LOCUS       NZ_CP060872             2100 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC29 plasmid pEC29-6, complete sequence.
ACCESSION   NZ_CP060872
VERSION     NZ_CP060872.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904668
            Assembly: GCF_022558645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558645.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:06:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,039
            CDSs (total)                      :: 4,919
            Genes (coding)                    :: 4,647
            CDSs (with protein)               :: 4,647
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 272
            CDSs (without protein)            :: 272
            Pseudo Genes (ambiguous residues) :: 0 of 272
            Pseudo Genes (frameshifted)       :: 95 of 272
            Pseudo Genes (incomplete)         :: 178 of 272
            Pseudo Genes (internal stop)      :: 55 of 272
            Pseudo Genes (multiple problems)  :: 50 of 272
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060872.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC29"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC29-6"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-11-21"
                     /collected_by="Long Sun"
     gene            complement(join(1871..2100,1..37))
                     /locus_tag="H9211_RS25025"
                     /old_locus_tag="H9211_24780"
     CDS             complement(join(1871..2100,1..37))
                     /locus_tag="H9211_RS25025"
                     /old_locus_tag="H9211_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(41..1006)
                     /locus_tag="H9211_RS25030"
                     /old_locus_tag="H9211_24785"
     CDS             complement(41..1006)
                     /locus_tag="H9211_RS25030"
                     /old_locus_tag="H9211_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(382..613)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.571"
                     /coverage="100.0"
                     /evalue="9.53e-117"
ORIGIN
        1 tcttatttcc attcaggtat tcaatattaa tattcatacg ttaatccgat ttatccttct
       61 caggggcgcg tttgtaacgc cgtttacctg aatcccagac aaacgccgtc cgcttcccgt
      121 catcagtgcc atcccctaca tcgtcggcaa tgacaagatc ctcattggtt tctcgatcca
      181 actggaggac gttttttagc gccccaccgg tcgagataaa acggcgcttg tgaagctgcc
      241 gcgtcagctc aagaaaccac tcaggatcgt ttgccatatc ttccggttta acggagtatt
      301 tcagcgtttc agcaaccgcg ctttgcagtt gctcggcaac gttggccaca acctcacctg
      361 tcttagtttt tactgcccga atatcgatat tcggctcata gttcacccgc aagcaatcgc
      421 gccagagttc tacccaacgt tcgtgcttaa cgtagttctt ccctttaaac caagaaggtt
      481 gcaccatcag cagacagtga aaatgcggat gtgcgctgcc atccttacct cgtgtcacct
      541 ccgtagccct gatccacccc tgaacaggtg atagctcctt tcgcttttcc attcgcttaa
      601 acgccgcatt cattgctgta aggactgttc caagttcacc tatctcgcag ttcctgacag
      661 ttaacgtcag aaacaaccat cgggaagacg ggtaatccac aacgattttc ggtagagcct
      721 gataaaaacg ggcttgccac atgagggttc ttctccactg gcagacaggg caatgacgga
      781 cacggcaaaa ttgcgcacta cgaagccgta ggcgcgtttc tccggtttcc atgatggtag
      841 accaaccaaa acgtaacaaa ccgctacagg aggccatacg gagtgcatag cgctcgaatt
      901 cagcagcagt ctggtagata cccccgacag aatcggtaag cgtgcgctgg gtatcccaaa
      961 ctgcatcacg ggggctgtag tccgaaagga atttatcttc tgacattaag cctgctccga
     1021 tctgggcagg ctgtgatttg atgtggtata ttcatcacgt acctgtgcat tgcgagccag
     1081 tacatgtcac cagagtttgc cgctgaggtg acacattcga aatgttgggg agtgttgacg
     1141 cactccccaa cgccgattaa aaacgcctca attctgctcc attttctttg acctgtcaaa
     1201 acccactcaa agcctcgtca ttactgggct aaaccactat ttcatatccg ccatttatcc
     1261 cttaagtata aatacaagga tcacgcgcga gaattgcgca aaaaataacc acaattttaa
     1321 cccccaaaac aagcaccccc gaacggctta ccccaacctt ttccacacgc ccgatcacca
     1381 aaacagggaa taaaaacaaa accctgtctt acaggtggat ggcctcactt cgtgaaacca
     1441 tccaccccgc ataagttttt taatcggtgg tgtcgtcaat cactaaatcg gaaccccaaa
     1501 ggggaccccc acgatttagc atttgacgag attaactctc ggagatggca tcatgctttt
     1561 aacggacttt aaaaaacggt gaacggatgc ggattttaat ccgtgtctgt gaggcgttga
     1621 gcaacgagca aagcgaggcg gtagaaagag agccagcggc aggctcagga cgataatccg
     1681 gggaaaatgt accactcgca gagtgttaac atttgacatc ggattgtcta tcctgcattg
     1741 gtgtcgagtt ggcaaggctg aggataatgc tgaggataat gctgaggata atgcaggata
     1801 gacagagcac tagagattgc atgcaatctc gtacctatca cctcagcctg ccgctggctc
     1861 ttttccagaa ctaagaatgc gttcttaaaa atccaaactt gataccgttt aaatctattt
     1921 tcacaaaaag agtttttacc ctctcgtttg ttttctgaat atataaaatt aattcatcct
     1981 caaaaccttc gtgcggatga aaaccaaatg tgccaaaact taatccatca ataagcaaaa
     2041 taaggttttt aaacctgtca ggaatcaccg cttcacttct taaaaaaaat aaaccaatag
//
