LOCUS       NZ_CP060059             7136 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain G8 plasmid pSYM12, complete sequence.
ACCESSION   NZ_CP060059
VERSION     NZ_CP060059.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15693150
            Assembly: GCF_014295215.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Whole genome sequencing of five probiotic Escherichia coli strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Jenjaroenpun,P., Wongsurawat,T., Khan,N., Wassenaar,T.M.,
            Nookaew,I., Ussery,D.W. and Zimmerman,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2020) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: UAMS_Ecol_g8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 859x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:36:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,424
            CDSs (total)                      :: 5,294
            Genes (coding)                    :: 4,982
            CDSs (with protein)               :: 4,982
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 312
            CDSs (without protein)            :: 312
            Pseudo Genes (ambiguous residues) :: 0 of 312
            Pseudo Genes (frameshifted)       :: 122 of 312
            Pseudo Genes (incomplete)         :: 206 of 312
            Pseudo Genes (internal stop)      :: 41 of 312
            Pseudo Genes (multiple problems)  :: 54 of 312
            Pseudo Genes (short protein)      :: 4 of 312
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP060059.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G8"
                     /isolation_source="feces from healthy individual"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM12"
                     /geo_loc_name="Germany"
                     /collection_date="1954"
     gene            complement(542..697)
                     /gene="csl"
                     /locus_tag="H4P51_RS26585"
                     /old_locus_tag="H4P51_26585"
     CDS             complement(542..697)
                     /gene="csl"
                     /locus_tag="H4P51_RS26585"
                     /old_locus_tag="H4P51_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            727..1266
                     /gene="csi"
                     /locus_tag="H4P51_RS26590"
                     /old_locus_tag="H4P51_26590"
     CDS             727..1266
                     /gene="csi"
                     /locus_tag="H4P51_RS26590"
                     /old_locus_tag="H4P51_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="WP_104807860.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMAIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            complement(1294..2793)
                     /gene="csa"
                     /locus_tag="H4P51_RS26595"
                     /old_locus_tag="H4P51_26595"
     CDS             complement(1294..2793)
                     /gene="csa"
                     /locus_tag="H4P51_RS26595"
                     /old_locus_tag="H4P51_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="WP_104807859.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            3032..3322
                     /locus_tag="H4P51_RS26600"
                     /old_locus_tag="H4P51_26600"
     CDS             3032..3322
                     /locus_tag="H4P51_RS26600"
                     /old_locus_tag="H4P51_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024216191.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            complement(3314..3592)
                     /locus_tag="H4P51_RS26605"
                     /old_locus_tag="H4P51_26605"
     CDS             complement(3314..3592)
                     /locus_tag="H4P51_RS26605"
