LOCUS       NZ_CP060884             3257 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC26 plasmid pEC26-5, complete sequence.
ACCESSION   NZ_CP060884
VERSION     NZ_CP060884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904666
            Assembly: GCF_022558685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558685.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 03:59:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,995
            CDSs (total)                      :: 4,874
            Genes (coding)                    :: 4,663
            CDSs (with protein)               :: 4,663
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 79 of 211
            Pseudo Genes (incomplete)         :: 131 of 211
            Pseudo Genes (internal stop)      :: 45 of 211
            Pseudo Genes (multiple problems)  :: 41 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060884.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC26"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC26-5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-09-06"
                     /collected_by="Long Sun"
     gene            119..415
                     /locus_tag="H9209_RS24825"
                     /old_locus_tag="H9209_24565"
     CDS             119..415
                     /locus_tag="H9209_RS24825"
                     /old_locus_tag="H9209_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609270.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antitoxin"
     gene            complement(646..758)
                     /locus_tag="H9209_RS25095"
                     /pseudo=""
     CDS             complement(646..758)
                     /locus_tag="H9209_RS25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1412..1606)
                     /locus_tag="H9209_RS24830"
                     /old_locus_tag="H9209_24570"
     CDS             complement(1412..1606)
                     /locus_tag="H9209_RS24830"
                     /old_locus_tag="H9209_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2750..3031)
                     /locus_tag="H9209_RS24835"
                     /old_locus_tag="H9209_24575"
     CDS             complement(2750..3031)
                     /locus_tag="H9209_RS24835"
                     /old_locus_tag="H9209_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000586174.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          1257..1339
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          1643..1741
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1866..2121
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(687..1244)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.464"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcatcata atgtcattct tttttttagt caacaatgag ttgcgttatg cgcaatgcgc
       61 aatctgaaaa cgcaataggc ctatcttttt aaaactaata taggcttcag aggaaggtat
      121 gattaatctt gtcgatccag tacatccagg ggtttttatc cgtgagatgt ttatggaacc
      181 gtttgaaata tcagccgcgg acctgtctga aaaactgcat gtatcacctt ccactctaag
      241 tagagtgctg aatggaaaag ctgatttaag tgttgagatg gcattaaggc tttctcgagt
      301 tcttggtcgc agtgccgagt cgtggataaa catgcagact caatacagtc ttaacgcgag
      361 caggaagtca cctaatgtca ttaaggaatt agatgaattg gttcctatat tttgagaaaa
      421 gaaaaggccg ctatatgcgg cctttttctg tcttgactct caggcaactg gatcgtatcg
      481 tttctcgtaa acgaaaaaac cacccggcaa ggtggttttt tcgaaggttc agtaaccggc
      541 aagtttctga accagggtaa ctggcttgga ggagcgcagt caccaaaact tgttctttca
      601 gtttagcctt aactggcgca taacttcaag actaactcct ctaaatcagt taccagtggc
      661 tgctgccaat ggtgttttgc atgtctttcc gggttggact caagatgata gttaccggat
      721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      781 acctacactg aaccgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      901 gagccaccag ggggaaacgt ctggtatctt taagtcctgt cgggtttcgc cacctctgac
      961 ttgagcgtcg atttctgtga tgctcgtcag gggggcggag cctatggaaa aacgcctgcg
     1021 gcgcggcctt cttcctgttc ttggtctttt gctcacatgt tctttccggc cttatcccct
     1081 gattctgtgg ataaccgtgc tgccgttttt gtgtaagtca gtaccgctcg ccgcagccga
     1141 acgaccgagc gtagcgagtc agtgagcgag gaagcggaaa agcgcctgga cgcgcatttt
     1201 ctccttacgc atctgtgcgg aatttcacac ccggcatggg gcacttttca tacaatctgc
     1261 actgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcaggg
     1321 cttcgccccg acacccgctg acgcgccctg gcgggcttgt ctgctgccga aaacgctcgc
     1381 ggacaagccg tgagcgcctc cgtcagcttc gttatgttcc tgtttcactt tcggtctcaa
     1441 agcgaacctg gatgctgttc tggagttctt ccgcgagttc gtgcagtcgt tcgcacatgg
     1501 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     1561 tgataaatcg cgccatattc agcgcagttt gttgctgttt gttcaccttc cgttgttctc
     1621 cgtcagtacc gggtgtcggg tggaccctga ccaggtggta attgtaagac gttgcgggcg
     1681 acggtattac aattgcacat cctgtcctgt ttttttagga cgtatcatgg cacagcatat
     1741 caactgataa ataatccctg tgcagtgcag ccgctgttgc ataagatttc gctctgcgtc
     1801 gagtgtttct taatcaataa ttccgctgat actccggaaa aacgcgagtc tgttctcatg
     1861 cctgaaatgc ccacacccca cgcaaaaaca attttttgct gatttttctt tataaataga
     1921 gagttatgac aaattagttc ttcttgctct ctttgtgata tttaaaaaag cggtgtcggc
     1981 gcggtgttgt agctgcgcca acaccgcttt ttaggggtgg tactgactat tttcataaaa
     2041 aacatcattt tatattaggg gtgctgctag cggcgcggtg tgttttttta caggacgccc
     2101 ctgggggcgc tgctaggggt gtctgttcag atatgtagca atgagtcagg gattcacgaa
     2161 ctgaaaatgt ccgaaaatgt gtctcatggg caccgtatgc gcccaaaccg atcgccattc
     2221 actttcatgc atagctatgc agtgagctga aagcgatcct gacgcatttt tccggttttc
     2281 gcttggtaaa acatctcttt ttgcggtgtt tgcggcagaa tcgcgttcag cgcgttttag
     2341 cggtgcgcgt aatgcgacgt tatggtaaat gctgtgattt acgggatggt tttatctatg
     2401 ttgatatcaa taccacctct tatggaggtg gcttacaccg atttttcctt gttgtctgtt
     2461 tcagaacaga tagaggcttt tttggcaatt atccaaccac tattgatagt gtttattact
     2521 acttagataa ctgagccttt actgtatctg cccctgcaag caggaaccca acagttaaga
     2581 accaccccca gccattgata ccttttagcg ctagaattgc tgcgcaaatg atggctgcta
     2641 tagggactat ctggtacaga aaatacttca tataacttac cccgaggttt tgctttttta
     2701 tgagacccta aagcatcgat actgtcactt tgatgcttta aggaccttct tagtgataat
     2761 cctcgagatc cacgtcaaag acgttaggtt cttcgagctt aaacactacg cgccagtttc
     2821 cattgacctc taacgaatac tctccttgac ggtctccctt caatggatgg cagcgcaatg
     2881 cctttaaatc catcatatcc tgcaagttcg tcgctgtgct taaggctgtt aggatcagct
     2941 tcagacgttg agtaaactgt tgcggaattc ctgcgttgtt acctgtttcg gcaaacttac
     3001 gcaaaccttt atgcttgata tttatgatca ttaatcacca tcccatattt gtttagatag
     3061 aaacatcgca gtttattttt ccggttcatc cgtatttcca ttgcaaaccc cttgctctat
     3121 ttgaataaat gacgtaggtt ttacttaacc aagtcgcatt ttaatctgtt gacattggtt
     3181 tgatgcgctg tacaatgccg gggccttgaa tttcacatca agtacccaca tcataaggaa
     3241 tgattagacg caccaat
//
