LOCUS       NZ_CP060081             1304 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain G3/10 plasmid pSYM4, complete sequence.
ACCESSION   NZ_CP060081
VERSION     NZ_CP060081.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15693146
            Assembly: GCF_014295275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Whole genome sequencing of five probiotic Escherichia coli strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2020) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: UAMS_Ecol_g310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:39:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,044
            CDSs (total)                      :: 4,926
            Genes (coding)                    :: 4,666
            CDSs (with protein)               :: 4,666
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 80 of 260
            Pseudo Genes (incomplete)         :: 168 of 260
            Pseudo Genes (internal stop)      :: 50 of 260
            Pseudo Genes (multiple problems)  :: 37 of 260
            Pseudo Genes (short protein)      :: 3 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060081.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G3/10"
                     /isolation_source="feces from healthy individual"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM4"
                     /geo_loc_name="Germany"
                     /collection_date="1954"
     gene            1..531
                     /locus_tag="H4P47_RS24850"
                     /old_locus_tag="H4P47_24850"
     CDS             1..531
                     /locus_tag="H4P47_RS24850"
                     /old_locus_tag="H4P47_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_137445334.1"
                     /translation="MISVLDKRSQRRMREIDLERAKEAQERREDQKNSKFTQVSPKGWE
                     RIRELSKDKQGVAAIALYSFLAEHIDPTCGAVVAEQQFLADKLNVSRSTIKRWLNYLET
                     KNAVVRIPVAGNVCAYALDPHEVWKGYNSSKGYAAFVSKTLVNVDGDVQRRIVAMFKGQ
                     EGDAKDQCDLPLE"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 atgatttcgg tgttagacaa acgatctcaa agacggatga gagagatcga tttagagcgc
       61 gctaaagagg cccaggaacg gcgtgaagac caaaagaaca gtaagttcac ccaagtatcc
      121 ccaaagggct gggaacgcat tagagagctt tctaaggaca aacagggtgt ggctgcaatc
      181 gcgctgtact cgtttttagc tgagcacatc gatccaacgt gtggggctgt cgttgctgaa
      241 caacagtttt tggctgacaa actgaacgtt agcaggagca cgatcaagcg ttggttaaac
      301 taccttgaga ccaaaaatgc ggtagtaaga atcccagttg ccggcaacgt ctgcgcctat
      361 gcgctagacc cccatgaggt ttggaagggt tacaactcca gcaaaggcta tgcagcattc
      421 gtaagcaaaa cgttggttaa cgttgacggt gatgtacagc gccgaatcgt tgccatgttc
      481 aaagggcagg aaggcgacgc taaagaccaa tgcgacctgc ctttagagtg aaagccggtc
      541 aagggcgcgt agcggcgtag cgcacccttg acgggctgta acgaccccca cacttggcaa
      601 aggtggttgt gatcacgcag tgagcgcaac cacctgttaa gccgcccttg acgttgacct
      661 tccgggtaaa aaacactgcg atgcaggata gggcaaacgc cgcaaaatga cgtcatccga
      721 catcattccg ccgcgttcgc ccagtgacct acagtcggct ctggggtgtc gaggctgtgc
      781 cctgaccagg cgttttggag tatcgccacc gtggtggcgg tgctacaaaa catatcctgt
      841 cccttccttg gcatctctga agtttagcca gtatacactc cgctagcgct acgtgactgg
      901 ttcagggctg cgccccgaaa cccgcaaagg tcaaaaccaa aagcgtctga caggtggttg
      961 cgctcactgc gtgatcacaa ccacctcctg gcagcatgct gggcaatttt gagcgtcaag
     1021 gtcgggccgc ttcgcggtcg ctaaagcgaa ccttgacccc caaaaatgcc cgccgaaagg
     1081 cgggaggtaa ctttagccct gggtgttgaa tttaggtttt taaagattag ataagcggaa
     1141 aactataagt atctgattta tatacatttc aataaatgag ctagaccaca catgcgtgag
     1201 ctagctctag accatgcatg cgtgagctag cttgagctaa gatagtttac ttttgagcta
     1261 aggtagctta tttttgagct accaatggct catgaggaat agcg
//
