LOCUS       NZ_CP060879             4067 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC27 plasmid pEC27-7, complete sequence.
ACCESSION   NZ_CP060879
VERSION     NZ_CP060879.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904667
            Assembly: GCF_022558665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558665.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:21:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,904
            Genes (coding)                    :: 4,645
            CDSs (with protein)               :: 4,645
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 88 of 259
            Pseudo Genes (incomplete)         :: 172 of 259
            Pseudo Genes (internal stop)      :: 46 of 259
            Pseudo Genes (multiple problems)  :: 39 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060879.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC27"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC27-7"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-10-16"
                     /collected_by="Long Sun"
     gene            complement(join(1973..4067,1..53))
                     /gene="mobQ"
                     /locus_tag="H9210_RS25015"
                     /old_locus_tag="H9210_24815"
     CDS             complement(join(1973..4067,1..53))
                     /gene="mobQ"
                     /locus_tag="H9210_RS25015"
                     /old_locus_tag="H9210_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_124770306.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATVRRHDRGWSQ"
                     /name="mobQ"
     gene            232..486
                     /gene="mobC"
                     /locus_tag="H9210_RS25020"
                     /old_locus_tag="H9210_24820"
     CDS             232..486
                     /gene="mobC"
                     /locus_tag="H9210_RS25020"
                     /old_locus_tag="H9210_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_118998437.1"
                     /translation="MSRTLEQKIAEAEARLQRLKVKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1018..1725
                     /locus_tag="H9210_RS25025"
                     /old_locus_tag="H9210_24825"
     CDS             1018..1725
                     /locus_tag="H9210_RS25025"
                     /old_locus_tag="H9210_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          55..225
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.419"
                     /coverage="97.0"
                     /evalue="5.65e-83"
     Typing          complement(3763..4067)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.346"
                     /coverage="100.0"
                     /evalue="1.88e-155"
     Typing          1019..1725
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1977..4066)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.718"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgtagccgc tgtttctgga gatgattttc atgctgagat gataaatcgc catttctgcc
       61 gtaatgcctt tttgtggttt tctttcagcc ccgcagggcg cacacaatcg aaacggagtg
      121 gagattgtat aagtgcgcac ttcgtgattt gccgtgcatt atgccagctc agtttttaaa
      181 ctaccagtga cgcgctaaaa tgcgattttt cactgcgcac ggagtcacga aatgagcaga
      241 acactcgaac agaagattgc cgaagctgag gccagattac agcgcctgaa ggtgaagagc
      301 cggagcctgg acacagcgca gaaggtcatt gtgggtgcag cattgctggc aaaggtcaga
      361 aagccggagg aagtgcagtt gcgagcttgg ttactccagt tcctgaaggc agaggtgaca
      421 cggcaggctg atgtgacgcg aatactgccg ctgattaacg agctggaggc gctgccagag
      481 cagtgattgt atcgtccgct cgccgcagcc cagcgaccga gcgttagcga gtgagtgggc
      541 gaggaagcgg aagtgaatca gtcgccatgt tgagcacata cgcacggttt ttcgacaggt
      601 gatatgcgct gaacccgcat gaatactgga tttccacagg gttggagggg gggggtattc
      661 ggaatcttca atcctgattt ttgcaacaca ttgtttttat tggattttta ttttcagcac
      721 cccaagttat ccacaggctc gccgttaagt ttttagatct tgtttttaag atcttgtttt
      781 taatacttgt tttagggtcg aaattcctta tagaaaacaa aatgttacaa cgctataggt
      841 gatatgattt aaggtaaagg tgatataatt taaggtgctt gggtgaggta atttaaggta
