LOCUS       NZ_CP059792             2056 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterococcus faecium strain BA17124 plasmid pBA17124_P8, complete
            sequence.
ACCESSION   NZ_CP059792
VERSION     NZ_CP059792.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675398
            Assembly: GCF_012932975.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: BA17124
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 03:29:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,905
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,728
            CDSs (with protein)               :: 2,728
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 36 of 86
            Pseudo Genes (incomplete)         :: 50 of 86
            Pseudo Genes (internal stop)      :: 18 of 86
            Pseudo Genes (multiple problems)  :: 14 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059792.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BA17124"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pBA17124_P8"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="CMC Vellore"
     gene            complement(join(1989..2056,1..190))
                     /locus_tag="HI828_RS14405"
                     /old_locus_tag="HI828_014405"
     CDS             complement(join(1989..2056,1..190))
                     /locus_tag="HI828_RS14405"
                     /old_locus_tag="HI828_014405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299941.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(191..412)
                     /locus_tag="HI828_RS14410"
                     /old_locus_tag="HI828_014410"
     CDS             complement(191..412)
                     /locus_tag="HI828_RS14410"
                     /old_locus_tag="HI828_014410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     gene            1029..1184
                     /locus_tag="HI828_RS14415"
                     /old_locus_tag="HI828_014415"
     CDS             1029..1184
                     /locus_tag="HI828_RS14415"
                     /old_locus_tag="HI828_014415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            1251..1853
                     /locus_tag="HI828_RS14420"
                     /old_locus_tag="HI828_014420"
     CDS             1251..1853
                     /locus_tag="HI828_RS14420"
                     /old_locus_tag="HI828_014420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_002323513.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     Typing          complement(1912..2056)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.43e-70"
     Typing          1252..1781
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 aaggtgctat cagttttccc ttatgctctt tttgttcttc agagtttaat tccagaatat
       61 ttgtgagaag taggtcaaag aggtcggtgg tttcttcgac ttttccgccg gtcaattcga
      121 taaaatcatt ggaatatgat tctagcaatt tttctagttg ttcaacgttg atagttgttt
      181 ctttaggcat ttactcacct tctttggtgg ttatgacttt ttctagtttt ttaagggctt
      241 ctgggctgaa aagatagttt cttctaccgg cagagcgata ttcttttcct tcagtcagat
      301 gttctttcgc taagcgcaaa acgtaagctt ggttgtaatc aagttcttgt gcaagttcgc
      361 tggtcgtcat aatttcggcc aaagtgaatt tttctgattt tatcactctc atttatgctc
      421 cttccattta acgtccgtct atattacgtg agtacgtcgg acttacgaca taagcttata
      481 atataagttt tctgtgttgt ggtcaatttt atattatcga aatgactagt agaaaaaatc
      541 aatgctctaa aaagggtcta ttttgcgttt taagcgattt agcgtctatt tcgtttagtt
      601 atcggcgcaa tcgttaaaac acgtaagaga agcgagaatg gagcctgtaa ggcgactggt
      661 agcgaaacgg tcgtgttaga ttgcaaaagc cgatagctga acgaaatagt aagcgtggcg
      721 cctgcatata tttcggtaga aggaggtcgg ggttcggggc ggagccctga ggtttgtttt
      781 ttggttttgg tgtttatgaa ttgggcggag gaggggggga ctacgacacc ccccttattg
      841 gtcattggtc attggtcgat agctgtttag ccttactctc ccaagggttt cagcgttttt
      901 ttttgacaaa atcagtaatg ccaagggttt aaggcctatt attcgctaat tagtatctaa
      961 actgttgcac cttagttact tttttgttac tatggttttt aagaaaaagt cgttggagga
     1021 tacgaattat gatcagtaat gaaaaaaaga gagtcgttgt aacgcttact aaggaagtgg
     1081 cagataatct tgataaaatt gcgaaacaaa tgggtcttag taagtctggt cttattacaa
     1141 tttgggttaa tgctaacgga aaggaaaatg agcaaaaaaa atagcaaaag cattagtgtt
     1201 ggcgcactgc ttttgctgtt agactgttgt tcaattaagg taattatagc atgacgagta
     1261 gaaaaactaa agatgaacgc acaaggaatt ggacgtttgt agtctatcct gaatcggctc
     1321 ctgaaaattg gagggagttt ttagatgagt tacatgttcc ttgggttgag agtccacttc
     1381 atgataaaga tgtgaatcct gacggtgaat tgaaaaagcc tcattggcat gttttgataa
     1441 tgtttagcgg taaaaagacg tatgatcaaa tacgtgagat aactcaaaag cttcggtctc
     1501 caaacccgca aaaatgtgct aatgcaaaag gtatggtgcg atattttgct catatggata
     1561 atccagaaaa atttcaatat gctaaaagtg atattatcgc tcatggtggt gcagaaattg
     1621 ctagttattt gtcagtaacg agtgcagaac gatatgagtt gattagagag atgatgtcgt
     1681 ttgttgattc taagaatatc actgaaatca aagatttaat tgactatgca atgtcagaaa
     1741 gatttgatga ttggtttccg cttctttgtg ataattcagc ttatattatc gggcaatata
     1801 taaaatcgaa tcgtcatggt ggaagtgtaa attcaaaaat aaataaagga taagagctgt
     1861 taatttcagc tctttttttg tgcgtgaaag tcttagtcac tatgaccaaa actacggacg
     1921 aaactatgac caaaagtagg tactatatta tgaccgaaaa taggtccaaa atggattttt
     1981 ttgtgatatc aaaaaatata aggttgtttt gtaagaaaac cagttggcac agccgactta
     2041 gccaccttcc tgtagg
//
