LOCUS       NZ_CP059892            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP18069 plasmid
            pKP18069-3, complete sequence.
ACCESSION   NZ_CP059892
VERSION     NZ_CP059892.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15694293
            Assembly: GCF_014123345.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Huang,J.
  TITLE     Genome sequence of Klebsiella pneumoniae strain KP18069
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2020) Clinical Laboratory, Lishui Hospital,
            Zhejiang University School of Medicine, No.289 Kuocang Road, Lishui,
            Zhejiang 323000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.11.1; HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:25:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,530
            CDSs (total)                      :: 5,408
            Genes (coding)                    :: 5,261
            CDSs (with protein)               :: 5,261
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 61 of 147
            Pseudo Genes (incomplete)         :: 89 of 147
            Pseudo Genes (internal stop)      :: 23 of 147
            Pseudo Genes (multiple problems)  :: 23 of 147
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18069"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pKP18069-3"
     gene            1..429
                     /locus_tag="H4N62_RS27485"
                     /old_locus_tag="H4N62_27485"
     CDS             1..429
                     /locus_tag="H4N62_RS27485"
                     /old_locus_tag="H4N62_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            455..634
                     /locus_tag="H4N62_RS27490"
                     /old_locus_tag="H4N62_27490"
     CDS             455..634
                     /locus_tag="H4N62_RS27490"
                     /old_locus_tag="H4N62_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(661..1191)
                     /locus_tag="H4N62_RS27495"
                     /old_locus_tag="H4N62_27495"
     CDS             complement(661..1191)
                     /locus_tag="H4N62_RS27495"
                     /old_locus_tag="H4N62_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1198..1929)
                     /gene="mobC"
                     /locus_tag="H4N62_RS27500"
                     /old_locus_tag="H4N62_27500"
     CDS             complement(1198..1929)
                     /gene="mobC"
                     /locus_tag="H4N62_RS27500"
                     /old_locus_tag="H4N62_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1929..3893)
                     /locus_tag="H4N62_RS27505"
                     /old_locus_tag="H4N62_27505"
     CDS             complement(1929..3893)
                     /locus_tag="H4N62_RS27505"
                     /old_locus_tag="H4N62_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5377..5526)
                     /locus_tag="H4N62_RS27510"
                     /old_locus_tag="H4N62_27510"
     CDS             complement(5377..5526)
                     /locus_tag="H4N62_RS27510"
                     /old_locus_tag="H4N62_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5611..5868)
                     /locus_tag="H4N62_RS27515"
                     /old_locus_tag="H4N62_27515"
     CDS             complement(5611..5868)
                     /locus_tag="H4N62_RS27515"
                     /old_locus_tag="H4N62_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5878..7563)
                     /locus_tag="H4N62_RS27520"
                     /old_locus_tag="H4N62_27520"
     CDS             complement(5878..7563)
