LOCUS       NZ_CP059788             8209 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterococcus faecium strain BA17124 plasmid pBA17124_P4, complete
            sequence.
ACCESSION   NZ_CP059788
VERSION     NZ_CP059788.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675398
            Assembly: GCF_012932975.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 8209)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: BA17124
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 03:29:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,905
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,728
            CDSs (with protein)               :: 2,728
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 36 of 86
            Pseudo Genes (incomplete)         :: 50 of 86
            Pseudo Genes (internal stop)      :: 18 of 86
            Pseudo Genes (multiple problems)  :: 14 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059788.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BA17124"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pBA17124_P4"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2018"
                     /collected_by="CMC Vellore"
     gene            99..1028
                     /locus_tag="HI828_RS14620"
                     /pseudo=""
     CDS             99..1028
                     /locus_tag="HI828_RS14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1463..1803
                     /locus_tag="HI828_RS14275"
                     /old_locus_tag="HI828_014275"
                     /pseudo=""
     CDS             1463..1803
                     /locus_tag="HI828_RS14275"
                     /old_locus_tag="HI828_014275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1807..2164
                     /locus_tag="HI828_RS14280"
                     /old_locus_tag="HI828_014280"
                     /pseudo=""
     CDS             1807..2164
                     /locus_tag="HI828_RS14280"
                     /old_locus_tag="HI828_014280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2334..3714
                     /locus_tag="HI828_RS14285"
                     /old_locus_tag="HI828_014285"
                     /pseudo=""
     CDS             2334..3714
                     /locus_tag="HI828_RS14285"
                     /old_locus_tag="HI828_014285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            4161..5135
                     /locus_tag="HI828_RS14290"
                     /old_locus_tag="HI828_014290"
     CDS             4161..5135
                     /locus_tag="HI828_RS14290"
                     /old_locus_tag="HI828_014290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_169043388.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVKTFYTPLKIRWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKYQE
                     GQGRIDFNPNKINQFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPKEIETWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHFLPIDTKHTDRIMIAGLMSDHSLWSGLTRPTKYKYRNLL
                     KQESQNDELTNHLRETFSESADDLKKELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            5132..5551
                     /locus_tag="HI828_RS14295"
                     /old_locus_tag="HI828_014295"
     CDS             5132..5551
                     /locus_tag="HI828_RS14295"
                     /old_locus_tag="HI828_014295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002368182.1"
                     /translation="MSNPVEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            5530..5869
                     /locus_tag="HI828_RS14300"
                     /old_locus_tag="HI828_014300"
                     /pseudo=""
     CDS             5530..5869
                     /locus_tag="HI828_RS14300"
                     /old_locus_tag="HI828_014300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5873..6232
