LOCUS       NZ_CP059776             7974 bp    DNA     circular CON 06-NOV-2023
DEFINITION  Enterococcus faecium strain A3895 plasmid pA3895_P4, complete
            sequence.
ACCESSION   NZ_CP059776
VERSION     NZ_CP059776.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675384
            Assembly: GCF_012933265.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 7974)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A3895
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.42x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/06/2023 04:47:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 2,970
            Genes (coding)                    :: 2,868
            CDSs (with protein)               :: 2,868
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 35 of 102
            Pseudo Genes (incomplete)         :: 68 of 102
            Pseudo Genes (internal stop)      :: 25 of 102
            Pseudo Genes (multiple problems)  :: 20 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059776.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A3895"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA3895_P4"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            complement(198..368)
                     /locus_tag="HI845_RS14920"
                     /old_locus_tag="H4J14_014915"
     CDS             complement(198..368)
                     /locus_tag="HI845_RS14920"
                     /old_locus_tag="H4J14_014915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846321.1"
                     /translation="MTRKQLLFGSILLGILGFSPMLLSVINKGNKRGELKLNPNYYNGP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(384..917)
                     /locus_tag="HI845_RS14925"
                     /old_locus_tag="H4J14_014920"
     CDS             complement(384..917)
                     /locus_tag="HI845_RS14925"
                     /old_locus_tag="H4J14_014920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_125291180.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEQLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(938..1888)
                     /locus_tag="HI845_RS14930"
                     /old_locus_tag="H4J14_014925"
     CDS             complement(938..1888)
                     /locus_tag="HI845_RS14930"
                     /old_locus_tag="H4J14_014925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            2564..2650
                     /locus_tag="HI845_RS14935"
                     /old_locus_tag="H4J14_014930"
     CDS             2564..2650
                     /locus_tag="HI845_RS14935"
                     /old_locus_tag="H4J14_014930"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2771..3460)
                     /locus_tag="HI845_RS14940"
                     /old_locus_tag="H4J14_014935"
     CDS             complement(2771..3460)
                     /locus_tag="HI845_RS14940"
                     /old_locus_tag="H4J14_014935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3467..3745)
                     /locus_tag="HI845_RS14945"
                     /old_locus_tag="H4J14_014940"
     CDS             complement(3467..3745)
                     /locus_tag="HI845_RS14945"
                     /old_locus_tag="H4J14_014940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(3705..4619)
                     /locus_tag="HI845_RS14950"
                     /old_locus_tag="H4J14_014945"
     CDS             complement(3705..4619)
                     /locus_tag="HI845_RS14950"
                     /old_locus_tag="H4J14_014945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4601..4981)
                     /locus_tag="HI845_RS14955"
                     /old_locus_tag="H4J14_014950"
     CDS             complement(4601..4981)
                     /locus_tag="HI845_RS14955"
