LOCUS       NZ_CP060068            12706 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain G5 plasmid pSYM9, complete sequence.
ACCESSION   NZ_CP060068
VERSION     NZ_CP060068.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15693148
            Assembly: GCF_014295295.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Whole genome sequencing of five probiotic Escherichia coli strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2020) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: UAMS_Ecol_g5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 696x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:46:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,964
            CDSs (total)                      :: 4,845
            Genes (coding)                    :: 4,610
            CDSs (with protein)               :: 4,610
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 88 of 235
            Pseudo Genes (incomplete)         :: 147 of 235
            Pseudo Genes (internal stop)      :: 43 of 235
            Pseudo Genes (multiple problems)  :: 42 of 235
            Pseudo Genes (short protein)      :: 4 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060068.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G5"
                     /isolation_source="feces from healthy individual"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM9"
                     /geo_loc_name="Germany"
                     /collection_date="1954"
     gene            complement(743..1210)
                     /locus_tag="H4P49_RS24370"
                     /old_locus_tag="H4P49_24370"
     CDS             complement(743..1210)
                     /locus_tag="H4P49_RS24370"
                     /old_locus_tag="H4P49_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064186840.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            1428..1682
                     /locus_tag="H4P49_RS24375"
                     /old_locus_tag="H4P49_24375"
     CDS             1428..1682
                     /locus_tag="H4P49_RS24375"
                     /old_locus_tag="H4P49_24375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_187002624.1"
                     /translation="MLLTPVRLSLWSCFPCVKADDADGAVSPCLVAEGSLVLASAVGGA
                     MTRLAKLADLAVFDAQARVAGRGGLLYALHVIKPLTGGW"
                     /name="hypothetical protein"
     gene            complement(1763..2350)
                     /locus_tag="H4P49_RS24380"
                     /old_locus_tag="H4P49_24380"
     CDS             complement(1763..2350)
                     /locus_tag="H4P49_RS24380"
                     /old_locus_tag="H4P49_24380"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_064186842.1"
                     /translation="MNKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVDLS
                     SAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            2610..3269
                     /locus_tag="H4P49_RS24385"
                     /old_locus_tag="H4P49_24385"
     CDS             2610..3269
                     /locus_tag="H4P49_RS24385"
                     /old_locus_tag="H4P49_24385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117150913.1"
