LOCUS       NZ_CP059950             6652 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain 268.2 plasmid p3, complete sequence.
ACCESSION   NZ_CP059950
VERSION     NZ_CP059950.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13613889
            Assembly: GCF_014170975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Moffat,J., Chalmers,G., Reid-Smith,R., Mulvey,M., Aguno,A.,
            Calvert,J., Cormier,A., Ricker,N. and Boerlin,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) Pathobiology, University of Guelph, 50 Stone
            Road E, Guelph, Ontario N1G 2W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 22.7x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:25:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,957
            CDSs (total)                      :: 4,841
            Genes (coding)                    :: 4,618
            CDSs (with protein)               :: 4,618
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 82 of 223
            Pseudo Genes (incomplete)         :: 139 of 223
            Pseudo Genes (internal stop)      :: 54 of 223
            Pseudo Genes (multiple problems)  :: 49 of 223
            Pseudo Genes (short protein)      :: 2 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059950.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="268.2"
                     /host="Turkey"
                     /db_xref="taxon:562"
                     /plasmid="p3"
                     /geo_loc_name="Canada"
                     /collection_date="2016/2017"
     gene            join(6485..6652,1..171)
                     /gene="mobC"
                     /locus_tag="H5C70_RS24400"
                     /old_locus_tag="H5C70_24400"
     CDS             join(6485..6652,1..171)
                     /gene="mobC"
                     /locus_tag="H5C70_RS24400"
                     /old_locus_tag="H5C70_24400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_251000578.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWIRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVR
                     EQGARDDS"
                     /name="mobC"
     gene            380..>811
                     /locus_tag="H5C70_RS24955"
                     /pseudo=""
     CDS             380..>811
                     /locus_tag="H5C70_RS24955"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            849..1367
                     /locus_tag="H5C70_RS24410"
                     /old_locus_tag="H5C70_24410"
     CDS             849..1367
                     /locus_tag="H5C70_RS24410"
                     /old_locus_tag="H5C70_24410"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061353297.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASAEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1374..1607
                     /locus_tag="H5C70_RS24415"
                     /old_locus_tag="H5C70_24415"
     CDS             1374..1607
                     /locus_tag="H5C70_RS24415"
                     /old_locus_tag="H5C70_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_061353296.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1638..2006
                     /locus_tag="H5C70_RS24420"
                     /old_locus_tag="H5C70_24420"
     CDS             1638..2006
                     /locus_tag="H5C70_RS24420"
                     /old_locus_tag="H5C70_24420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162826011.1"
                     /translation="MSYPWSVSASRKYRNGHWMALRWDGNQRQAKMLSACFRCIFWDNG
                     WFGAPGTRFSQTLLKTQERVKTHTGAILVCLCCCVLLLLPDRAHSPEFLFFRSGSVFCL
                     AESMKKIRFSSLWLAIPA"
                     /name="hypothetical protein"
     gene            complement(2165..2584)
                     /locus_tag="H5C70_RS24425"