                     /old_locus_tag="H4P51_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            3714..4136
                     /locus_tag="H4P51_RS26610"
                     /old_locus_tag="H4P51_26610"
     CDS             3714..4136
                     /locus_tag="H4P51_RS26610"
                     /old_locus_tag="H4P51_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4620..4853)
                     /locus_tag="H4P51_RS26615"
                     /old_locus_tag="H4P51_26615"
     CDS             complement(4620..4853)
                     /locus_tag="H4P51_RS26615"
                     /old_locus_tag="H4P51_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4860..5420)
                     /locus_tag="H4P51_RS26620"
                     /old_locus_tag="H4P51_26620"
     CDS             complement(4860..5420)
                     /locus_tag="H4P51_RS26620"
                     /old_locus_tag="H4P51_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5452..5889)
                     /locus_tag="H4P51_RS27275"
                     /pseudo=""
     CDS             complement(<5452..5889)
                     /locus_tag="H4P51_RS27275"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6098..6421)
                     /locus_tag="H4P51_RS26630"
                     /old_locus_tag="H4P51_26630"
     CDS             complement(6098..6421)
                     /locus_tag="H4P51_RS26630"
                     /old_locus_tag="H4P51_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6489..6680
                     /locus_tag="H4P51_RS26635"
                     /old_locus_tag="H4P51_26635"
     CDS             6489..6680
                     /locus_tag="H4P51_RS26635"
                     /old_locus_tag="H4P51_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6766..6853)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..322
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.205"
                     /coverage="94.0"
                     /evalue="1.38e-152"
     Typing          6877..7134
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.655"
                     /coverage="99.0"
                     /evalue="5.460000000000001e-88"
     Typing          complement(4514..6108)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6481..6683)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.39e-10"
ORIGIN
        1 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
       61 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg tatctcagtt
      181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      241 gctgcgcctt atccggtaac tatcgtctcg agtccacccc ggtaagacac gacaaagcgc
      301 cactggcagc agccattggt aactgaaaag tggatttaga tacgcagaac tcttgaagtt
      361 gaagccttat agcggctaca ctgaaaggac agcatttggt atctgtgctc cacttaagcc
      421 agctacccgg ttaagcagtc cccaactgac ttaaccttcg actaaaccgc ctccccaggc
      481 ggttttttcg tttacagggc aagagactac gacgatctgt aaaggatctc aagaagatcc
      541 tttacaggct accactacca ccacttccca tactcactcc ggtaacagtg gaagatggtg
      601 aaacagcccc acctccagta tctctgacat tgttgacttg gcatgctgca agcagcatta
      661 ttcctagtga aatggcacaa ataataattt ttttcatata tacctttatt ataatcctat
      721 aggtcaatga tgattgatga acattcaata gatatagaca atagaaaagc caataactta
      781 ttgtatttat ttatggcaat cggagttata cctttactct gtatactagc cgtttactat
      841 acaaatccag acaatctgtt cttgcacaca atagctacca gtactgaaaa catcccatca
      901 ataacctccg cctacaaccc attaatgaca aaggttatgg atatttattg taaaacagcg
      961 ccttcccttg ctttgatatt atttatcctg acatttaaaa cgagaaagct aataaaaaaa
     1021 acaaaccgaa atgctgtact tagaagttgc ttattaagtc catttgtctg tgctgttttt
     1081 ttgtatttac tctgtttccg aaaccttgag ctaacgacag caggaaggcc agccaggcta
     1141 atgaccaaca atgatgcaac actattgcta ttttatattg gcctgtactt aatcattttt
