LOCUS       NZ_CP060870             4071 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC29 plasmid pEC29-4, complete sequence.
ACCESSION   NZ_CP060870
VERSION     NZ_CP060870.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15904668
            Assembly: GCF_022558645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ruan,Z.
  TITLE     Complete genome sequence of carbapenem resistant Escherichia coli
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022558645.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:06:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,039
            CDSs (total)                      :: 4,919
            Genes (coding)                    :: 4,647
            CDSs (with protein)               :: 4,647
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 272
            CDSs (without protein)            :: 272
            Pseudo Genes (ambiguous residues) :: 0 of 272
            Pseudo Genes (frameshifted)       :: 95 of 272
            Pseudo Genes (incomplete)         :: 178 of 272
            Pseudo Genes (internal stop)      :: 55 of 272
            Pseudo Genes (multiple problems)  :: 50 of 272
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060870.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC29"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC29-4"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-11-21"
                     /collected_by="Long Sun"
     gene            join(3945..4071,1..575)
                     /locus_tag="H9211_RS24980"
                     /old_locus_tag="H9211_24740"
                     /pseudo=""
     CDS             join(3945..4071,1..575)
                     /locus_tag="H9211_RS24980"
                     /old_locus_tag="H9211_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(832..2979)
                     /gene="mobQ"
                     /locus_tag="H9211_RS24985"
                     /old_locus_tag="H9211_24745"
     CDS             complement(832..2979)
                     /gene="mobQ"
                     /locus_tag="H9211_RS24985"
                     /old_locus_tag="H9211_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_021526521.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3158..3412
                     /gene="mobC"
                     /locus_tag="H9211_RS24990"
                     /old_locus_tag="H9211_24750"
     CDS             3158..3412
                     /gene="mobC"
                     /locus_tag="H9211_RS24990"
                     /old_locus_tag="H9211_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2622..2980)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2981..3157
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..575
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(836..3066)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggatgaaca aaaaaccatg accatcacgg ctgcagagtt tcatgatgcc tttcctgaca
       61 tgggacggca gcacgctgaa gctaggctca aggaagcgat agatcgcctc tgggacaggt
      121 cagtaatcat caagaacgag gaaaaacgag aagagttccg ctgggtgcag taccgggcgc
      181 agtatttcaa aggtgaggcg aaagtagata tcaccttctc cgatgccatc atgccgtatc
      241 tgacacagct caaggggcag tttacgcggg tagtcgtgaa gaacgtctcc agcctttcca
      301 gcacctacag catccgcatt tacgagctgc tccagcagtt ccgcagcacc ggtgacagaa
      361 cgatagctat cgatgatttc cgaaccatgc ttgccgttga agataaatat caacaattca
      421 aagacttgaa taagatgata atcaagccag ccatcgccga actgaacgaa aaaagcgatc
      481 tggttgtaac tgttgagacg atcaaaaagg ggcgcacggt tgtagccctg cattttcgct
      541 tcaaggaaga caaacagatc aagatgacga tctaaaatcg attttaaggc acctaaaacg
      601 ctccctgctg cgtttttttt gtgttagtag tgcgaaggta ctattgaagt gaaaaattgc
      661 gtcaaaaacg ctctgatgca gcgaaaaaag acccccaatt tttgggggcg gaaaaacaaa
      721 actatgcttg cgttatgtga gtgtgcttct tgcattcatc cctgtcgatg aacatcgaag
      781 aacatcgacg aacataacac gatgttaaac aaaacaaaat attgttcaca ttcactgtga
      841 ccaaccgcga tcatgtctcc tctcggtggt gtgtgttcgt gcatcttcct cgcgctgcca
      901 ttcctcccag gtacgattaa aacccagcga gcggaacaga tgatccggct gtttttctgg