                     /locus_tag="HI828_RS14305"
                     /old_locus_tag="HI828_014305"
                     /pseudo=""
     CDS             5873..6232
                     /locus_tag="HI828_RS14305"
                     /old_locus_tag="HI828_014305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            6454..>7763
                     /locus_tag="HI828_RS14695"
                     /old_locus_tag="HI828_014310"
                     /pseudo=""
     CDS             6454..>7763
                     /locus_tag="HI828_RS14695"
                     /old_locus_tag="HI828_014310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     Typing          185..1068
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.022"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          4245..5127
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.014"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2341..2744
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.16e-150"
     Typing          6665..7164
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.814"
                     /coverage="100.0"
                     /evalue="1.1199999999999998e-134"
ORIGIN
        1 tcaagactag agatttttgg ttatgaattt tttagagtta cgtgaacttt tgaaaattct
       61 ttccaccaat ctcttttttc tttgaggagg aacaaaattt gagtaaaaat ggcataatcg
      121 taaatgagtg ggaaaattta acagccctat ctaacaggcg gttaaatgta aaaacttttt
      181 ggacaccccc ttgaaaaacg gtggtcaatt gaccgaatta ccattgttgg aaaactgaaa
      241 gaaaatattt attatcatac tcaaaatgat gtattgattt tagactttga acagttaatg
      301 cgactgaatg aaaataatgg ttatttaaat ctgttggtct aaatggttgg caaattgtgg
      361 ataaacatga tgagaatatt gcttacattg aaattttgaa aaatcaagaa ggaaaaaggg
      421 cgaattgact ttaatccgaa taaaattaat caatttttag ctagttctat gaaaaatttt
      481 atccatgatt tattttcgaa ccgcattttt cacgtgcaga tattgcttgc gatataattg
      541 acgtaccaga tgatttcatt acccaatatc gggtggttga tcctgtatcg tttaaaccaa
      601 tttatggtcg gtctggaaaa gttagaaacg gcttattggg gcagtcgtgc cagcgaacgt
      661 caaattcgga tgtataacaa aaattagaac aagaaagaaa acggaaaaat tgttccaaaa
      721 gaaatcgaaa cttggtggcg tttagaattg cagttgcgac gtgggaaagc cactgattgg
      781 catgcaatgg ttcacgaaag tttggatagt tttgctagtc cacacttttt accaatagat
      841 acaaaacata cagatagaat tatgattgct ggtttaatgt cagaccattc tttatggtcg
      901 gggttaaatt agaccaacca aatacaaata tcgaaatttg ttaaaacaaa gaaagtcaga
      961 atgatgaatt gacgaatcat ttacgagaaa cattttcgga atcggcagac gatttaaaaa
     1021 agaattagac acatggcttc aaaggacttg atgtaacgga agtaggtgct gaatgagtaa
     1081 tccagtagaa aatgacctca taaaaccttg ctgatgagtt gggtatatct taaaaataca
     1141 gtgcgcaatc aactggtcaa tggttatgac cttgccttgg tataaagata atggggttac
     1201 tggcaagcta gacaagggaa ttgattggtg ttgtgcggtc aatttatcgt caaaaggtga
     1261 gattagtgac tggtgattta ccggtcaatg atgggcaaat gatttttcgt tagaaaaaaa
     1321 aatgaatttt gcgtgaacaa ttagcggaaa agataggcat attttgaatt aacggaaatg
     1381 attcgtgacc aacaaaattg actttgcttc agcaacaaaa acgatcagtt gttaccagaa
     1441 aatcaagaat taaaaatcga aaatggtagc aattctggaa gtgaggtgtg acatggataa
     1501 aaattgattt gagagaagaa acagtcgata gaattgaatt gatgtatgaa cggttagctg
     1561 attatttgaa tttgccggat gaagataatt atagtgaaat gcttgaacga attatggatg
     1621 tttctcaact cggttatcaa tgctgatcta gcaaaatctt cagttttgac taatgaattt
     1681 gttcataagc atttagcgca aacaactgat gaatcagaaa aagaattttg gttgaaatta
     1741 atgagtcatg ttatgcagtt aaaccaaatt tccgttatta attccaaaga aaaggagatg
     1801 tagtttatgg gaatgacttt agcagatatt aaagcgcaag aagaaaaatt aaaaagaaca
     1861 agcgaaactt cgccaacaga aaaaacgctt gattgagaaa gaaaatgctg caatgggcaa
     1921 ggctcttcgc aagcgttttg ggtttgaaac tttgctgatt ttgagcggtg gctatcgtcg
     1981 atttctgacg atcagcaaca cgaaaaatca gtggataatg agtggcttgc tgatgaaaat
     2041 cagcgccaag cgaacgagat tatccctgat gatctgacgg cttattcggg tgataccgaa