     1201 ttcataagct atttcacgct attcacacca gttaccacgt ttaaattatt aaaagaaagg
     1261 cagtaaccct gccttccttt tacccggtat ttattaatga gcgggtttaa tgatttcttt
     1321 atttagtgca tctgcaaact tgtcatcaat taatgcacct acaactgctg cgagtagaat
     1381 acctacgata ccgacagcaa tagcaggagc cccaagagat aaggcatatg cccctaatgt
     1441 ggctgaaaaa acacccaggg ccacagcaga agcgatgcca ctgagcaccc aggactccac
     1501 ctccagcatc agaggtcccc agttcccggt ttcatagccc tcgatgcttt tctcccggac
     1561 cttctcaacc ttcatcacca catcggcaaa tttgaaagcc ttgctgatat tacccagttt
     1621 atttgccata tcctgagcat caatacttcg ccagacgttc gccagtgcat cactgtcggc
     1681 cctgttaaat ttaaaccccg gattagacaa aactttattt aatgacgcca ttgcatcatc
     1741 gtagctacgg atagttttcc cctggaaatt tttgacatca tcagcaattt ctcttgctaa
     1801 cgacttataa gcatctccaa gataatcacc gaccttatta cctatatcag agataacttc
     1861 agcggcttta ttaagtacat cactctgaat ccggtcacgg ttcatactga aagaataaaa
     1921 cacatcataa gcagcttttt tagcttgatc ctgtcgttga ggctcttcat tattaaatcc
     1981 taatgcctca tatatctctt ccggggtctt atggtcgact ttatccataa taccagcctt
     2041 agctttttca ctccctacat aaggacgaac agctgttcta gttacagata aaacattacc
     2101 ataatcatca agcaccacta aatagtggaa ctgttcaaca tcaacatcaa attttctcac
     2161 attaggaggc aaacgatccc acgcatcttc agcctgttgt tttgcttttt cctgccgctg
     2221 agattcatct ttattaaaac ctaacgcctc atatatctcc tcaggagttt tatgattgat
     2281 tttttccata atacccgcct tagctttttc actccctacg taaggacgga cacctgttcg
     2341 aagaataccg atagctctac cattatcatt aagcgttaca cgatatttaa aaccatcgat
     2401 actcacatca atctctcgcg gtaagttgtt tttatcccac ttacgagcaa cttcatcatt
     2461 aggattttca gggatatagc ccccctgaac atcactattt ccgccagccc caggaactcc
     2521 atatacaggc gtttttacca gaccatttgt gatggtaatg cgcacctgcc cattatcatc
     2581 aacataaggc ggcacatccg gattattggt tttactccat ccccaccccg tctgacttcc
     2641 atgttcacga cctccattac cagagcctga accaccaccc cagtgaaccc cactattact
     2701 attattattt ccaccttgct gattcagatt cgcccctgtt ccccccatag actcaccagc
     2761 agttgggcca tatccactta gttctttagc cataaattcc tctttgataa ttaaaacaat
     2821 aaattaaaaa cagaacactg tacatataac cactggtttt atgtacagca aaacctacta
     2881 ctcactattg tccatgtcaa gagcatggat tttcattttt gctataagga tcacgctatg
     2941 gggaggcagg cattgagaac gtcgaaacag aacaccggag caaatcagga tgagatataa
     3001 aactgttgga tcatgaaaaa cggagaacga tgtgagcaaa tcaccccgcc ataaactgaa
     3061 caaaacagac aaacgacttc tcgacaccct tgttgctgcc ggatatgagc atgacaaagc
     3121 ccgtgacctc atccagaaac aggtttacac gctgacactg gctgatcagc gtcatgtggt
     3181 cagtgaaatc agtaatggtg tgaatcccac ccaggcttac tcggcggtat accaggcaag
     3241 acgcattcgc ctcgcccgta aatatctgaa cggtaaaaag gttatggaag aaaccgggga
     3301 aaatacgccc ccatcaacgt aaggatttct tttgccgctc cagagactcc agttttttac
     3361 gcaaatcctc tcttttttgg gcatctctgg tgccagccag ctctgctctc aactcatcga
     3421 tctgaagttg tatcttcagt ctgttactga acattttctg tctggcatta acatccgcaa
     3481 caatgccgtt ttttgtcttc tcggcctttt gttgaaaaac attgctgtcc gcatgactgg
     3541 caaccgaagc agaaagaaca ctaaaaagca ggactggcac acattttttc acgggattat
     3601 tcctgactca ttgaccatca aatcacattg ggagtaaacc gacgtatgat aagagatact
     3661 cttcggagat ataactccct gagtatcaag attaaaaacg caaggagatg tttatgagat
     3721 ctgccgctac caggctgctt ctgatacctc tgataacagc aacaatagct cttacaggat
     3781 gcaccccaaa gaccagcctg gaacgacata cccgacatta tgtttatgct tcagatgatg
     3841 gatttgatcc taacttctat acccagaaag cagacaccat acgtatgatg ctcccgttct
     3901 ttcagcagtt ccgggatatg gggatgaaag acaaagcagc cggagtatca gcagaaacgg
     3961 cacagcaacg tgcaaaagaa ttccactcag aaaaattttt tcactcactc cagagcacaa
     4021 caacctttgc tggcagaaaa tacacaaaca gcgatatgcc ttcgctgaaa aaaatgaaac
     4081 taatggcaga caccatttct gcggtttatc tcgatggata cgagggcaga cagtaaggga
     4141 tttaccataa tcccttaatt gtacgcaccg ctgaaatgcg ttcagcgcga tcacggctgc