                     /translation="MTEIKTECPDAAWLRTTLAEMASDHFPVYDLPSLRVDPNSSTQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            3452..3841
                     /locus_tag="H4P49_RS24390"
                     /old_locus_tag="H4P49_24390"
     CDS             3452..3841
                     /locus_tag="H4P49_RS24390"
                     /old_locus_tag="H4P49_24390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_187002625.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVHAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            3834..4196
                     /locus_tag="H4P49_RS24395"
                     /old_locus_tag="H4P49_24395"
     CDS             3834..4196
                     /locus_tag="H4P49_RS24395"
                     /old_locus_tag="H4P49_24395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117150911.1"
                     /translation="MRELPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
     gene            4189..4374
                     /locus_tag="H4P49_RS24400"
                     /old_locus_tag="H4P49_24400"
     CDS             4189..4374
                     /locus_tag="H4P49_RS24400"
                     /old_locus_tag="H4P49_24400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162780107.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRREQPRSTGR"
                     /name="hypothetical protein"
     gene            complement(4394..5035)
                     /locus_tag="H4P49_RS24405"
                     /old_locus_tag="H4P49_24405"
     CDS             complement(4394..5035)
                     /locus_tag="H4P49_RS24405"
                     /old_locus_tag="H4P49_24405"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_117150910.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKATKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIREFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(5032..6321)
                     /locus_tag="H4P49_RS24410"
                     /old_locus_tag="H4P49_24410"
     CDS             complement(5032..6321)
                     /locus_tag="H4P49_RS24410"
                     /old_locus_tag="H4P49_24410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142910546.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRAPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            6677..6886
                     /locus_tag="H4P49_RS24415"
                     /old_locus_tag="H4P49_24415"
     CDS             6677..6886
                     /locus_tag="H4P49_RS24415"
                     /old_locus_tag="H4P49_24415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142910545.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRASDGVR"
                     /name="hypothetical protein"
     gene            6873..8054
                     /locus_tag="H4P49_RS24420"
                     /old_locus_tag="H4P49_24420"
     CDS             6873..8054
                     /locus_tag="H4P49_RS24420"
                     /old_locus_tag="H4P49_24420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117150907.1"
                     /translation="MAFDNSHLADPGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSARHNRGSAHDRSQYRHFLKLKGVTNPEAMAQVFLQFSGEVYWRLDSKR
                     TPPTPEQVAALADRKRRSIEAVARREDADRRKAEKAAADAVRMLAKGDGNPAYTPYWRN