                     /locus_tag="H4N62_RS27520"
                     /old_locus_tag="H4N62_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7890..8135
                     /locus_tag="H4N62_RS27525"
                     /old_locus_tag="H4N62_27525"
     CDS             7890..8135
                     /locus_tag="H4N62_RS27525"
                     /old_locus_tag="H4N62_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8409..8780
                     /gene="tolA"
                     /locus_tag="H4N62_RS27530"
                     /old_locus_tag="H4N62_27530"
     CDS             8409..8780
                     /gene="tolA"
                     /locus_tag="H4N62_RS27530"
                     /old_locus_tag="H4N62_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8777..9142
                     /locus_tag="H4N62_RS27780"
     CDS             8777..9142
                     /locus_tag="H4N62_RS27780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9317..9595
                     /locus_tag="H4N62_RS27540"
                     /old_locus_tag="H4N62_27540"
     CDS             9317..9595
                     /locus_tag="H4N62_RS27540"
                     /old_locus_tag="H4N62_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9583..9897
                     /locus_tag="H4N62_RS27545"
                     /old_locus_tag="H4N62_27545"
     CDS             9583..9897
                     /locus_tag="H4N62_RS27545"
                     /old_locus_tag="H4N62_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             878..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4696..5274
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1202..1929)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gtggcgaaaa cattgctcaa cctttcacag gcagcacaag ccgcgggcat taccagaaga
       61 acgctataca atcatgtaaa acagggaaag gttaccgtgt cacgtgacgg gaaaaacaac
      121 cctgtcgtgg atgtgtcaga actgatccgt gtatacggaa atgtgaatat ccctgagaaa
      181 caaattcccg gcatttcaca ccgtgaaaat acacaaaaaa atttcccaca ggaacagctt
      241 ctggcaatgc aaaaggaact ggccgatctc cggcaggctg tcaccctgat gctggaagat
      301 aaaacatccc gggaagaaga acgccgccag cacgatgatg agcgccggaa actccaggcc
      361 gaagtggaca gattgacaac agaactgaca cagaagaaaa aacgcttctg gtccggctgg
      421 tttagctgag aacttaccga agggaatgga gaacatgacg aagcaggaaa aaactgccct
      481 caatatggct aaattcatta aggatcagtc gctgctgttg ctggaaaagc tgaacgagct
      541 ggacatggat accccggccg acatgtgcga acggcttcac gaggatgcgg aaacactctg
      601 gcaggtgatg cgggataagc tcggggaaga ataacagcac cggccatgcc ggtgctggcg
      661 ttattttttg ccgaacggcc accaccggcg ggcgggtttg tcctccggca gttcaggcac
      721 cggcacgtta aaatagcggc tgttatattc ccgcatcagc tccgctttca gcttctcgtt
      781 caccgcgctt tccccgtgcc gctcgctgac cacctcaatc atcgcgttaa tctgctccgg
      841 tgagcacttc ccggcatcaa ccacctgcac accgttctcg atggcgaacc gctccacctt
      901 tcgatagagg taatcacaca gcttcattcc tgtgggtttg tcccgttccc attgcggatt
      961 ttcaccggtg aaaaagtacg tggctttttc gtcgctgttg agccgctcgt catacatccc
     1021 gatggatttc atcatcgcca gatgctgggc ccggtcgaca aaacagaaaa catacgcccc
     1081 ggtctgccag gcgagcacca ccatgtcttc cagatgctcg ctggttggca taatgcagcg
     1141 ctcgccgtct ttgtcttcca cgtcctcaat aaataccatg tacagcgcca taatccctca
     1201 ctccgttgtc ataaatttaa agcacgcgaa cagcctctcc cggtccagct ccaccgtttt
     1261 gcgggcctcg ccggtctgtg gattcacgat ttcatccacc gccagaatcg cccgctgtac
     1321 ccggacaagc gtcggccggt cagggcagat gtaccagatt tcatcgatgc cgttcgcccg
     1381 gcggttgaac agatacccgg acaccacttc ctgataccgc ctgcggctct tgacggtgcg
     1441 ctctacctca aatgccaccg ttctgccgtc cagctccgcc agcgcgtccg gcactttcat
