LOCUS       NZ_CP059769             5201 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterococcus faecium strain A11051 plasmid pA11051_P5, complete
            sequence.
ACCESSION   NZ_CP059769
VERSION     NZ_CP059769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675381
            Assembly: GCF_012933285.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Veerarghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Veerarghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 5201)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A11051
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.28x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 03:44:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,089
            CDSs (total)                      :: 2,997
            Genes (coding)                    :: 2,835
            CDSs (with protein)               :: 2,835
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 51 of 162
            Pseudo Genes (incomplete)         :: 102 of 162
            Pseudo Genes (internal stop)      :: 36 of 162
            Pseudo Genes (multiple problems)  :: 25 of 162
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059769.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A11051"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA11051_P5"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            215..1234
                     /locus_tag="HI861_RS15295"
                     /old_locus_tag="HI861_015295"
     CDS             215..1234
                     /locus_tag="HI861_RS15295"
                     /old_locus_tag="HI861_015295"
                     /inference="COORDINATES: protein motif:HMM:NF019371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_010729551.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQEM
                     IKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSFIR
                     NQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGHFNS
                     FFYSNQSPTFKHHHIKKNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCYDNPK
                     YKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQKLYLY
                     GGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKLKIMIV"
                     /name="Abi family protein"
     gene            complement(1681..2097)
                     /locus_tag="HI861_RS15300"
                     /old_locus_tag="HI861_015300"
     CDS             complement(1681..2097)
                     /locus_tag="HI861_RS15300"
                     /old_locus_tag="HI861_015300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098388136.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEK
                     QLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2099..2380)
                     /locus_tag="HI861_RS15305"
                     /old_locus_tag="HI861_015305"
     CDS             complement(2099..2380)
                     /locus_tag="HI861_RS15305"
                     /old_locus_tag="HI861_015305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125394241.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIDIQTKNTETLNDYVHTNNQIIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(2756..3280)
                     /locus_tag="HI861_RS15310"
                     /old_locus_tag="HI861_015310"
     CDS             complement(2756..3280)
                     /locus_tag="HI861_RS15310"
                     /old_locus_tag="HI861_015310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_125394239.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVVEKTKTENHKNANQVPQLEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTILSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(3298..4266)