                     KTTIARRGGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAI
                     CGVCSAGGKTGDKFSPAGTQKAGRFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALH
                     HLSRGKVAVCAAFSIAGFGSCVASLRRFWPDVEVMLVPDAGEAYRLAHKAAYGDDREPV
                     PTIPGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            8280..8735
                     /locus_tag="H4P49_RS24425"
                     /old_locus_tag="H4P49_24425"
     CDS             8280..8735
                     /locus_tag="H4P49_RS24425"
                     /old_locus_tag="H4P49_24425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101998989.1"
                     /translation="MKSKAELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNALATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDEAA"
                     /name="hypothetical protein"
     gene            complement(9117..9608)
                     /locus_tag="H4P49_RS24430"
                     /old_locus_tag="H4P49_24430"
     CDS             complement(9117..9608)
                     /locus_tag="H4P49_RS24430"
                     /old_locus_tag="H4P49_24430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(9673..10164)
                     /locus_tag="H4P49_RS24435"
                     /old_locus_tag="H4P49_24435"
     CDS             complement(9673..10164)
                     /locus_tag="H4P49_RS24435"
                     /old_locus_tag="H4P49_24435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117150916.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGK
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQVAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            10675..12462
                     /locus_tag="H4P49_RS24440"
                     /old_locus_tag="H4P49_24440"
     CDS             10675..12462
                     /locus_tag="H4P49_RS24440"
                     /old_locus_tag="H4P49_24440"
                     /inference="COORDINATES: protein motif:HMM:NF017826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="WP_181959639.1"
                     /translation="MSNHEENSGAVADNVTPIDAAKGKPAADGSDLPFIRFSGGWVYLK
                     TCKTDKKGETYYSLQAVCKSHLRVIGTGWVDGEAFRVLELGKDKRRVMMPTRLIGSRDG
                     WAFLQGHNVQVTPVPELRRWLHEYLMSETAYDLHGSPCGVLPEWDVADKPGWRGGAYLL
                     PDGDMIGDVGNVIADFKLRSNHADVGVCGTVDSWVENVGRLLYGNDSPTLALGVAMASP
                     MLGVLGVSGFGVHLYGQSSGGKTSSCYPAMSIFGSPSNRQASWNSTPLALTLLARCCND
                     ALMMLDEMKLLRAMVMEEAIYGTFSQRSRMQGNKDGTLRPEYSWRCCVLSTGELAADVF
                     IRMMTGRDADAGALVRLVQLPYRPLTNLHGFPDGSAFADHLRAASDEHYGAVGRAWVDW
                     LSSNQTLAREKFAEVKARWNGRGTHGQHQRVADYFAVIETALTLSSKTLKLDVKECQGV
                     LDRFYADWLVDFSKGGNGSHEEALLVERLEAVLSQHQRFLSKTANDQDYKQPGELWGYF
                     HDRGDAREYLLFPAVFKERISGTIDAKVAGRLLHTRGLLRRHGEHLTVSERAGGYPNKR
                     RFYALSLPVVDDLDAEEVL"
                     /name="DUF927 domain-containing protein"
     gene            12462..12647
                     /locus_tag="H4P49_RS24445"
                     /old_locus_tag="H4P49_24445"
     CDS             12462..12647
                     /locus_tag="H4P49_RS24445"
                     /old_locus_tag="H4P49_24445"