                     /old_locus_tag="H5C70_24425"
     CDS             complement(2165..2584)
                     /locus_tag="H5C70_RS24425"
                     /old_locus_tag="H5C70_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2665..3102)
                     /locus_tag="H5C70_RS24430"
                     /old_locus_tag="H5C70_24430"
     CDS             complement(2665..3102)
                     /locus_tag="H5C70_RS24430"
                     /old_locus_tag="H5C70_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_170918986.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGKDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            3362..4525
                     /gene="cna"
                     /locus_tag="H5C70_RS24435"
                     /old_locus_tag="H5C70_24435"
     CDS             3362..4525
                     /gene="cna"
                     /locus_tag="H5C70_RS24435"
                     /old_locus_tag="H5C70_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="WP_047615919.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGSSAKAGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGEKYKNVA
                     KEVAGDIKNFHGRNVRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAQDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTVLGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            complement(4534..5058)
                     /gene="cni"
                     /locus_tag="H5C70_RS24440"
                     /old_locus_tag="H5C70_24440"
     CDS             complement(4534..5058)
                     /gene="cni"
                     /locus_tag="H5C70_RS24440"
                     /old_locus_tag="H5C70_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="WP_061353294.1"
                     /translation="MDIKDRNKILKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSSNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYLWFFIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            5086..5223
                     /locus_tag="H5C70_RS24445"
                     /old_locus_tag="H5C70_24445"
     CDS             5086..5223
                     /locus_tag="H5C70_RS24445"
                     /old_locus_tag="H5C70_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_024139133.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     gene            complement(6244..6435)
                     /locus_tag="H5C70_RS24450"
                     /old_locus_tag="H5C70_24450"
     CDS             complement(6244..6435)
                     /locus_tag="H5C70_RS24450"
                     /old_locus_tag="H5C70_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          6072..6156
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(5502..6058)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.341"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          162..1711
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.466"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagctggccg ccatcggaaa taacctgaat cagacagccc gtaaggtgaa cagcgggcag
       61 tggtcttccg gtgaccgggt tcaggtggtg gccgcactga tggccatcgg ggatgagctg
      121 cgccggctgc gtctggccgt cagggaacag ggggcgcggg atgatagtta aatttcatgc
      181 caggggaaaa ggtggtggca gtggtccggt tgattacctg ctggggaggg agcgtaaccg
      241 cgaaggcgca acggtgcttc agggtaatcc ggaagaagtc cgggaactca tcgatgccac
      301 gccatttgcg aagaaataca cgtccggtgt tctgtcgttc gcggagaagg agctgccgcc
      361 gggaggacgt gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa
      421 aaatcagtac agcatcctgt gggtggagca ccaggacaag ggacggcttg agctgaattt
      481 tgtcattccg aacatggagc tacagaccgg aaaacgtctc cagccgtact acgaccgggc
      541 agacaggccg agaattgatg cctggcagac gctggtgaat caccattacg ggctgcatga
      601 ccccaacgcc ccggagaacc gccggatact gactctctct gataacctgc cggaaacgaa
