LOCUS       NZ_CP059750            20166 bp    DNA     circular CON 03-OCT-2023
DEFINITION  Enterococcus faecium strain A7214 plasmid pA7214_P3, complete
            sequence.
ACCESSION   NZ_CP059750
VERSION     NZ_CP059750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675388
            Assembly: GCF_012933165.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20166)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20166)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 20166)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A7214
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.82x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/03/2023 03:40:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 48 of 121
            Pseudo Genes (incomplete)         :: 68 of 121
            Pseudo Genes (internal stop)      :: 33 of 121
            Pseudo Genes (multiple problems)  :: 24 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059750.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A7214"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA7214_P3"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            complement(15..311)
                     /locus_tag="HI844_RS14940"
                     /old_locus_tag="H4J16_014940"
     CDS             complement(15..311)
                     /locus_tag="HI844_RS14940"
                     /old_locus_tag="H4J16_014940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(446..1939)
                     /locus_tag="HI844_RS14945"
                     /old_locus_tag="H4J16_014945"
     CDS             complement(446..1939)
                     /locus_tag="HI844_RS14945"
                     /old_locus_tag="H4J16_014945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            2551..3504
                     /locus_tag="HI844_RS14950"
                     /old_locus_tag="H4J16_014950"
     CDS             2551..3504
                     /locus_tag="HI844_RS14950"
                     /old_locus_tag="H4J16_014950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            3476..3751
                     /gene="prgO"
                     /locus_tag="HI844_RS14955"
                     /old_locus_tag="H4J16_014955"
     CDS             3476..3751
                     /gene="prgO"
                     /locus_tag="HI844_RS14955"
                     /old_locus_tag="H4J16_014955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            3944..4198
                     /locus_tag="HI844_RS14960"
                     /old_locus_tag="H4J16_014960"
     CDS             3944..4198
                     /locus_tag="HI844_RS14960"
                     /old_locus_tag="H4J16_014960"
                     /inference="COORDINATES: protein motif:HMM:NF013677.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            4604..5766
                     /locus_tag="HI844_RS14970"
                     /old_locus_tag="H4J16_014970"
     CDS             join(4604..4880,4880..5766)
                     /locus_tag="HI844_RS14970"
                     /old_locus_tag="H4J16_014970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_127817621.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHL
                     YPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGI
                     QISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNKRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     gene            5942..6439
                     /locus_tag="HI844_RS14975"
                     /old_locus_tag="H4J16_014975"
     CDS             5942..6439
                     /locus_tag="HI844_RS14975"
                     /old_locus_tag="H4J16_014975"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_228780308.1"
                     /translation="MCEKWAADITYIPTKKNGWCYLSSIMDLHTKRIISYTFSKRMTVD
                     CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGI
                     ESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEK
                     MA"
                     /name="IS3 family transposase"
     gene            complement(6912..7484)
                     /locus_tag="HI844_RS14980"
                     /old_locus_tag="H4J16_014980"
     CDS             complement(6912..7484)
                     /locus_tag="HI844_RS14980"
                     /old_locus_tag="H4J16_014980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002303206.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(7500..8105)
                     /locus_tag="HI844_RS14985"
                     /old_locus_tag="H4J16_014985"
     CDS             complement(7500..8105)
                     /locus_tag="HI844_RS14985"
                     /old_locus_tag="H4J16_014985"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            complement(8303..8983)
                     /locus_tag="HI844_RS14990"
