LOCUS       NZ_CP060889             4091 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC24 plasmid pEC24-5, complete sequence.
ACCESSION   NZ_CP060889
VERSION     NZ_CP060889.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904665
            Assembly: GCF_022558705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558705.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:19:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,992
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,619
            CDSs (with protein)               :: 4,619
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 84 of 252
            Pseudo Genes (incomplete)         :: 163 of 252
            Pseudo Genes (internal stop)      :: 44 of 252
            Pseudo Genes (multiple problems)  :: 33 of 252
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060889.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC24"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC24-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-08-17"
                     /collected_by="Long Sun"
     gene            43..333
                     /locus_tag="H9208_RS24820"
                     /old_locus_tag="H9208_24625"
     CDS             43..333
                     /locus_tag="H9208_RS24820"
                     /old_locus_tag="H9208_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            866..1567
                     /locus_tag="H9208_RS24825"
                     /old_locus_tag="H9208_24630"
     CDS             866..1567
                     /locus_tag="H9208_RS24825"
                     /old_locus_tag="H9208_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1816..3963)
                     /gene="mobQ"
                     /locus_tag="H9208_RS24830"
                     /old_locus_tag="H9208_24635"
     CDS             complement(1816..3963)
                     /gene="mobQ"
                     /locus_tag="H9208_RS24830"
                     /old_locus_tag="H9208_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_011666798.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     KRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          3609..3963
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3980..4091
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.351"
                     /coverage="85.0"
                     /evalue="1.69e-33"
     Typing          867..1566
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1820..4005)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.863"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgcgctaaa atgcgatttt tcaccgcgca cggagttacg gaatgagtag aacactcgaa
       61 cagaagattg ccgatgctga ggccagattg cagcgtctga aggcgaagag caggagcctg
      121 gacacagcgc agaaggtcat tgtgggagcg gcattgctgg caaaggtcag gaagcctggg
      181 gaaacacaac tacgagcctg gttgcttcat ctcctgaaga cagaggtaac acggcaggct
      241 gatgtgagca ggatacagcc gttgattgac gaactgaacg cgttaccaaa accagtcccc
      301 aaagaagtgt cagaaaacga ccaacaggcg tgaatgcacc gtccgctcgc cgcagcccca
      361 gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatccgt cgccagattg
      421 agcacatacg cacggttttt cgacaggtga tatgcgctga acccgcatga atactggatt
      481 tccacagggt tgagcggggt tttgctaaag cttgcgaacc tgcttttttg taacctgttg
      541 tttttcatga ttttttgttt tgaacacccc aagttttcca caggctcgcc gttaagtttt
      601 tagatcttgt ttttaagatc ttgtttttaa tacttgtttt agggtcgaat tttcttatga
      661 aattcaagag tttagaagac tataggtaat ataatttaag gtaaaggcaa tatgatttaa
      721 ggtggatagg caatatgatt taaggtaaag gcaatatgat ttaaggtaat attcacagga
      781 aaagggatat taaaattaaa tcatattgcc tttacctaaa ggaatgttta tattttactc
      841 attaccttag gttgagggat ttacaatgtc ggatctgatt gcctacaaat ccaatgcact
      901 ggttgaggcc agctataaat tgactcttca ggaacagcga ttcttgttac tttgcattgg
      961 tcgattgaag tcgggttctg atgcagaatc cactgaattg caaaagacca tgactgttac
