LOCUS       NZ_CP060082             1240 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain G3/10 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP060082
VERSION     NZ_CP060082.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15693146
            Assembly: GCF_014295275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Whole genome sequencing of five probiotic Escherichia coli strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2020) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: UAMS_Ecol_g310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:39:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,044
            CDSs (total)                      :: 4,926
            Genes (coding)                    :: 4,666
            CDSs (with protein)               :: 4,666
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 80 of 260
            Pseudo Genes (incomplete)         :: 168 of 260
            Pseudo Genes (internal stop)      :: 50 of 260
            Pseudo Genes (multiple problems)  :: 37 of 260
            Pseudo Genes (short protein)      :: 3 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060082.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G3/10"
                     /isolation_source="feces from healthy individual"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany"
                     /collection_date="1954"
     gene            complement(272..781)
                     /locus_tag="H4P47_RS24855"
                     /old_locus_tag="H4P47_24855"
     CDS             complement(272..781)
                     /locus_tag="H4P47_RS24855"
                     /old_locus_tag="H4P47_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634347.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023206179.1"
                     /translation="MIVASEHQAKLRQKEIQEIDKKASKEHEEAKKNSNFTQTYPKGWQ
                     RIRELVRDKQGAAAIGLYSFFAEHIDPTCGAVVCDQQFLANQMGVSTRTIRRWLDYLEE
                     KGALVRIPVAGKVCAYALDPHEVWKGYNTSKDYSAFVTKTLVNKDGEIKRRIMAMFSGE
                     ESGQEQ"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 atcgttatga gactcccaat taagccgatg attgtgatga ttgttgacgt gatcatgatg
       61 acctccttgt cggtaaagtt tctgtattac attcctggct aacttctttt agtcccaaag
      121 gaatgccgca cattacaaaa gtgatgtgct gttttattta tttatgggat ctactgctta
      181 aagggcggct tattatgggg atggctcgct gtggcgaacc atcccccttt ctgagggtgg
      241 gggtcgttcc gagccgtcaa gagtcgcttc gctactgctc ttgaccgctc tcctctccgg
      301 aaaacatagc cattatccgg cgttttattt ctccatcctt gttcactaat gtcttggtaa
      361 caaaggctga gtagtctttt gatgtgttat atcccttcca gacttcgtga ggatctaaag
      421 cgtaagcaca aacctttcca gcaactggta ttcttaccaa agcacctttc tcctccaaat
      481 aatctaacca acgacgaatt gttcgagtag aaacacccat ctgattagct aaaaactgct
      541 gatcgcagac cactgcaccg catgtaggat ctatgtgttc agcgaaaaat gagtaaagac
      601 ctatagcggc agcaccctgt ttgtccctca caagctctct aatgcgttgc cagccctttg
      661 ggtatgtctg agtgaagtta ctgttcttct ttgcctcctc gtgctcttta gatgccttct
      721 tgtcgatttc ctgtatttct ttctgcctta gctttgcttg gtgttctgaa gctacgatca
      781 tctttatccc tcggtgtctt tttgatagga catttttgtc ccatcatagg acttgtggtg
      841 tcctatgtcc agccacatgc ggacaccagc tgtgtcctca cgtgcggaca ccagctgtgt
      901 cctatcagaa aaaaccaaaa ctataaaaat caataactta ttatttatct ctattctctt
      961 ctatgaaaac caacagacaa caccaaagtt caaggtagct ccctccctcc ggtcgggcat
     1021 tttgagggtc agggttcgcc ataggcgacc gcgttagcgg cttgaccttg acgatcaaaa
     1081 tgcccgttta accttgtatc tggtgatggc tcgcgtagtg agccatcacc atgattgacg
     1141 gttttcaggg gtttcggggc gaagccctga accagtcact ccgctagcgg agtgtatact
     1201 ggctaattag tgcttatcct tcgggtatat ctctcggtaa
//
