LOCUS       NZ_CP060895            11198 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC22 plasmid pEC22-4, complete sequence.
ACCESSION   NZ_CP060895
VERSION     NZ_CP060895.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904664
            Assembly: GCF_022558725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558725.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:20:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,946
            Genes (coding)                    :: 4,682
            CDSs (with protein)               :: 4,682
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 87 of 264
            Pseudo Genes (incomplete)         :: 179 of 264
            Pseudo Genes (internal stop)      :: 57 of 264
            Pseudo Genes (multiple problems)  :: 53 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060895.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC22"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC22-4"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-07-11"
                     /collected_by="Long Sun"
     gene            join(9998..11198,1..371)
                     /locus_tag="H9207_RS25045"
                     /old_locus_tag="H9207_24825"
     CDS             join(9998..11198,1..371)
                     /locus_tag="H9207_RS25045"
                     /old_locus_tag="H9207_24825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062821.1"
                     /translation="MSNHEENSGAAADNVTPITAAPSPLSLKLGDALFSVLSVSADWSG
                     DYRAQFELYGLDVKAINSAVGTAVWHAGKGRFLSVLNGSLTEFDKGDGMKLLEDSCGKF
                     WHRTDAFIARLTDLKIKTDDKVTKACIDMARAVRQAVAEFIMLRRQVAVVRLDVDMFAT
                     APRVELVGETVTFVRPHAPYPVANADSDVVADWLVHFPQCHEFLDALVAARFASSRKNA
                     YLFFRAQSDWGKGLLFGAGGVLSRLGATVELSEGELLNILSGANSGVTASHFMGALALI
                     VNECTRVTKKHFRLEESLALTPKYLTTQCVNLYMKIFTSADPIPGLSDSDGIDPQVANR
                     FSMLDLQGDIKTRPLFLSDKGRYVDSLTSFFAAELNARVASYQAKGFETARRDADHVLA
                     AFTTAHGLANAAGLITDAYDEIRAEWVAFVHGRVADGHPDFLTFESKAGLVLRSPVGCW
                     GKFLDWYVDKSEPLRRARLMHDRDLIIGCAKKYRDVGGAAEGKGVLIPFVSGSMPDNVH
                     RFRYKN"
                     /name="hypothetical protein"
     gene            complement(990..1457)
                     /locus_tag="H9207_RS25050"
                     /old_locus_tag="H9207_24830"
     CDS             complement(990..1457)
                     /locus_tag="H9207_RS25050"
                     /old_locus_tag="H9207_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_101998987.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRDWAKARA
                     DAPSNVLSIEEMRERAQRAVEATPQAAVRRLKNHFVQRQYELLQRHASDLQALRSASLQ
                     AMLEKDATMVKAISGLMTSLLKAQERERLIYEIKPGTEADIMRQGMNRHQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1675..1929
                     /locus_tag="H9207_RS25055"
                     /old_locus_tag="H9207_24835"
     CDS             1675..1929
                     /locus_tag="H9207_RS25055"
                     /old_locus_tag="H9207_24835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162734320.1"
                     /translation="MLLTPVRLSLWSCLPCVKADDADGAVSPCLVAEGSLVLASAVGGA
