LOCUS       NZ_CP060118             7231 bp    DNA     circular CON 16-APR-2024
DEFINITION  Shigella sonnei strain UKMCC_1015 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP060118
VERSION     NZ_CP060118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15437744
            Assembly: GCF_014217935.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Wong,B.C., Md Zoqratt,M.Z.H. and Tan,H.S.
  TITLE     Complete genome of Shigella sonnei UKMCC1015
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Wong,B.C., Md Zoqratt,M.Z.H. and Tan,H.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2020) School of Science, Monash University
            Malaysia, Jalan Lagoon Selatan, Subang Jaya, Selangor 47500,
            Malaysia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-AUG-2020
            Assembly Method                   :: Flye v. v 2.6; Pilon v. v 1.23
            Assembly Name                     :: UKMCC_1015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 49.0x
            Sequencing Technology             :: Oxford Nanopore; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014217935.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 07:25:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,006
            CDSs (total)                      :: 4,870
            Genes (coding)                    :: 4,287
            CDSs (with protein)               :: 4,287
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 583
            CDSs (without protein)            :: 583
            Pseudo Genes (ambiguous residues) :: 0 of 583
            Pseudo Genes (frameshifted)       :: 173 of 583
            Pseudo Genes (incomplete)         :: 396 of 583
            Pseudo Genes (internal stop)      :: 86 of 583
            Pseudo Genes (multiple problems)  :: 71 of 583
            Pseudo Genes (short protein)      :: 3 of 583
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060118.1.
            Bacteria and source DNA available from School of Science, Monash
            University Malaysia.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="UKMCC_1015"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="unnamed1"
                     /geo_loc_name="Malaysia"
                     /lat_lon="2.9305 N 101.7787 E"
                     /collection_date="2018-06"
                     /collected_by="UKM culture collection"
     gene            complement(60..374)
                     /locus_tag="HYI41_RS24885"
                     /old_locus_tag="HYI41_24655"
     CDS             complement(60..374)
                     /locus_tag="HYI41_RS24885"
                     /old_locus_tag="HYI41_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024139128.1"
                     /translation="MSNNYDLIAKAIKEKLQVTAYYQGFYREMCPHALGSKKGRKQALF
                     YQFGGESSKGTVTPGSTFNWRCIPIDGLTEVTLQSGQWYTAENHSQAQTCVDQIDIEVQ
                     "
                     /name="hypothetical protein"
     gene            complement(466..768)
                     /locus_tag="HYI41_RS24890"
                     /old_locus_tag="HYI41_24660"
     CDS             complement(466..768)
                     /locus_tag="HYI41_RS24890"
                     /old_locus_tag="HYI41_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024139129.1"
                     /translation="MLKTVRSCLLSALLTHLFFGNIAVAKENLSDDKVKQAIIDESIAA
                     YPGTCACPFNSARNGSSCGRRSAWSKAGGYSPICYKKEVTKEMIKDWRASHSNNN"
                     /name="hypothetical protein"
     gene            complement(868..1296)
                     /locus_tag="HYI41_RS24895"
                     /old_locus_tag="HYI41_24665"
     CDS             complement(868..1296)
                     /locus_tag="HYI41_RS24895"
                     /old_locus_tag="HYI41_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001749513.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQHTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYMNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1377..1814)
                     /locus_tag="HYI41_RS24900"
                     /old_locus_tag="HYI41_24670"
     CDS             complement(1377..1814)
                     /locus_tag="HYI41_RS24900"
                     /old_locus_tag="HYI41_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001749514.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQYLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            2074..3237