                     /old_locus_tag="H4J14_014950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5442..5876)
                     /gene="bacA"
                     /locus_tag="HI845_RS14960"
                     /old_locus_tag="H4J14_014955"
     CDS             complement(5442..5876)
                     /gene="bacA"
                     /locus_tag="HI845_RS14960"
                     /old_locus_tag="H4J14_014955"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(6106..7254)
                     /gene="tnpB"
                     /locus_tag="HI845_RS14965"
                     /old_locus_tag="H4J14_014960"
     CDS             complement(6106..7254)
                     /gene="tnpB"
                     /locus_tag="HI845_RS14965"
                     /old_locus_tag="H4J14_014960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(7271..7675)
                     /gene="tnpA"
                     /locus_tag="HI845_RS14970"
                     /old_locus_tag="H4J14_014965"
     CDS             complement(7271..7675)
                     /gene="tnpA"
                     /locus_tag="HI845_RS14970"
                     /old_locus_tag="H4J14_014965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     Typing          complement(939..1887)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3709..4619)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagctctttt taaggcttga ttttctgccg ttgcttcttt ccgtttcttt gtgaattttt
       61 accagtcatt ttttactggg gtgttatttt tcataaaata tgataaattt ttggtagcgc
      121 ttcataagcg cgttgctttt tcaagaataa acactcatta ttttttaact ggctgctcga
      181 aagagcagtc ttttttatta tttatctgtt tcttttacaa acaattcttc attaggaccg
      241 ttgtaataat ttggattcaa ttttaattca ccacgtttat tccctttatt gatcactgac
      301 aataacatcg gactgaaacc gagaattcct agtaaaatcg aaccgaatag aagttgtttt
      361 cttgtcatga atgttccctc cagttatttc catagttgcc accatttttt ttcagtatca
      421 atttgtttgt ctattgtttc tgttgcttca gaaagagtcg ttgagtcatt ctctggagct
      481 tcttctgtat aagtcaactg tagttgttct tgcagttttt cgatttgttt gttggcttgt
      541 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctctg
      601 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
      661 gtaaccacgt ttttgcttgg tatattaaca tttttatcgt tgtcttgttc gtactcgtgc
      721 aacctatcta gcaacatttg gttgcctaag ttgcgagcaa gttgcaatac tccagaacgc
      781 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
      841 tgtttcgata ttgcttgcct actcatctta aaaagttctg acaattcctt tattgtaaag
      901 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
      961 cagataataa actttttttc tcttttttcg aaagtgcgga ctctgtttgt ttttcaagag
     1021 cttctttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     1081 gtttgatttt ttcgttaatt gttgtagcag gttcttcgta ttctttttgt ttatcagcta
     1141 aagccaatgc tttattttgt tcttctttgg ttggtgcatg tttattaaaa tcatctgctt
     1201 ttttgatttc tggtttaaac gtaaattgat agccaacgac aggttttcct cgcccttttc
     1261 cgtatttctt tttgattgat aatcctttga agattgcggt taattcctgt cgtataggct
     1321 ttataacacg attatcaacg tttgcttggt tgttccagta actttttgga atatctaata
     1381 gttcatgaaa atcctcttta gaaaaataag caaaaccttt agttcgatat tgttttagta
     1441 atcgaaacat agtctttgca tagctacttt gtaattcgtt aaattgttgg agtgaatagc
     1501 gaacccactc atctaaattg tttagtaaag gtaaagcttt ttcgtaaact tgtatgtcta
     1561 catatggatc tttagcagtt ccacgtattt taaactcact aaacataacg aatctatcta
     1621 tatcgagccc attagcactt cttttaccaa aacgtaaatc cattaattta tcataagtca
     1681 tttctaaatc attaatgaaa cgattattag cagttgcttt ataattactt aaatctttta
     1741 gttggtcaaa agtaagacgg actgttttat cgcctttttc gcgcattcta gacacaatag
     1801 agaaaaatag attcatttca actggtgtaa atttccgcaa cggaatggta tttaattcat
     1861 gatggtattt cacgatttca tttgccaaag atattcctcc ttactccttc aatggataca
     1921 gacgttataa acaacgtttt gtctgtgata atacatatta taacatacgt gagtgcaaaa