                     MTRLAKLADLAVFDAQARVAGRGGLLYALHVIKPLTGGW"
                     /name="hypothetical protein"
     gene            complement(2010..2597)
                     /locus_tag="H9207_RS25060"
                     /old_locus_tag="H9207_24840"
     CDS             complement(2010..2597)
                     /locus_tag="H9207_RS25060"
                     /old_locus_tag="H9207_24840"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_064186842.1"
                     /translation="MNKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVDLS
                     SAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            2857..3516
                     /locus_tag="H9207_RS25065"
                     /old_locus_tag="H9207_24845"
     CDS             2857..3516
                     /locus_tag="H9207_RS25065"
                     /old_locus_tag="H9207_24845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_112918731.1"
                     /translation="MTEIKTECPDAAWLRTTLAEMAGDAFPVYDLPSLRVDPNSSTQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAEADGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            3699..4088
                     /locus_tag="H9207_RS25070"
                     /old_locus_tag="H9207_24850"
     CDS             3699..4088
                     /locus_tag="H9207_RS25070"
                     /old_locus_tag="H9207_24850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015768.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            4081..4443
                     /locus_tag="H9207_RS25075"
                     /old_locus_tag="H9207_24855"
     CDS             4081..4443
                     /locus_tag="H9207_RS25075"
                     /old_locus_tag="H9207_24855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186829.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
     gene            4436..4621
                     /locus_tag="H9207_RS25080"
                     /old_locus_tag="H9207_24860"
     CDS             4436..4621
                     /locus_tag="H9207_RS25080"
                     /old_locus_tag="H9207_24860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162780107.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRREQPRSTGR"
                     /name="hypothetical protein"
     gene            complement(4641..5282)
                     /locus_tag="H9207_RS25085"
                     /old_locus_tag="H9207_24865"
     CDS             complement(4641..5282)
                     /locus_tag="H9207_RS25085"
                     /old_locus_tag="H9207_24865"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_217823325.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQIAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(5279..6568)
                     /locus_tag="H9207_RS25090"
                     /old_locus_tag="H9207_24870"
     CDS             complement(5279..6568)
                     /locus_tag="H9207_RS25090"
                     /old_locus_tag="H9207_24870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062810.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            6924..7133
                     /locus_tag="H9207_RS25095"