                     /gene="cna"
                     /locus_tag="HYI41_RS24905"
                     /old_locus_tag="HYI41_24675"
     CDS             2074..3237
                     /gene="cna"
                     /locus_tag="HYI41_RS24905"
                     /old_locus_tag="HYI41_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="WP_040089861.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGSSAKAGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGEKYKNVA
                     KEVAGDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAQDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTVLGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            complement(3227..3769)
                     /gene="cni"
                     /locus_tag="HYI41_RS24910"
                     /old_locus_tag="HYI41_24680"
     CDS             complement(3227..3769)
                     /gene="cni"
                     /locus_tag="HYI41_RS24910"
                     /old_locus_tag="HYI41_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="WP_260479401.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSSNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSSLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYLWFFIGT
                     YKLFTREVYSPDTHVIE"
                     /name="cni"
     gene            3797..3934
                     /locus_tag="HYI41_RS24915"
                     /old_locus_tag="HYI41_24685"
     CDS             3797..3934
                     /locus_tag="HYI41_RS24915"
                     /old_locus_tag="HYI41_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_024139133.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     gene            complement(4123..4284)
                     /locus_tag="HYI41_RS25100"
     CDS             complement(4123..4284)
                     /locus_tag="HYI41_RS25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(4865..5089)
                     /locus_tag="HYI41_RS24920"
                     /old_locus_tag="HYI41_24690"
     CDS             complement(4865..5089)
                     /locus_tag="HYI41_RS24920"
                     /old_locus_tag="HYI41_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_089537792.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENLLTSPSGRVSRF"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5153..5475
                     /locus_tag="HYI41_RS24925"
                     /old_locus_tag="HYI41_24695"
                     /pseudo=""
     CDS             5153..5475
                     /locus_tag="HYI41_RS24925"
                     /old_locus_tag="HYI41_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5684..>6115
                     /locus_tag="HYI41_RS24930"
                     /pseudo=""
     CDS             5684..>6115
                     /locus_tag="HYI41_RS24930"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6153..6671
                     /locus_tag="HYI41_RS24935"
                     /old_locus_tag="HYI41_24705"
     CDS             6153..6671
                     /locus_tag="HYI41_RS24935"
                     /old_locus_tag="HYI41_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_157915313.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMILLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            6678..6911
                     /locus_tag="HYI41_RS24940"
                     /old_locus_tag="HYI41_24710"
     CDS             6678..6911
                     /locus_tag="HYI41_RS24940"
                     /old_locus_tag="HYI41_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4782..4869
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(4213..4758)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5466..7015
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttgtcataa acataaagct gccactaatg gcagctttat gagggtacga atacaggctt
       61 tattgaactt caatatcgat ctgatcaaca caagtctgtg cttggctgtg attttctgct
      121 gtgtaccact ggcctgattg taaggtgacc tcagttagac catctatagg aatgcagcgc
      181 cagttgaatg tggaaccagg tgtcacagta cctttactgc tttcaccgcc aaactgatag
      241 aaaagagcct gttttcgtcc tttttttgaa cctagcgcat gagggcacat ctcgcggtaa
      301 aaaccttggt aatatgctgt tacttgcaac ttttctttga ttgccttggc aattaaatca
