LOCUS       NZ_CP060063             7136 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain G6/7 plasmid pSYM12, complete sequence.
ACCESSION   NZ_CP060063
VERSION     NZ_CP060063.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15693149
            Assembly: GCF_014295235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Whole genome sequencing of five probiotic Escherichia coli strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2020) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: UAMS_Ecol_g67
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 791x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:41:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,453
            CDSs (total)                      :: 5,323
            Genes (coding)                    :: 5,011
            CDSs (with protein)               :: 5,011
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 312
            CDSs (without protein)            :: 312
            Pseudo Genes (ambiguous residues) :: 0 of 312
            Pseudo Genes (frameshifted)       :: 115 of 312
            Pseudo Genes (incomplete)         :: 212 of 312
            Pseudo Genes (internal stop)      :: 38 of 312
            Pseudo Genes (multiple problems)  :: 50 of 312
            Pseudo Genes (short protein)      :: 4 of 312
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060063.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G6/7"
                     /isolation_source="feces from healthy individual"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM12"
                     /geo_loc_name="Germany"
                     /collection_date="1954"
     gene            complement(150..572)
                     /locus_tag="H4P50_RS26740"
                     /old_locus_tag="H4P50_26740"
     CDS             complement(150..572)
                     /locus_tag="H4P50_RS26740"
                     /old_locus_tag="H4P50_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            694..972
                     /locus_tag="H4P50_RS26745"
                     /old_locus_tag="H4P50_26745"
     CDS             694..972
                     /locus_tag="H4P50_RS26745"
                     /old_locus_tag="H4P50_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(964..1254)
                     /locus_tag="H4P50_RS26750"
                     /old_locus_tag="H4P50_26750"
     CDS             complement(964..1254)
                     /locus_tag="H4P50_RS26750"
                     /old_locus_tag="H4P50_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024216191.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            1493..2992
                     /gene="csa"
                     /locus_tag="H4P50_RS26755"
                     /old_locus_tag="H4P50_26755"
     CDS             1493..2992
                     /gene="csa"
                     /locus_tag="H4P50_RS26755"
                     /old_locus_tag="H4P50_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="WP_104807859.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            complement(3020..3559)
                     /gene="csi"
                     /locus_tag="H4P50_RS26760"
                     /old_locus_tag="H4P50_26760"
     CDS             complement(3020..3559)
                     /gene="csi"
                     /locus_tag="H4P50_RS26760"
                     /old_locus_tag="H4P50_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="WP_104807860.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMAIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            3589..3744
                     /gene="csl"
