LOCUS       NZ_CP059800            11638 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterococcus faecium strain BP3378 plasmid pBP3378_P7, complete
            sequence.
ACCESSION   NZ_CP059800
VERSION     NZ_CP059800.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675393
            Assembly: GCF_012933055.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Veeraraghavan,B.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Veeraraghavan,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 11638)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: BP3378
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 42.82x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 03:40:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,196
            CDSs (total)                      :: 3,103
            Genes (coding)                    :: 2,981
            CDSs (with protein)               :: 2,981
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 40 of 122
            Pseudo Genes (incomplete)         :: 74 of 122
            Pseudo Genes (internal stop)      :: 28 of 122
            Pseudo Genes (multiple problems)  :: 17 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059800.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BP3378"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pBP3378_P7"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            1..933
                     /locus_tag="HI838_RS15680"
                     /old_locus_tag="HI838_015680"
     CDS             1..933
                     /locus_tag="HI838_RS15680"
                     /old_locus_tag="HI838_015680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="HI838_RS15685"
                     /old_locus_tag="HI838_015685"
     CDS             950..1420
                     /locus_tag="HI838_RS15685"
                     /old_locus_tag="HI838_015685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1956
                     /locus_tag="HI838_RS15690"
                     /old_locus_tag="HI838_015690"
     CDS             1504..1956
                     /locus_tag="HI838_RS15690"
                     /old_locus_tag="HI838_015690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_060806732.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="HI838_RS15695"
                     /old_locus_tag="HI838_015695"
     CDS             2083..3264
                     /locus_tag="HI838_RS15695"
                     /old_locus_tag="HI838_015695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(3493..4641)
                     /gene="tnpB"
                     /locus_tag="HI838_RS15700"
                     /old_locus_tag="HI838_015700"
     CDS             complement(3493..4641)
                     /gene="tnpB"
                     /locus_tag="HI838_RS15700"
                     /old_locus_tag="HI838_015700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4658..5062)
                     /gene="tnpA"
                     /locus_tag="HI838_RS15705"
                     /old_locus_tag="HI838_015705"
     CDS             complement(4658..5062)
                     /gene="tnpA"
                     /locus_tag="HI838_RS15705"
                     /old_locus_tag="HI838_015705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            <5211..5327
                     /locus_tag="HI838_RS16205"
                     /pseudo=""
     CDS             <5211..5327
                     /locus_tag="HI838_RS16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /name="bacteriocin"
     gene            6133..6306
                     /locus_tag="HI838_RS15710"
                     /old_locus_tag="HI838_015710"
     CDS             6133..6306
                     /locus_tag="HI838_RS15710"
                     /old_locus_tag="HI838_015710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162897362.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKQTDK"
                     /name="hypothetical protein"
     gene            6764..7864
                     /locus_tag="HI838_RS15715"
                     /old_locus_tag="HI838_015715"
     CDS             6764..7864
                     /locus_tag="HI838_RS15715"