                     /inference="COORDINATES: protein motif:HMM:NF013937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_117150914.1"
                     /translation="MAKPTPAESAMNVTRFIRAQTLVMLEKVEALDFDDLTALAEKLHD
                     DAEKLERLVTDRVKNG"
                     /name="Rop family plasmid primer RNA-binding protein"
ORIGIN
        1 tgcgggcttt ttcatgcaat tcacccgtaa aatcgaaaaa ctactggttc cgcactggtt
       61 ccagctacag gccgcgccgt tgctgggctc cagcgatttg gaaccagtgg aaccagtgga
      121 accggtgggt tgtttgtctc tatggggatc tcccccaagt tgcggcaatc aggaaagtct
      181 atgcgcacat acgtgtatta ttttttactg gttccactgg ttccactggt tccagtgtcc
      241 tctaaggcgc gtggttgctg ggtttgtttt ggaaccagtg aaaaataatg actggttcca
      301 gactggttcc actggttcca ctcggtgggt gtaatgggtg taatggttaa aacctgcgca
      361 gtcgccgtca cgcagctgca ccccgttggg ctgcactttt gacgctatgg gggatctgtc
      421 gatgggttcg tcgtgccggg gtcgtgggtg gtggtggttc ggagggggat cggtggtttg
      481 ggtggcgctg agggcacgag ctgtcgctcg cgctgggtgg tcgtctgttg gttctgcgtg
      541 agggcttgcc ctcgcgccct ctgcgttatg ggtggaatgc gttgctgcag ctgcgcgttt
      601 ggttcgtccg ggcatcaaaa cggggcttct cgcgcacgcg agcggtaaca attgcgtaac
      661 gtgcggatcc gcgtattccg tgggctcccg ggttttggcg gtcacaaaaa cggtcacatg
      721 gcggtcattt cggtcatggc ggtcattgtt gtttgcggtt catgccctcg cgcatgatgt
      781 cggcctcggt gcccggcttg atctcgtaga tgaggcgttc gcgttcctgc gctttgagca
      841 gggcggtcat gaggccgctg atcgctttga ccatggtggc gtccttctcc agcatggcct
      901 gtaacgatgc gctgcggagg gcttgcaagt cctttgcgtg gcgctgtagc aggtcaaatt
      961 gctgctgtac aaagtggttt ttaaggcgtc ggatcgcggc ctgtggtgtg gcctcgacgg
     1021 cggcggcggc gcgtttccgc atctcctcga tgctgaggac gttgctgggt gctgcggcct
     1081 tctcgctcgc ccatcggcga acggtgcggg gtgggatccc catggtgcgt gcgacggtgg
     1141 cactggttgc gccgtcctgc tccagtagcc tgagcgcctg ggctttctgt tcggtggtgt
     1201 atttcttcat ggttcctgct ccggttggtt tctctcccgt attgaaacgt cacgcgctga
     1261 aaatggcggg ggtttttggg gggtacacgg cgtttgtgcg tgcgtggtcg tgcggttggg
     1321 tgtttttgtg gctgaggcgc ttagaatggc tctggtgcgt tctggtgaat ggggcgcttc
     1381 cgcgcccctg taattggttc gtaatggcgt aaggcttttg ttattgcttg ctgcttacac
     1441 ctgtaaggct ttcgttatgg tcatgcttcc catgcgtaaa ggctgatgat gctgacggtg
     1501 cggtatcgcc ctgcctggtc gcggagggta gtctggtact ggcctcggcg gttggcggcg
     1561 caatgacgcg gcttgccaaa ctggcggatc tcgccgtgtt cgatgctcag gcccgggtag
     1621 cgggcagggg tggtctgttg tatgctctgc atgtcattaa acctctaacg ggtggttggt
     1681 gatgcccggg ttggctctca cacttccccg ggcctgggtt gcggcggtga ctttagcgag
     1741 taccaccgcc gcgatctctc tcttactccg gcgcgaccgc gtcgaccttc tcaagatggc
     1801 ggtaaatcgc gctccggctt aggtttagct gtttggctat ggtggcgatc ggcactccca
     1861 tggcgcggcg catgacaatc tccccggctt ccttcgctcc gatcttggtc gggcgtccga
     1921 acttctggcc tcggctcttt gctgcctcga tacctgctgc ctggcgttcg aggatgagct
     1981 tgcgttccat ctgggcgaag ctggccatta cgccgagtac cgcctgtccg acggcgctgc
     2041 tcaggtcgac gccctcgcgg atgctgacca gggtgatctt ccgcttctcc agctcctcga
     2101 ccagctcgag ggtgtccctg gcgttcctgc cgatgcgatc gatcgcgtaa atgaccaggg
     2161 tgtcgccctc ctgcagcaac tggttcaaga ggatgccgag ggcagggcgc tcgcgtggtg
     2221 gtaccgtccc gctgatcccg tcgtcggcaa ggatggaggc ggttgggaac cgggcgatga
     2281 tggcgtctcg ctggctgttg ctggtttgtt cgtcggttga tacgcgaacg taaccgtatg