                     /old_locus_tag="H9207_24875"
     CDS             6924..7133
                     /locus_tag="H9207_RS25095"
                     /old_locus_tag="H9207_24875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            7120..8292
                     /locus_tag="H9207_RS25100"
                     /old_locus_tag="H9207_24880"
     CDS             7120..8292
                     /locus_tag="H9207_RS25100"
                     /old_locus_tag="H9207_24880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062813.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGFQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPVPTI
                     PGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            8523..8981
                     /locus_tag="H9207_RS25105"
                     /old_locus_tag="H9207_24885"
     CDS             8523..8981
                     /locus_tag="H9207_RS25105"
                     /old_locus_tag="H9207_24885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062815.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSIDGFAHGSLPNFSPLPRQ
                     QTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     MVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLQWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(8999..9487)
                     /locus_tag="H9207_RS25110"
                     /old_locus_tag="H9207_24890"
     CDS             complement(8999..9487)
                     /locus_tag="H9207_RS25110"
                     /old_locus_tag="H9207_24890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062818.1"
                     /translation="MREKTRTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKDARQSAPQTARQSALQNARQIVPRGADPRFQ
                     AVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYRHKLRERGLI"
                     /name="hypothetical protein"
ORIGIN
        1 tcaccactgc gcatgggttg gcgaatgctg ccgggctgat caccgacgcg tatgacgaaa
       61 ttcgggctga gtgggtggcg ttcgtccatg ggcgtgttgc tgatgggcac ccggacttcc
      121 tcaccttcga atcaaaggct ggtttggttc tccgctctcc tgtcggatgc tggggtaagt
      181 tcctcgactg gtacgtggac aaaagcgaac cgctgcgccg tgctcgcctg atgcatgacc
      241 gcgatctgat catcggctgc gcgaaaaaat accgcgatgt tggtggcgct gctgaaggca
      301 agggggtgct catcccgttc gtgtccggct cgatgcccga caacgtccac cggttccggt
      361 acaaaaattg aacaataacc cgcttcggcg ggttttttta tggcttggaa cctcgctgga
      421 acctcgctgg aacctcactg gaaccttctt aaacccgcgc cattgctggt tggaaccttt
      481 ggaacctgat tttctaacct tctctctcca ataacgaaag ctctctcaat ttccctgatt
      541 actcttttta aaaaataggt tccaaggttc catcgttgat tttaaaagat tttttaaggt
      601 tccaaggcgg ttccaaggcg gttccacaag gttccaaggc ggttccagct caattcctgc
      661 gcagttgacg cgccgcagct gcaccccgtt ggggatgcac tttgcgtgtc ttggtcgatc
      721 ggtggtttgg gtggcgctga gggcacgagc tgtcgctcgc gctgggtggt cgtctgttag
      781 ttctgcgtga gggcgtgccc tcgcgccctc tgcgttatga gtggaatgcg tagctgcagc
      841 tgcgcgtttg gttcgtccgg gcatcaaaac ggggcttctc gcgcacgcga gcggtaacaa
      901 ttgcgtaacg tgcggatccg cgtattccgg gggctcccgg gttttctgcg gcggtaaaac
      961 ggcggtaaaa ggcggctttc ccgccgtttt cagctctggt ggcggttcat gccctgccgc
     1021 atgatgtcgg cctcggtgcc tggcttgatc tcgtagatga ggcgctcgcg ttcctgcgct
