LOCUS       NZ_CP060878             4886 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC27 plasmid pEC27-6, complete sequence.
ACCESSION   NZ_CP060878
VERSION     NZ_CP060878.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904667
            Assembly: GCF_022558665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558665.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:21:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,904
            Genes (coding)                    :: 4,645
            CDSs (with protein)               :: 4,645
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 88 of 259
            Pseudo Genes (incomplete)         :: 172 of 259
            Pseudo Genes (internal stop)      :: 46 of 259
            Pseudo Genes (multiple problems)  :: 39 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060878.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC27"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC27-6"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-10-16"
                     /collected_by="Long Sun"
     gene            complement(join(4724..4886,1..416))
                     /locus_tag="H9210_RS24990"
                     /old_locus_tag="H9210_24795"
     CDS             complement(join(4724..4886,1..416))
                     /locus_tag="H9210_RS24990"
                     /old_locus_tag="H9210_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021543812.1"
                     /translation="MAKLPLPPLPLPIAPPSTVKNSGSERAKKSGRRTLAAKRKTLLEE
                     LWGDELEQLKVWNRHEHDGYTTVPRTLTYISRILDYLGGTGQPLSQTYLALWCRVFDEG
                     FVEIKDKDGFAYEAGFSGQRAVTTWTGRMRKLRDLGFITTKPGTSGEFQYVILLNPLTV
                     IKELYEGKEKDERYNALVGRMQEVGAKWE"
                     /name="hypothetical protein"
     gene            1663..1854
                     /locus_tag="H9210_RS24995"
                     /old_locus_tag="H9210_24800"
     CDS             1663..1854
                     /locus_tag="H9210_RS24995"
                     /old_locus_tag="H9210_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021543809.1"
                     /translation="MTKPQKTALNMAKFIKGQTLLLLEKLNELELDAEADLCEKLHEDA
                     ELLCRTLTIRLDGHQGDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1854..2285
                     /locus_tag="H9210_RS25000"
                     /old_locus_tag="H9210_24805"
     CDS             1854..2285
                     /locus_tag="H9210_RS25000"
                     /old_locus_tag="H9210_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024249395.1"
                     /translation="MARVSISEAARLACVSRPTIYKLIKSGELSYTSVVKHGKAVKVID
                     TTELIRVFGSLSFDNERDHAVVKFDDRSTGVNSDFLQNLQHQVALLQAENAGLKDAVNA
                     RDEHINSLRQAMQLLEHKQGTADPQHVRAKSWWQFWKFR"
                     /name="helix-turn-helix domain-containing protein"
     gene            3523..3654
                     /locus_tag="H9210_RS25005"
     CDS             3523..3654
                     /locus_tag="H9210_RS25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306295021.1"
                     /translation="MQRAENDPDAFFWFSLDKASLFAVFAAESRSARFCGARNETLW"
                     /name="hypothetical protein"
     gene            complement(4175..4720)
                     /locus_tag="H9210_RS25010"
                     /old_locus_tag="H9210_24810"
     CDS             complement(4175..4720)
                     /locus_tag="H9210_RS25010"
