LOCUS       NZ_CP060427             2089 bp    DNA     linear   CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA2105 plasmid pColBS512.
ACCESSION   NZ_CP060427
VERSION     NZ_CP060427.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402336
            Assembly: GCF_011742505.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBA2105 hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
REFERENCE   3  (bases 1 to 2089)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:27:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,872
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,563
            CDSs (with protein)               :: 5,563
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 71 of 182
            Pseudo Genes (incomplete)         :: 100 of 182
            Pseudo Genes (internal stop)      :: 36 of 182
            Pseudo Genes (multiple problems)  :: 23 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060427.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA2105"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColBS512"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            77..343
                     /locus_tag="HBJ34_RS29120"
                     /old_locus_tag="HBJ34_029115"
     CDS             77..343
                     /locus_tag="HBJ34_RS29120"
                     /old_locus_tag="HBJ34_029115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            1197..>2089
                     /locus_tag="HBJ34_RS29125"
                     /old_locus_tag="HBJ34_029120"
     CDS             1197..>2089
                     /locus_tag="HBJ34_RS29125"
                     /old_locus_tag="HBJ34_029120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_114500989.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDG"
                     /name="protein rep"
     Typing          1591..1822
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gcggacggcg tttgtctggg attcaggtaa acggcgttac aaacgcgccc ctgagaagga
       61 taaatcggat taacgtatga atattaatat tgaatacctg aatggaaata agactattgg
      121 tttatttttt ttaagaagtg aagcggtgat tcctgacagg tttaaaaacc ttattttgct
      181 tattgatgga ttaagttttg gcacatttgg ttttcatccg cacgaaggtt ttgaggatga
      241 attaatttta tatattcaga aaacaaacga gagggtaaaa actctttttg tgaaaataga
      301 tttaaacggt atcaagtttg gatttttaag aacgcattct tagttctgga aaagagccag
      361 cggcaggctg aggtgatagg tacgagattg catgcaatct ctagtgctct gtctatcctg
      421 cattatcctc agcattatcc tcagccttgc caactcgaca ccaatgcagg atagacaatc
      481 cgatgtcaaa tgttaacact ctgcgagtgg tacattttcc ccggattatc gtcctgagcc
      541 tgccgctggc tctctttcta ccgcctcgct ttgctcgttg ctcaacgcct cacagacacg
      601 gattaaaatc cgcatccgtt caccgttttt taaagtccgt taaaagcatg atgccatctc
      661 cgagagttaa tctcgtcaaa tgctaaatcg tgggggtccc ctttggggtt ccgatttagt
      721 gattgacgac accaccgatt aaaaaactta tgcggggtgg atggtttcac gaagtgaggc
      781 catccacctg taagacaggg ttttgttttt attccctgtt ttggtgatcg ggtgtgtgga
      841 aaaggttggg gtaagccgtt cgggggtgct tgttttgggg ggttaaaatt gtggttattt
      901 tttgcgcaat tctcgcgcgt gatccttgta tttatactta agggataaat ggcgggtatg
      961 aaatagtggt ttagcccagt aatgacgagg ctttgagtgg gttttgacag gtcaaagaaa
     1021 atggagcaga attgaggcgt ttttaatcgg cgttggggag tgcgtcaaca ctccccaaca
     1081 tttcgaatgt gtcacctcag cggcaaactc tggtgacatg tactggctcg caatgcacag
     1141 gtacgtgatg aatataccac atcaaatcac agcctgccca gatcggagca ggcttaatgt
     1201 cagaagataa attcctttcg gactacagcc cccgtgatgc agtttgggat acccagcgca
     1261 cgcttaccga ttctgtcggg ggtatctacc agactgctgc tgaattcgag cgctatgcac
     1321 tccgtatggc ctcctgtagc ggtttgttac gttttggttg gtctaccatc atggaaaccg
     1381 gagaaacgcg cctacggctt cgtagtgcgc aattttgccg tgtccgtcat tgccctgtct
     1441 gccagtggag aagaaccctc atgtggcaag cccgttttta tcaggctcta ccgaaaatcg
     1501 ttgtggatta cccgtcttcc cgatggttgt ttctgacgtt aactgtcagg aactgcgaga
     1561 taggtgaact tggaacagtc cttacagcaa tgaatgcggc gtttaagcga atggaaaagc
     1621 gaaaggagct atcacctgtt caggggtgga tcagggctac ggaggtgacg cgaggtaagg
     1681 atggcagcgc acatccgcat tttcactgtc tgctgatggt gcaaccttct tggtttaaag
     1741 ggaagaacta cgttaagcac gaacgttggg tagaactctg gcgcgattgc ttgcgggtga
     1801 actatgagcc gaatatcgat attcgggcag taaaaactaa gacaggtgag gttgtggcca
     1861 acgttgccga gcaactgcaa agcgcggttg ctgaaacgct gaaatactcc gttaaaccgg
     1921 aagatatggc aaacgatcct gagtggtttc ttgagctgac gcggcagctt cacaagcgcc
     1981 gttttatctc gaccggtggg gcgctaaaaa acgtcctcca gttggatcga gaaaccaatg
     2041 aggatcttgt cattgccgac gatgtagggg atggcactga tgacgggaa
//