     1081 ttaagcaggg aggtcatgag gccgctgatc gctttgacca tggtggcgtc cttctccagc
     1141 atggcctgta gcgatgcgct gcgtagggct tgcaggtcgc tcgcgtggcg ctggagtagc
     1201 tcgtactggc gctgcacaaa gtggtttttg aggcgtcgga ctgcggcctg tggggtggcc
     1261 tcgacggctc gctgtgcgcg ttcgcgcatc tcttcgatgc tgaggacgtt gctgggggcg
     1321 tcggcgcggg ctttcgccca atctctgacg gtgcggggtg ggatccccat ggtgcgtgcg
     1381 acggtggcac tggttgcgcc gtcctgctcc agtagcctga gcgcctgggc tttctgttcg
     1441 gtggtgtatt tcttcatggt tcctgctccg gttggtttct ctcccgtatt gaaacgtcac
     1501 gcgctgaaaa tggcgggggt ttttgggggg tacacggcgt ttgtgcgtgc gtggtcgtgc
     1561 ggttgggtgt ttttgtggct gaggcgctta gaatggctct ggtgcgttct ggtgaagggg
     1621 gcgcttccgc gcccctgtaa gtggttcgta atggcgtaag gcttttgtta ttgcttgctg
     1681 cttacacctg taaggctttc gttatggtca tgcctcccat gcgtaaaggc tgatgatgct
     1741 gacggtgcgg tatcgccctg cctggtcgcg gagggtagtc tggtactggc ctcggcggtt
     1801 ggcggcgcaa tgacgcggct tgccaaactg gcggatctcg ccgtgttcga tgctcaggcc
     1861 cgggtagcgg gcaggggtgg tctgttgtat gctctgcatg tcattaaacc tctaacgggt
     1921 ggttggtgat gcccgggttg gctctcacac ttccccgggc ctgggttgcg gcggtgactt
     1981 tagcgagtac caccgccgcg atctctctct tactccggcg cgaccgcgtc gaccttctca
     2041 agatggcggt aaatcgcgct ccggcttagg tttagctgtt tggctatggt ggcgatcggc
     2101 actcccatgg cgcggcgcat gacaatctcc ccagcttcct tcgctccgat cttggtcggg
     2161 cgtccgaact tctggcctcg gctctttgct gcctcgatac ctgctgcctg gcgttcgagg
     2221 atgagcttgc gttccatctg ggcgaagctg gccattacgc cgagtaccgc ctgtccgacg
     2281 gcgctgctca ggtcgacgcc ctcgcggatg ctgaccaggg tgatcttccg cttctccagc
     2341 tcctcgacca gctcgagggt gtccctggcg ttcctgccga tgcgatcgat cgcgtaaatg
     2401 accagggtgt cgccctcctg cagcaactgg ttcaagagga tgccgagggc agggcgctcg
     2461 cgtggtggta ccgtcccgct gatcccgtcg tcggcaagga tggaggcggt tgggaaccgg
     2521 gcgatgatgg cgtctcgctg gctgttgctg gtttgttcgt cggttgatac gcgaacgtaa
     2581 ccgtatgttt tgttcacttt tttctctcct ctgaccgata cgtctctttc gacacaacat
     2641 gccacaactg tatcgaaagt ctatcgctaa agtgtctttt tcgtcgttct gttgggtgtc
     2701 ctgaaatgta ggggattagc gacggcctcc aggcggctcc ccgcgctggg cgagagccct
     2761 gcgccctctg cgttgatgtc tgcgctgcgt cgtgacggca tgctgtctga gcggcgtttc
     2821 ttgccgcttt gctgataact tgctgagaac caaaccatga ccgaaatcaa aaccgaatgc
     2881 ccggacgcgg cctggctgcg taccacgctt gctgaaatgg cgggtgatgc gttccctgtg
     2941 tatgacctcc cgtccctgcg ggtagacccg aactcctcca ctcagctttc ggcgctcgct
     3001 gatcgtcagg ctgcgcgtga aatgcgtcag gccgcttccg acgtagaggc gcgacggctg
     3061 gacgccgctc gagtggtgga agggctcaag accgaggccg aacggctgcg gggcttgatc
     3121 gccgacggca aggctgcgct gcgtgctggc gagccggtga gccccgacgc gggtgttgcg
     3181 tcgttcctgc tgccggacat cgaggccgag ctggttgttg ctgaggctgc tgaggccgac
     3241 gtggcccggg agcgtgacac cctgctgcag gatgccgatc gccgtgacgc ggcggctgcg
     3301 ctggcgctgt tcaactgggc gcactccgtt cgcgtgcagc gtatcgagct gcttctcaag
     3361 ctggcgatgg acgaggcgac gacgctcgct gaagccgacg gcgggcgcgg cctgtaccgt
     3421 accgtgatcg cgcctgaccg ccgtcttaac caaattttcg ccacgcaggg cgctattgaa
     3481 atcctgcgcc gtaaccgtgg catggagggt gtgtaatgtc tgctttcaaa ctcctgtgtg
     3541 accggcttcg caatgaggcg acgctggcaa aatctgacgg ggccggcgct ttcaaaaagc
