaatatcttc gtacttatct ttggcaccgt acacgccata gctgcatttt taatgcgatg
      901 caggataggg ctaacgccgt aaagtgacgt cacctgatac ttctggatta caacagttaa
      961 agcagatgac aggatatgtt ttgtgttcac cgccagtttg tcagcgctac aaaacagcgg
     1021 gacaagatgt gtttttggag taccgcccat actccaaaac gctatgacca tgctctgccc
     1081 gataccctta gcaatcagtg tttgcaatct gtcccgaacc tgtttaggaa gtgtttgcaa
     1141 tctgtcccga atctgtttag gaagtgtttg caatctgtcc cgaatctgtt taggaagtgt
     1201 ttgcaacctg tcccgaatct gtttgctcag tgtttatagc ctgttttctc actgatttac
     1261 tctgcatgtc caaggcacgt tagcgatgta tacatcgccg cgccgcaagg gtgccccttc
     1321 cgaatcccgt tcatcactgc gtgctgatta atccacgtta aatccgaccc ccttagcaat
     1381 aaatccagcc atgcctgacg gcctggccaa cgcctcacgg ccgcacagta aactgcgcac
     1441 ccgtgaggcg ttattcgtga ggttaagtgg tgcttttgga cgtcctgccg gattttagcc
     1501 gctaaaatcc cggccatgca tgtgtgtgca tagccatgca gactcgcaga aatcatcagg
     1561 cgcgtttttt cacgatatag gtatagggaa gtgccggaaa cagtcatccc gccgcagaaa
     1621 tgcgctgagg gcgtctgagg gggtgcggcc aacggatgtt atggtaaatc cgcaactttc
     1681 ttgcctcgac aatctttgca ccaactttgc gttctcatct tcccattagt gtcagttctg
     1741 aatccaaact cttttgttat ttcttctctt gatatggcta ttttcccgca ttttggacac
     1801 gtatgttttt catcgggcgt atttaaagat ggtattttta ggttgattag cttcagcatt
     1861 aaaggggtga ggtcatacaa gttagtcatt cctttggagt ttttaccggt ggaacgtatt
     1921 tttatcctca gaatgtccat ttctactaac ttgtttaaat ttctctgaat tgttcgtctg
     1981 tcttgattca ttctttcgca tagaagcgaa attgatgggt acactcttgc gtcagaatga
     2041 tgcatgaaag agatcaggtt aatcaaaagt acaagctctg tgtttcctat tcctagctta
     2101 gattggtttt ttaatagttc attgggaacg gacgtccagc cagagtttaa aatgctgcgc
     2161 ccccatatgg ctaagattct ttcggggatc tctactgata agtgttctgt tttttttagt
     2221 tcgatttctg atttcaactt cggccaccaa tcataataac aatacctata tatataggta
     2281 ttgttattat gattaattat tcgggcggca tcaatgccgc ccttgtattt gaccaaaaag
     2341 gcctaatgtc tagcaccggg gaaagacctc tcttaccatc gaattgagca agattttatc
     2401 ttcccagatt tcttcaaaca taggactgac atgttggcaa acgtacgatt tgataatcac
     2461 agctgcgata cagaacgtcg tcagcagtac aaatctcgtg ctgacgaagt gctgctctcg
     2521 cgttcataca agaatcagac gcatgaagcg ttaaaatgcg attctccgaa gtctgatgaa
     2581 tcgatctttg atatgctatc tgcttactca gtaaggtaag tggaaagtgt ttgatgacaa
     2641 ccaaattcaa taagtttgag cttcattcag ctttccgaga cgggaagctg aatgaaaagc
     2701 atttcgtaga tttaccctac ctcaaagaag aacaactccg ggcattagcc tctttcttgg
     2761 gtgcgataac tcgtgaagag cctcttccag ggaaaaacaa gccttcatgg cttgatgatg
     2821 attccattaa aatttccggt actgatgctt acgaagacgg gaactactgg cattatcatt
     2881 gcggtccata tccagaatcg gtcaaactta gatcgatgac ttttaacttg ggtatgaacg
     2941 ttgctggact aacctccgc
//