     2341 ttttgttcac ttttttctct cctctgaccg atacgtctct ttcgacacaa catgccacaa
     2401 ctgtatcgaa agtctatcgc taaagtgtct ttttcgtcgt tctgttgggt gtcctgaaat
     2461 gtaggggatt agcgacggcc tccaggcggc tccccgcgct gggcgagagc cctgcgccct
     2521 ctgcgttgat gtctgcgctg cgtcgtgacg gcatgctgtc tgagcggcgt ttcttgccgc
     2581 tttgctgata acttgctgag aaccaaacca tgaccgaaat caaaaccgaa tgcccggacg
     2641 cggcctggct gcgtaccacg cttgctgaaa tggcgagtga ccattttccc gtttatgacc
     2701 tcccgtccct gcgggtagac ccgaactcct ccactcagct ttcggcgctc gctgatcgtc
     2761 aggctgcgcg tgaaatgcgt caggccgctt ccgacgtaga ggcgcgacgg ctggacgccg
     2821 ctcgagtggt ggaagggctc aagaccgagg ccgaacggct gcggggcttg atcgccgacg
     2881 gcaaggctgc gctgcgtgct ggcgagccgg tgagccccga cgcgggtgtt gcgtcgttcc
     2941 tgctgccgga catcgaggcc gagctggttg ttgctgaggc tgctgaggcc gacgtggccc
     3001 gggagcgtga caccctgctg caggatgccg atcgccgtga cgcggcggct gcgctggcgc
     3061 tgttcaactg ggcgcactcc gttcgcgtgc agcgtatcga gctgcttctc aagctggcga
     3121 tggacgaggc gacgacgctc gctgaaaccg acggcgggcg cggcctgtac cgtaccgtga
     3181 tcgcgcctga ccgccgtctt aaccaaattt tcgccacgca gggcgctatt gaaatcctgc
     3241 gccgtaaccg tggcatggag ggtgtttaat gtctgctttc aaactcctgt gtgaccggct
     3301 tcgcaatgag gcgacgctgg caaaatctga cggggccggc gctttcaaaa agcaatctct
     3361 ggatcccgac ctgaagggcg gcaagaaggg caaaaagccc gccggggagc tggaggagga
     3421 cgaggcgatc gcgtctctcc accgtaagcg catgaacgac gttaaaggtt gcaagacgct
     3481 gaccaaatcc accggcgctg atgactttgg cctccctccg ctgtcgtcgg tggtcgagac
     3541 agtggtggag acaggcggcg cagcgcagac gttcgcaccg atcggggctc cggtggcgtc
     3601 tccgaccgaa gcggttcacc gtgcgcgtgc gcagtccatc caggacgcgg cgttcctgaa
     3661 cgagatgaaa gcggcgggtg tggcaccggc tgacctggtg ttacttgacg gcgtgttgac
     3721 gtcctctcgt gggatgccag acgcggcgcg tgctccggcg tttgccgagg tgcgccgtca
     3781 ggttcatgcg ggcggcatga cgctgtcacc ggctgcgcgt ggtattgact ggaatgcgtg
     3841 agctgccgcg tcacctgcag ggcgaggtgt tgatgccgct gtccagcagg atcacgcgct
     3901 cgcaacggct ggagctgatc tggctgttgc ccgttctgcg gctccacccg ggcgatcgat
     3961 cggtcactga gcggcgggcg cttggcaggt tccggggatc gctcgctgcg ggtggccaga
     4021 cggatgcttt cgtccgttcg gatgcgtctc tgtacgcgct gggactgcgg ttccagcgca
     4081 agcgcacccg ggagctggtt gcttcggatc ccgatgttgc tgcggctggc gaggcgctgg
     4141 agcagcgtgc aaacggcgtg aacctgtccg agacgttcgg gggtggcgat gagtgaattt
     4201 gctgtctcgc gcctggtcat tcctgaggtg gtgatccccg acggggtcat tgctgcccga
     4261 cgggcaaggg gacaggctca acgtgtcgca gctgctcgcc gtgctgcaga gcgtcgtgct
     4321 gcgcctacgt ggcgctgggt gagacgggaa cagccccggt cgacagggcg gtgaaacggt
     4381 gagacggggc gcgttatgcg cccttttttt cgcccttcgc ctggcgtgcc ttgcgccgga
     4441 attcccggat ctctgtcagg cgggctttgg cgttttcggc ctcctccggg gttgctggtt
     4501 cggtggggtt gccgtccagg tcgtagcgca tgcactggtc gatgaggctc tgctggtacg
     4561 agctggtacc cgtgacaatg cccaggatgc gacggacggc tttgcggtct gcctctggcg
     4621 gcatgtccag ctctgcgcct cgggtcagga tctgctcgat gatgcccttt gccatggggc
     4681 gacggtcaac tccccgggtc agtaagtcag gccagtgctc tttcaggaat gctttggtca
     4741 cccggttggc cagcgccttt ttggacttgt tggcgatcgc gatgtcctgc tggcggcggt
     4801 cgttctcctc ctgtctgcgg atcccctcgg cgacctgctc gggcgtcttt ttcggctttg
     4861 ccgctgtggg cttctgtgcg ggcttctgcg gggctttcgc ttttggctgt accttcgcct