                     /old_locus_tag="H9210_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021543811.1"
                     /translation="MASYFPTLEISGKEFDFTHLEPFIMHVNSDMAKKVLKVHVRFTNH
                     CFTKGYEPFYHPEGEPIIYDHSENKRTFCPIRYRLSLALPEIIKRLCDPAEKVYQTSAK
                     RNWTYSITIDDPEGPYHLFFEIRRASRDERHLQDLNVVIESAYPEDRGYGPPSLLGRIG
                     FVGLCGRVFCGKPLATKR"
                     /name="hypothetical protein"
     Typing          1058..1279
                     /name="rep_cluster_2392"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.067"
                     /coverage="87.0"
                     /evalue="6.509999999999999e-94"
     Typing          complement(2646..2901)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.154"
                     /coverage="99.0"
                     /evalue="3.2100000000000013e-72"
ORIGIN
        1 aaatctctta gtttcctcat cctccctgtc catgtggtta cagctcgctg tccgctaaaa
       61 ccagcctcat aagcaaatcc atctttatcc tttatttcca caaatccttc atcaaatact
      121 ctgcaccaaa gagccagata tgtttgagac aatggctggc ccgtacctcc gaggtaatcg
      181 aggatccgac tgatatatgt cagcgttctt ggcactgttg tatacccgtc atgttcatgg
      241 cgattccata ccttgagttg ttcaagctcg tctccccata gctcttctaa caaagttttc
      301 ctttttgcag caagagttct ccttcctgac ttcttcgctc tttctgaacc actatttttt
      361 actgttgacg gtggcgctat tggtagtggc agtggtggca gtggtagctt cgccattata
      421 ttctccaaaa tcatttaaca tgcggttatt ttagttgttt tttcttttaa aacaatgact
      481 tgtatgttgt tttgtgtgca attgtgctgt tgcctatcaa tctatagaca tcttatgaat
      541 atctatttat tacatctcga tgtatgagtt gtaataaata cagcatatgg tgtcctttat
      601 tcaatgagtt atgactggtt tctgtgctcg ctgtactgaa tgtgctgata tgtgctgaat
      661 gtgctcgctg tgctgaatgg ggatgcaagg gggcaaggta ataattttcg acagctattt
      721 aatacgaagc gaaatggctg ctcactgtgc cctgtgtgcg attttttccg gcattagagg
      781 ggggggggat ggccttggca cttgcttgag tgtgtctgag acgacgtgag agtaatgtgt
      841 ggatgctgat tggggaaatt gacattattc tgcgttaagc gttttgagtt ttacggggag
      901 ggggcgtacc agtgagcatg aaataaaaag gttcttcttg agatcctttt tttctgcgcg
      961 taatctcttg ctctgtaaac gaaaaaacca ccctggcagg tggtttttcg aaggttagtt
     1021 aatcctggca gattttctaa ccgtggtaac agtcttgtgc gagacatgtc accaaatctg
     1081 tcctttcagt gtagccggag taaggccatc acttcaagaa ctctcgatac atctctcgca
     1141 catcctgttt accaatggct accaccactg gcgatttttc gtgtctgttc cgggttggac
     1201 tcaagatgat agttaccgaa acaacgtggt agtcgggctg aacggggggt tcttgcatac
     1261 agcccagctt ggagcgaact gtctaaacgg aacgggacgt ggtgatttag gtaaggcttc
     1321 caccacaaca cgtaagccgc atggcgggaa caggagagcg caagagggag ccaccagggg
     1381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     1441 ttctgtgatg ctcgtcaggg gggcggagcc tatggtaaaa cgcccagggg gcgtggcctt
     1501 ctcatgcttt ttggctgccc tttccttacg tccatcatct actctcttac ctgcttctcc
     1561 gtaagccccg tccgctcgcc gcagacaagt gaccgagcgt agcgagcgaa cggtcgagga
     1621 agcggaaaag aaccagagtt aacatggagc actgacgctg tgatgacgaa accacagaaa
     1681 actgcgctta atatggcgaa gtttattaag gggcagaccc tgcttttgct ggaaaaactt
     1741 aatgaactcg agctcgatgc tgaggcggac ctttgcgaaa agctccatga ggatgcggaa
     1801 ctactctgtc gcactctgac tattcgtctg gatggtcatc agggggatct ataatggcca
     1861 gggtatctat tagcgaagcg gcacgccttg cttgtgtcag tcgtccgact atttacaagt
     1921 taattaaatc tggtgagtta agttacacct ctgttgtaaa gcatgggaaa gctgtaaaag
     1981 ttattgatac tacagagctt attcgtgttt ttggttctct ttcttttgac aatgaacgtg
     2041 accatgctgt tgtaaaattt gacgaccgct cgacaggtgt taacagtgat tttttacaga
