LOCUS       NZ_CP060747             2308 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP120 plasmid pKP120-2Kb, complete
            sequence.
ACCESSION   NZ_CP060747
VERSION     NZ_CP060747.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15903654
            Assembly: GCF_014361115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Yin,M., Hu,G., Shen,Z., Fang,C., Zhang,X., Li,D., Doi,Y., Zhang,Y.,
            Wang,M. and Guo,Q.
  TITLE     In vivo evolution of CTX-M-215, a novel narrow-spectrum
            beta-lactamase in an Escherichia coli clinical isolate conferring
            resistance to mecillinam
  JOURNAL   Antimicrob. Agents Chemother. (2020) In press
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Guo,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2020) Institute of Antibiotics, Huashan Hospital,
            Fudan University, 12 M. Wulumuqi Road, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.5.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 690x
            Sequencing Technology             :: Illumina, PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:16:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,484
            CDSs (total)                      :: 5,366
            Genes (coding)                    :: 5,228
            CDSs (with protein)               :: 5,228
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 58 of 138
            Pseudo Genes (incomplete)         :: 70 of 138
            Pseudo Genes (internal stop)      :: 25 of 138
            Pseudo Genes (multiple problems)  :: 15 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060747.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP120"
                     /isolation_source="bile"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP120-2Kb"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.22 N 121.46 E"
                     /collection_date="2011"
                     /collected_by="Institute of Antibiotics Huashan Hospital,
                     Fudan University"
     gene            960..1433
                     /locus_tag="IAI03_RS27225"
                     /old_locus_tag="IAI03_27225"
     CDS             960..1433
                     /locus_tag="IAI03_RS27225"
                     /old_locus_tag="IAI03_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228728757.1"
                     /translation="MRFDSKAAKKKLRVNVRFSNHCFSHSPVDGVSYSNEFKLHDHNGK
                     DRIFCPIRFRLSQDLPKIVLSLNNPQCKVYQTAARRNFNYSLEIEDPRGPYHLFFEVSK
                     ATGKAAGMQDLSLFLESAYPEEAERGKPDLLGRIGFHTLCTNVFLRKPVATKR"
                     /name="hypothetical protein"
     gene            complement(1909..2145)
                     /locus_tag="IAI03_RS27230"
                     /old_locus_tag="IAI03_27230"
     CDS             complement(1909..2145)
                     /locus_tag="IAI03_RS27230"
                     /old_locus_tag="IAI03_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(2..176)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.079"
                     /coverage="82.0"
                     /evalue="5.83e-49"
     Typing          152..638
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.627"
                     /coverage="93.0"
                     /evalue="6e-157"
ORIGIN
        1 cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctt
       61 aatatgttgg atcggagtgg taattcggaa aagtgattca tgtgacaggg gaaaatgcag
      121 ctgcgaggcg taagtgcgaa atgcaggtaa cggtgaaatg acgcctactc gctcactagg
      181 tcgctacgct cctgccgtga gactgcgtcg ggcgttacct gctcacgaat ggcgagagaa
      241 agaggctgag cggaaatcag gaagggctgg gaaaggatgc ggcgttgccg tttttccata
      301 ggcttcgccc ccctgacgag tatcacgaaa tctgacactc aaatcagtgg tggcgaaacc
      361 cgacaggact ataaagatcc caggcgtttc cccctggtag ctccctcgtg cgctctcctg
      421 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
      481 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
      541 cccgttcagt ccgactacca cgcccattcc ggtaactatc aacttgagtc caacccggaa
      601 aggcacgaca aatcgccatt ggcggtagcc actggtaatc aggaattagt ggatttagat
      661 atcgagagtc ttgaagtggc ggcctgactg cggctacact gaaaggacag tatttggtat
      721 ctgcgctcca ctaaagccag ttaccttggt taaaagatct gccaggattt tttaaccttt
      781 gaaaaagcca ccttccaggg tggttttttc ttttaaacga tagtacttat accaacccca
      841 gaactcaata ttacttttat aaatcattgg gggttcaatt gacgaacgca gcacctttct
      901 tcccctcact cattattgcg gatacgactt atgagttctt acatctggaa ccattcacta
      961 tgcgtttcga cagtaaagcc gccaaaaaga agctaagggt gaacgttaga tttagtaatc
     1021 actgtttttc tcattcgcct gtcgatgggg tttcgtattc aaatgaattt aaactgcatg
     1081 atcataatgg aaaagatcgt atcttctgcc ccatcagatt taggctgtcg caggatctac
     1141 caaaaatagt gttaagtctc aataacccac agtgcaaagt ttaccagaca gcagcaaggc
     1201 gcaactttaa ttacagcctt gagatagaag atccgagagg tccatatcac ctgttttttg
     1261 aggtttcaaa agcaactggt aaagcggcag ggatgcagga tttaagctta tttctggaaa
     1321 gcgcttaccc tgaagaagca gaacgaggaa agccagacct tttaggccgc ataggattcc
     1381 atacgttgtg tactaacgtt tttttgagaa aaccggtagc cacaaagcgt taaaacgaaa
     1441 atgggcacct gtaaaggtgc ccatctcgaa tctcgatggt atcggccaaa ccgataccct
     1501 atccggccac cactttcgac ccgccttgcg caggtacagt tttcactgct ggggaacgct
     1561 atctcatccg gtcattcgct ccgacgtaaa ataattacgt ctggcgtaat catatatcga
     1621 tgttgacggc ttcgtcaaat ttgagcaagc aagctttgcc ttactctgaa aaattaacgc
     1681 caatgagtaa ccaaaaacaa acagaattga acataacatc tcttgggcgc actgcctgag
     1741 aggccctcag cgcgtttctg cggcctgaca gccgtttccg gcacctttca ccgcaccaac
     1801 ccctgaaaaa cgcccctgat gatttctgcg cgtctgcata gctattcaca ggagtgaatg
     1861 gcgggcaatt tcggagtgtc agcaccacta aaccgccaaa aggcgcaatc agcggcgcgg
     1921 cgcgcgcgga cgtgaccagg caaactcgga ctgctgccgc tcatcctcac gtaatgcacg
     1981 ctggcggtcg gcgtaacgct gcgactcaat ctgcgcacgc gctctgttca ggacgatatg
     2041 ccggttcggc tgatttagct ttttgcctga cgctctctca gctgcggcaa tagcatcatc
     2101 aagaaatttt ttctctgcag gtgtcaggtt tgtcagtcta ttcatagcat tctcccatca
     2161 gaataacgcc gcacagtctg ccagacacag cagaaccgat gaagcgctgg cggtgctgaa
     2221 agacagccag aaacggtgaa cgggagcgga ttttaatccg gtgacgtgag acgttgggca
     2281 agccgcaggc gcgtcagtgg ctttttag
//