     1501 ccctttcagg gccatacggt gcaggtaccg ccagccggtg gccccccggc cctccatgga
     1561 gaggcgcagg gactgcacgg ccagccggtg cggcagctgc gccgccgaca cccttgacgg
     1621 ctgaaacgga ataacgtccg tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg
     1681 cggcgtcagc ccccacacgc tgacctgacg gccgtcaacc gggtatttcg cccggaccag
     1741 cagccctttc gccaccagac tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc
     1801 cagccgtcgc gcaaccagct ccgccgtgga acacagctct tcccgcagcc agcgcagggt
     1861 cagccgtgtt ttctccgcgt tccgcgcctg ccgcttcgcg tggctgacgt tgtactgctc
     1921 cagtgtcatc agtaaagccc cgcaaaatca tcatccgctt ttggtccgcc gccgtcggcc
     1981 ggaggcaggt actccgccag cgggtccgtt ttcgccagca ccgggaaggt tttcagcgaa
     2041 atttcccggc gctcaacccg cagcgggctg ctgaacgcca gccgcgccac gcccagcccg
     2101 gtcatcaccg cgaacccgtc aggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc
     2161 gcctccttct ggatgaagct gtcgccgctg atgtgctcca cgttccccgc ctcccggctg
     2221 gcccggcggc gctccacgtc cacccggatc tccccggtct ggcccgccgc ccactgcgcg
     2281 gactcctggc tggccgcgcg atagaaccag cggatcggcg tgttatccag taccgtggtt
     2341 ttcacaaaat cagcgggcag atcctgcccg cactgaccga agtcgcccag cgactggtgc
     2401 gcgagcagca ggttgcagtc ccggctgcgc agcgtcccca gcgcgttcag cacgtacttc
     2461 gacagcagat atttaatctc atcgagcatg atgctcgcgt gcggccacct ccggaactca
     2521 tcccgcgcaa tgatgatttg cgcgcagcgg gcaaacagca tcttctgcac ccggatcacc
     2581 gcctcatcgt ccatcgagcc gatgacgtac aggcagccgc cgccgttgat tatccccgcc
     2641 acatcgatac cgctgtccgt ctgcaggacg ctcaggtcgg ccacgttctc cagcttggtg
     2701 atcagcccct tcacgtcctt tttcagggcc tccggcaacg cgtacgccgc cgccagcacc
     2761 tgctgaatgt ttgccccctg cgggaactgc tccgcaatca gcttcgccgc tttctgctcg
     2821 ctgatgcggt agtggtccgc cacgtcgccg gtctcactca ggttaaaccc ggcaaccagc
     2881 agattcttca gggcgtactg gtcgagatcg cggaacagat tcagctgcgc cggtttcccc
     2941 ggccgcagat ccagcagggt gaagtccggg cagtgcgccc ggaagacgtg cggcgcccac
     3001 gcgtcccctt tggggtcgaa gtacaccaca gcgtccccga aatcgcgtac gcagcgaatg
     3061 agcgcgttgg tggccatcgc gctcttgccg ctcccgggca ggccgaggat ctgaatattg
     3121 gtcttgcgcc aggtatcacg agggaccagc accggctggc cctgctcatc cttacccaga
     3181 aacaccgcct gctgtttccg ggcctgctcc gtggaaaaat cggccagcgc gttaaccggt
     3241 accggctccc cgaaatgcag gtggcggacg tctgtcattt ctccggtccg ctggctgctg
     3301 cgccggctct cctgattcag tttcgcgttc accgcctcgt cagcggccgc aaaatgacca
     3361 tcaagcagca ggaacgccag cagccgcgcc agaaggacgc cggcgacaag ccctccggca
     3421 caccaggcta tcccgaacag gttagccggc tgcagcaggt aataatctgc gttcgcatag
     3481 aggtccgggc gcaggacggt ttctccccag ctctcgataa acacggcgac aaccagcccc
     3541 cccagaaggg ccagaccggc agcgccggtc agccagtacg cgtagttcca gacatgtcgc
     3601 cgtgccctga ccatccgccc gggtgaatcc agcgaggcca gcaccgaaaa tggcacagtc
     3661 cggttattat cgcgcttata gcgatcggca tactcccgcc gcgccttatg gctcagcccc
     3721 acaaaacgca ttcggccggc ccagagttca gccagaccga aaaacaggcc gataaccaga
     3781 aaaaaaccgg ccagactgta atactcccgg tacagccagc tgacccacgg gtaaatctgc
     3841 cgggccagct ccgggatatg ccactgccag gcaagcgaat ccgtattaaa catgcaatct
     3901 ctctccttca gacgacgggt ttaccggcgt gatgcgcagc ccccgctgca caagccgcgg
     3961 acatgcccca aggcatggcg gcggcggtca cgtccgtggt gattgcgttc agctttcacc
     4021 gcggaggcca gcctctttcc gccccatgcg gaaaaggcgc agccccggag gaacaacgag
     4081 cacgccccag cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc cggtgttccg
     4141 gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt
     4201 gctgcttcgt caggtgcgtc cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg
     4261 acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc cggacagagc cagaaagagg