      661 acaggcgctt gctgagagcg tcacacgcgg gatagacgct ctttatcatg taggggagat
      721 aaaaggccgt caggatgtga ttcaggcgct cacagaggcg gggcttgaag tggtcagggt
      781 gacgcgaagc agcatcagca ttgcagaccc gaacggcggg aagaatatca ggctgaaagg
      841 agcgttttat gagcaatctt ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga
      901 gagccgaatc tacagagaaa atgctgaacg acgagttcag gaagctcggc gagtctgtca
      961 gcgaggctgt gacctcaaac gagacgaaaa tcagagacgc tatagccctg ttcacagcct
     1021 cggcagagaa atcgctggaa aaacacaggg aaggggtgaa agaggcgatg atgcagcaca
     1081 ggaaggacgt gttaaagctg gcagggaata cgggcatgat gttactgggg atagtctttc
     1141 tcctgtttac cgcgagtggc gggacgctct ggtatcttgg cggacggata cagacgaacc
     1201 tggaagaaat caggaagcag gaagagacat tgcagaaact gaacgcgaag acatggggcg
     1261 tggagtttgt gcaggacggg aacaggaaat ttctggtcct tccgtacggg aaatcagcag
     1321 aggtgattcc ctttcagggg aaagagtggg tacatctgaa ggagtgacac agaatgacag
     1381 agctggaaac acatttgctg aacgccttag agcagctgca acaggactat atgcagcggc
     1441 tgagcgaatg ggagagcgcc ttcgtggaat tgcagaagat gttttcgctt acgcaacggg
     1501 acaacgcgat gctgaacgag cgggtcatgc agttgagtca gcaggtgcag cacttgagcg
     1561 agcagacaga acgcttgagc cagttataca gcgagaacag gagataaggg acgaacggct
     1621 gatacaggaa cgcgaacatg agttatccct ggagcgtgag cgccagccgg aaatacagga
     1681 acggacactg gatggccctt cgctgggatg gtaaccagag acaggctaaa atgttgtcag
     1741 cgtgtttccg gtgcattttt tgggataatg gttggttcgg ggcaccggga acgcgcttca
     1801 gtcagacctt gctgaaaaca caggaacgcg ttaaaacgca tacgggcgcg attctggtgt
     1861 gtctttgttg ctgtgttttg ctgttattgc cagatcgcgc tcattcccct gaatttctgt
     1921 ttttccgctc cggttccgtc ttctgccttg ccgaaagtat gaaaaaaatc cgtttcagca
     1981 gtctctggct ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa
     2041 atttatcggg ggcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg
     2101 ctgccgtgat cgcaccgaac gcgtttcagc ggtgcgtaca attaagggat tatggtaaat
     2161 ccacttactg tctgccctcg tagccatcga gataaaccgc agaaatcgtg tcagccagca
     2221 gcctggattt tttcggggta aggtctttgc tgcggtaatc agtacccgca aattgagttg
     2281 tgccccggag tgagtttaaa aattcctggc tgtgaaactg ctgaatgcgt tgttgcacat
     2341 cactccgtga ttttcctgtc gctttatctt tagctcccat atcccagaac tgccgaaaaa
     2401 aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga aaagttggga tcaaaaccgt
     2461 catcagaagc atatacgtaa tgccgggtat gttgttccac gctgcccttt ggggtacagg
     2521 ctgtgagagc tactgttgct gttatcatgg cgataagaca aagtttgttt ccggtacgtc
     2581 tcatgagtgt ctccctacct taaagtattt attctcggtg agttattatc cgaagactat
     2641 tttatcatca gtctctgaca gcgttcagcg accgaacaac ctggaccaga atccccgttt
     2701 cttttccagt tccagtgcct gcctgagctg ggctatctca ttttgtagct gttcccgttc
     2761 tgcttcctgg cgtctgcgat ccatatcctg tgcctgttta tcctcaagca tcagcgtcag
     2821 gcattgtttc agctcattca gctcccggag aatgcgttct gtctgctgat catgtggatt
     2881 ttctgcatgt ccctcactgt gcctttctgg tgtctcattc tgctttaatt cgccgtatgc
     2941 ccggatcagt tcactggttt caaactcccg tcgtccgtcc ttgccggtac ggtaactgac
     3001 aaggcctgcg cgcatatcac ggtaaaactg tgaacgcgat ctgcctgtca tttttagtgc
     3061 gtcccggagt gtgtgccatg ccataaagtg acagtgtccc atagatgtct catctcatgg
     3121 tttcagtaga acataatgag gtctgagagc ggtaatgttt gtgccggttt ttgtggcatc
     3181 gggcgagaat agcgcgtggt gtgaaagact gtttttttga tcgttttcac aaaaatggaa
     3241 gtccaccgtc ttgacaggga aaatgcagcg gcgtagcttt tatactgtat ataaaaccag
     3301 tggttatatg tacagtgctt gtttttaact tattgtttta aaagtcaaag aggggtttat
     3361 tatgggtagt aatggcgcag ataacgcaca taacaatgct tttggtggag ggaaaaatcc
     3421 gggcattggt aataccagtg gcgctggaag taatggtagc gcatcaagta accgaggaaa
     3481 ttccaatgga tggtcatgga gtaataagcc tcataaaaat gatggcttcc acagtgatgg
     3541 ttcttaccat attacatttc atggggacaa taattcaaag cctaaacctg gtgggaatag