     1981 aaagagttta aaagcagaca aaatgtagtt taaaagcaga caaaatgtag tttaaaagca
     2041 gacaaaatgt agtttaaaag cagacaaaat gtagtttata aaacgctgta tccattgaag
     2101 gagtaaggac acagccgacg gtaaagaggt ttaaaaaaag gtttaaaaag agattataaa
     2161 agagggtgac aaaatttccc gcctaaaaac cacttctttt ttctaccaac aggttcgcgc
     2221 atttttcaaa actgccaatt caaaaccttg ctccctacgg tcgctaaaaa agtgtgatcg
     2281 ctatgctctc acattgtact gcgcagtcgc ctaaaggctc ctttgctaaa aacaagtcag
     2341 taacggaggt gggggaagcc tctgatagct cgtctaagtc gttttaagac agtcttgggt
     2401 aaaaaacctt atcttgttct taaagcgcct tacaggcggt cagggcaagc cctaacaacc
     2461 cgaaaataag ggatacttaa aaacaccaca cagattttat tttgtaaggt ataataactt
     2521 gtcgcctatc cgacagtctt gcgactggaa ggaggtgtat ctaatgtttg aacggttcat
     2581 ttgccccctt cttgtagaag tttgtgtctc tttattcagt tattggttga ataaccgaaa
     2641 caagaagtaa aatcaataaa ggcgacatca acctgaaaaa aagccccaga gaatccgacc
     2701 tctctggggc tttcgtgtat ctaatgtttg atcatttgca ggttcagttt aacatgattg
     2761 ctaatcaaat tcaattttgt tgttgctgat ccaaccattc cttgacactt tctagataat
     2821 ttaatgccgt ttcttttttc gttggatttt gtatgtttgt tgcttgctga agcagtgttt
     2881 cataacgttc gtatgtgacc gattgttgcc gttcttttcg ttcttgttca ctggttgaaa
     2941 aagggattgt atcgctaaat aagttacaac aagcttcttc tgtctgcgta tctttaaaat
     3001 attgcacgtc tttttttact tgctcatatt tttcttgtac ctctttttgc tgtttttctg
     3061 gtaagttttg ataaaaacga gtgagttttt tcatgttttt atctgttaaa cgtttctctt
     3121 gatccacaaa aatattgttc atgtcctcat aacaagcggt aaattcctcc attttttgtg
     3181 ctaatgcttg ctcttttttc tttttatcac atgctgcttc atacttttga gtgatatccc
     3241 ctaataaaat gcttcttgaa acacgtttat taccctctaa aacgccgaat aaaccactta
     3301 aaacgattga taaggcaaat ataaccccga ccaataaaaa tcgcttaaat cgaattttag
     3361 agccttttaa tgaatatctt gtttgataag ccttatagct aagccaaata attcctaaaa
     3421 tactggcaga aatgagtaga tagggtaaat agaatgccat gtctgtttat tctccccata
     3481 ccaaccaaaa ggctaataat gttagtacta agcaacataa gggggcaaga atatccatcg
     3541 atcgttgaat taaagccttt ttttgttctt ggtttcgctg gtctaatagc gcttggtgga
     3601 gttttttgac ttgtgaatcc atcgtagttt gtatcctttc ttgggtataa ggcgcaagaa
     3661 aggcacgttg tctctttatt tcgttttcga tgtcttttaa cgttttagag cgataattct
     3721 tgttggtggt ctgttcctcg ttcaacgtgt tgttcatggt ttcttcgagt cggtctaatt
     3781 tccgattgtt caggcgtaaa agttcgttgt tctcttcgta cttcttctgc agtttcgcga
     3841 atgctagctc gagctcttcg tagctcgttg gttcgttgtt gattgtaggt tcgttctcgt
     3901 ctgtccagct catgtaaaat cgtctccttt tcgtaatcct ttcctagttt gttggatcgt
     3961 acttttttct ttttgtggac caattcgtaa gtaaaatttt ttccgcgatt caagcaatga
     4021 atgccgaatg ttttaagtga atcacaaaat tgggtatacg ttcgagtttt tttgtttttc
     4081 attgtttgat caatcgcaaa ccgaatttcg tctttccaaa ccggttcatt tttttctttt
     4141 aatttaattt cagacatggt tcgtttttcg gttttctttc ctcgttccgt gattgttaag
     4201 tcatgttcaa gacaaatctc gtcatttagg tcataacatt tgtttaaaaa tgcttgccca
     4261 tgggcttgaa attttttccc agtttcaaga ttaacggagt taatgacaat gtgattatga
     4321 atatggtctt tgtctgtatg tgtgtaaatc gcgatttggt ggttaggggc tatttttttc
     4381 gcatactctg cccctagttg attggctttt tcagcggtaa tttctcctgg tcgaaaggac
     4441 tgaatcaata agtgtgcttg tacacgatcg tcttttccat aaagcgtgcg tatttgtttc
     4501 atttcacttt tggcataatc aatgtcacag tttattcctg ttttgacggt tgctcgtggt
     4561 tctgcgtaat tgattgctcg gctacatgaa gtggttcgac ttactttaac tgttgccata
     4621 tcttttgtag ctccttttgc atgattttta ggttttcgga caaacgttga gcatcatcag
     4681 gagcaacgtc gtgttggtgt gtattgcacc acttggcaat ttgattgata ttccctccag
     4741 ctttggctaa atcgcgcgcg attgcctggg caatgtctgg cgccactttc ggtgtcacca
     4801 gacgcgcgtt ttgtgccttg gctttcacga aagccggcac actcatttgc aaagcttcag
     4861 ctgactgctt cagctttgca aattcggatt cgctcactcg aaaactgatt tgttttggct
     4921 cggatcgata ggtatatttt ttagaatgat cgctagccaa attttgatgt tgttcgctca