     2101 taagaaaagt cagatgagtg agagtaaatt tacgtcaata tttaatgacg tgaatttacg
     2161 ataagcgatt attttgcgtg aagcaaaata aagcgtaaaa ggagttgagc gtgacacgct
     2221 ttcggctcac tttttttcgt tgtcacttgt gacagcgaat accctcggag aagcatttgg
     2281 aattgtgaca gccatgtcac aattaggaaa aatgttactc tgtttagtat ttcatttaaa
     2341 aaggaacgaa gaatacaagt atttctcata ataatcgtga gttaactgat cgccagaaaa
     2401 ccaatgattg gcataagcat attgacttca cgaaatcgga tgaaaatatt tatttagagc
     2461 agacgaatct agcagataaa tatgaggaat tgtttggcga agtggtagaa aaatataatg
     2521 cgaaacaaaa aagagccgat agaaaaatcg aggactattt agcgaaagtg cgcaaggata
     2581 aaaagttgga acctcaacga gaatttattg tgcaaatagg cattttagat gatttccaga
     2641 caaatcgagc agatggttct tctactggca tatcgaaaca acaagctgaa caaaatcgag
     2701 tgattgccaa taaaattttg gttcagtatt acaaggagtt tcaaagagaa accctaattt
     2761 ggctgtttaa atgctgttat tcataatgac gaaataagtc cgcacctgca cttgaatatt
     2821 gtgccggttg ctgaaggtta taaacggggt gttcaaaagc agccgagctt taataaagca
     2881 ctggttcaac aagggataca cgctgataaa gataatggtc gagtgctttt caataatttt
     2941 agaaatcaag aagttgactc tatagaacga ttaatgaatg attacggttg gaaaagggat
     3001 attgtcggta ccaacaaaat taaagatatt cacgaataca aagaaatcat gagtgaaatt
     3061 tctgaattgc gccaaacggc tgtaaaaaga gcgtgagagc gtttctgatg agttgaggta
     3121 tatttctagg caaagagaag aaaaacgagc gtgataaggc tctgatggct tctgagagag
     3181 cgaagattga agaagagaaa aaaatatgct gaatgtaaaa aagagatggc ggtcattttc
     3241 caaaaaagat atggttcctt tagaaagttt caaaggcttc ctgatggttc gtatcattta
     3301 tctgggaatg attttgttga tttgtatcaa cgagctacga gagcaacaaa tgccaagaga
     3361 aatgctgata acatgagtga gcaatattta cagatgcttg agcaaaatac agaattaaat
     3421 gagtaaagct acattggaag aaagagcgtc atttgtctgg taaaaaacaa ttagcagatt
     3481 tacaagtgga aaataaaaag ctgaaggaag aaaatcgctc tttacgccag cgtttagagc
     3541 gattaaaaac gcatttcaaa aatcgatcac tcgtttgtct atccgttttg gtcttgcaga
     3601 aaaggatatg gggctttctg aggaaaagtg ttagagcagg agaaaacctt attttatcgt
     3661 caaaacgtct cacagcgtaa attagagcgt tctgaagatg agatcgagtg gtagtttacg
     3721 atgtgcagtc aacggtaccg gaggcggttt gtgccgagga taaggagttg acggctaaac
     3781 catagcctat taagctgaca tttgctaaaa aatggccagt ccattttttg gccaacctag
     3841 cgttagcgaa gtttgggggc gcccttttca aagggtgcgg ttttattttt tggatatttg
     3901 catagctaaa gagaagtgct attattgagt tagcaaacaa atgaactgaa aaaagagtaa
     3961 aaaaatagtc gagcaacgct accaacgttc tcgaccttta cggaaactaa tcgcactagc
     4021 tccgtaactt cttaatcatt atacgaatat ttttagattt cttcaagact agagattttt
     4081 tggtaatgaa tttttaagag ttacgtgaac ttttgaaaat tctttccaga agtctctttt
     4141 tctttgagga ggaacaaaat ttgagtaaaa atggcataat cgtaaatgag tgggaaaatt
     4201 taacagccct atctaacagg cggttaaatg taaaaacttt ttacaccccc ttgaaaatac
     4261 ggtggtcaat tgaccgaatt accattgttg gaaaactgaa agaaaatatt tattatcata
     4321 ctcaaaatga tgtattgatt ttagactttg aacagttaat gcgactgaat gaaaataatg
     4381 gttatgttaa atctgttggt ctaaatggtt ggcaaattgt ggataagcat gatgagaata
     4441 ttgcttacat tgaaattttg aaatatcaag aaggtcaagg gcgaattgac tttaatccga
     4501 ataaaattaa tcagttttta gctagctcta tgaagaattt tatccatgat ttatttttgg
     4561 agccgcattt ttcacgtgca gatattgctt gcgatataat tgacgtacca gatgatttca
     4621 ttacccaata tcgggtggtt gatcctgtat cgtttaaacc aatttatggt cggtctggca
     4681 agttagaaac tgcttattgg ggcagtcgtg ccagcgaacg tcaaattcgg atgtataaca
     4741 aaaaattaga acaagaaaga aaacggaaaa ttgttccgaa agaaatcgaa acttggtggc
     4801 gtttagaatt gcagttgcga cgtgggaaag ccactgattg gcatgcaatg gttcacgaaa
     4861 gtttggatag ttttgctagt ccacactttt taccaataga tacaaaacat acagatagaa
     4921 ttatgattgc tggtttaatg tcagaccatt ctttatggtc ggggttaact agaccaacca
     4981 aatacaaata tcgaaatttg ttaaaacaag agagtcagaa tgatgaattg acgaatcatt
     5041 tacgagaaac attttcggaa tcggcagacg atttaaaaaa agaattagac acatggcttc
     5101 aaggacttga tgtaacggaa gtaggtgctg aatgagtaat ccagtagaaa aaatgaccat