                     /locus_tag="HI861_RS15315"
                     /old_locus_tag="HI861_015315"
     CDS             complement(3298..4266)
                     /locus_tag="HI861_RS15315"
                     /old_locus_tag="HI861_015315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002332718.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIAYFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     Typing          complement(3303..4266)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.661"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tatgatagta atgcttgata tataggcatt tcttttggtt gcacacttaa aaaaaatcac
       61 gattttgaca actaaatttt taaataaaaa agctttacag tttaattcaa atgtgtttta
      121 atatatctat cagtacttgc cacgcctctg atttaatatc atgcgcatta tggtagggct
      181 ttttttttat atttttttaa gaaagaagat tcttatgaag acatcaaata gaaaatataa
      241 agttaaaaca gttaatcata gaattccacc taaaaaggca ttgtgtgtaa ttaataattc
      301 caaagtaatt aatcaagagt taaaaccata tttatccgag caagaaatga taaaaaaaat
      361 aagtaaagat atttttttaa caaattccga tacaatttta ttagaaaagt ttttaaagaa
      421 aaatagttat tttcgattta gtatttatgt taaattaatg aaaaatatag ataaagttac
      481 tattctagat gttattgaaa cgtacaaatt agattcattc attcgtaatc aattgcatta
      541 ttttgtgaat caaatagaaa ttttttggaa aaaatctctt tcagataata tgtgcgtttc
      601 ttatgaagaa actagcatat ttccgaagaa tcagtgttat ttggataaaa atatttattc
      661 agatttgaaa tgggctgaag atataattgg tcattttaat agtttttttt attcaaatca
      721 aagtcccact tttaaacatc atcatataaa aaaaaatcat tgtttaccaa tatgggcttt
      781 atttgaagaa ataacatttg gtagtttaac aacatttatt aatcaactta atacagagta
      841 ttataataat tgggtcatga gctgttatga taatcctaaa tacaaaaaag ctatacgaag
      901 ttgggcaaac gtaattagag cttatagaaa taagatagct catcattcaa gaatatatgc
      961 atttaaagtt actgacgctc caacaatcat aaaaaatgat tccaaaagtt attttcctga
     1021 aaataactta aaagaaaccc aaaagttata tttatatgga ggattatacg ttataaaaca
     1081 tctgttaata tatgaagata gcatgacaca aactttatgg aataaatttc taatagattt
     1141 agaaaaaaag atcgataaaa tcccttcctt agaaaaatcc aattatggtt ttccggaaaa
     1201 ttggagaaac aagttaaaaa taatgattgt ttaattgtta tttaaattgt tagttaacat
     1261 aatgtacagt atgccaagta gcccctctac caatggatag agacgctcac tttgtgattt
     1321 ttcattggtc tagctaaaag agactttcta aagcttgatt tgatacgatt tgtttcttcc
     1381 atttctttgt gaatttggac cggtctgttt ttagggggtt gttatttttg tgagcgcttg
     1441 tgagactgct ctttcgagca gtcttttttt ttgattgttt gcgtataata aaaaagatcg
     1501 ttacattcgc aaaatttgtt acacttattt tacccttaaa acaagctaac tttttcttgt
     1561 ttagctgctc gtacgagcag cttttttttt tacgcaaatt gggatcgatt tgcaaatctt
     1621 attggaagac cgctctctag aaaataaacc aggaaattaa aaatttcctg gtttattttt
     1681 ttatcttttt agaaagttgg ttgggtcgaa atttttagaa gtgaatgttt gactcgtttc
     1741 tttttgacga ttctttggtt gatttcgttg ggagggattt agctgttttt caattttttc
     1801 ttttacttct tgatatactt tcgagtttgt accgataaga acgcctgtat cttttaaata
     1861 caaatcagca aattctgcaa tgatttcgga cataatagaa atttcttttc ccatcgcatt
     1921 tagttttgct tggttgctgg tagttgtcgt attagtatta ggttcataac tcatacatag
     1981 aaaacgaatg gcttccgagt agttattgat atctggattt ttttcgatta tggaatctaa
     2041 aacgtcaatg tgactgggat aaagggtaac gtgtttttga acagaagttt ttctcatgtt
     2101 atattcctcc gataagtgtt tcaatgattt ggttgtttgt atgaacatag tcgttcaatg
     2161 tttcagtatt ttttgtttga atatcgataa atttttcgag taataaatcg aattgatctt
     2221 ttttgtaatt cataagttga cggtcaaaat cactttcgat tagaactttt agatatttat
     2281 ttcgactcca tttttctcct gttttattcg tgagttcttg gcaacgtttg tctattgttg