     4321 ggaaaagacc gtgcgtcggt ggcgtccagc cagcgtaagc aaggtctttt cccctgaagt
     4381 cagccacgcc ccagcgggga tgacttgcat ttctccgcaa ggagaaaaaa gcaacagaga
     4441 taggttaacg taaaatgttc agcgaaaaac cgacatggtt cacctatcct gataattgat
     4501 cgtcagggaa tagaaagact taatcagggg acaatatccc acatcagcgc gtccggtgac
     4561 cgtcttgcag gtgccgggga aattgcgcct cctcgctcac tcggtcgcta cgctccgggc
     4621 gtgagactgc ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag
     4681 caggggacta acatgtgagg caaaacggca gggccgcgcc ggtggcgttt ttccataggc
     4741 tccgccctcc tgccagagtt cacataaaca gacgcttttt cggtgcatct gtgggagccg
     4801 tgaggctcaa ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca
     4861 ccgtttccgg taggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga
     4921 tacctgtccg ccgttctccc ttgcgggaag tgtggcgctt tctcatagct cacacactgg
     4981 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga actccccgtt
     5041 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc gggaaagcac
     5101 gcaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct
     5161 aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt
     5221 ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac
     5281 cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca
     5341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag
     5401 ttcagaggag gacgatggtg ccaccgttcc gccctgaaca tcccggatat aatttgcctg
     5461 acatgcggcc agaagaaagc cagcaacaat caatataaaa agaaaaatag tctttacctt
     5521 tttcatatac tttccctgta tttaaagact ccggcattca tccctgaaca ccggaagtca
     5581 gtatttcaaa tcatccttga taattttttg ttaccagtta ccgtcccgat aatcaaaagc
     5641 tatataacaa ttatccacat caggttttat ttgattttta aaaaatggct gtagtaaagg
     5701 aacccattct ttcttcaaat caaactcacc attattaata ttatccttta aaggcattcc
     5761 caagctttca atgacagaac catcgtcacc aaagtcttta gaatactctt cccctttaaa
     5821 ctcttcagtt ttcttatcaa accatgtcaa acgtaattta agccccatat ttacctctca
     5881 aagatatttt ttaatgttcc gttttggatc tggcccttta acctgcttac ccgttttggg
     5941 atcaaatgcg cctaggtgtt cgccatcact ggcacgatac ccttcaagct caccgtgctg
     6001 ggaatcccat tcataaattt tacgtttttt atcgccatac catcgggccc gcttaccacc
     6061 accaccttgt ttaggtgttt taggatcacc tcgctttaac tctccaagtc cctttatgtc
     6121 ttctgtttta ggtactggat gataatcatg gccgtagtct ttagtcccct tgcgaggctt
     6181 gttcttttcc tcatttaact tattttctgc atccttcgct tttttctcct tttgtttccg
     6241 gctctccatc gccccggtaa gtgcagcctt atctctggat aaggattcat tcattgcgtt
     6301 taaagatgcc tgagccgctt ttaatttctc attctgattc tgaatatcct tatcaagata
     6361 gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag tcacaaaatc
     6421 atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag cagcccttgc
     6481 accggggagc gccttctctg tcgatgcaat gcggctgttt aaggtcgcaa tatttttatc
     6541 ttcggcatcc agctcagctt tcgctttatc atagtctctt ttcagcccct cttccggatg
     6601 agcggcgtcc catgcctgct ggcgacgctt ttcttcctcc tgacgctgtt ttacctgtgc
     6661 cggggtaaga acatctgtca ccgacacata aaccggcttc tgtccggtct ctttcgggaa
     6721 acgaataacg gcctcacggg agttaccacc ggctgtaaaa ccagccggac gtgaatcacc
     6781 tttttcagca acaatgcctt ttggcggggc tttcgctgtc ggaacacctt tcggtacgct
     6841 cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac gttttgtcgg
     6901 tttcgcatcc accacaggga cactcattgg ccgacctgcg acaaccgcaa tatgctgccg
     6961 ctcatccttc accacatcca caacccgttg tctgacacga accgtcgcct ggtccagtgg
     7021 taaagtactt gccggagtct ccgtcaccgc atcggccggt aatgacgtca taatttttga
     7081 catcattttc ggatcatctt ttgcaatctc agaaggcagc acaccgtaga tcgcaatccc
     7141 ccacagacca aacttaaacg gccccttcag ggcagccagc acatcagcaa cggcggccga