      961 tgtgttgacg gcagcaaaaa gcggtgcctc acgggttttc agatgaaact gcatcagttc
     1021 tggtgcaggt gatatgtggc tgtctgcatc gtgatttacg gcaacaccat accctgcata
     1081 ccgtaacccc ttatcggtca gcgcactgat gaccagatgc ctgttatcgg ctgagggaac
     1141 ggcaaacgcg ggatacagcg taccattaag ccggataaca ccgcccagag agtcatccac
     1201 gcccatgacc agcaaaagct gaagctgacg gttttcccgg tcgacagaac tcatgaccgg
     1261 catcagcgtc ggtggcagac gtccctgcat aaaatccacc ttccttgccg ctggtgatgg
     1321 catggctggc tggcggtctt ctccaagaca gaaagactcc ggccatgtct tcggctggcg
     1381 gagattgcgg gtatgttgcg gccatgtctg gcaaacctgc gtcagcagtt gctcaaaggt
     1441 tgcagaggaa taaacacgtt gcagtggcgg acgttggccg atttcgcttc ggtcacagat
     1501 ataaccactc ctgtcacgca ttaaatcaac actctcaaag attctggtgc ggtatttctc
     1561 cagctcgttc ccccagagcg tatattctcc gttccggttg tgaagcagaa ggtaacaggt
     1621 catttccccg ttaccaggca tgggagcctc gccatacccc gtaacgactg ctctcagcac
     1681 ccctgaatcc ataacggcat tgtcattttt cctgagctgc tccgcgcgac tacgttcagt
     1741 atcttcctgt tccttccggc gcaattcctc ttcacgttta agcaactggg ctctgagagc
     1801 tgcaaggccg gattcccccc actgctgcca ctgtttttca aatgccatct gactcgcggg
     1861 aatgtcctgt ttctccacct tccggtaatg ctgtcgtaat ttttccattt gctggctcag
     1921 agactggatt tttccctgct cactacggta ttccggtgcc gcccattgca tcaggggaat
     1981 acgggaaaaa ccggacgaaa acggcgatga ttttttcagg ctggcagcat ggctcatcgc
     2041 ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc gtcgcctgca tatctgccat
     2101 catcgacatg gcattactgg tgagtttcca tgccgagtta ctgtccttcg gcaacgcttc
     2161 gataatgtca cgtagtgtgt acgaagttgc gttgtcagga agcccgatat tcagaaggtc
     2221 agccacacgg cgggcaggct ctgtcagccc tccgttcctg agttcggtgc gggaaatctg
     2281 tatttccagg ttaagcctgt taatcagacg attctgttcg ccgcgttctg tttcgatacc
     2341 gcgtgtttcc atcgcacagg cagactttcc gaggtgaata ccaggcgttt tctcaagccc
     2401 ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa ccggcgttcg ccagtgccct
     2461 gtttgcatgg tcagcccatg atgcccgcca cgtctcggca tgtgtccggt cgttccagtc
     2521 cctgaccttt ccgctgaacc cagcaggtcc gacagccctc gtggtcagca tgatgtgcgc
     2581 atggggattt gttttatcca tgtgatggaa cgccacatca gcaatcatgc cctgactgac
     2641 aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg gcatcctgcg gcagttcgtg
     2701 ggggatggcg agttcaattt cccgcgcaag ttgtgagttc ttccggcgtt cggccttctc
     2761 aacggcattc cagagttcgg cacggttcgt acaccaggca ggggcggtgg cgggggtcag
     2821 aatcacagcc tcagccacac cgcttttgcg ggtgtagtcg tgagtcagtc cggtacgctc
     2881 atcaagcatc agtgaaccgg aacgataggc agcggaagct acggcgctgt agccgctgct
     2941 tctggagata attttcatgc tgagatgata aatcgccatt tctgccgtaa tgcctttttg
     3001 tagttttctt tcagccccgc agggcgcaca caatcgaaac ggagtggaga ttgtataagt
     3061 gcgcacttcg tgatttgccg tgcattatgc cagctcagtt tttaaactac cagtgacgcg
     3121 ctaaaatgcg atttttcatc gcgcacggag ttacatcatg agcagaacgc tcgaacagaa
     3181 gattgctgat gctgaggcga gattacagcg cctgaaggcg aagagccgga gcctggacac
     3241 agcgcagaag gttgttgtgg gtgctgcatt gctggcaaag gtcagaaagc cggaggaggt
     3301 gcagttgcgt gcctggttac tccagttcct gaaggcagag gtgacacgac aggctgatgt
     3361 gacgcgcata ctgccgctga ttaacgagct ggaggcgctg ccagaacagt gattgtatcg
     3421 tccgctcgcc gcagccccag cgaccgagcg ttagcgagcg agtgggcgag gaagcggaag
     3481 tgaatcagtc gccaggttga gcacatacgc acggtttttc ggcaggtgat atgcgctgaa
     3541 cccgcatgaa tactgggttt ccacagggtt ggagggggtg tattcgaaac ttgcaattct
     3601 gctttattgc aacgcattgt ttttattggt tttttgtttt cagcacccca agttatccac
     3661 aggctcgccg ttaagttttt aagatcttat ttttagatct tttttttaaa acctttttag
     3721 gtcttgtaac accatgattt taaagatgtt ctggcaagat aagtggagct tttgggggtg
     3781 aaaggtggag cttttggggg tgaaaggtgg agcttttggg ggtgaaaggt ggagcttttg
     3841 ggggtgtaat ccacagaaaa agggggttgc aaaaaaaaaa cttccaccca tatgataagt
     3901 ggcgtacagt aaaaaaacgc caccaatagc aacgagcaag cgaaatgtca gatctgatag
     3961 cttacaaatc aaacgccctt atcgaggcaa gctacaagct cactttgcag gaacagcgct
     4021 tcctgctggt ctgtatcagc cgcctgaaat ccggagaaga ttccccttcg c
//