     3601 tggaaataga ggtaataatg gtgatggatc gagtgctaag gctggagaga taacaattac
     3661 acctgacaac tcgaaacctg gtcgttatat ttcgtcaaat ccagagtatt cattgttagc
     3721 aaaattaatt gatgcggaat caattaaagg tacagaggta tatacttttc acactagaaa
     3781 aggtcagtat gtcaaggtta ctgttccaga tagtaatatt gataaaatga gagttgatta
     3841 tgtgaactgg aagggaccga aatataacaa taaacttgtg aagaggtttg tgagccagtt
     3901 tttattattt aggaaggaag aaaaagaaaa aaatgaaaaa gaagccttgc taaaggctag
     3961 tgaacttgtg tctggtatgg gggataaact tggcgagtat cttggagaaa aatataaaaa
     4021 tgtagctaag gaagttgccg gtgatattaa aaactttcat ggccgtaatg ttcgtagcta
     4081 taatgaagca atggcttcac tgaataaagt gttagcaaat ccaaagatga aagtaaacaa
     4141 atctgataag gatgccattg tgaatgcctg gaaacaggtt aatgctcagg acatggctaa
     4201 taagattggt aatcttggca aggcatttaa ggttgctgat ttagctataa aggttgagaa
     4261 aattagggaa aaaagcattg agggatacaa tactggcaac tggggacctc tcctgctgga
     4321 ggttgaatca tggatcatcg ggggcgttgt tgctggagtt gctattagtt tgtttggggc
     4381 tgtgttgagt tttctcccaa tctcaggact tgctgttact gtgttggggg taataggaat
     4441 aatgacgatt agttacttgt catctttcat tgatgcaaat cgagtttcga atataaataa
     4501 cattatatct agtgttattc gatgacatga gtgtcagggg agtatacctc ccctggtaaa
     4561 tagtttatat gttcctataa agaaccaaag gtataaatat gtgaatatat atatcccaat
     4621 gtatagtgtt atatagaaga aagacaaaaa caggtcattt tttgacatta atacaaatgt
     4681 ttttgatgac aaggttagtt ctgtgtcagt tagcaagaaa cagtatatca tggatatata
     4741 aagaatagtg aaaggagtta atatctttag cgcccgcaga ggtgatgact ttggttttaa
     4801 ctctaattct ttgtgtgtga atatgaataa gctgattgca gctaaagggg ctgtatgaac
     4861 atagagattg aatgcttcgc ttaggggcgg gttgtttgat gaatgaaatc ctggtaggga
     4921 taggtatgcg atgatttttt caaggagtgg tattggtgca ttattataac tgaaaaatat
     4981 taatgcgaat ggggaaagta agagcagaag actgaatgat atttttttta atatcttatt
     5041 tctgtctttt atatccatgt tgctttcttt ggatggttgg aaattatgtg tgggaaaatt
     5101 ctattgatct tgttttttat catgacactt agtgcatgtc aggtcaatca catccgtgat
     5161 gtgaaaggag ggactgttgc tccatcatca tcatccagac taacaggagt tgaaattaag
     5221 taaaagatca aaggatcctc ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa
     5281 acgaaaaaac cgcctgggga ggcggtttag tcgaaggtta agtcagttgg ggactgctta
     5341 accgaggtaa ctggcttcag cagagcgcag ataccaaata ctgtcctttc agtgtagccg
     5401 cagttgggcc atcacttcaa gaactctgta agcatctgga taaatctcgc tctgctaatc
     5461 cggttaccag tggctgctgc cagtggcgtt aaggcgtgtc ttaccgggtt ggactcaaga
     5521 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5581 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
     5641 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5701 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5761 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg ggcggagcct
     5821 atggaaaaac gcctgctacg tggccttctt cctgttcctg gtcttttact cacatgttct
     5881 ttccggcctt atcccctgat tctgtggata actgttttac cgtttttgtg tgagtccgta
     5941 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc
     6001 gcctggacgt gcattttctc cttacgcatc tgtgcggcat ttcacacccg gcatggcgta
     6061 cttttcatac aatccgcact gatgccgcat ggttaagcca gtatacactc cgctatcgct
     6121 acgtgactgg gtcagggctt cgccccgaca cccgctaaaa cccgctgacg cgccctgacg
     6181 ggcttgtcgg ctcccggcat ccgctcacag acaagctgtg accgtctccg ggagctgcat
     6241 gtgtcagagg ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc
     6301 ggcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gttcgttgag
     6361 cttttccagc agcgttaatg tctggcttct gataaagcgc gccatgttaa gggcggtttt
     6421 ttcctgtttg gtcactgatg cctccgtgta aggggggatt tctgttcatg gggtaatgat
     6481 accgatgaaa cgcgagagga tgctcacaat acgggttact gatgatgaac atgcccggtt
     6541 acttgaacgc tgtgagggta aacaactggc agtatggatt cggcgggtct gcctggggga
     6601 gccggttgcc cgttccggga aactgccgac actggcaccg ccgttactgc gt
//