                     /locus_tag="H4P50_RS26765"
                     /old_locus_tag="H4P50_26765"
     CDS             3589..3744
                     /gene="csl"
                     /locus_tag="H4P50_RS26765"
                     /old_locus_tag="H4P50_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            complement(4742..4933)
                     /locus_tag="H4P50_RS26770"
                     /old_locus_tag="H4P50_26770"
     CDS             complement(4742..4933)
                     /locus_tag="H4P50_RS26770"
                     /old_locus_tag="H4P50_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5001..5324
                     /locus_tag="H4P50_RS26775"
                     /old_locus_tag="H4P50_26775"
     CDS             5001..5324
                     /locus_tag="H4P50_RS26775"
                     /old_locus_tag="H4P50_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5533..>5970
                     /locus_tag="H4P50_RS27425"
                     /pseudo=""
     CDS             5533..>5970
                     /locus_tag="H4P50_RS27425"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6002..6562
                     /locus_tag="H4P50_RS26785"
                     /old_locus_tag="H4P50_26785"
     CDS             6002..6562
                     /locus_tag="H4P50_RS26785"
                     /old_locus_tag="H4P50_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            6569..6802
                     /locus_tag="H4P50_RS26790"
                     /old_locus_tag="H4P50_26790"
     CDS             6569..6802
                     /locus_tag="H4P50_RS26790"
                     /old_locus_tag="H4P50_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4570..4657
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4002..4546)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.343"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5315..6909
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgaatagc tatgcatacg gttaaaattt atcaggtgcg atcgcggcag cttttcgggt
       61 ggtttgttgc catttttacc tgtcagcagc cgtgatcgcg ctgaacgcat ttcagcggtg
      121 cgtacaatta agggattatg gtaaatccct tactgtctgc cctcgtatcc atcgagataa
      181 accgcagaaa tggtgtctgc cattagtttc atttttttca gcgaaggcat atcgctgttt
      241 gtgtattttc tgccagcaaa ggttgttgtg ctctggagtg agtgaaaaaa tttttctgag
      301 tggaattctt ttgcacgttg ctgtgccgtt tctgctgata ctccggctgc tttgtctttc
      361 atccccatat cccggaactg ctgaaagaac gggagcatca tacgtatggt gtctgctttc
      421 tgggtataga agttaggatc aaatccatca tctgaagcat aaacataatg tcgggtatgt
      481 cgttccaggc tggtctttgg ggtgcatcct gtaagagcta ttgttgctgt tatcagaggt
      541 atcagaagca gcctggtagc ggcagatctc ataaacatct ccttgcgttt ttaatcttga
      601 tactcaggga gttatatctc cgaagagtat ctcttatcat acgtcggttt actcccaatg
      661 tgatttgatg gtcaatgagt caggaataat cccgtgaaaa aatgtgtgcc agtcctgctt
      721 tttagtgttc tttctgcttc ggttgccagt catgcggaca gcaatgtttt tcaacaaaag
      781 gccgagaaga caaaaaacgg cattgttgcg gatgttaatg ccagacagaa aatgttcagt
      841 aacagactga agatacaact tcagatcgat gagttgagag cagagctggc tggcaccaga
      901 gatgcccaaa aaagagagga tttgcgtaaa aaactggagt ctctggagcg gcaaaagaaa
      961 tccttacgtt gatgggggcg tattttcccc ggtttcttcc ataacctttt taccgttcag
     1021 atatttacgg gcgaggcgaa tgcgtcttgc ctggtatacc gccgagtaag cctgggtggg
     1081 attcacacca ttactgattt cactgaccac atgacgctga tcagccagtg tcagcgtgta
     1141 aacctgtttc tggatgaggt cacgggcttt gtcatgctca tatccggcag caacaagggt
     1201 gtcgagaagt cgtttgtctg ttttgttcag tttatggcgg ggtgatttgc tcacatcgtt
     1261 ctccgttttt catgatccaa cagttttata tctcatcctg atttgctccg gtgttctgtt