     2101 atttacagca tcaggtagca cttttgcagg cagaaaacgc cgggctgaaa gatgcagtga
     2161 atgcccggga cgaacatatc aattcacttc gtcaggccat gcagctactg gagcataagc
     2221 agggcactgc tgatccgcag catgtccgag caaagtcctg gtggcagttt tggaaattcc
     2281 gttgagccat taccacgcat cccagccata gcgttcggtc tgccactcag tttggggtaa
     2341 tttggaaaac ggaaattttc ccacattaag agcattgagt tggagtcgat attcctgcat
     2401 tgctcttttg tcgaccgtaa aatcgccgcc gattcttgca aattcaacac tatcaaatgc
     2461 atattcagtc tgagttcaaa actcaaacgt aagagatagg tagttatttt caccttcccc
     2521 tgcgggaagg tgcgcagggt tttttatcct tgccgctacg gttccggcag agccttgagc
     2581 gcaacaggaa accgtctggg gagaccccca ttgctgtagc actgacggtc tacgaccatc
     2641 taccaaccga gtggcttact gttccgtgcg aattggcttt gcaggggatg actcggttcc
     2701 cagcgacatg aaatccccta gttaggtatc ggcgcgcctg acctcctgaa acgctctgta
     2761 agcgttattc gcgccttcca tgccctgacg ggcatctaaa acccgtttca cgcgtctgag
     2821 tgcttttgag cgagtttctg gggctatctg atgactcatt cctgaaagaa caacaacgga
     2881 atcaccggca acatgagaga gccgtagcga gcattaagca cgttcgcact gatgttgtgg
     2941 gggaaactct gaccaaatgg caatacgttg tgtgtgccgg tattacaatt aaacatcctg
     3001 tcctgtttct tcaggtagta tcatgacaca gcatatcaac tgataaataa tccctgtgca
     3061 gtacagccgc tgttgcataa aatttcgctc tgcgtcgatg tttttttaat caaaaattcc
     3121 gcagataccc cggaaaaacg cgagtctgtt ctcatgcctg aaatgcccac accccacgca
     3181 aaactaactt tttgctgatt ttctttttaa atcatgatgt tattttttta aaaatgtatt
     3241 ttctttttca gatttatgca aaagcacgtc gttgacgacc ctatggcgat gctatgacga
     3301 ccctacttag ggtcagtact gacaatcttt ataaaaaaca gttaattaat gtagggtcgt
     3361 ccctaggggc gcgtcgcatc tttttacagg acacccctag cagcgcccct aggggtgtcc
     3421 tgtaaaaaat gcttggaatt cacgaactga aaatgtctga aaatgtgcct catgggcact
     3481 gtatgcaccc aaaccgttcg ccattcactc tcatgcatag ctatgcagcg agctgaaaac
     3541 gatcctgacg catttttctg gttttcactg gataaagcat ctctttttgc ggtgtttgcg
     3601 gcagaatcgc gttcagcgcg tttctgcggt gcgcgtaatg agacgttatg gtaaatactc
     3661 cgaacgctat gagctgtaaa cggtagacct gaacaagctg ggtgtaacca tcagcatgtt
     3721 tagccacagt aagccatacg gaacgattgc taaaacatca aaaacgatag atacagaagt
     3781 ctaatttttt tcaataaaaa caaatgacta tgttttttct gttgtagcaa aatcatgatg
     3841 ggctacaagg ccatatgtat tttataaagt aactcgaatg ctgagttgtt ttataaaata
     3901 catatggcct tgtaaattca tgcggttaac tatggttttt gtactcatgg tgctctatat
     3961 catttgaggc gttgcattga attgatggtg ctgaattgcg taattggttt tgttatattg
     4021 tttacatcgt agcgactgac cggatgagat tgaacgctac ccagcagggc aaaccttgtt
     4081 cctgtgttcg cgcaaggcga acctaagcaa aagctagtgg tgtccggaac gattaccaaa
     4141 gatgaaaaaa aggcaccttt caggtgcttt tttttcatct tttagtcgca agtggtttcc
     4201 cacaaaatac cctaccgcat aagcctacaa aaccaattcg acctagtaat gacggaggcc
     4261 cgtacccccg atcttcagga taggcactct cgataacaac attcaaatct tgcagatgtc
     4321 tctcatcacg tgaggcgcgt cttatttcaa aaaataagtg atatggccct tccggatcat
     4381 caattgtaat ggagtaggtc cagtttctct ttgccgatgt ttgatacact ttttctgctg
     4441 gatcacatag acgtttgata atctcaggta acgccaaaga taatctatat ctaattgggc
     4501 aaaacgttct cttattctcg ctatggtcgt aaattatcgg ttctccttcc ggatgataaa
     4561 aaggctcata accttttgtg aaacaatgat ttgtgaatct cacatgtact tttaatacct
     4621 ttttagccat gtcactattt acgtgcataa tgaatggctc aaggtgcgtg aaatcgaact
     4681 cttttcctga tatttctaac gttgggaaat agctagccat gaattactcc catttagcgc
     4741 caacctcctg catacgtccc accaatgcgt tatacctttc atctttttct ttaccttcgt
     4801 ataactcttt aatgacagtc agcgggttaa gcaaaattac atattgaaac tcacctgatg
     4861 tgcctggttt tgtagttatg aaacct
//