      901 aaggtgagga gatttaaggt aatattcaca ggaaaatggg gggtgaaaat ataattgctc
      961 accaatgcta aaggtgatag gtttttatct cacctcatct ttggcctggt ggttaaaatg
     1021 acatcaaacc ctctgatagc ctataagtcc aatgcgctgg ttgaggccag ttacaagcta
     1081 actttacaag aacagcgttt cttattgctc tgcataagcc gacttaactc cggtactgac
     1141 gtagcttcac ctgaattgca aaagacaatg accattacag ctgcggaata ttttgattca
     1201 ttccctgata tggggcggaa aaatgccgag gttcagctac aggaagcgat cgatcgtttg
     1261 tgggacaggt cgatcattct caaggacgac gagaagcgcg aggagttccg ctggattcag
     1321 tatcgggcac agtacgcgag gggagagggg aaagcacaaa tcactttttc tgacgcggta
     1381 atgccttatc tgacacagtt gaaggggcaa ttcaccagag tcgtcattaa aaatatttct
     1441 aatttatcaa gaagttacag cataagaata tacgaaattc tccagcaatt ccgcagtact
     1501 ggggaacgta ttatcgccct cgatgatttt aaatcatcac tgatgcttga tggtaaatat
     1561 aaagatttca aaacgttaaa cagggatcta ataaaaccct gtgttgacga actgaacaaa
     1621 aaaagcgacc tggctgtaac ggttgagacg atcaaaaagg ggcgcacggt tgtagccctg
     1681 cattttcgct tcaaggaaga caaacagatc aagatgacga tctagaatcg attttaaggc
     1741 acctaaaacg ctcactgtcg cgtttttctt gtgtcaggaa tgcgaaggta ctattgaagt
     1801 gaaaagttgc gtcaaaaacg ctctgatgca gcaaaaaaag aacccccaat ttttgggggc
     1861 ggaaaaacaa aactatgttt gcgctatgtg agtgtgcttc ttgcattcat ccctgtcgat
     1921 gaacatcggc gaacataaca cgatgttaca caaaacaaaa tattgttcac attcactgtg
     1981 accaaccgcg atcatgtctc ctcacggtgg cgtgtgttcg tgcatcttcc tcgcgccgcc
     2041 attcgtccca ggtacgatta aaacccagcg agcggaacag atgatccggc tgtttttctg
     2101 gtgtgttgac ggcagcaaaa agcggtgcct cacgggtttt cagatgaaac tgcatcagtt
     2161 ctggtgcagg tgatatgtgg ctgtctgcat cgtgatttac ggcaacacca taccctgcat
     2221 accgtaaccc cttatcggtt agtgcactga tgaccagctg gctgttatcg gctgagggaa
     2281 cggcaaacgc gggatacagc gtaccattaa gccggacaac accgcccaga gagtcatcca
     2341 cgcccatgac cagcaaaagc tgaagctggc gggtttcccg gtcgacagaa ctcatgaccg
     2401 gcatcagcgt cggtggcaga cgtccctgcg taaaatccac cttccttgct gccagtgatg
     2461 gcatggctgg ctggcggtct tctccaagac agaaagactc cggccatgtc ttcggctggc
     2521 ggagattgcg ggtatgttgc ggccatgtct ggcaaacctg cgtcagcagt tgctcaaagg
     2581 tcgcagagga ataaacacgt tgcagtggcg gacgttggcc gatttcgctt cggtcacaga
     2641 tataaccact cctgtcacgc attaaatcaa cacgctcaaa gattctggtg cggtatttct
     2701 ccagctcgtt accccagagc gtatattctc cgttccggtt atgaagcaga aggtaacagg
     2761 tcatttcccc gttaccaggc atgggggcct cgccataccc cgtaacgact gcgctcagta
     2821 cccctgaatc cataacggca ttgtcatttt tcctgagctg ctccgcgcga ctacgttcag
     2881 tatcttcctg ttccttccgg cgcaattcct cttcacgttt aagcaactgg gctctgagag
     2941 ctgcaaggcc ggattccccc cactgctgcc actgtttttc aaatgccatc tgactcgcgg
     3001 gaatgtcctg tttctccacc ttccggtaat gctgtcgcaa tttttccatt tgctggctca
     3061 gagactggat ttttccctgc tcactacggt attccggtgc cgcccattgc atcaggggaa
     3121 tacgggaaaa accggacgaa aacggcgatg attttttcag gctggcagca tggctcatcg
     3181 cttcctttcg ttcggtgttc agctcttccc agcgtctgcg cgtcgcctgc atatctgcca
     3241 tcatcgacat ggcattactg gtgagtttcc atgccgagtt actgtccttc ggcaacgctt
     3301 cgatgatgtc acgtagtgtg tacgaagttg cgttgtcagg aagcccgata ttcagaaggt
     3361 cagccacacg gcgggcaggc tctgtcagcc ctccgttcct gagttcggta cgggaaatct
     3421 gtatttccag gttaagtctg ttaatcagac gattctgttc gccgcgttct gtttcgatac
     3481 ctcgtgtttc catcgcacag gcagactttc cgaggtgaat accaggcgtt ttctcaagcc
     3541 cctgtcgctc gtatgaacga tggtctatct cttcctgata accggcgttc gccagtgccc
     3601 tgtttgcatg gtcagcccat gatgcccgcc acgtctcggc atgtgtccgg tcgttccagt
     3661 ccctgacctt tccaccgaat ccagcaggtc cgacagctct cgtggtcagc atgatgtgcg
     3721 catggggatt tgttttatcc atgtgatgga acgccacatc agcaatcatg ccctgactga
     3781 caaagttttc tctgacaaag gcgagaacgg tttcccgtgc ggcatcctgc ggcagttcgt
     3841 gggggatggc gagttcaatt tcccgcgcaa gttgtgagtt cttccggcgt tcggccttct
     3901 caacggcatt ccagagttcg gcacggttcg tacaccaggc aggggctgtc gcgggagtca
     3961 gaatcacagc ttcagccaca ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct
     4021 catcaagcat cagtgaaccg gaacgatagg cagcagaagc aacggcg
//