                     /old_locus_tag="HI838_015715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_211182505.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            7837..8280
                     /locus_tag="HI838_RS15720"
                     /old_locus_tag="HI838_015720"
     CDS             7837..8280
                     /locus_tag="HI838_RS15720"
                     /old_locus_tag="HI838_015720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            8582..8962
                     /locus_tag="HI838_RS15725"
                     /old_locus_tag="HI838_015725"
     CDS             8582..8962
                     /locus_tag="HI838_RS15725"
                     /old_locus_tag="HI838_015725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002298770.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8944..9858
                     /locus_tag="HI838_RS15730"
                     /old_locus_tag="HI838_015730"
     CDS             8944..9858
                     /locus_tag="HI838_RS15730"
                     /old_locus_tag="HI838_015730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9818..10096
                     /locus_tag="HI838_RS16210"
     CDS             9818..10096
                     /locus_tag="HI838_RS16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            10098..10787
                     /locus_tag="HI838_RS15735"
                     /old_locus_tag="HI838_015735"
     CDS             10098..10787
                     /locus_tag="HI838_RS15735"
                     /old_locus_tag="HI838_015735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806731.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8945..9855
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt
     2041 gtgttatttt tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgatacct taaagttaat cagtgaacta ctcaccactt agctaaacct
     3361 gacggttctt aacgcttgaa gtgggagctt cttgggaata gagcatactt gatggcgtat
     3421 ttctttacta acagcggtgt ctcttattta ccaagctatc cccgtagttc ctacggttct
     3481 tgatgattgg atctaagcca atcccataga atgtagtctt agaccttcgg tcagaatatt
     3541 gatactagca ttgatatcac gatcatgatg agtatgacaa acaggacaag tccattctcg
     3601 aatttcaaga gatttcttgc catctttatg tccgcactct gaacaaatct gactagacgg
     3661 aaaccattta tctactttga tgatttttcg tccataccag tcagccttgt attgtaattt
     3721 agtcacaaac tttgaccaag aaacatcaga aatactttta gctaatttat gatttcgcaa
     3781 catacctttt gtgtttaagt cttcaataca gataatatcg tgatttttga tcatttctgt
     3841 actcaactta ttcagaaaat cagtacgttg attcattact ttttcatgca atctagctac
     3901 tttgcgtttt tgtttttgat aatttttagc ttcaaataga ttggtacctt tatttttagc
     3961 caataaagca cgcctagaca atttgcgttg ttcacgcttt agtttctttt ccattttgga
     4021 cgtgaattta ttattatcaa ttttttgtcc atcagaaaga atcgcaaagt ccataatacc
     4081 taagtcaata ccaattgtgg aattagtttt aggtaattcg ataatttctt ctttacacaa
     4141 caaagaaata tagtatttac cgctagaatg acgtgatatt gtagcagatt tgataatccc
     4201 ttttggttgt ctatgaagct taattcttac taatgacttc aatttaggaa ctttgatgaa
     4261 tttactatca atcaaagcaa ctgtaccatt ttgattattc gttgtataac tctgaacagg
     4321 atttttctta cttttgaaac gtggaaatcc aacggattta tcccgaaaaa aattcttgta
     4381 tgctttatct aaattaagtt gggcattggc taaagcaagg ctatcaattt ctttcaaaaa
     4441 cggaaattct ttcttgtatt tagctggtgt cggaaaagtc atttttttag aagaatcatt
     4501 tttaacttct tcatacgcct tttttcggtc atcaagcatt agattgtaga ccttacggac
     4561 acaaccaaaa gatttggcaa agaagatttc ttgttcctct gttggataga ttctgaattt
     4621 gtatgctttt agttgttcca taaagttcac ctactttcta tttatgattg tttccttgat
     4681 tttggatata cttcttaatc acatcaattg gtgcgccacc agttgtcaga agacaaaaac
     4741 ttttagacca gaacatttct ttccaaagaa attgtttgac tcttggaaag tcacgtttta
     4801 tcaatcttga gctggcactt ttataggcat tgatgaattt tgtcaattct gttttaggtt
     4861 gagctttgaa cataatatga acgtggtctt tatcatgatt ccactccact aaagtaatgt
     4921 gatacgattc tgaaattctt tcaaaagtag ttttggcata atcagatatt tcatcatcaa
     4981 tcacttgtct acgatatttc gttactaaaa caaggtggta ataaagaaga aatactgaat
     5041 gattatttgt atctaattcc atgttatatc aactcatttc ttgtatagac tgattgtaac
     5101 atggaacaaa ataaaaaggc aatacgcctt agttgtcggt cttcgagtta ccaacggtaa
