LOCUS       NZ_CP059811             4465 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterococcus faecium strain BP5067 plasmid pBP5067_P5, complete
            sequence.
ACCESSION   NZ_CP059811
VERSION     NZ_CP059811.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675394
            Assembly: GCF_012932985.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 4465)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: BP5067
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.36x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 03:47:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,890
            CDSs (total)                      :: 2,799
            Genes (coding)                    :: 2,719
            CDSs (with protein)               :: 2,719
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 29 of 80
            Pseudo Genes (incomplete)         :: 50 of 80
            Pseudo Genes (internal stop)      :: 15 of 80
            Pseudo Genes (multiple problems)  :: 11 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059811.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BP5067"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pBP5067_P5"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2017"
                     /collected_by="CMC Vellore"
     gene            complement(join(4219..4465,1..293))
                     /locus_tag="HI834_RS14245"
                     /old_locus_tag="HI834_014245"
     CDS             complement(join(4219..4465,1..293))
                     /locus_tag="HI834_RS14245"
                     /old_locus_tag="HI834_014245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(309..1232)
                     /locus_tag="HI834_RS14250"
                     /old_locus_tag="HI834_014250"
     CDS             complement(309..1232)
                     /locus_tag="HI834_RS14250"
                     /old_locus_tag="HI834_014250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            complement(2522..2956)
                     /gene="bacA"
                     /locus_tag="HI834_RS14255"
                     /old_locus_tag="HI834_014255"
     CDS             complement(2522..2956)
                     /gene="bacA"
                     /locus_tag="HI834_RS14255"
                     /old_locus_tag="HI834_014255"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(3361..3534)
                     /locus_tag="HI834_RS14260"
                     /old_locus_tag="HI834_014260"
     CDS             complement(3361..3534)
                     /locus_tag="HI834_RS14260"
                     /old_locus_tag="HI834_014260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     Typing          complement(310..1232)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttttcgat ttaactgttt ttctaggaaa gaaaggagtt ttaattcgga aacttggttg
       61 gtgtttttat ggtttttggt ttttgatttt tcgccaacta gttttcgatt taagtttgcg
      121 ttatcgacta aaaacattga ttttatagct ttttgaccgg tttcgttaat cacaaacttg
      181 tttccgtttt ttgcaaacca aagttttcga tttttgtcag aaactttttt gttaattgct
      241 gttttactaa ctcctaattc ttccgcaagt tctttgattg ttttatctgc cataaatact
      301 ccttaagctt aatcgaataa gttttgtaaa tttcctagta aatcttgttt gagtttgttt
      361 gaaagttctc gtttttctgc ctgtttttct tcagtttgtt gttgttgttt aaaatcagct
      421 tcatgagtgc ctaagggaag atttagtatt cgatctttag cgcgccattt ttcttctcgt
      481 gttaattctc cattgtgatc aatattaaat aattttatac gtaaatcttc ttgtttccct
      541 ttggaaaaat cgtcagcatg gttaatttca gctttccaag taaaacgata accgataaca
      601 ggtttacctc gacctttacc atatttttta cgaatagtta atcctttaaa taagggagtg
      661 agttcttctt taattggcct taagataaat ttgtcaatat ttgacggact attccaataa
      721 cttttaggaa tatctagtaa atcaaaaaat ttttctttgg aaaattcagt ccagccttga
      781 gttcgatatt gttttaataa tcgaaacata gtttttgaat atgtgctatc caaattagta
      841 aattgctcta gattaaatct tgtccagttc tttaattgat taaaaagtcc tttaaattct
      901 ggattaactg aaattataac aagttgttta tctcgtataa tgctatattt tgtgaaagca
      961 acaaatttag ttaaagtatt tccatcatca ttccaagcat taatacttaa taatttgtca
     1021 tacgtgctac taagatcttt tataaattgt tccccacgtt ctgtataacc gcttagtttt
     1081 ttcaaatcgt gaaatgaaaa aataatctca tctacacctt tttcacgtac acgtgaagca