     2341 caacaaactt tggatcaata ttccgaacta aaatgtccat aaaaaatctc cttttgcaat
     2401 tatgtataat ttatgtatat catacaaata ctatatttgt atagctgttt tttgccgaag
     2461 cactatacat aagctatcca taatggcgaa aaagtgatag caaatttttt tcttagaaac
     2521 gtggtaggca cagcctacct agcacctttc gtaagaaagt gataccacgt ttccttatgc
     2581 tctttcaaaa ttcagaaaaa tcagctgtaa aacgagtgca gtccgagtaa gattcaacga
     2641 aaaaacgggg attggggcgc caccaaaata tcgcaacatg ggcccatgtt gctctgctcg
     2701 caaaagcaaa aagaagaata aaaccagcaa tttaaattaa tttaaattgc tggttttatt
     2761 gccagaattg ccaccatttt ttctccgtct ttgattgttt ttctatcgat tctgtttctt
     2821 cagaaagaat cgttgattta ttttccgaag attcttccgt ataagtcaac tgtagttgtt
     2881 cctgcagttt ctcgatttgt tggttggctt gcagggtgag ttgttgttgt tggtcgagta
     2941 atttttgcat ttgttttatt tgctcgtctt tttcttgtag ttgcatatct ttttgattta
     3001 actgttgttc taggagagaa aggacttcta attggggaac ttggttggcg tttttgtggt
     3061 tttcggtttt cgttttttcg acaactagtt gttgatttga gttttcattt tcgttaaaaa
     3121 acattgattt tatagccttt tgtccggttt cattaatcac aaacttgttt ccgattttcg
     3181 aaaaccaaag ttttcgattt tcgtcagaaa cttttttgtt aattgctgtt ttactaactc
     3241 ctaattcttc agcaagttct ttgattgttt tttctgtcat aaaatctaaa aaccccctca
     3301 atctttgaat aagttttgaa gattgtctaa taagtctttt tttagttctg aaggaatatc
     3361 ggaatttatc tctgattttt gtgcattatt ttctttattt tgggtatttt gttctctttc
     3421 taacttttct aactgttgta aaagttccaa ttttcgcttt tgttctttga ttttttcttg
     3481 taatgaagta ggtgcttttt cagattgaac ttgttggtta gctagttcta atgctttgtt
     3541 ttgttcttct tttgttgggg catgtttgtt aaagtcgtct gattttttta tctcaggttt
     3601 aaatgtaaat tgatagccaa tgacaggctt tccccgtcct tttccgtatc tcttttggat
     3661 tgtcagacct ttgaagattg cggttagttc ttggcgaata ggctttataa tttttttatt
     3721 tatatcactt tctctataac ttttaggtat atcaagtaat tcatgaaagt cttcttttga
     3781 aaaataggca attcctttgg ttctgtattg ttttaataat ctaaacatgg ttttggaata
     3841 actgctttct aattcattga attgttggag agaatatcga acccattctt caagattatt
     3901 aagtaagggt aaggctttct catggacttg tatttcagca aaaggaatat cagcttttcc
     3961 atcgatttta aattgattaa ataaaacaaa tcgttcacgt tgcaaaccgt cagcacttct
     4021 tctaccaaaa cgtaaatcca ttaatttgtc atacgttgtt tctaaatcat ctatgaatcg
     4081 aacatttgca gttgctttat aattgcttaa atcttttaat tgttcgaatg taaattgtac
     4141 ttttttatcg cctacgtcac gcattctaga tactatagag aagaataaat tcatttctac
     4201 tgacgtaaat ttgcgtaaag gaatcgtatt taattcatgg tggtatttca caatttcatt
     4261 tgccataaaa tcctccttca agattaacta cttaacaaaa ttataaagag ctttctaata
     4321 ggggtcaagc ccctaattaa agacccctaa aaacagacaa aaagacccct aaaaacagac
     4381 aaaaagaccc ctaaaaacag acaaaaagac ccctaaaaac agacaaaaag acccccaaaa
     4441 gattctgcac accttgggag agtaagcata cagccgacgg taaagaggtt taaaaagagg
     4501 tttaaaaaga gattataaaa gaggagaggg gcaaaatttc ccgcctaaaa attttctgct
     4561 tttttagcca aacaggttcg cgaattttac taaactagga aacttgctcc ctctggtcgc
     4621 taaaaaggtg tgagggaaaa acaccctcac attgtacctg cgcagtcgcc taaaggctcc
     4681 tttgctaaaa acgttgttta aagagatttt tttagaggct ctgcgcgctc gtttaagccg
     4741 ttttaagaca gtttagtatg gataaaccct tatcttgttc ttaaagcacc ttacaggcgg
     4801 tcagggcaag ccctaacaac ccttaaagag cataaaacca tcctaaaatt cgatttaatc
     4861 tgtcttatct atgttcttgt atattttata taccttgggt agtaaaacgt ctgaaaacgc
     4921 aaataagagc gtatgaaaca aaacaataag caaccgattc agcagaattc cggaagagcc
     4981 attttcgtac gttagcaaaa aagtgctgtt gctaaaagct gcttgcggtg cggagcgaac
     5041 aaggtctcct agagcgagac gtgtacaaac aagcttacaa attcgtgaaa ggcaccggtt
     5101 ttgcgccatt ttcaagcaaa aaaaggtcta tcgagcttgc tcgatagacc gatcgcattt
     5161 agccccccgc attctaacag gggggggata atgtgaaaaa t
//