     1321 tcgacgttct caatgcctgc ctccccatag cgtgatcctt atagcaaaaa tgaaaatcca
     1381 tgctcttgac atggacaata gtgagtagta ggttttgctg tacataaaac cagtggttat
     1441 atgtacagtg ttctgttttt aatttattgt tttaattatc aaagaggaat ttatggctaa
     1501 agaactaagt ggatatggcc caactgctgg tgagtctatg gggggaacag gggcgaatct
     1561 gaatcagcaa ggtggaaata ataatagtaa tagtggggtt cactggggtg gtggttcagg
     1621 ctctggtaat ggaggtcgtg aacatggaag tcagacgggg tggggatgga gtaaaaccaa
     1681 taatccggat gtgccgcctt atgttgatga taatgggcag gtgcgcatta ccatcacaaa
     1741 tggtctggta aaaacgcctg tatatggagt tcctggggct ggcggaaata gtgatgttca
     1801 ggggggctat atccctgaaa atcctaatga tgaagttgct cgtaagtggg ataaaaacaa
     1861 cttaccgcga gagattgatg tgagtatcga tggttttaaa tatcgtgtaa cgcttaatga
     1921 taatggtaga gctatcggta ttcttcgaac aggtgtccgt ccttacgtag ggagtgaaaa
     1981 agctaaggcg ggtattatgg aaaaaatcaa tcataaaact cctgaggaga tatatgaggc
     2041 gttaggtttt aataaagatg aatctcagcg gcaggaaaaa gcaaaacaac aggctgaaga
     2101 tgcgtgggat cgtttgcctc ctaatgtgag aaaatttgat gttgatgttg aacagttcca
     2161 ctatttagtg gtgcttgatg attatggtaa tgttttatct gtaactagaa cagctgttcg
     2221 tccttatgta gggagtgaaa aagctaaggc tggtattatg gataaagtcg accataagac
     2281 cccggaagag atatatgagg cattaggatt taataatgaa gagcctcaac gacaggatca
     2341 agctaaaaaa gctgcttatg atgtgtttta ttctttcagt atgaaccgtg accggattca
     2401 gagtgatgta cttaataaag ccgctgaagt tatctctgat ataggtaata aggtcggtga
     2461 ttatcttgga gatgcttata agtcgttagc aagagaaatt gctgatgatg tcaaaaattt
     2521 ccaggggaaa actatccgta gctacgatga tgcaatggcg tcattaaata aagttttgtc
     2581 taatccgggg tttaaattta acagggccga cagtgatgca ctggcgaacg tctggcgaag
     2641 tattgatgct caggatatgg caaataaact gggtaatatc agcaaggctt tcaaatttgc
     2701 cgatgtggtg atgaaggttg agaaggtccg ggagaaaagc atcgagggct atgaaaccgg
     2761 gaactgggga cctctgatgc tggaggtgga gtcctgggtg ctcagtggca tcgcttctgc
     2821 tgtggccctg ggtgtttttt cagccacatt aggggcatat gccttatctc ttggggctcc
     2881 tgctattgct gtcggtatcg taggtattct actcgcagca gttgtaggtg cattaattga
     2941 tgacaagttt gcagatgcac taaataaaga aatcattaaa cccgctcatt aataaatacc
     3001 gggtaaaagg aaggcagggt tactgccttt cttttaataa tttaaacgtg gtaactggtg
     3061 tgaatagcgt gaaatagctt atgaaaaaaa tgattaagta caggccaata taaaatagca
     3121 atagtgttgc atcattgttg gtcattagcc tggctggcct tcctgctgtc gttagctcaa
     3181 ggtttcggaa acagagtaaa tacaaaaaaa cagcacagac aaatggactt aataagcaac
     3241 ttctaagtac agcatttcgg tttgtttttt ttattagctt tctcgtttta aatgtcagga
     3301 taaataatat caaagcaagg gaaggcgctg ttttacaata aatatccata acctttgtca
     3361 ttaatgggtt gtaggcggag gttattgatg ggatgttttc agtactggta gctattgtgt
     3421 gcaagaacag attgtctgga tttgtatagt aaacggctag tatacagagt aaaggtataa
     3481 ctccgattgc cataaataaa tacaataagt tattggcttt tctattgtct atatctattg
     3541 aatgttcatc aatcatcatt gacctatagg attataataa aggtatatat gaaaaaaatt
     3601 attatttgtg ccatttcact aggaataatg ctgcttgcag catgccaagt caacaatgtc
     3661 agagatactg gaggtggggc tgtttcacca tcttccactg ttaccggagt gagtatggga
     3721 agtggtggta gtggtagcct gtaaaggatc ttcttgagat cctttacaga tcgtcgtagt
     3781 ctcttgccct gtaaacgaaa aaaccgcctg gggaggcggt ttagtcgaag gttaagtcag
     3841 ttggggactg cttaaccggg tagctggctt aagtggagca cagataccaa atgctgtcct
     3901 ttcagtgtag ccgctataag gcttcaactt caagagttct gcgtatctaa atccactttt
     3961 cagttaccaa tggctgctgc cagtggcgct ttgtcgtgtc ttaccggggt ggactcgaga
     4021 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4081 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