     4981 taagtcccac cctttcagta cgtcctacgg acgtatcgtt cgcaatacca ttgtacctaa
     5041 aaaggtacaa tgagtaaagc tcactaaaag ggtttggcaa gctctatatt tgctagccaa
     5101 atacctccgt atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat
     5161 ccccctcaaa acccacgctc cacaatacaa aaagtggcta gcattttcta gccacttttt
     5221 gtaaaattta gtatgattta tttttagcta tttagagttt ttatttccgt aaatacttct
     5281 aaaaattgct aagataaatg ctataggaat tagataaaga atactttttt gtaagcttat
     5341 attgaaaatt agcctaaata tgaagaacat agtgattaaa actattattg aaataattac
     5401 agctaaaatc tttttattca tgagatagtt tcactttctg attattttat tacataacga
     5461 gcgtaatcat tgtattgata tgatgttgta ttgctaaagt gagcattata tgttaattgt
     5521 acaccttttc cttgatttgc agctcttaca aagacattca ttgcatcaac agcactacta
     5581 aaagcatttg taataaccca tgttccaact ccaccaatag gaataaaaga tgcaatttga
     5641 gcaggagtct ttagcttatt ataatttttt ttcaaatctc tacacatttt attagtaaca
     5701 taatagttca ttttatagca agtaaatcca cgatgattat gattatatcg tgaacttgca
     5761 gcttccactt ttatacctct agcactaaaa ggaaacagat ttaacggagt acttgctcca
     5821 acaatcatac cgaacaaaaa tagacaagta ataattttaa taattttatt tttcattgtt
     5881 acttcctcct tagatataaa atcctaggaa agcgcttcct gtatatattt agaataatca
     5941 gaaaagtgaa ctactcacca cttagctaaa cctgacggtt cttaacgctt gaagtgggag
     6001 cttcttggga atagagcata cttgatagcg tatttcttta ctaacagcgg tgtctcttat
     6061 ttaccaagct atccccgtag ttcctacggt tcttgatgat tggatctaag ccaatcccat
     6121 agaatgtagt cttagacctt cggtcagaat attgatacta gcattgatat cacgatcatg
     6181 atgagtatga caaacaggac aagtccattc tcgaatttca agagatttct tgccatcttt
     6241 atgtccgcac tctgaacaaa tctgactaga cggaaaccat ttatctactt tgatgatttt
     6301 tcgtccatac cagtcagcct tgtattgtaa tttagtcaca aactttgacc aagaaacatc
     6361 agaaatactt ttagctaatt tatgatttcg caacatacct tttgtgttta agtcttcaat
     6421 acagataata tcgtgatttt tgatcatttc tgtactcaac ttattcagaa aatcagtacg
     6481 ttgattcatt actttttcat gcaatctagc tactttgcgt ttttgttttt gataattttt
     6541 agcttcaaat agattggtac ctttattttt agccaataaa gcacgcctag acaatttgcg
     6601 ttgttcacgc tttagtttct tttccatttt ggacgtgaat ttattattat caattttttg
     6661 tccatcagaa agaatcgcaa agtccataat acctaagtca ataccaattg tggaattagt
     6721 tttaggtaat tcgataattt cttctttaca caacaaagaa atatagtatt taccgctaga
     6781 atgacgtgat attgtagcag atttgataat cccttttggt tgtctatgaa gcttaattct
     6841 tactaatgac ttcaatttag gaactttgat gaatttacta tcaatcaaag caactgtacc
     6901 attttgatta ttcgttgtat aactctgaac aggatttttc ttacttttga aacgtggaaa
     6961 tccaacggat ttatcccgaa aaaaattctt gtatgcttta tctaaattaa gttgggcatt
     7021 ggctaaagca aggctatcaa tttctttcaa aaacggaaat tctttcttgt atttagctgg
     7081 tgtcggaaaa gtcatttttt tagaagaatc atttttaact tcttcatacg ccttttttcg
     7141 gtcatcaagc attagattgt agaccttacg gacacaacca aaagatttgg caaagaagat
     7201 ttcttgttcc tctgttggat agattctgaa tttgtatgct tttagttgtt ccataaagtt
     7261 cacctacttt ctatttatga ttgtttcctt gattttggat atacttctta atcacatcaa
     7321 ttggtgcgcc accagttgtc agaagacaaa aacttttaga ccagaacatt tctttccaaa
     7381 gaaattgttt gactcttgga aagtcacgtt ttatcaatct tgagctggca cttttatagg
     7441 cattgatgaa ttttgtcaat tctgttttag gttgagcttt gaacataata tgaacgtggt
     7501 ctttatcatg attccactcc actaaagtaa tgtgatacga ttctgaaatt ctttcaaaag
     7561 tagttttggc ataatcagat atttcatcat caatcacttg tctacgatat ttcgttacta
     7621 aaacaaggtg gtaataaaga agaaatactg aatgattatt tgtatctaat tccatgttat
     7681 atcaactcat ttcttgtata gactgattgt aacatggaac aaaataaaaa ggcaatacgc
     7741 cttagttgtc ggtcttcgag ttaccaacgg taaccctcat accgaacggc attcatcacc
     7801 cacctataga ggatgggcga cttctgccta attctgttaa atattgtcaa gagggtttat
     7861 tttataaaac gtgttatttt aaaggttagt agacataatg tacactagac caagttgccc
     7921 ctctaccaat ggatagagac gctcactttg tgatttttaa ttggtctagg tcaa
//