      361 taattattag acacttagct tcaatctccc agagtatgat ttcctgttgg aaatatatgt
      421 atttatcaga aatgtaatgt aaacagaaag ttataaaaat agaagtcagt tgttatttga
      481 gtgagaagct cgccaatctt ttatcatttc ttttgttacc tcttttttgt agcagattgg
      541 cgaataacct cctgctttgc tccaggcgct gcgcctgccg cacgagctgc catttcttgc
      601 gctgttaaac ggacatgcac atgttccggg ataggctgct atcgactcgt caataatcgc
      661 ctgctttact ttgtcatctg atagattttc tttcgccaca gcgatattgc cgaaaaaaag
      721 atgtgtcagt aaagctgata acagacacga acgtactgtc ttaagcattt gaatagtctc
      781 cgaggaataa tcctaggaga attgcatttt tgtacaaaaa aacaacttaa atcgcttctt
      841 gttgttaagg gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac
      901 cgcagaaatc gtgtcagcca gcagcttcat tttttccggt gatggcatat cgctattcat
      961 gtattttctg cccgcaaagg tagttgtgct tctgagagac tgaagaaatt tttctgagtg
     1021 gaattctttt atgcgttgct gtgccgtttc tgctgatact ccggctgctt tgtctttctc
     1081 ccccatctcc ctgaactgct gaaagaacgg aatcatcatc cgtgtggtgt cggccttgtt
     1141 ggtgtagaag ttagggtcag aacggtcatc ggaagcgtac acataatgtc gggtatgttg
     1201 ttccacactg cctttcgggg tacaggccgt gagggctgca gccacagtta tcagggatat
     1261 aagatacaga tagcgtgtgc caccagaacg tttcatacgt gtctccttac cttaaagtat
     1321 ttattctcga tgagttatta tccgaagact atcttatcat cagcctctga ccgagttcag
     1381 tgaccgaaga gtctggacca gaatccccgt ttcttttcct gttccagtgc ctgcctgagc
     1441 tgggctatct cattctgtaa ctgttcccgt tctgcttcct ggtgcctgcg atccatatcc
     1501 tgtgcctgtt tatcctcaag catcagcgtc aggtattgtt tcagctcatt cagttcccgg
     1561 agaatgcgtt ctgtctgctg atcatgtgga ttttctgcct gtcccacagt gtgcctttct
     1621 tgtgtctcat tttgctttaa ttcgccgtat gcccggatca gttcactggt ttcaaactcc
     1681 cttcgtccgt cctggctggt acggtaactg acaagacctg agcgcatatc acggtaaaac
     1741 tgtgaacgcg atcttccagt cattttcagt gcgtcccgga gtgtgtgcca tgccataaag
     1801 ttacagtgtc ccataggtgt ctcatctcat ggtttcagta aaacataatg aggtctgaga
     1861 acggtaatgt ttgcgccggt ttttgtggta tccggcggga atagcgcgtg gtgtgagaga
     1921 ctgttttttt gatcgttttc acaaaaatgg aagtccacag tcttgacagg gaaaatgcag
     1981 cggcgtagct tttatactgt acataaaacc agtggttata tgtacagtat ttgtttttaa
     2041 tggtttgttt taaaagtcaa agaggggttt attatgggta gtaatggcgc agataacgca
     2101 cataacaatg cttttggtgg agggaaaaat ccgggcattg gtaataccag tggcgctgga
     2161 agtaatggta gcgcatcaag taaccgagga aattccaatg gatggtcatg gagtaataag
     2221 cctcataaaa atgatggctt ccacagtgat ggttcttacc atattacatt tcatggggac
     2281 aataattcaa agcctaaacc tggtgggaat agtggaaata gaggtaataa tggtgatgga
     2341 tcgagtgcta aggctggaga gataacaatt acacctgaca actcgaaacc tggtcgttat
     2401 atttcgtcaa atccagaata ttcattgtta gcaaaattaa ttgatgcgga atcaattaaa
     2461 ggtacagagg tatatacttt tcacaccaga aaaggtcagt atgtcaaggt tactgttcca
     2521 gatagtaata ttgataaaat gagagttgat tatgtgaact ggaagggacc gaaatataac
     2581 aataaacttg tgaagaggtt tgtgagccag tttttattat ttaggaagga agaaaaagaa
     2641 aaaaatgaaa aagaagcctt gctaaaggct agtgaacttg tgtctggtat gggggataaa
     2701 cttggcgagt atcttggaga aaaatataaa aatgtagcta aggaagttgc cggtgatatt
     2761 aaaaactttc atggccgtaa tattcgtagc tataatgaag caatggcttc actgaataaa
     2821 gtgttagcaa atccaaagat gaaagtaaac aaatctgata aggatgccat tgtgaatgcc
     2881 tggaaacagg ttaatgctca ggacatggct aataagattg gtaatcttgg caaggcattt
     2941 aaggttgctg atttagctat aaaggttgag aaaattaggg aaaaaagcat tgagggatac
     3001 aatactggca actggggacc tctcctgctg gaggttgaat catggatcat cgggggcgtt
     3061 gttgctggag ttgctattag tttgtttggg gctgtgttga gttttctccc aatctcagga
     3121 cttgctgtta ctgtgttggg ggtaatagga ataatgacga ttagttactt gtcatctttc
     3181 attgatgcaa atcgagtttc gaatataaat aacattatat ctagtgttat tcgatgacat
     3241 gagtgtcagg ggagtatacc tccctggtaa atagtttata tgttcctata aagaaccaaa
     3301 ggtataaata tgtgaatata tatatcccaa tgtatagtgt tatatagaag aaagacaaaa
     3361 acaggtcatt ttttgacatt aatacaaatg tttttgatga caaggttagt tctgtgtcag
     3421 ttagcaagaa acagtatatc atggatatat aaagaatagt gaaaggagtt aatatcttta
     3481 gcgcccgcag agatgatgac tttggtttta actctaattc tttgtgtgtg aatatgaata
     3541 agctgattgc agctaaaggg gctgtatgaa catagagatt gaatgcttcg cttaggggcg
     3601 ggttgtttga tgaatgaaat cctggtaggg ataggtatgc gatgattttt tcaaggagtg
     3661 gtattggtgc attattataa ctgaaaaata ttaatgcgaa tggggaaagt aagagcagaa
     3721 gactgaatga tatttttttt gatatcttat ttctgtcttt tatatccatg ttgctttctt