     4201 tgacaggtaa aaatggcaac aaaccacccg aaaagctgcc gcgatcgcac ctgataaatt
     4261 ttaaccgtat gcatagctat tcagccatgt gaataacgct ggttttgcct gcgtaaacct
     4321 catgacactg ttttttttcc atcttttcag ttgatgacat acgcagacat cgcgggatga
     4381 ggctgaggaa tgagcgcgat ctggcaaaga ggcaaaacac agcaacaaaa acgacacgcc
     4441 agaatcgcgc ccggatgcgt ttttaacgcg ttccggtacc atctggcaac ctcccggaac
     4501 aactcaccgt cacatcccca ttgacgggcc acgccatacc tgtgcttccc gttcctgctc
     4561 ttcatgccag gaccgcgcac gctcccgttc caggcgtgcc tgcctttcct gttcatccct
     4621 tatctgctgt tcgtgataaa taaccgactc aagcggtcca cctgcccggc taatctctgc
     4681 acctgctgac tcaagtcgtc gcactgttcc ctcagttgcc cgttctcctg tcgtgtcagc
     4741 tcgaacatat gctgcaaatc cgtgaaggcg ctctcccagt ctttcagccg ctgcgtatag
     4801 tcctgctgca attgctctaa ggcgttcagt aagtgcattt ccagctctgt catactcact
     4861 tactccctga ccagtcttac tgcgttcttc ttctccaccg tccagttgtt ttcccccttc
     4921 accccggacg gcagcaccag aaatttcccg ttcctgccct cgtgatacgt cacaccccat
     4981 gttttttccc ggagtttcgc cagcgtctct tcctggtccc tgatagccag gatgttcgcc
     5041 gcaatccggc tttcctgcca ctgaatcagc ccccatgacg ccagaaaaat cccgcccgtg
     5101 acgcaaagcg ccgtcagcga cgggtacagt atccgccctt tgatcagctt ccagagcagc
     5161 tcttcctgcc gccgggccag ttcgttctct gtggcgctga actgcgcgtt cacggcactg
     5221 ttcagcgtct ccagttgttc tttcaccgct gctgtgtgtg cgctgatagc gtctctgatt
     5281 ttctgcccgt ttaagttcag ttccctgtct acagacgctt cgagcttcct gaactcgctg
     5341 ttcagcatgt tctctgtaga gacggcacgc tctttcagtt tcttctcgaa gtctgtcccc
     5401 atttgtaaaa gattgctcat acagcgcccc tttcagcctg agattacgcc cgccctccgg
     5461 gtcggcgata ctgatactgc tcctggttgt cctcacaacc tcaaaacctg ccgctgtaag
     5521 cgcctcagtg acatcctgac gcgtttttag cgctccggca tggtaaagag cctccagtcc
     5581 cctcgtaatc gcttctgcgg cctcctgttt cgctttcggc agattattcg gggtgacaag
     5641 tgtccgcctg ttctccggtg cgttcgggtc gtgcagcccg taatggtgat tcaccagtgt
     5701 ctgccaggca ttgattcgcg gacggtccgc ccggtcgtaa tagggctgga gccgttttcc
     5761 gctcgccagc tccatattcg ggatgacaaa attcagctca agacgcccct tgtcctggtg
     5821 ctccacccac aggatgctgt actgattttt ttcaagaccg ggcatcagta cccgctcaaa
     5881 gctctccatc accctttcac gctctcccgg tggcagggtc tgctctgcaa aagacagaac
     5941 ccccgaggtg tattttttcg caaacggcgt ggcatcgatg agttcccgta cctcttcggg
     6001 agcaccccgc agaactctcg ccccttcccg gttacgctcc cggcccagca ggtaatcaac
     6061 cggaccactg ccaccgcctt ttcccctggc atgaaactta actatcatcc cgttctccct
     6121 gtttacggac ctcatcccta agctcactca gttcacgtcc gatggccatc agtgcagcca
     6181 ccacatgaac ccggtcatgc cccgaccact gtccgctgtt tatcttcctg gctatctgat
     6241 tcaggttatt gccgaccgaa gcgaactggc gcaacagcgg cggtgccagt gtcggaagac
     6301 ctgacgtttt cgatggcggt gcccccaggc agaccttacg catccatgac gcaagttgtt
     6361 ttccctcaca acgtgccagc agccgcgcat gttcctcatc cgtgacccgt atcgtgagca
     6421 tcctttcgcg tttcaccggt atcattaaaa acctccgaca gactccccac acatggagaa
     6481 acagaactgt gactaaacag gaaaaaaccg cccttaacat ggcccgcttc atcagaagcc
     6541 agacgctgac cctgctggaa aaactgaatg aactggacgt cgacgaccag gctgacatct
     6601 gcgaagcgct tcacgaccac gctgacgagc tttaccgcag ctgcctcgca cacttcgggg
     6661 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct ggcctgtgag
     6721 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggttttagc gggtgtcggg
     6781 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta atcgtgtagc
     6841 atcggtgagg attgtatgaa aagtgcacca tgccgggtgt gaaatgccgc acagatgcgt
     6901 aaggagaaaa tgctcgtcca ggcgcttttc cgcttcctcg ctcactgact cgctgcgctc
     6961 ggtcgttcga ctgcggcgag cggagtctgc tggctcaaaa acggcgatac ggttatccac
     7021 agaatcaggg gataaggcag aaaagaacat gtgagcaaaa aggcaaaggg caaaaaaagc
     7081 cgcgtagctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatc
//