     4921 tgctggtagt ccgttcggct ggctgtggct cagatttcgt ggccttttcc ggcgggacgt
     4981 agaccgtgcg gttgcgtcgc ttgatggtca ggatcgggcg ggtgggttcg gtcataaatc
     5041 tctctcaggt ctgttggtct ggatgtggtc ggcaagggcg atcagggtgg cggcgtcgcc
     5101 gcggtcccga tcgatgcgcc ggatgatgtc gttgatgtag tcccagcgcc cgacggcggc
     5161 aaggacggaa agggtgaggc ggttgaaccg actctctggt acccggtcga gggtcagggg
     5221 gatgcgcttg cgggggcgtc ctggaccgcg ccctgtccct ggtcttttga gcagctgcgg
     5281 ctgctccctc tgcggcaaaa taacggggat cttcgccagg ctgcgcgtct cgttctgcgt
     5341 gaactcgggc accgcgatgt gctcgtctgt ccatgtcctg cctccgggcc tggctgatgc
     5401 gctggagtcg caggacgggc aggagacgac gatctcccgt cgcggcttgc ccttgaccgg
     5461 tgccttgctg atcaccaggg gcgcggcttt gcaatcggag cagaccgacg ctgtccagac
     5521 cgcgttgtct ggcagggtgg cgttccggta ctggtgcgcg gcgtcatgcg gtgtctggaa
     5581 gctgaccgtc tgaaacgtgg gggcgacgct gaggggtgcc gcccatggct ggcacctggg
     5641 acaagcaagc tccacctctc cggcgtgccc gtcgccaccg ttccagcgca ggggctcgcg
     5701 gtgttcgtgg cagtagtaca cgaaaccgtc gcggcgtgtt tgcccgacag ggcctggtcg
     5761 gcgatcgtgg aaccgtgcgc ccgggttggc tcgcatggcg cggacgagcg cctcgccctg
     5821 gtggatgccg taccagacct gccagccatc gccgatgccg atcaggaacg gcgagacgaa
     5881 cggtggggcg cggttgactc tcccgcgtgc gttggctccg atggtggctt cgcgttcgcg
     5941 gttccagcag gagacgcaca ggctcgaacc gggcttattg ggggcgatca ggcggttgtc
     6001 tttccggctg caacgggggc agagcttacg ggtgtctgcc ggggtggtca gtggtgcgtc
     6061 agggggctgt tcggtaccga cccaaagcgc gaggccagtg gggcaccggt ggcactcgag
     6121 gtgggggttt tcaatcgtgc gatcgaggtg gctctgctca taaatctgtc ggcatgcctc
     6181 gacccggagg gagcacccgc cggggcaggt gacgaacaaa acgccgggga gctgctcggg
     6241 gaatttttca gtcgcgctta cgcgttcaag tgctgccggg ttcggctgcg tcgattcgtt
     6301 cgtgtcgtta tcgtcgttca ctgctccccc tgcgtcgaaa tggtcgccag acagcgccag
     6361 cactggctgt gcgtcttttt tagtatctca caaaaaacgc cactttggcg ttaattgtgt
     6421 tacgcttgtc ttactgattc ctgacgagca gttgggaaac atgaaaccgg atcgcccggt
     6481 gccgctttcc ctcctcccca acgaggcggg gttgtaaccg aggatgcgcg cttcgtcctt
     6541 ggcaggtgcg agtaagcgag aagggctgct gtcctgccgc tatgcggtaa gtcctcaagg
     6601 gcggctttat cgataatcgt cactcccgcc ccccgatgcc cgttaagctg aggggggcac
     6661 ctatctggta ctcccaatga aaaaacgaat ctataccgct gacctcgctg cgctgctgaa
     6721 cgtgtcgctg cgcacgctct cgcggctaag ttcgcggggg atcgtgccga ctccggcagg
     6781 gcgctccctc cgtggcgggc attactggct ggctggcaat cccgagatca aacgcttcgt
     6841 tgccgaccag cgtcgtcccg ggagggcttc tgatggcgtt cgataactct cacctggctg
     6901 acccgggcct gttctggcgt gctgtgtctg ctgcgggggc tccggttgac cgtgaggact
     6961 ggctgcgcct ctgctccggc atcgcgtgct gggcgtctct ctgcggcgag gatgggctgg
     7021 cggctatcga ggcctggtct gcccgccata atcgcggctc tgcgcatgac cggtcgcagt
     7081 accggcactt cctcaagctg aaaggcgtaa ccaatcctga ggcaatggct caggtgttcc
     7141 tgcagttctc cggcgaggtg tactggcggc tcgactcgaa gcgcacgccg ccgacgcctg
     7201 aacaggttgc tgctctcgct gaccggaaac ggcgatcgat cgaggcggtg gccagacgcg
     7261 aagatgccga ccggcgcaag gccgagaagg ctgcggctga tgcggtcagg atgctggcga
     7321 aaggcgacgg taatccggcc tataccccgt actggcgcaa taagaccaca atcgccaggc
     7381 gtggtggcgt cagcctcccg cccctccccg ctgaccttcg tgtgggtggc ttgttccgtt
     7441 cgttcgaacc gagggcgtac tggtcgcgct gtctgcttgt tcctgcctat cgcctgaccg
     7501 acgatcgcca gctggttggc gacgcgctgg aggccatctg cggcgtctgc tctgctggcg
