LOCUS       NZ_CP060426             6047 bp    DNA     linear   CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA2105 plasmid p_unnamed_1_VBA2105.
ACCESSION   NZ_CP060426
VERSION     NZ_CP060426.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14402336
            Assembly: GCF_011742505.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae VBA2105 hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
REFERENCE   3  (bases 1 to 6047)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:27:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,872
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,563
            CDSs (with protein)               :: 5,563
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 71 of 182
            Pseudo Genes (incomplete)         :: 100 of 182
            Pseudo Genes (internal stop)      :: 36 of 182
            Pseudo Genes (multiple problems)  :: 23 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060426.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA2105"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p_unnamed_1_VBA2105"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            <1..344
                     /locus_tag="HBJ34_RS29080"
                     /old_locus_tag="HBJ34_029075"
                     /pseudo=""
     CDS             <1..344
                     /locus_tag="HBJ34_RS29080"
                     /old_locus_tag="HBJ34_029075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(<580..822)
                     /gene="glmM"
                     /locus_tag="HBJ34_RS29090"
                     /old_locus_tag="HBJ34_029085"
                     /pseudo=""
     CDS             complement(<580..822)
                     /gene="glmM"
                     /locus_tag="HBJ34_RS29090"
                     /old_locus_tag="HBJ34_029085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(909..1724)
                     /gene="sul2"
                     /locus_tag="HBJ34_RS29095"
                     /old_locus_tag="HBJ34_029090"
     ARG             complement(913..1724)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2184..2342)
                     /locus_tag="HBJ34_RS29715"
     CDS             complement(2184..2342)
                     /locus_tag="HBJ34_RS29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2913..3062
                     /locus_tag="HBJ34_RS29100"
                     /old_locus_tag="HBJ34_029095"
     CDS             2913..3062
                     /locus_tag="HBJ34_RS29100"
                     /old_locus_tag="HBJ34_029095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(3807..4247)
                     /locus_tag="HBJ34_RS29105"
                     /old_locus_tag="HBJ34_029100"
     CDS             complement(3807..4247)
                     /locus_tag="HBJ34_RS29105"
                     /old_locus_tag="HBJ34_029100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            4465..4854
                     /locus_tag="HBJ34_RS29110"
                     /old_locus_tag="HBJ34_029105"
     CDS             4465..4854
                     /locus_tag="HBJ34_RS29110"
                     /old_locus_tag="HBJ34_029105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4898..>6047
                     /locus_tag="HBJ34_RS29115"
                     /old_locus_tag="HBJ34_029110"
     CDS             4898..>6047
                     /locus_tag="HBJ34_RS29115"
                     /old_locus_tag="HBJ34_029110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_193328776.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLT"
                     /name="IS91-like element ISVsa3 family transposase"
     Typing          2705..2878
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(2176..2781)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcttccacgg ggtgttcgca cccaacagtc ggcaccgggc gttggtcacg ccggcaaaac