     5161 aaaaaacctt gctgatgagt tgggtatatc taaaaataca gtgcgcaatc aactggtcaa
     5221 tgcaggttat gaccttgctc ggtataaaga taatggggtt atcatgttag acaaggaatt
     5281 gattggtgtt gtgcggtcaa tttatcggtc aaaaggtggt caattgactg gtgatttacc
     5341 ggtcaatgat gcaggcaatg atttcccgtt agaaaaagaa aatgaatttt tgcgtgaaca
     5401 attagcggaa aaagataggc atatttctga attaacggaa atgattcgtg accaacaaaa
     5461 attgactttg cttcagcaac aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa
     5521 aaatcgaaaa tggtggcaat tctggaagtg aggtgtgaca tggataaaat tgatttgaga
     5581 gaagaaacag tcgatagaat tgaattgatg tatgaacggt tagctgatta tttgaatttg
     5641 ccggatgaag ataattatag tgaaatgctt gaacgaatta tggatgttct caactcggtt
     5701 atcaatgctg atcttagcaa aatcttcagt tttgactaat gaatttgttc ataagcattt
     5761 agcgcaaaca actgatgaat cagaaaaaga attttggttg aaattaatga gtcatgttat
     5821 gcagttaaac caaatttccg ttattaattc caaagaaaag gagatgtagt ttatgggaat
     5881 gactttagca gatattaaag cgcaagaaga aaaattaaaa agaacaagcg aaacttcgcc
     5941 aacagaaaaa acgcttgatt gagaaagaaa atgctgcaat gggcaaggct cttcgcaagc
     6001 gttttgggtt tgaaactttg ctgattttga gcggtggcta tcgtcgattt ctgacgatca
     6061 gcaacacgaa aaatcagtgg ataatgagtg gcttgctgat gaaaaatcag cgccaagcga
     6121 acgagattat cccgatgaat ctgacggctt attcggagtg ataccgaata agaaaagtca
     6181 gatgagtgag agtaaattta cgtcaatatt taatgacgtg aatttacgat aagcgattat
     6241 tttgcgtgaa gcaaaataaa gcgtaaaagg agttgagagc gtgacacgct ttcggctcct
     6301 ttttcgttgt cacttgtgac agcgaatacc ctcggagagc atttggaatt gtgacagcca
     6361 tgtcacaatt aggaaaaatg ttactctttg acaaagagta acagaaaggt ttacaatctt
     6421 tttattagcg acagcgcgaa ggagggtttt aacttggggt ttagtatttc atttaaaaag
     6481 gaacgaagaa tacaagtatt tctcataata atcgtgagtt aactgatcgc cagaaaacca
     6541 atgattggca taagcatatt gacttcacga aatcggatga aaatatttat ttagagcaga
     6601 cgaatctagc agataaatat gaggaattgt ttggcgaagt ggtagaaaaa tataatgcga
     6661 aacaaaaaaa gagccgatag aaaaatcgag gactatttag cgaaagtgcg caaggataaa
     6721 aagttggaac ctcaacgaga atttattgtg caaataggca ttttagatga tttccagaca
     6781 aatcgagcag atggttcttc tactggcata tcgaaacaac aagctgaaca aaatcgagtg
     6841 attgccaata aaattttggt tcagtattac aaggagtttc aagagagaaa ccctaatttg
     6901 gctgtttata atgctgttat tcataatgac gaaataagtc cgcacctgca cttgaatatt
     6961 gtgccggttg ctgaaggtta taaacagggt gttcaaaagc agccgagctt taataaagca
     7021 ctggttcaac aagggataca cgctgataaa gataatggtc gagtgctttt caataatttt
     7081 agaaatcaag aagttgactc tatagaacga ttaatgaatg attacggttg gaaagggata
     7141 ttgtcggtac caacaaaatt aaagatattc acgaatacaa agaaatcatg agtgaaattt
     7201 ctgaattgcg ccaaacggct gtaaaagagc gtgagagcgt ttctgatgag ttgaggtata
     7261 tttctaggca aaaagaaaaa acgagcgtga taaggctctg atggcttctg agaaggcgaa
     7321 aattgaagaa gagaaaaaat atgctgaatg taaaaaagag atggcggtca ttttcaaaaa
     7381 gatatggttc ctttaaaagt ttcaaaggct tcctgatggt tcgtatcatt tatctgggaa
     7441 tgattttgtt gatttgtatc aacgagctac taagcaacaa acagcaaaaa atatgctgat
     7501 aacatggcga gcaatgattt acagatgctt gagcaaaata cagaaaaaat atcgctttat
     7561 ttgtaaaaac atggcattgg aagaagaagc gtcatttttg gaaaaaaaca attgatttga
     7621 tttacggttg tggaaaataa aaagctgaag gaagaaaatc gttctttacg ccagcgttta
     7681 gagcaattaa aaacgcattt caaaaatcaa tcactcgttt gtctatccgt tttggtcttg
     7741 cagaaaagga tatggggaat ttctgaagaa ttaaaatatt agatcaagag aaaaccttat
     7801 tttatcgtca aaacgtccac agcgtaaatt agagcgttct gaagaatgag atggagtggt
     7861 agttacgatg tgcagtcaac ggtaccggag gcggtttgtg ccgaggataa ggagttgacg
     7921 gctaaaccac taatcctatg aagctgacat ttgctaaaaa atggcaagtc cattttggcc
     7981 aacttagcgt tagcgaagtt tggggcgccc ttttcaaagg gtgcggtttt atgtttttgg
     8041 atatttgcat agctaaagag aagtgctatt attgagttag caaacaaata aactgaaaaa
     8101 agagcaaaaa aaaagtcgag caacgctacc aacgttctcg acctttacgg aaactaatca
     8161 cactagctcc gtaactcaaa ttcattatac gaatatttta gaggtttct
//