     7561 gcaaaaccgg cgacaagttc tcccctgcgg gtacccaaaa ggcgggacgg ttctatccga
     7621 tgcccggctt tatggacgtg ctcgacggct ccccgcacgc gccctactcg cctctgttcg
     7681 tctgtgaggg gttcgctacg gcgttcgccc tgcaccacct gagccgtggc aaggtggcgg
     7741 tctgcgctgc gttctcgatc gccgggttcg gcagctgcgt ggcgtcgctg cgtcggttct
     7801 ggcctgatgt tgaggtgatg ctggtaccgg acgcaggcga ggcgtaccgg ctggcgcata
     7861 aagcggccta tggcgacgat cgcgagccgg tcccgacaat tccaggggcg tcggtggtgt
     7921 cgttcgacgg cctgaaccgc ttcgagcccc gcgataacct cgactggttc gatgtgctgg
     7981 ttgaggaggg cgaggatcgg gcgcgtgagc tgctccggta tgcggtgcgt catgcccggc
     8041 ttgagcgggg ctgattggcg tgtccgaatg gtgcgccttt cgggacatct tttttgccgt
     8101 cgtggcgtct ggtttagcga acgactttcg agacactacc gatgcttttt tgatgagacg
     8161 gactaaagcg acgcaccggc gctgccgtcg tgacgctatc ctgcgatcgg tttctcacag
     8221 cagcataaag cccggcctcg tcttgatgtc gggctttttt ttagcccaaa ggagggcaca
     8281 tgaaatcaaa ggctgaacta cgcgaggccg cgacggctcg ttcgctggcg gtgatcgcca
     8341 ctgaaatgtc ggagtggtcg ctcgacgggt tctcccacct caagctgccg aacttctccg
     8401 ctggggaacg ccagcagacg ctgtccgggt cggtggtggt tgatcgcccg ccgttcgact
     8461 acgagtgggc tggtaccgag aaatttaacg cgctggccac ccgtgcgctg caggtgaaac
     8521 ttccggcatc gcgtgagcgt aactacgcct ggttgtgcgg cgttgaacgg gagacgctgg
     8581 cgacggcgct cctcgtggag ctgttctccg tcaccggctg cgtggcgttc gccgggctgg
     8641 gtaaggttgc cgatctggcg ttcctgacgc tggacgagag cgaggcgggg cagatcaggg
     8701 cggcgatgct gcagtggctc gatgaggcgg cgtaagccgt ctctcctctg cgtcaaatca
     8761 gaccgcgcta gtagcagcaa aaaagagaag aaatccccgc gagaccgctc ctcgtgccgt
     8821 ctggtcataa acaaacctgt tgaatctacg tcaacaatag cctactatta ttgtactgca
     8881 agagcattcg cagggggtat taaccagtaa ttaacccttt gcgctgggca gtcaggaggc
     8941 tggtctcctg ttgggaagtt atgttgcggc ttcctagtaa taacagtgaa tttgatagac
     9001 agcagaaaac cgccgatgct gctagatccg aaaggaacca atgaagggaa atatttaggc
     9061 gttatggttc attctcatta aattgaggag aattgcttaa atgggcgtcc ttcctatcac
     9121 cctcgctgta tagctttaca tttggcctcc gttgcatggt atgcagtcac gggggccaaa
     9181 ttattttcag agacatcaac tatcgccatt gcgaaaaagc cagccccagc aacgttaatg
     9241 cgacgaacag cattgagcac aatagtctgc tccgagtaat ctctgttaac gtacacattg
     9301 ctctgcaccg agaatgcagt tgccaaaatc tccgcacact cttgccaact tagcttatcc
     9361 ttcgagttac gagcagatag cacatgccta aacattgccc aagtgatcgg tattgctggt
     9421 gatgcaggta tgtcccagcc gtttctctga caaacaactt tcatttgatt gaactgcttc
     9481 ttagtcatca gataggtaaa acaaggcgtc tcccaaactt cctgagggag acctttatca
     9541 tccatttctt cagcttgctg tgtaaaagca tagaaagcgt cacggatagc atcatcaccg
     9601 atagccactt cttttccttg taaaattttt atgttcgaat tttatcatat ttagctatgt
     9661 cacttaggac tgctaattca gtagcccact ttcccgcaac ttatggcgat agtcccggac
     9721 acggctggtg ttccactcct gtcccgtcgc tgtccggtgc cctgctgcgt tcagcgccga
     9781 cgcgatggcg ggcagagtcg cgttctctgc agtcatgctg acaatgagct ggtcggcctc
     9841 atcactgacg gcctggaagc ggggatcggc tccccggggt actatctgtc ttgcaggttg
     9901 ttgtgcgctt tgtggtgctg tttgttgtgc gacttgtcgt gcaggtttac cgccgacgga
     9961 cagcgccctg acttcagccc tcagcgcttc gatggcagct aaaagtttgg tgtccatccc
    10021 ttccggcttc ttgccaagtc cggcgttcag cgcctctatg acagcatccg tgcgggttgc
    10081 tccggttcgt gctgcgttct gctcgatacg ttcgatcagg ctaacgggta ggcgaacggt