       61 ggggcagggg caacaaggtc agggtggctg atgaaccggc aacaccagca caacggcgag
      121 cgtcgatgac atgggcgcaa cggctcaagc gtgttttcaa tatcgacatc gagacctgca
      181 gcggctgcgg cggcgccatg aaagtcatcg cctgcattga agaccctata gtgatcaagc
      241 agatccttga tcacctgaag cacaaagccg aaaccagcgg gaccagggcg ttacccgaaa
      301 gccgggcgcc accggctgag ctgctcctgg gtctgtttga ctgacgagcc tgaaggccaa
      361 cgataccaat caaaatgctg cgttcacagc gccgcggcag ggatccgccg tgctggttgt
      421 cggaaaagga gccgctagtg ggaaagagga gggtaaattt tcagcgttgc tggctccccg
      481 tcagccggat tgggttgcat cgcaggggtg tcgaaagagt caactgcggt ccaaagctgt
      541 tggacttggg tgaaaagggc gtttattctt cctatacgtt gtcggcagcg ggccaaaaag
      601 gaatacgtcc atgcccatcg aggtgaaacc ggctgtgagc gcgggttcaa gcatatagcc
      661 cgacaggcgc gtatccttgc cgatcacgac acgatggcgg tggtcaccgc gacgaaagac
      721 acggccagcc gccatgccga cgcgcaaggc ggtttccgcc gtcatcgcgc cttcgttggc
      781 tttgccacga ataccgtctg tgccgaaata tttgcgcacc ataaggtcga ttatcctgtc
      841 gtcgggtcgc cctcaaaggg gacatgcctg ctgaaccgcg aatatagaga aatatcccga
      901 atgtgcagtt aacgaattct tgcggtttct ttcagcgccg ccaataccgc cagcccgtcg
      961 cgcaaggggc gcggctcgtg tgtgcggatg aagtcagctc cacctgcggc ggcggcaagc
     1021 tctgcagcga gtgtcgcggc cccgacatcc cccggaccac ggcctgtgag cgcgcgcaga
     1081 aaggatttgc gcgaaacaga cagaagcacc ggcaaatcga agcgcagccg caattcatcg
     1141 aaccgcgcca gcaccgagag cgaggtttcg ggagcagccc ccagaaaaaa ccccatgccg
     1201 ggatcaagga caaggcggtt gcgtttgata ccggcacccg tcagcgccgc gatgcgcgcg
     1261 tcaaagaacg ccgcaatgtg atccatgatg tcgccagcgg gtgcctcgcg ccgatctgcc
     1321 tgcccgtctt gcaccgaatg cataacgacg agtttggcag atgatttcgc caattgcgga
     1381 tagaacgcag cgtctggaaa accgcgaata tcattgagat aggccacacc acgcgacaag
     1441 gcataggctt gcgtcgcggg ttgataactg tcgagcgaga cgggaatgcc atctgccttg
     1501 agcgcgtcca gcaccggcgc gatacgcgcg atttctgtgt cggacgaaac aggcgcggcg
     1561 tcgggattgc tggatgccgg accgaggtcg atcacatctg ccccctcggc catcagctta
     1621 cgcgcctgcg caatggctgc gtctggcgcc agataccggc ctccatcgga gaaactgtcc
     1681 gaggttatgt tgacgatgcc gaaaatgatg agcgatttat tcatgggggc ttctataata
     1741 ataataatcg agcatgagtc tcatacggat gctcgggtcg aaagggaatc cccaggcgag
     1801 taacctgttt gcggtgatcc gtttcatgat gatgtatctg tagcattttg gaaataaata
     1861 tatcatctct caataagtag atatatttat cttttttgct tctttggtgc tgcaagtcac
     1921 caatacgagg gaaacctgtt acggagcggt ttaaccgtaa tcaacagtga ctggtgtcat
     1981 gttcggaaga ttaattgata gtaatctaca aaggatcttc ttgagatcct tttcaatgaa
     2041 tcgtaatctc ttgctctgta aacgaaaaaa ccgcctgggg aggcggtttg ttcgaaggtt
     2101 aagtcagttg gggaactgct taacccggta acagggttca cgagaccgcc attaccaaaa
     2161 ctgttctttc agtgtagccg cagtcaggcc tctacttcaa gacctcttaa tctatcttct
     2221 cgtgcactcc tgttactaat ggctgctgcc agtggcgttt tgtcgtgtct ttccgggttg
     2281 gactcaagtt gatagttacc ggacaaggcg cagcggtcgg actgaacggg gggttcgtgc
     2341 atacagtcca gcttggagcg aactgccttc acggaactga gtgtcaggcg tggaatgaga
     2401 aaccgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     2461 acgagggagc taccaggggg aaacgcctgg gatctttata gtcctgtcgg gtttcgccac
     2521 cactgatttg agcgtcagat tttgcgatgc ttgtcagggg ggcggagcct atggaaaaac
     2581 ggcttcgccg tggccttatc tcttctttgc taagtgtctt cctggtatct tccaggaaat
     2641 ttccgtcccc gtccgtaagc cggtaccgct cgccgcagcc gaacgaccga gcgtagcgag
     2701 tcagtgagcg aggaagcgga atatctcctg tatcgcatat tctgctgacg cgccggtgct
     2761 gccttttttc tcctgccaca tgaagcactt cactgatttt cgcaatcgtg gcaacatagt
     2821 aagccagtat acactccgct agcgctacat gactggttca gggctgcgcc ccgaaacccg
     2881 ctaacaacct ctcagctact ggatatcacc ccatgccatc tgaatatgat gaacaactgg
     2941 attcgactga tacgcagctt cccggcgaat atgacctttt tgatttaatg gcgcaggtta
