LOCUS       NZ_CP059815             2056 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterococcus faecium strain BP5067 plasmid pBP5067_P9, complete
            sequence.
ACCESSION   NZ_CP059815
VERSION     NZ_CP059815.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675394
            Assembly: GCF_012932985.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: BP5067
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.36x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 03:47:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,890
            CDSs (total)                      :: 2,799
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 29 of 80
            Pseudo Genes (incomplete)         :: 50 of 80
            Pseudo Genes (internal stop)      :: 15 of 80
            Pseudo Genes (multiple problems)  :: 11 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059815.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BP5067"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pBP5067_P9"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2017"
                     /collected_by="CMC Vellore"
     gene            268..423
                     /locus_tag="HI834_RS14335"
                     /old_locus_tag="HI834_014335"
     CDS             268..423
                     /locus_tag="HI834_RS14335"
                     /old_locus_tag="HI834_014335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            490..1092
                     /locus_tag="HI834_RS14340"
                     /old_locus_tag="HI834_014340"
     CDS             490..1092
                     /locus_tag="HI834_RS14340"
                     /old_locus_tag="HI834_014340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1228..1485)
                     /locus_tag="HI834_RS14345"
                     /old_locus_tag="HI834_014345"
     CDS             complement(1228..1485)
                     /locus_tag="HI834_RS14345"
                     /old_locus_tag="HI834_014345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(1486..1707)
                     /locus_tag="HI834_RS14350"
                     /old_locus_tag="HI834_014350"
     CDS             complement(1486..1707)
                     /locus_tag="HI834_RS14350"
                     /old_locus_tag="HI834_014350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          complement(1151..1326)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          491..1020
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gagccctgag gtttgttttt tggttttggt gtttatgaat tgggcggagg agggggggac
       61 tacgacaccc cccttattgg tcattggtca ttggtcgata gctgtttagc cttactctcc
      121 caagggtttc agcgtttttt tttgacaaaa tcagtaatgc caagggttta aggcctatta
      181 ttcgctaatt agtatctaaa ctgttgcacc ttagttactt ttttgttact atggttttta
      241 agaaaaagtc gttggaggat acgaattatg atcagtaatg aaaaaaagag agtcgttgta
      301 acgcttacta aggaagtggc agataatctt gataaaattg cgaaacaaat gggtcttagt
      361 aagtctggtc ttattacaat ttgggttaat gctaacggaa aggaaaatga gcaaaaaaaa
      421 tagcaaaagc attagtgttg gcgcactgct tttgctgtta gactgttgtt caattaaggt
      481 aattatagca tgacgagtag aaaaactaaa gatgaacgca caaggaattg gacgtttgta
      541 gtctatcctg aatcggctcc tgaaaattgg agggagtttt tagatgagtt acatgttcct
      601 tgggttgaga gtccacttca tgataaagat gtgaatcctg acggtgaatt gaaaaagcct
      661 cattggcatg ttttgataat gtttagcggt aaaaagacgt atgatcaaat acgtgagata
      721 actcaaaagc ttcggtctcc aaacccgcaa aaatgtgcta atgcaaaagg tatggtgcga
      781 tattttgctc atatggataa tccagaaaaa tttcaatatg ctaaaagtga tattatcgct
      841 catggtggtg cagaaattgc tagttatttg tcagtaacga gtgcagaacg atatgagttg
      901 attagagaga tgatgtcgtt tgttgattct aagaatatca ctgaaatcaa agatttaatt
      961 gactatgcaa tgtcagaaag atttgatgat tggtttccgc ttctttgtga taattcagct
     1021 tatattatcg ggcaatatat aaaatcgaat cgtcatggtg gaagtgtaaa ttcaaaaata
     1081 aataaaggat aagagctgtt aatttcagct ctttttttgt gcgtgaaagt cttagtcact
     1141 atgaccaaaa ctacggacga aactatgacc aaaagtaggt actatattat gaccgaaaat
     1201 aggtccaaaa tggatttttt tgtgatatca aaaaatataa ggttgttttg taagaaaacc
     1261 agttggcaca gccgacttag ccaccttcct gtaggaaggt gctatcagtt ttcccttatg
     1321 ctctttttgt tcttcagagt ttaattccag aatatttgtg agaagtaggt caaagaggtc
     1381 ggtggtttct tcgacttttc cgccggtcaa ttcgataaaa tcattggaat atgattctag
     1441 caatttttct agttgttcaa cgttgatagt tgtttcttta ggcatttact caccttcttt
     1501 ggtggttatg actttttcta gttttttaag ggcttctggg ctgaaaagat agtttcttct
     1561 accggcagag cgatattctt ttccttcagt cagatgttct ttcgctaagc gcaaaacgta
     1621 agcttggttg taatcaagtt cttgtgcaag ttcgctggtc gtcataattt cggccaaagt
     1681 gaatttttct gattttatca ctctcattta tgctccttcc atttaacgtc cgtctatatt
     1741 acgtgagtac gtcggactta cgacataagc ttataatata agttttctgt gttgtggtca
     1801 attttatatt atcgaaatga ctagtagaaa aaatcaatgc tctaaaaagg gtctattttg
     1861 cgttttaagc gatttagcgt ctatttcgtt tagttatcgg cgcaatcgtt aaaacacgta
     1921 agagaagcga gaatggagcc tgtaaggcga ctggtagcga aacggtcgtg ttagattgca
     1981 aaagccgata gctgaacgaa atagtaagcg tggcgcctgc atatatttcg gtagaaggag
     2041 gtcggggttc ggggcg
//
