LOCUS       NZ_CP059921             2096 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain 64.1 plasmid p4, complete sequence.
ACCESSION   NZ_CP059921
VERSION     NZ_CP059921.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13613898
            Assembly: GCF_014170675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Moffat,J., Chalmers,G., Reid-Smith,R., Mulvey,M., Aguno,A.,
            Calvert,J., Cormier,A., Ricker,N. and Boerlin,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) Pathobiology, University of Guelph, 50 Stone
            Road E, Guelph, Ontario N1G 2W1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.9x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:23:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,401
            CDSs (total)                      :: 5,286
            Genes (coding)                    :: 5,013
            CDSs (with protein)               :: 5,013
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 1 of 273
            Pseudo Genes (frameshifted)       :: 129 of 273
            Pseudo Genes (incomplete)         :: 172 of 273
            Pseudo Genes (internal stop)      :: 55 of 273
            Pseudo Genes (multiple problems)  :: 69 of 273
            Pseudo Genes (short protein)      :: 4 of 273
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059921.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="64.1"
                     /host="Turkey"
                     /db_xref="taxon:562"
                     /plasmid="p4"
                     /geo_loc_name="Canada"
                     /collection_date="2016/2017"
     gene            952..1941
                     /locus_tag="H5C78_RS26670"
                     /old_locus_tag="H5C78_26670"
     CDS             952..1941
                     /locus_tag="H5C78_RS26670"
                     /old_locus_tag="H5C78_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_024136759.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKGG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          1944..2025
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="90.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(1668..1859)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 ataagtgcgc acttcgtgag attccctgac tgccagtttg tcgattgcgc ggaacggctg
       61 cggcacgaaa acggcaaaat gatttactcg ccgtttgtgt gggttgtttt gtggggtgtc
      121 aatggggttg tggtcttttt tgtgggttaa agtgtgggtg attgtgtggg ctgtgtgttt
      181 tgagttgtgg gtgattatgt gggtcattgt gtgggctgta tgcacggcat ttttttgttc
      241 tcctaaaaca caggcacgat aagcggcgta cacaaaacgg ggcgtttcgc tccgtcgctt
      301 gtgccaaagg ggacacccct ttgcaaccct gtgcggttct acgaacctgg tcaggactgc
      361 gccccgacac cccgccaacg cctcacggaa cgcgaaaaaa ttccgcgtac cgttcaccgt
      421 tccctgattc gtcaaacaga aaatcgcggg ggcccgcgcc tccggcgtgg gttccgattt
      481 tgtgattgac gacggcacgg gaaaaacact ggggatggtg gttggtgtca cgcagtgagg
      541 ccaaccacct gcaagacggt tttttttgtt gttcacaaaa tcggggtgaa agtctgtgga
      601 tgagatgggt gtaagtgggt ggggttgggt gttttgggtt actgaaaatc ccgctcaaaa
      661 ttcaagcgaa tctcgcgcgc gatccttgta tttatactta agggataact ctaggattca
      721 aattcgcctc cagcccttgt gccgcaaggc ctgaaggggg atttgtcgga aaatggtttt
      781 ggagttattc tgtcgggagt ggtcgcatta aaagcgaccc ccgcaacgag ggcaatcgtt
      841 cgggggtgtg accacctgta ggaattcgaa ttggtgatac gactaacaca acaggtgata
      901 tggcaatgat aacacgtcag atgagccatc gcccactatc gggtggtgcg catgaaaaac
      961 cctgatctcc agaacctcac ggactattcc ccctcagatg cgccctggga cgctcacaga
     1021 agcgtttctg atgacgtggg agggatttac ctgcttgcag ccgaatatga gcgctacggg
     1081 gcgcgtatgg ccctctgtgg tggtttactg cgtttcgggt ggtctaccct caaggaaacc
     1141 ggagaaacgc gtctgaggct gcgtgaggcg catttctgcc gtgtccggca ttgtcctgtc
     1201 tgccagtgga ggcgctccct gatgtggcaa gcccgtttct accagtccct gccgcgtatc
     1261 gtggcggatt atcccgatgc ccgctggatg ttcctgactc tgaccgtgcg caactgtgcg
     1321 atcggggagc tgggcgagat gctgaaccgg atgaatgcag cttttcagcg catgaaggtt
     1381 cgcaaggagt tccggcctgt gcagggctgg atacgtacca cggaagtcac ccgcgggagc
     1441 gacggcagcg cacacccgca cttccacacc ctgatgatgg tgccgcctgg catgctaaat
     1501 gggaaaagtt acgtcaggca tgaacgctgg gttgaactct ggcgggagtg tctgcgggtc
     1561 gattatgacc cgaatgttga tgtccgggca gtgaaacctc gtaagccgaa gggcggcgaa
     1621 tcgctggcct gcgcgaccgc cgaactggtg cgcggtgcgg tggccgaaac cctgaaatat
     1681 tccacgaagc cggctgacat ggtggccgat cctgaatggt tcctcgaact gacccgccag
     1741 acgcacaagc gccgctttgt ggcgaccggc ggcgcactca aagacgtgct gaaactcgat
     1801 caggaaactg acgccgacat ggtgatcggt gatgatattt ccgagggcga cgatgacggt
     1861 tcacgaatcg cgttcgagtg gaaaaccgag gtcagaaagt accgtcgctc accgacgaaa
     1921 gataaaactg aatcaggcta gcggggtgtc ggggtgcacc cctgaccaga tggcaattgt
     1981 gatagcgtcg cgtgtgacgg tattacaatt acacatcctg tcccgaaaat tcccgccgtt
     2041 tctcggtttt ttgagcgtcg cagaccgcac gacaaacgaa cctaaggtga gtttgt
//