     1021 cgccgaagag tatttcgact cattccctga tatggggcgg aaaaatgccg aggttcagct
     1081 acaggaagca attgatcgtt tgtgggacag gtcgatcatc cttaaggacg atgagaagcg
     1141 cgaggagttc cgctggatcc agtatcgggc acagtacgct aaaggtgagg gcaaagccca
     1201 aatcactttt tcagatgcgg tcatgcctta tctgacacag ttgaaggggc aatttactag
     1261 agttgtaatt aagaacatct ccaatttatc aagaagttac agcataagaa tatacgaact
     1321 tctccagcaa ttccgcagca caggggaacg tattatcgcc ctcgatgatt ttcgctcaat
     1381 gcttgatata gagcataaat atcaaacata taagtctctg aaccagcaat tattaagacc
     1441 ttgcgttgac gaactgaatc aaaaaagcga cttggctgta acgcttgaga cgatcaagaa
     1501 ggggcgcacg gttgtagccc tgcattttcg cttcaaggaa gacaaacaga tcaagatgac
     1561 gatctagaat cgattttaag gcctctaaaa cgctctctga cgcgtttttt ttgtgtttgg
     1621 catgcgaagg tactggtgaa gtaaaaaatt gcgtcataaa cgctctggca cagtgaaaaa
     1681 agaaccccca atttttcggg ggcgggaaaa ctataaagtt gatttgtgtt atgtgtgtgc
     1741 ttcttgcatt catccctgtc gatgaacatc gacgaacata acacgatgtt aaacaaaaca
     1801 aaatattggt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg tggcgtgtgt
     1861 tcgggcatat tcatcacgtt tccattcatc ccaggtgcgc tcaaagccca gcgagcggaa
     1921 cagatgatcc ggctgttttt ctggcgtgtt gacgatagca aaaagcggtt cgctccaggt
     1981 tttcagatga aactgcataa gttccggcgc aggacgtgcc ggactgtcag catcgtggtt
     2041 taccgcttca ccatagcctg caaagcgtaa acctttgtcg gtcagtgcat tgattaccag
     2101 ctggctgttg tcggctgtgg ggacggcaaa cgcgggatac agcgtaccat taagccggac
     2161 aacaccgccc atagagtcat ccacacccat gaccagcaaa agctgaagct ggcgggtttc
     2221 acggtcgaca gaactcatta ccggcatcag agtcggtggc agacgtccct gcgtaaaatc
     2281 caccttcctt gccgccagtg atggcatggc tggctggcgg tcttctccaa gacagaaaga
     2341 ctccggccat gtcttcggct ggcggagatc gcgggtatgt tgcggccatg tctggcaaac
     2401 ctgcgtcagc agttgctcaa aggtcgcaga ggaataaaca tgttgcagtg gtggacgttg
     2461 accgatttcg cttcgctcac agatataacc actcctgtca cgcattaaat caacactctc
     2521 aaaaattctg gcgcggtatt tctccagttc gttcccccag agcgtatatt ctccgttccg
     2581 gttgtgaagc agaaggtaac aggtcatttc cccgttacct ggcatggggg cctcgccata
     2641 ccccgtaacg actgctttca gtacccctga atccataacg gcattgtcat ttctcctgag
     2701 ctgctccgcc cgacgacgtt ctgtttcctc ctgctccttc cggcgcaatt cctcttcacg
     2761 tttacgcaac tgttctcgga gatctgcaag gccagatgca ccccactgct gccactgtgt
     2821 ttcaaatgct ttttgactcg cgggaatgtc ctgtttctca accttccagt gatgctgccg
     2881 caatttttcc atgcgctggc tcaacgactg gattttttcc tgttcgcggc cgtattccgg
     2941 tgcaacccat tgcatcaggg gaatacggga aaaaccggac gaaaacggcg atgatttttt
     3001 caggctggca gcatggctca tcgcttcctt tcgttcggtg ttcagatctt cccagcgtct
     3061 gcgcgtcgcc tgcatatctg ccatcatcga catggcttta ctggtgagtt tccatgctgc
     3121 gttactgtcc gtcggcaacg cttcgatgat gtcatgcagt atgtacgaag ttgcgttgtc
     3181 aggaagctca atattcagaa ggtcagccat acggcgtgcg ggctctgtca gctcactgtt
     3241 cctgagttcg tcgcgggaaa cctgtatttc caggttaagc ctgttaatca ggcgattctg
     3301 ttcgccgcgt tctgtttcga tgccccactt ttccatcgca caggccgact ttccgaggtg
     3361 aatgccaggc gttttctcaa ccccctgtcg ctcgtatgaa cgatggtcta tctcttcctg
     3421 ataaccggcg ttcgccagcg ccctgttcgc atggtctgcc catgatcccc gccatgtttc
     3481 ggcgtgagtc cagtcattcc actcacggac tttctccccg aagccgtcag gcgtgattgc
     3541 cctcatggat agcatgatgt gcgcgtgtgg gtttgacccg cccagattgt gaaaggccac
     3601 gtcggcgatc atgccgcgac tgacaaagtt ttcccttacg aagttgagaa ctgtttcccg
     3661 tgcggcatcc tgcgccagtt cacgggggat ggcgagttcg aactcccgcg caagttgtga
     3721 gtttttccgc cgctcggctt tttcaacagc attccagagt tcggcgcggt tcgcacacca
     3781 tgctggggcg tttgctggcg tcagaatcac tgcgctggct acaccgtttt tgcgggtgta
     3841 gtcgtgaatc actccggtgc gctcatccag tattttttct ccggcacgat aggcagcaga
     3901 agcaacggcg ctgtagccct tgcttctgga aagaatcttc atgctgagat gataaattgc
     3961 catcgtgtcg ttgttgccgt tttcttttag ccccgcaggg cgcacacaaa ctcgacgaag
     4021 gagagtttgt ataagtgcgc acttcgtgat ttgctgtgca ttatgccagt tcagttttta
     4081 aactaccagt g
//