     3001 ctgaagagaa cctgcatgcc cctgtggatt ttgggtcgcc tgtcggccgg gaagcgttat
     3061 agctttttcg cttgcccgcg tacgtggtaa tactccggta ttacctttta tttctttatt
     3121 ttcatgtaat tacagtggat ttttaatacc cgggtattac tcgtgaaaac gaaatattcc
     3181 ttttttttct tttaattcat gcagttgcga tgaaaatcgc ggctgcgtta ggtgtatgac
     3241 cattttgagg ttgaaaaata atcaaccatg gcgggtgatt tacgcaggtt aaggggagat
     3301 cgtgacaaaa aaagcgttcc tcactgtatc gttcatgcct gcatgaacgg gttattcttc
     3361 ctgactacag aattacatcg ctccttcagt tcatacatca cttttacatc acttatacat
     3421 cacttttaca tcattccttc agctcatacg tcactttcat cctctgtgcc atttgtacat
     3481 cacccccttc ctgaacgtgc tgacagcggg ttttccggct gcgccggcaa cgcttcacgg
     3541 ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg
     3601 acttaccccg gctgaatggt gtgaatttcc gcgctatgca cttgtgcgca tactcatgca
     3661 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     3721 taccggtttc ccgtcagaaa cgccctgagg gcctctcagg cggtgctgat aacagatgtt
     3781 atggtaaatc agagtcacct gcactgttac tctgaaccgg agcgaaacag ccgcgaccag
     3841 aatccccgct ttttttcctg tgccagcgcg tcagccagac ggctaatttc ggcctgtagt
     3901 tgttccctct cctcatttcg caggcggttt tctgcttccc gcgccatctt gtcttcaagc
     3961 attagggtaa cagtctgctg cagagcgtcc agtttctgct gaatgacgtc tagctgagca
     4021 ttttccgcct ggctaacgac agatttggag tgtccctcct tgtgcctttt tggtgtctca
     4081 gaagtgcgca gctcgccata ggcccgaatc agttcactgg tctcgaactc ccggcgatca
     4141 ttttttcctg tgcgatagct gacaaggcct ttagccatgt cacgatataa ttgcgagcgc
     4201 gactttcctg ttaatttcag tgtctcttga actgtgtgcc atgccataaa agtgacagtg
     4261 tctcctgtgt gtaccgtatt gtaacagcag aacatattac tgttttaaca caaaaggtct
     4321 gaggctgccc gtgaggcgtg aaaaatacac aaaaaacctg ttgacggtgt ggttttgacg
     4381 ggcgtagtat ttcgaatact gtatataaac acattgttat aaatacagtt tttgctgttt
     4441 ttaaaagtca aagaggataa ttttatgagt acagagttaa atagcggtac tgtcggtggt
     4501 tttggtcaca tgggaccaaa tgacccctac aatgatggtc atagcggctc tggaagttcg
     4561 ggaggtagtt ttaacggtgg tggtaatggt ggccatgata atagtagtgg ttcatcttat
     4621 actgcatcct ttggtctcat cactggccga cacccgataa ctgtcccagg tacgaagaat
     4681 attaattctg tcgatctttc tgctgtcatt aataacatta tacatcatga aggtgcacgc
     4741 aataacatgt acctagatac tgcgaacaat gtaagagtgg gagtgacggg cactggctgg
     4801 caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg ctaagcgata
     4861 gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg cggccaggac
     4921 ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc aaacccttct
     4981 ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag agcagggaaa
     5041 ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg ggcggctgga
     5101 gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg tcgctttcag
     5161 ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg cggatggccg aaagtgccgc
     5221 cttgctggtt gatgaagtac tgcctgaaca acccatgcgt cagtgggtgt tgagcttccc
     5281 gtttcagctg cgtttcctgt ttgccagccg gcccgagatc atggggtggg tgctgggcat
     5341 cgtttaccgc gtcattgcca cgcacctggt caagaaagcg ggccataccc accaagtggc
     5401 caagacgggc gcggtcaccc tgatccagcg ttttggatcg gcgctcaatc tgaatgttca
     5461 cttccacatg ctgtttctcg acggtgtgta tgtcgagcaa tcccacggct cagcgcgttt
     5521 ccgctgggtc aaggcgccga ccagcccaga gctcacccag ctgacgcaca ccatcgccca
     5581 ccgggtgggt cgctatctgg aacggcaagg cctgctggaa cgggatgtcg aaaacagcta
     5641 tctggcctcg gatgcggtgg atgacgaccc gatgacaccc ctgctggggc actcgatcac
     5701 ttaccgtatc gctgtcggtt cacaggcggg gcgaaaggtg ttcactttgc aaactctgcc
     5761 gaccagtggt gatccgttcg gtgacgggat tggcaaggta gccgggtcca gcctgcacgc
     5821 cggcgtggcg gccagggccg atgaacgcaa gaagctcgaa cggctgtgcc ggtacatcag
     5881 ccgcccggcg gtatccgaga agcggctgtc gttaacacga ggcggcaacg tgcgctacca
     5941 gctcaagacg ccgtaccggg acggcaccac gcacgtcatt ttcgaaccat tggatttcat
     6001 tgcaaggctg gccgccctgg taccgaagcc cagagtcaac ctaaccc
//