     7201 caacgcatcg ccggaaactg ataaagccag tccttcagcc cccggagtag ccagggcagg
     7261 cagaccgaag gccatcgcag tggccgaaga ctgtccgcca ccagagctac cgctatgatt
     7321 gccgctgtta ccaccattac caccgggttt actcccgccg ttaccaaact cccccggatt
     7381 atagttatga atatctccat tcggcgtatg cgtcgtaccc cagcccggcc cgctgtttgg
     7441 atccgtacca ccacctgatg aagggccacc tttaccagac gtaccgttca cattcccact
     7501 ggcctgacca ccattatgtc ccagacctga attacccgga cctcgaccgt ctccaccact
     7561 cataccgttt tcctctttga catttaaaac aataaattaa agccaacata ctgtacatat
     7621 acacagtata attattacct tactcctccc gttttcaggt gtcaatatat gagattagtt
     7681 ctcatcttca ggcacaaaaa agcgcccgga gccacctgta agcagatcgc gtcttttccc
     7741 gaaaatttct catggcaaca aattttatta tttaagacat tcatcacact tacatttcag
     7801 atggtataga cacatctgaa agctaactgg catgaggaga ggacctgcgg ttaaactgtt
     7861 cgggaaacct gagacgcccg gaggccacta tgacgcacct gaaactgact cccgctgaag
     7921 aaaagcgcct ggcggacgcc tttgtcgctc aggcactggc catgggcctg aaagggaaca
     7981 aaacgctgcc aaaggcggta aaacaacagg tgcgggaaag ggtgcttcgt gccattcagg
     8041 acgaacgtga agccaggcgg gcgcaggaaa tgcgtcggga gaaatacgag gctacggaaa
     8101 ccaccttcac ctggcagcca cagcgccgcc ggtaaggggt atttaccata acgtcgcatt
     8161 gcccgcaccg ctcagacggt ctgagggcgt ttccggcggc tacgggataa aacaggcaac
     8221 aaaccacccg gaaaaccgcc ggaaacgcgc ccggtaaatt gtaacccgct gcatgcctat
     8281 gcacgcaact gcatggcggg ccgtttggtg taaaaacgat acgataagcc gtgtttatgt
     8341 aaatgaacgt ggccgtaaag agtgacaaat atacatgtaa cattttttac tgacaggtca
     8401 tcaacccgat gcgtttaata ccctttatcc tgctggcatt gtgcagcccc ttactcctga
     8461 cggcctgtaa gcattccacc acccagccgc cgaaaattta tactctggat aagtcatcta
     8521 aaatcagtag ctacgctgcg gccatcaaga ccgctgttga aatgcagatg tttgaaccag
     8581 aggcctatgc gggaaaaatg tgcaccgtgc ggatatccat acaacgtgat ggcactctga
     8641 acagcgccat tgctgaagga ggagatccga aactctgtaa agcagcaata tcagccgtta
     8701 cccacgccaa aattccgccg gcaccggatg aaaatacctg gcaaatattc aaaaacgccc
     8761 ctctggattt cagaccatga aagcttttac tctgacagtg ctggcggcca tgttcctcac
     8821 tccggtacat gcgataccac taaaacccgt aaccacactg cagccaacct acccggtaca
     8881 ggccgcagca aaacgcatcg aaggtcaggt gaccgccacg tttgatattc tggacgatgg
     8941 acgagtggac aacgtgtcgc ttaccggctg tcaccttttt tttcgggaaa cgctgacggc
     9001 aatgaggcac tggctatatc ccgcagggca cccggcaatg cgacaggtta taaattttga
     9061 attcagtctg aaaaatgacg gcttacagga tccgcgacca gtcaccaccc aaaagctcaa
     9121 tgatgatggt tgccgccagt aacaggtcct ttaacccgca ggctgcaata gtcctggatg
     9181 atgagtgagc ctgtagcagc cctgattagt ggaagcgaag ctgaaaaatg ggcagcaaaa
     9241 gccgtatttc tggcaaatag cgccgccacg gattaaagta gtcatgtgta atacatatga
     9301 ctacaaaggg gcttttatgt catcagcaac atccatcagg ctcgatgaag aacttaagga
     9361 ccggctgaaa acgctggccg atgaccggca ccgctcagcg cacgcgctaa tgctggaggc
     9421 aatcaccgaa tacatcgacc gggaagagaa gcgcagccag tacctgcggg acggacaggc
     9481 cgcatggcag cactatcagg aaacgggtct gcaccttact gcggaagaag cggaagcctg
     9541 gataagtacc tggggaactg agaacgagca ggatgctccg ccatgccaca ggtaacgatt
     9601 tccgcgctgg cacagcgcga tttacaacgc cttcaggact ttctcaaaac caaaaatcgg
     9661 ctggcagcca gaaaaggagg tgaggtgatc gtccgggcta tccagcaact gaaaacattg
     9721 ccagacattg gccgcccggt gccgtttctg ccgctggaat ataaggaact ggtgatcggg
     9781 tttggcgaca gtggctatgt gatgctctac cgccacgacc gggaaatgga ccagattgtg
     9841 atcgtcaccg tcaggcatca gaaagaatcc gggtatccgg gggcagacag cctctaataa
     9901 ccgatgacgg gggcgcaatt cattgcgtgg ccgtcgccgg tttgacaagc gcagcgcgtc
     9961 agaaaaccac atcctgaacg gaaaaacggg taaaattcag cgatacattt accgtgagaa
    10021 attacacacc tttcacaccc gataaaatca ggggtataca
//