     1141 attgaaaaaa ataagttcat ttcacgacta ttaaattttc tcagaggaat atgatttaat
     1201 ctattttcat attttacaat ttcatttgcc ataagcttct cctactttta taaagtatga
     1261 aataaaagta acatacttta taaaaatact caataatgtc ggaaaaccta cttgaaaagc
     1321 gtagaaaacc tacttgaaaa gcgtagaaaa cctacttgaa aagcgtagaa aacctacttg
     1381 aaaaaactct gaattgcttg ggagagtaag gacacagccg acataaaaga gatataaaaa
     1441 gagatataaa aagagatata aaaagagggg aggctaattt tcccgcctaa aaaatcactc
     1501 cttttttctc taaacaggtt cgcgaattta tcaaaactgc caattccaaa ccttgctccc
     1561 tacggttcgc taaaaaggtg tgatcgctct gctctcacat tatactgcgc agtcgcctcc
     1621 ggctcctttg ctaaaagccg ttcataagga accctttgag aagctctgag cgttcgttta
     1681 agacatttta agacagtcct agtataaatc ccttatcttg ttcttaaaac gccttacagg
     1741 cggtcagggc aagccctaac aaccctaaaa ttgtctaaat gccctctaaa attcgattta
     1801 agcactctca aacctattct tatatattta tatacttagg cgtttcaaac gtcttaaaac
     1861 gcaaattaga gcttgataaa gatatttgct cgtgtgccta gacttttaaa aaagtcggca
     1921 cacgccatga caaagctgaa ataaactgct tcagctttgt catggcggat tcgctcactt
     1981 gaaaaagtgt ttatttcgct cgattattag catatatttt ttagcttttt tgctagccaa
     2041 attttgcatt aatttgctca taagtcccac cctttcagta cgtcctacgg acgtatcgtt
     2101 cgtaatgtga aagtaccttt aaaggtactt tctacaaaac tcactaaaag ggtttggcaa
     2161 gctctatatt tgctagccaa atacctccgt atttggtgca aataaccgct tgtcaaaggg
     2221 gcgcaagctc ccccttgaat ccccctcaaa acccacgctc cacaatacaa aaagtggcta
     2281 gaaaatgcta gccacttttt gtaaaattta gtatgattta tttttagcta tttagagttt
     2341 ttatttccgt aaatacttct aaaaattgct aagataaatg ctataggaat tagataaaga
     2401 atactttttt gtaagcttat attgaaaatt agcctaaata tgaagaacat agtgattaaa
     2461 actattattg aaataattac agctaaaatc tttttattca tgagatagtt tcactttctg
     2521 attattttat tacataacga gcgtaatcat tgtattgata tgatgttgta ttgctaaagt
     2581 gagcattata tgttaattgt acaccttttc cttgatttgc agctcttaca aagacattca
     2641 ttgcatcaac agcactacta aaagcatttg taataaccca tgttccaact ccaccaatag
     2701 gaataaaaga tgcaatttga gcaggagtct ttagcttatt ataatttttt ttcaaatctc
     2761 tacacatttt attagtaaca taatagttca ttttatagca agtaaatcca cgatgattat
     2821 gattatatcg tgaacttgca gcttccactt ttatacctct agcactaaaa ggaaacagat
     2881 ttaacggagt acttgctcca acaatcatac cgaacaaaaa tagacaagta ataattttaa
     2941 taattttatt tttcattgtt acttcctcct tagatataaa atcctaggaa agcgctttct
     3001 gtatatattt agaataatca gaaaattaaa tattgtcaag agggtttatt ttataaaacg
     3061 tgttatttta aaggttagta gacataatgt gtactatgcc aagtagcctc tctaccaagg
     3121 gatacagggg ctcactttgt gattttcgat tggtctaagt caaaagctct ttttaaggct
     3181 tgattttctg cagttgcttc tttccgtttc tttgtgaatt tttaccagtc attttttact
     3241 ggggtgttat ttttcataaa atatgataaa tttttggtag cgcttcataa gcgcgttgct
     3301 ttttcaagaa taaacactca ttatttttta actggctgct cgaaagagca gtctttttta
     3361 ttatttatcc gtctctttta caaacaattc ttcgttagga ctgttgtaat aatttgaatt
     3421 taatttaagt tctccgttgg aattgttttt tccaattacg gataatagca tggggctaaa
     3481 acctaatatt cctagagaaa tagaacccag taccaacaca gtttttttta acataggtta
     3541 tacctccact ttttttaacg ctaccacttg tatacacatt ttaaatacta gccttcagtt
     3601 tatcaaatgt ttaaattgaa cggatataaa tcaaaattaa attttcaaaa gttgttctaa
     3661 aaaatttttc ttttttggaa gttactctaa ctttttttgc ttgtggtgct tggtatgttt
     3721 tcttcgccga ccgtttaggt tcttgaccat tttctcttat aagtgggatt tttaccacgt
     3781 tgagagaaaa tggtcaagaa cctgggaggc ggagaatgcc cttaatacca agcaccgcaa
     3841 gcctttccca tactttattc gatgatccca cgatgatctc ataatgattc tacaatgacc
     3901 ctacgatgat caaaattctc aaaaaaaata aacgaaattt caaacgattt ttttgaaatt
     3961 ttgttaaaat cacatttgtt tttttatgac tttttcaaca aaatcgatta gaatttattt
     4021 tgtttttcca ccaaaaattg agtgagattt caacctattt taagcaataa gtcaccatat
     4081 agtttgatag tttacaacta taaacattga ttgtttacag ttgtaaacta ttttttcttt
     4141 ctcataaaaa tttgagtgat ctccccatca ggtagtacaa ctttttcctc tactatccga
     4201 actaacttct agaaaatttt agagattggt tctattagat ttccaaattt gccaccactt
     4261 tttttcaata agtggttgtg tattgtctgc ttttttttca gaaaaggcag ttgattcggt
     4321 tgttggagtt tcttctgtat aagttaaatg gagctgttct tgtaggttct caatttgttg
     4381 gttcgcttgt agagtgagtt gttgttgctg gtcgagtaat ttttgcattt gttttatttg
     4441 attatctttt tcttgtagct gtata
//