     4141 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4201 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4261 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4321 tggaaaaacg ccagctacgc ggcttttttt gccctttgcc tttttgctca catgttcttt
     4381 tctgccttat cccctgattc tgtggataac cgtatcgccg tttttgagcc agcagactcc
     4441 gctcgccgca gtcgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaaagcgc
     4501 ctggacgagc attttctcct tacgcatctg tgcggcattt cacacccggc atggtgcact
     4561 tttcatacaa tcctcaccga tgctacacga ttaagccagt atacactccg ctatcgctac
     4621 gtgactgggt catggctgcg ccccgacacc cgctaaaacc cgctgacgcg ccctgacggg
     4681 cttgtctgct cccggcatcc gctcacaggc cagctgtgac cgtctccggg agctgcatgt
     4741 gtcagaggtt ttcaccgtca tccccgaagt gtgcgaggca gctgcggtaa agctcgtcag
     4801 cgtggtcgtg aagcgcttcg cagatgtcag cctggtcgtc gacgtccagt tcattcagtt
     4861 tttccagcag ggtcagcgtc tggcttctga tgaagcgggc catgttaagg gcggtttttt
     4921 cctgtttagt cacagttctg tttctccatg tgtggggagt ctgtcggagg tttttaatga
     4981 taccggtgaa acgcgaaagg atgctcacga tacgggtcac ggatgaggaa catgcgcggc
     5041 tgctggcacg ttgtgaggga aaacaacttg cgtcatggat gcgtaaggtc tgcctggggg
     5101 caccgccatc gaaaacgtca ggtcttccga cactggcacc gccgctgttg cgccagttcg
     5161 cttcggtcgg caataacctg aatcagatag ccaggaagat aaacagcgga cagtggtcgg
     5221 ggcatgaccg ggttcatgtg gtggctgcac tgatggccat cggacgtgaa ctgagtgagc
     5281 ttagggatga ggtccgtaaa cagggagaac gggatgatag ttaagtttca tgccagggga
     5341 aaaggcggtg gcagtggtcc ggttgattac ctgctgggcc gggagcgtaa ccgggaaggg
     5401 gcgagagttc tgcggggtgc tcccgaagag gtacgggaac tcatcgatgc cacgccgttt
     5461 gcgaaaaaat acacctcggg ggttctgtct tttgcagagc agaccctgcc accgggagag
     5521 cgtgaaaggg tgatggagag ctttgagcgg gtactgatgc ccggtcttga aaaaaatcag
     5581 tacagcatcc tgtgggtgga gcaccaggac aaggggcgtc ttgagctgaa ttttgtcatc
     5641 ccgaatatgg agctggcgag cggaaaacgg ctccagccct attacgaccg ggcggaccgt
     5701 ccgcgaatca atgcctggca gacactggtg aatcaccatt acgggctgca cgacccgaac
     5761 gcaccggaga acaggcggac acttgtcacc ccgaataatc tgccgaaagc gaaacaggag
     5821 gccgcagaag cgattacgag gggactggag gctctttacc atgccggagc gctaaaaacg
     5881 cgtcaggatg tcactgaggc gcttacagcg gcaggttttg aggttgtgag gacaaccagg
     5941 agcagtatca gtatcgccga cccggagggc gggcgtaatc tcaggctgaa aggggcgctg
     6001 tatgagcaat cttttacaaa tggggacaga cttcgagaag aaactgaaag agcgtgccgt
     6061 ctctacagag aacatgctga acagcgagtt caggaagctc gaagcgtctg tagacaggga
     6121 actgaactta aacgggcaga aaatcagaga cgctatcagc gcacacacag cagcggtgaa
     6181 agaacaactg gagacgctga acagtgccgt gaacgcgcag ttcagcgcca cagagaacga
     6241 actggcccgg cggcaggaag agctgctctg gaagctgatc aaagggcgga tactgtaccc
     6301 gtcgctgacg gcgctttgcg tcacgggcgg gatttttctg gcgtcatggg ggctgattca
     6361 gtggcaggaa agccggattg cggcgaacat cctggctatc agggaccagg aagagacgct
     6421 ggcgaaactc cgggaaaaaa catggggtgt gacgtatcac gagggcagga acgggaaatt
     6481 tctggtgctg ccgtccgggg tgaaggggga aaacaactgg acggtggaga agaagaacgc
     6541 agtaagactg gtcagggagt aagtgagtat gacagagctg gaaatgcact tactgaacgc
     6601 cttagagcaa ttgcagcagg actatacgca gcggctgaaa gactgggaga gcgccttcac
     6661 ggatttgcag catatgttcg agctgacacg acaggagaac gggcaactga gggaacagtg
     6721 cgacgacttg agtcagcagg tgcagagatt agccgggcag gtggaccgct tgagtcggtt
     6781 atttatcacg aacagcagat aagggatgaa caggaaaggc aggcacgcct ggaacgggag
     6841 cgtgcgcggt cctggcatga agagcaggaa cgggaagcac aggtatggcg tggcccgtca
     6901 atggggatgt gacggtgagt tgttccggga ggttgccaga tggtaccgga acgcgttaaa
     6961 aacgcatccg ggcgcgattc tggcgtgtcg tttttgttgc tgtgttttgc ctctttgcca
     7021 gatcgcgctc attcctcagc ctcatcccgc gatgtctgcg tatgtcatca actgaaaaga
     7081 tggaaaaaaa acagtgtcat gaggtttacg caggcaaaac cagcgttatt cacatg
//