     3781 tggatggttg gaaattatgt gtgggaaaat tctattgatc ttgtttttta tcatgacact
     3841 tagtgcatgt caggtcaatc acatccgtga tgtgaaagga gggactgttg ctccatcatc
     3901 atcatccaga ctaacaggag ttgaaattaa gtaaaagatc aaaggatcct cttgagatcc
     3961 tttttttctg cgcgtaatct gctgcctgta aacgaaaaaa ccgcctgggg aggcggttta
     4021 gtcgaaggtt aagtcagttg gggactgctt aaccgaggta actggcttca gcagagcgca
     4081 gataccaaat actgtccttt cagtgtagcc gcagttgggc catcacttca agaactctgt
     4141 aagcatctgg ataaatctcg ctctgctaat ccggttacca gtggctgctg ccagtggcgt
     4201 taaggcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4261 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgggcc
     4321 gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4381 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4441 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4501 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcggcg cggccttttg
     4561 ttgccctttg cctttttact cacatgttct ttccggcctt atcccctgat tctgtggata
     4621 accgtatttc cgcctttgag tgagccggca ccgcttgccg cagccgaacg accgagcgca
     4681 gcgagtcagt gagcgaggaa gcggaagagc gtcggaagct gcattttctc cttacgcatc
     4741 tgtgcggcat ttcacacccg gcatggcgtg cttttcatac aatccgcact gatgccgcat
     4801 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctg cgccccgaca
     4861 cccgctaaaa cctgctgacg cgccctgacg ggcttgtcag caggttttca ccgtcatccc
     4921 cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga
     4981 tgtctgcctg ttcatccgcg tccagttcgt tgagcttttc cagcagcgtt aatgtctggc
     5041 ttctgataaa gcgcgccatg ttaagggctg ttttttcctg tttagtcact gatgcctccg
     5101 tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgcgaga ggatgctcac
     5161 gatacgggtt actgatgatg aacatgcccg gttacttgaa cgctgtgagg gtaaacaact
     5221 ggcggtatgg atgcggcggg tctgcctggg gggccggttg cccgttccgg gaggctgccg
     5281 acactggcac cgccgttact gcgtcagctg gccgccatcg gaaataacct gaatcagaca
     5341 gcccgtaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt ggtggccgca
     5401 ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga acagggggcg
     5461 cgggatgata gttaaatttc atgccagggg aaaaggtggt ggcagtggtc cggttgatta
     5521 cctgctgggg agggagcgta accgcgaagg cgcaacggtg cttcagggta atccggaaga
     5581 agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg gtgttctgtc
     5641 gttcgcggag aaggaactgc cgccgggagg acgtgaaaaa gtgatggcga gctttgagcg
     5701 tgtactgatg cccggtctcg aaaaaaatca gtacagcatc ctgtgggtgg agcaccagga
     5761 caagggacgg cttgagctga attttgtcat tccgaacatg gagctacaga ccggaaaacg
     5821 tctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc agacgctggt
     5881 gaatcaccat tacggactgc atgaccccaa cgccccggag aaccgccgga cgctgaccct
     5941 tcctgataac ctgccggaaa cgaaacaggc acttgctgag agcgttacac gcggtataga
     6001 cgcactttac catgccggag agataaaagg ccgtcaggat gtgattcagg cgctcacaga
     6061 ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag acccgaacgg
     6121 cgggaggaat atcaggctga aaggagcatt ttatgagcaa tcttttacag acgggcgcgg
     6181 agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg aacgacgagt
     6241 tcaggaagct cggcgagtct gtcagcgagg ctgtgacctc aaacgagacg aaaatcagag
     6301 acgctatagc cctgttcaca gcctcgacag agaaatcgct ggaaaaacac cgggaagggg
     6361 tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg aatacgggca
     6421 tgatattact ggggatagtc tttctcctgt ttaccgcgag tggcgggacg ctctggtatc
     6481 ttggcggacg gatacagacg aacctggaag aaatcaggaa gcaggaagag acattgcaga
     6541 aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga cgggaacagg aaattccttg
     6601 tccttccgta cgggaaatca gcggaggtga ttccctttca ggggaaagag tgggtacatc
     6661 tgaaggagtg acacagagtg acagagctgg aaatacattt gctgaacgcc ttagagcagc
     6721 tgcaacagga ctatatgcag cggctgagcg aatgggagag cgccttcgtg gaattgcaga
     6781 agatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc atgcagttga
     6841 gtcagcaggt gcagcacttg agcgagcaga cagaacgctt gagccagtta tacagcgaga
     6901 actggagata agagaggaac ggttgataca ggagcgcgaa catgtgttat ccctggaacg
     6961 ggagcgtcag ccggaaatac aggaacgcac gctggatggc ccttcgctgg gatggtgaaa
     7021 ccatgaaaaa tggcagcttc agtggattaa gtggggggaa tgtggcctgt accatctggt
     7081 tgcataggta ttcatacggt taaaatttat caggcgcgat cgcggcagtt tttcgggtgg
     7141 tttgttgcca tttttacctg tttgctgccg tgatcgcgct gaacgcgttt tagcggtgcg
     7201 tacaatcagg gctttatgtt aaatgctgat g
//