    10141 cagggttttg gttttctctc tcatggctgc tgctctccgt gtcgtgcgtt ttgtcgtgct
    10201 gtttgtcttg cggagtgtaa tgcgttttgt cgtgcgcgtc gaatttgacg tagtgcaatt
    10261 gtcgtgcagg gaggggtagc ggctcggccc gtggataact tctgtggata acaaaatttc
    10321 gaacgttccg gcccgtggat aaatcgcgga ggctgcgcgg ctctaagatc ggtgcgggat
    10381 cgcgtttcgc gtggttggat cgttgtgtac tggtgcaggg gggcatgtaa cggtatgatg
    10441 gcgcggctga aaaagaaaac ccgtccgggc agacgggtcg ttttcttaaa gctgttttga
    10501 gtttggagct ttcgcggcgg tcaccgtgga agacgccata ataactcatg ttgggtgtga
    10561 ctccagcatg aattatacgc gatcataatg acgcgtgcaa agggtgtttt ccccgttatc
    10621 ccccgccgtt gtaaggctcc tcgagattgt ctgttcaatt ttgaggatgt aaaagtgtca
    10681 aatcatgaag aaaattccgg cgctgttgcc gacaacgtaa cgccgatcga tgctgccaag
    10741 ggcaaacctg ccgccgatgg ctctgacctg ccgttcatcc ggttttccgg cggctgggtg
    10801 tacctcaaga cctgcaagac tgacaagaag ggcgaaacct attactcgct gcaggctgtc
    10861 tgtaagtctc acctgcgtgt catcggcacc ggctgggtcg atggcgaggc gttccgagtt
    10921 ctggagctgg gaaaggacaa gcgccgggta atgatgccga cgcgcctgat tggctcgcga
    10981 gatggctggg cgttccttca ggggcataac gtgcaggtca ctccagttcc cgagctacgt
    11041 cgctggctcc atgagtactt gatgtccgag acggcctacg atctgcatgg ctctccgtgc
    11101 ggcgttctac ccgagtggga cgtggctgac aagcctggtt ggcgcggtgg ggcgtacctc
    11161 ctgccagacg gcgacatgat aggcgatgtt gggaacgtga tcgccgactt caaactgcga
    11221 agcaatcacg ccgacgttgg tgtgtgcggt accgtcgact cctgggtgga aaacgtaggg
    11281 aggctgctgt acggcaacga ctcgccgacc ctggcactgg gggtggcgat ggcctcacct
    11341 atgcttggag tgctcggcgt gtccgggttt ggcgtccacc tgtacgggca gtcctccggt
    11401 ggtaagacct cctcctgtta cccggctatg tcgattttcg gatctccttc aaaccgtcag
    11461 gcttcgtgga acagcacgcc gctggcgctt actctgctgg ctcgctgctg caacgacgca
    11521 ctgatgatgc tagacgaaat gaagctcctc cgggctatgg tcatggaaga ggcgatctac
    11581 ggcaccttct cgcaacgatc gcgcatgcag gggaacaagg acgggacgct gcggcctgag
    11641 tattcctggc gctgctgcgt gctctcaacg ggtgagttgg ctgctgacgt attcattcgc
    11701 atgatgaccg ggcgtgatgc tgacgcaggg gcacttgttc gcctggtgca acttccctat
    11761 cgcccgctga caaacctcca cggcttccct gacggctccg cgttcgccga ccacctaagg
    11821 gcggcatctg acgagcacta cggtgctgtt gggcgggcgt gggtggattg gctgtcgtca
    11881 aaccagactc tggcccggga aaaattcgca gaggttaagg ctcgttggaa cgggcggggt
    11941 actcatggcc agcatcagcg cgtggcggac tacttcgcgg tgatcgagac ggcgctgacg
    12001 ctgtcctcta aaacgctcaa gctcgacgtg aaagagtgcc agggcgtgct cgatcgcttc
    12061 tacgccgact ggctcgtcga tttcagtaag ggtggcaatg gctcccatga agaggcgttg
    12121 ctggttgaac ggctggaggc ggttctgtcg caacatcaac ggttcctgtc caagacggcg
    12181 aacgatcagg actacaagca gcccggcgag ctgtggggct acttccatga ccggggcgac
    12241 gcgcgcgagt atctgctatt ccctgcggtg ttcaaagagc ggatctccgg caccatcgac
    12301 gccaaggtgg cggggcgctt gcttcacact cgtgggcttc tgcgtcgtca tggtgaacac
    12361 cttacggtgt cagagcgtgc tggcggatac ccgaacaagc gccgcttcta cgcgctgtct
    12421 ctccccgtgg ttgacgatct ggacgctgag gaggtgctct gatggctaaa cctacccccg
    12481 ctgaaagtgc catgaacgtg acgcgtttta tccgtgcgca aactctcgtc atgcttgaaa
    12541 aagtggaggc gctcgacttc gacgacctta ccgctcttgc tgaaaagctc cacgacgatg
    12601 ccgaaaaact ggagcgcctt gtcaccgatc gggtgaaaaa cggctgatcg gtagcactgg
    12661 aaccagtcca ctggttccaa acgaaaaacc tctacagccc gcgccc
//