     5161 ccctcatacc gaacggcatt catcacccac ctatagagga tgggcgactt ctgcctaatt
     5221 ctgttaaaat gttagtaaat attagaggta aacaaactat gcgtacttgt taccttatga
     5281 gttggaatgc tagtagtaga acagccaaat attattacta catttagtaa aaacaaaaca
     5341 ttctttctat acaataaaat agcgtataga gaggatgttt ttaatttgaa aaggagtggt
     5401 tatctttatg aaaaatagat catttgctat attagtatta tccgtaattt tattagatat
     5461 attgttaata acattagctt taaaaacttt aaatttagta acaagttttg tcattatacc
     5521 agtaattata ggaatgtcta tttatttcat caaaaaaaat aaacaatttt tttgaatgaa
     5581 attacgggcg aaaataatca ctcaaaatta ttaataaaaa cgaatcaact actttttctg
     5641 aagaaacata caaccgtaaa caacaattgt tgtttacggt tgtaaattat caaactatat
     5701 ggtgacttgt tgcttaaaat aggttgaaat ttcgtttatt ttttttgaga aattttaccc
     5761 acgtactacc catgtaccta tccacgtatt acccatgtac ccccccatga caaatgggcg
     5821 gaggggcttg cggcgcttgg tattaaaggc attatccgcc tcccaggttt ctgaccattt
     5881 tctctcataa gtggggtttt taccgcgttg agagaaaatg gtcagaaacc taaacgatcg
     5941 gcaaagaaaa cataccaagc gccacaagca aaaaaagtta gagtaacttc cagaaaagaa
     6001 aaatttttag aacaactttt gaaaatttga ttttgattta tatccgttca atttaaacat
     6061 ttgataaact gaaggctagt atttaaaatg tgtatacaag tggtagcgtt aaaaaaagtg
     6121 gaggtataac ctatgttaaa aaaaactgtg ttggtactgg gttctatttc tctaggaata
     6181 ttaggtttta gccccatgct attatccgta attggaaaaa acaattccaa cagagaactt
     6241 aaattaaatt caaattatta caacagtcct aacgaagaat tgtttgtaaa acaaacggat
     6301 aaataataaa aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct
     6361 tgaaaaagca cgcgcttatg aagcgctacc aaaaatttac catattttac aaaaaataac
     6421 aagtcttaaa aaaatgacag ataaaaatgc acaaagaatt agatatagat aaccgcgaaa
     6481 aatcaagctt tagaaagttg cttttagcta gaccaattaa aaatcacaaa gtgagcgtct
     6541 ctatccattg gtagaggggc tacttggcat agtgtacatt atgttaacta acgctataaa
     6601 taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg ttatcgcata
     6661 ttgtatattt taattatagg tgtataccac ttaaatttaa cacaaaggcg tgattatttt
     6721 gaaacccaaa aagaaaattt atctaggtct cctcttttcc ttgatgattg gaatttttag
     6781 tataggaatt acaaatacct atgcttcaga aaattctaat ttcaatatca actcccaacc
     6841 aattgaagaa caagcaatta attttgcgaa aactgatttt gaacactcca taacttcaat
     6901 tcttttaatg gaaaataaca ccgaaactga tattaactat actcttggtt cgccatttaa
     6961 aatgctcgat ggagaaaata cggcaaatag ttctattttc tttccagtat tttcagacgc
     7021 tgataatacg attaaatata tttataccgt caaaaaaaat acaaatggag aatattcagg
     7081 aagtatttca caatttttag caaaagaact tcaaaatcta gtagatagta atataacaga
     7141 tacaacctta ttttttcaag ataatagcat ttattattcc gataatggac aaattaaact
     7201 actttggaaa tcacctttaa gtacagaaaa aaatggtgtt tcttccttta cagaaaaaaa
     7261 tgatcttata tccgtaaata taaaaaataa aattaaaaat ttctctaaaa aacaaaattt
     7321 tcctgcgatt aaacgtgtaa aaagtgtctc ctccggagat aactttatta tgattaattg
     7381 gcgtatagca gaaacccaaa caagttttcc gtggtgtgct gcttacgtag aagctgctat
     7441 cttatgtaac aaaacagatg tacatgctac ttcagctaga gccattatgc attataccta
     7501 tcctaattta aacaacagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa
     7561 atatgcaaat tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga
     7621 ggtccaaaca caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa
     7681 ttatagtaac agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca
     7741 aacttactat gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg
     7801 accagttaca attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg
     7861 gtaaataaac ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt
     7921 tcaaaaaaac aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact
     7981 aaagatcatt cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa
     8041 tataccatct tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat
     8101 ataaatgaac aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat
     8161 aaaaaacaat tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg
     8221 acaacgaatg atgggacaga agggacagct aaaccaattg aattttttaa agctgattga
     8281 tgaatagttg tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt
     8341 tacaaaaagt ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg
     8401 ggattcaagg gggagcttgc acccctttga caagcggtta tttgcaccaa atacgtaggt
     8461 atttggctag caaatataga gcttgccaaa cccttttagt gagctttact cattgtacct
     8521 ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa gggtgggact
     8581 tatgagcgaa caaaatcaaa atttggctag cgatcattct aaaaaatata cctatcgatc
     8641 cgagccaaaa caaatcagtt ttcgagtgag cgaatctgaa tttgcaaagc tgaagcagtc
     8701 agctgaagct ttgcaaatga gtgtgccggc tttcgtaaaa gccaaggcac aaaacgcgcg
     8761 tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg atttagccaa
     8821 agctggaggg aatatcaatc aaattgccaa gtggtgcaat acacaccaac acgacgttgc
     8881 tcctgatgat gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg agctacaaaa
     8941 gatatggcaa cagttaaagt aagccgaacc acttcttgta gtcgtgcaat taattacgct
     9001 gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc caaaagtgaa
     9061 atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca cttattgatt
     9121 caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg ggcagagtat
     9181 gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga caaagaccat
     9241 attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa atttcaagct
     9301 catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg tcttgcacat
     9361 aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc tgaaataaaa
     9421 ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat cgatcaaaca
     9481 atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa aacattgggg
     9541 attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa aaagaaaaaa
     9601 gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca tgagctggac
     9661 agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc tcgagctagc
     9721 attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc tgaacaaccg
     9781 gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac agaccaccaa
     9841 caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga aacaacgtgc
     9901 ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac aagttaaaaa
     9961 actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc ttattcaacg
    10021 atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg cattttggtt
    10081 agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt gttagtattt
    10141 caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta aaaggctcta
    10201 aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata atcgtttcaa
    10261 gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt ttattaggaa
    10321 atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa gtattagcac
    10381 aaaaaataga aaaatttacc gcttgttatg aggacatgaa tgatattttt gtgaagcaag
    10441 aaaagcgttt gacggataaa aatatggaaa cactcactcg actttatcgg aatttgccag
    10501 aagaacagca aaaagagtac caagaaaaat atgaacaagt aaaaaaagac gtgcaatatg
    10561 tgaaagatac gaaagtggaa gaagcttgta gtgatttatt tggagataca attccttttt
    10621 caactagcga acaagaacga aaagaacgac aacaatcagt cacatatgaa cgctatgaaa
    10681 tcttgcttca acaagcaatt aacatacaaa atctgactaa aaaagaaaca gctttaaatt
    10741 atttaaaaag cgtcaaggaa tggttagatc agcaacagca aaattgaatt tgattgaaat
    10801 tcatgttaaa ctaaacatgc aaatgttcaa acattagata cacgaaagcc ccagagaggt
    10861 cggattctct ggggcttttt ttaggttgat gtcgccttta ctgattttac ttcttgtttc
    10921 ggttattcaa ccaataactg aatacagaga tacaaactcc tacaagaagg gggcaaataa
    10981 acagttcaaa catcagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt
    11041 tattataccc tacaaaatta aatctgtgtg gcaattttaa gtatctctta tttccgggtt
    11101 gttagggctt gccctgaccg cctgtagggc gctttaaatg ccagataaag gtttttatac
    11161 taagactgtc ttaaaacggc ttagacaagc tcttagagcc tcaaccctcc tttttgaaca
    11221 atggttttag caaaggagct ggaggcgact gcgcaggtat aatgtgagag cgtagcgatc
    11281 acaccttttt ggcgaccgta gggagcgagg ttttgaatta gcagtttaga aaagttcgcg
    11341 aacctgttta aagaaaaaat aattgttttt ttaggtggga aattttgtct ccctctttta
    11401 taatcttttt ttaaacctct ttttaaacct ctttaccgtc ggctgtgtcc ttactccccc
    11461 aagggataca aaattttata ggcatgaaat tatctgttta taggcatgaa attatctgtt
    11521 tataggcatg aaattatctg tttataggca tgaaattatc tgttttaggt attgttttta
    11581 gacataatat gtcctaaaat gatgtcaata aaatcattta gactggagta acaactaa
//