     3601 aatctctgga tcccgacctg aagggcggca agaagggcaa aaagcccgcc ggggagctgg
     3661 aggaggacga ggcgatcgcg tctctccacc gtaagcgcat gaacgacgtt aaaggttgca
     3721 agacgctgac caaatccacc ggcgctgatg actttggcct ccctccgctg tcgtcggtgg
     3781 tcgagacagt ggtggagaca ggcggcgcag cgcagacgtt cgcgccgatc ggggctccgg
     3841 tggcgtctcc gaccgaagcg gttcaccgtg cgcgtgcgca gtccatccag gacgcggcgt
     3901 tcctgaacga gatgaaagcg gcgggtgtgg caccggctga cctggtgtta cttgacggcg
     3961 tgttgacgtc ctctcgtggg atgccagacg cggcgcgtgc tccggcgttt gccgaggtgc
     4021 gtcgtcaggt tcaggcgggt ggcatgacgc tgtcaccggc tgcgcgtggt attgactgga
     4081 atgcgtaagc tgccgcgtca cctgcagggc gaggtgttga tgccgctgtc cagcaggatc
     4141 acgcgctcgc aacggctgga gctgatctgg ctgttgcccg ttctgcggct ccacccgggc
     4201 gatcgatcgg tcactgagcg gcgggcgctt ggcaggttcc ggggatcgct cgctgcgggt
     4261 ggccagacgg atgctttcgt ccgttcggat gcgtctctgt acgcgctggg actgcggttc
     4321 cagcgcaagc gcacccggga gctggttgct tcggatcccg atgttgctgc ggctggcgag
     4381 gcgctggagc agcgtgcaaa cggcgtgaac ctgtccgaga cgttcggggg tggcgatgag
     4441 tgaatttgct gtctcgcgcc tggtcattcc tgaggtggtg atccccgacg gggtcattgc
     4501 tgcccgacgg gcaaggggac aggctcaacg tgtcgcagct gctcgccgtg ctgcagagcg
     4561 tcgtgctgcg cctacgtggc gctgggtgag acgggaacag ccccggtcga cagggcggtg
     4621 aaacggtgag acggggcgcg ttatgcgccc tttttttcgc ccttcgcctg gcgtgccttg
     4681 cgccggaatg ccttgatctc tgtcaggcgg gctttggcgt tttcggcctc ctccggggtt
     4741 gctggttcgg tggggttgcc gtccaggtcg tagcgcatgc actggtcgat gaggctctgc
     4801 tggtacgagc tggtacccgt gacaatgccc aggatgcgac ggacggcttt gcggtctgcc
     4861 tctggcggca tgtccagctc tgcgcctcgg gtcaggatct gctcgatgat gccctttgcc
     4921 atggggcgac ggtcaactcc ccgggtcagt aagtcaggcc agtgctcttt caggaatgct
     4981 ttggtcaccc ggttggccag cgcctttttg gacttgttgg cgatcgcgat gtcctgctgg
     5041 cggcggtcgt tctcctcctg tctgcggatc ccctcggcga tctgctcggg cgtctttttc
     5101 ggctttgccg ctgtgggctt ctgtgcgggc ttctgcgggg ctttcgcttt tggctgtacc
     5161 ttcgccttgc tggtagtccg ttcggctggc tgtggctcag atttcgcggc cttttccggc
     5221 gggacgtaga ccgtgcggtt gcgtcgcttg atggtcagga tcgggcgggt gggttcggtc
     5281 ataaatctct ctcaggtctg ttggtctgga tgtggtcggc aagggcgatc agggtggcgg
     5341 cgtcgccgcg gtcccgatcg atgcgccgga tgatgtcgtt gatgtagtcc cagcgcccga
     5401 cggcggcaag gacggaaagg gtgaggcggt tgaaccgact ctctggtacc cggtcgaggg
     5461 tcagggggat gcgcttgcgg gggcgtcctg gaccgcgccc tgtccctggt cttttgagca
     5521 gctgcggctg ctccctctgc ggcaaaataa cggggatctt cgccaggctg cgcgtctcgt
     5581 tctgcgtgaa ctcgggcacc gcgatgtgct cgtctgtcca tgtcctgcct ccgggcctgg
     5641 ctgatgcgct ggagtcgcag gacgggcagg agacgacgat ctcccgtcgc ggcttgccct
     5701 tgaccggtgc cttgctgatc accaggggcg cggctttgca atcggagcag accgacgctg
     5761 tccagaccgc gttgtctggc agggtggcgt tccggtactg gtgcgcggcg tcatgcggtg
     5821 tctggaagct gaccgtctga aacgtggggg cgacgctgag gggtgccgcc catggctggc
     5881 acctgggaca agcaagctcc acctctccgg cgtgcccgtc gccaccgttc cagcgcaggg
     5941 gctcgcggtg ttcgtggcag tagtacacga aaccgtcgcg gcgtgtttgc ccgaccgggc
     6001 ctggtcggcg atcgtggaac cgtgcgcccg ggttggctcg catggcgcgg acgagcgcct
     6061 cgccctggtg gatgccgtac cagacctgcc agccatcgcc gatgccgatc aggaacggcg
     6121 agacgaacgg tggggtgcgg ttgactctcc cgcgtgcgtt ggctccgatg gtggcttcgc
     6181 gttcgcggtt ccagcaggag acgcacaggc tcgaaccggg cttattgggg gcgatcaggc
     6241 ggttgtcttt ccggctgcaa cgggggcaga gcttacgggt gtctgccggg gtggtcagtg
     6301 gtgcgtcagg gggctgttcg gtaccgaccc aaagcgcgag gccagtgggg caccggtggc
     6361 actcgaggtg ggggttttca atcgtgcgat cgaggtggct ctgctcataa atctgtcggc
     6421 atgcctcgac ccggagggag cacccgccgg ggcaggtgac gaacaaaacg ccggggagct
     6481 gctcggggaa tttttcagtc gcgcttacgc gttcaagtgc tgccgggttc ggctgcgtcg
     6541 attcgttcgt gtcgttatcg tcgttcactg ctccccctgc gtcgaaatgg tcgccagaca
     6601 gcgccagcac tggctgtgcg tcttttttag tatctcacaa aaaacgccac tttggcgtta
     6661 attgtgttac gcttgtctta ctgattcctg acgagcagtt gggaaacatg aaaccggatc
     6721 gcccggtgcc gctttccctc ctccccaacg aggcggggtt gtaaacgagg atgcgcgctt
     6781 cgtccttggc aggtgcgagt aagcgagaag ggctgctgtc ctgccgctat gcggtaagtc
     6841 ctcaagggcg gctttatcga taatcgtcac tcccgccccc cgatgcccgt taagctgagg
     6901 ggggcaccta tctggtactc ccaatgaaaa aacgaatcta taccgctgac ctcgctgcgc
     6961 tgctgaacgt gtcgctgcgc acgctctcgc ggctaagttc gcgggggatc gtgccgactc
     7021 cggcagggcg ctccctccgt ggcgggcatt actggctggc tggcaatccc gagatcaaac
     7081 gcttcgttgc cgaccagcgt cgtccgggga gggctgctga tggcgttcgt tgatttcgac
     7141 actacccatc tgaaagagga tccgggcctg ttctggcgtg ccgtatcggc tgcgggtgct
     7201 ccggctgatc gagacgaatg ggtgcgtctc tgcctggctc ttggttgctg ggcgtctctc
     7261 tgcggcgagg acgggctggc agctatcgag gcctggtcca ctcgacatgg gcgaggcgag
     7321 aatcgccatc gccagcagta caggtcgttt ttaaaaggcg acctggatac attcgccgct
     7381 caggctttcc tcaatttctc cggcgtcgaa tggcgcaacg gtcgcgcttc tgtccagtcc
     7441 gacgtcgatc gcgctgaacg gctgcggttg tcccgtgagg ccacagcgaa ggcaaaggct
     7501 gaacgcgaca agaagggtga tgctgctgcg cgtgctgcgg tcaagctgct ggcgaaggga
     7561 ggggatcctg cgtctacccc atactggcgc aaaaagaccg ctctggcagc tgcgggtggc
     7621 gtccagctgc ccgccctcag cacagatctt ggtgttggcg ggatgtttcg atcgttcaat
     7681 ccgcgtgctt actggtctga ttgcctcctg attccggttt acaagttcga cgccgcccgt
     7741 cagctgtatg ttgacggcct ggaggccatc tgcgggcgtt gctcggctga gggtttccag
     7801 ctcactggct cgaagttctc tccggcgggt acccgcaagc agggcggctt ctacccgatg
     7861 ccggggttca tggacgtgct cgacggctcc ccgcacgcgc cctactcgcc tctgttcgtc
     7921 tgtgaggggt tcgctacggc gttcgccctg caccacctga gccgtggcaa ggtggcggtc
     7981 tgcgctgcgt tctcgatcgc cgggttcggc agctgcgtgg cgtcgctgcg tcggttctgg
     8041 ccagatgttg atgtgatgct ggtaccggat cacggcgaag cgtaccgcct ggcgcataaa
     8101 gcggcctatg gcgatgagcg agagccggtc ccgacaattc caggcgcggc ggttgtgtcg
     8161 ttcgacggcc tcgatcggtt cactccccgg gataacctcg actggttcga cgtgctggtt
     8221 gaggaagggg aagatcgggc acgtgagctg ctccggtatg cggttcgtca tgcccggctt
     8281 gagcggcgtt gattgacgtg tccgaatggt gcgcctttcg ggacacacct cctgctgccg
     8341 ctgccccgga ttttgcgacc gactttcgag acactatcga cgcttttttg catagacgga
     8401 ttaaagcgac gcaccgccgc tgcggtcgtg acgctatcct gcagctggtt tggtttccag
     8461 cagcacactc aagcccggca ccgttttggt gtcgggcttt ttttcgcccg taaggagggc
     8521 acatgaaatc aaagactgaa ttacgcgctg ccgcgacggc tcgcgccctt gaggtgatcg
     8581 cttctgagat ggggggttgg tcgatcgacg ggtttgctca cggctcgctg ccgaacttct
     8641 ccccgctgcc gcgccagcag acgcaggagg gctctgtggt gctcgaacgc ccgccgttcg
     8701 actacacctg ggcgggtacc gctgcgttta ccgatcgtgc aaaccgtgcg ctgcaggtga
     8761 agctaccggc gtcgcgtgag cgtaactaca tctggctgtg cgcggtcgaa agggaggctg
     8821 tggccaccgc gctgatggtg gagtcgttca acgtgaccgg ctgcgcggcg ttcgccgggt
     8881 tgcctcctgt ggatggcatg gtgctgctga ccatggacga ggccgacgtg gagctgatca
     8941 gggcggcgat gctgcagtgg ctggatgcgg cggcggcgtg agccgtcgcc cctctgcgtc
     9001 aaatcaggcc gcgctcgcgc agcttgtggc ggtagtcccg gacgcggctg gtattccact
     9061 cctgcccggt ggcggtggtg tgcccggctg cgttcagcgc ctgggcgatc gcgggcaggg
     9121 tggcgttctc cccggtcatg ctgacgatga gctggtcggc ctcatcgctg acggcctgga
     9181 agcggggatc ggctccccgg gggactatct gtcgtgcgtt ttgtagtgcg ctttgtcgtg
     9241 ctgtttgtgg tgcagactgt cgtgcatctt tcccgcctcc ggccagcgcc ctgacctctg
     9301 accgcagcgc ctcgatggcg gtcaaaagtt tggtgtccat cccttccggc ttcgtgccga
     9361 gtccggcgac aagcgcctct atgacggcat ctgtgcgggt tactttggtt ttctccgctc
     9421 gctgctcgat gcgctcgatc aggctgacgg gcagtcgaac ggttagggtt ctggttttct
     9481 ctcgcatggg ctggcctctc cgtgtcgtgc gttttgtcgt gctgtttgtc ttgcggagtg
     9541 taatgcgttt tgtcgtgcgc gtcgaatttg acgtagtgca attgtcgtgc agggaggggt
     9601 agcggctcgg cccgtggata acttctgtgg ataacaaaat ttcgaacgtt ccggcccgtg
     9661 gataaatcgc ggaggctgcg cggctctaag atcggtgcgg gatcgcgttt cgcgtggttg
     9721 gatcgttgtg tactggtgca ggggggtatg taacggtatg atggcacggc tgaaaaagaa
     9781 aacccgtccg ggcagacggg tcgttttctt aaagctgttt tgagtttgga gctttcgcgg
     9841 cggtcaccgt ggaagacgcc ataataactc atgttgggtg tgactccagc atgaattata
     9901 cgcgatcata atgacgcgtg caaagggtgt tttccccgtt atcccccgcc gttgtaaggc
     9961 tcctcgagat tggctgttca attttgagga tgtaaaagtg tcaaatcatg aagaaaattc
    10021 cggcgctgct gccgacaacg taacgccgat caccgctgca ccgtcaccgc tgtcgctaaa
    10081 attgggcgat gcgctgtttt ctgtcttgtc ggtgtctgcg gactggtctg gtgactaccg
    10141 cgctcaattc gagctgtatg gcctggatgt aaaagctatc aactctgccg tcggcactgc
    10201 cgtctggcac gctggcaagg ggcggttcct ctctgtccta aatggctccc tgaccgagtt
    10261 cgataagggc gacggaatga agctgctgga ggactcctgc ggcaagttct ggcaccgcac
    10321 cgatgcgttt atcgcccgcc tgactgacct caaaatcaag accgacgaca aggtaaccaa
    10381 ggcgtgtatc gatatggctc gggctgttcg tcaggcagtg gctgagttca tcatgctgcg
    10441 ccgtcaggtg gcagtcgttc gcctcgacgt ggacatgttc gccacggctc ctcgcgtcga
    10501 actggtgggt gaaactgtga cgttcgtccg tcctcatgct ccctatccgg tggctaatgc
    10561 cgacagtgac gtggtggctg attggctggt tcatttccct caatgccatg agttcctcga
    10621 cgcgctggtt gcagcccgct ttgcctcaag ccgtaagaat gcctacctgt ttttccgtgc
    10681 gcagtccgat tggggtaagg ggctgctgtt tggtgctggc ggcgttctgt cacgcctggg
    10741 ggcgacggtg gagctgtccg agggggagct gctcaacatc ctgagcggtg ccaactccgg
    10801 tgtaacggct agtcacttca tgggggcgct ggcgctgatc gtcaacgaat gcaccagggt
    10861 gacgaaaaag cactttcgtc ttgaggagtc gctggctctg acgccgaagt acctcacgac
    10921 gcagtgcgtg aacctgtaca tgaaaatttt caccagtgcc gacccgatcc ccggactgtc
    10981 cgactccgac ggaattgacc cgcaggtggc gaaccgcttt tctatgctcg atctgcaggg
    11041 cgacatcaag acgcgcccgc tgttcctgtc cgacaaggga cgctatgtcg acagcctgac
    11101 gtcgttcttt gccgccgagc tgaatgcccg ggtggcgtcc tatcaggcta agggctttga
    11161 gaccgctcgc cgcgatgccg accatgttct ggctgctt
//