                     /old_locus_tag="H4J16_014990"
     CDS             complement(8303..8983)
                     /locus_tag="HI844_RS14990"
                     /old_locus_tag="H4J16_014990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            9122..9739
                     /locus_tag="HI844_RS14995"
                     /old_locus_tag="H4J16_014995"
     CDS             9122..9739
                     /locus_tag="HI844_RS14995"
                     /old_locus_tag="H4J16_014995"
                     /inference="COORDINATES: protein motif:HMM:NF013235.3"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_182482101.1"
                     /translation="MELLQTGINLHSIYMVDENENEIITAPAIARWVINKHTLEVTARI
                     NPEYLEWFSEVVNQYILFNLRDFVKLKSKYSMILFLELSTFWNTGMYYKRINDLREILG
                     VNPTRNNRDFINNVLKPAIAALKEYFPELSLTVEKNSSDSRKITHVRLKFDKLTITDMQ
                     ERLRSSEGASLVEYQQELENMEGEEKAVNDPTPEIDLSNVEW"
                     /name="replication initiation protein"
     gene            9853..10221
                     /locus_tag="HI844_RS15000"
                     /old_locus_tag="H4J16_015000"
     CDS             9853..10221
                     /locus_tag="HI844_RS15000"
                     /old_locus_tag="H4J16_015000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347150.1"
                     /translation="MENTNQTPNTAPTNQTSPAERKADLIRLYSRLEGMKLQHVPTAPK
                     APDGFPKSAESLEFFECKVCGSTFQTILRESVASGCPKCEHDRLGILADHLPEPDKNSC
                     LNNRKKPVTVMAMGFLCI"
                     /name="hypothetical protein"
     gene            10395..10703
                     /locus_tag="HI844_RS15005"
                     /old_locus_tag="H4J16_015005"
     CDS             10395..10703
                     /locus_tag="HI844_RS15005"
                     /old_locus_tag="H4J16_015005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303412.1"
                     /translation="MKEMKMKQVIYPVIIGVLAIALIILGVNCYNLSKENNQLETKNNK
                     QEIQIAKQAYVISDYKTYFRENYVDTVKYRSHFTDKQILNVYREALRDGKISDEGAN"
                     /name="hypothetical protein"
     gene            11008..11286
                     /locus_tag="HI844_RS15010"
                     /old_locus_tag="H4J16_015010"
     CDS             11008..11286
                     /locus_tag="HI844_RS15010"
                     /old_locus_tag="H4J16_015010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303413.1"
                     /translation="MEKKEDLLNAFKNLDEKDQLKALLRLHDITYSDIAERLTVKKGER
                     EGQKGVSLVTVQKAFQSKTNLESDRWKEIVKVATKLIEERQNDQNKA"
                     /name="hypothetical protein"
     gene            11417..11626
                     /locus_tag="HI844_RS15015"
                     /old_locus_tag="H4J16_015015"
     CDS             11417..11626
                     /locus_tag="HI844_RS15015"
                     /old_locus_tag="H4J16_015015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303414.1"
                     /translation="MIRKLNNIKLSLAILTLFFGIYNNSRMALFFLTLLIFMNLIIHNF
                     KELENTAPAPSQGNQESEGMPHAW"
                     /name="hypothetical protein"
     gene            11616..11888
                     /locus_tag="HI844_RS15020"
                     /old_locus_tag="H4J16_015020"
     CDS             11616..11888
                     /locus_tag="HI844_RS15020"
                     /old_locus_tag="H4J16_015020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303415.1"
                     /translation="MLGSFKKDKEVKTRGALTPSQQNKLGTKATFHPVRDQANQKELIG
                     VCVGFTSEKFFPIIECKIHGITTREVARKVVKWHTSQTKTNHKKR"
                     /name="hypothetical protein"
     gene            11936..12154
                     /locus_tag="HI844_RS15025"
                     /old_locus_tag="H4J16_015025"
     CDS             11936..12154
                     /locus_tag="HI844_RS15025"
                     /old_locus_tag="H4J16_015025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347151.1"
                     /translation="MNNKNTKNTNTNHEKRSFFDKLFKKKKNGKVSILKMIGWIFLAFF
                     IVAVNSSGGDKKEAASKDTTKGSVAKF"
                     /name="hypothetical protein"
     gene            12208..12894
                     /locus_tag="HI844_RS15030"
                     /old_locus_tag="H4J16_015030"
     CDS             12208..12894
                     /locus_tag="HI844_RS15030"
                     /old_locus_tag="H4J16_015030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_059355960.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLW
                     LFVNKD"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(12954..14249)
                     /locus_tag="HI844_RS15035"
                     /old_locus_tag="H4J16_015035"
     CDS             complement(12954..14249)
                     /locus_tag="HI844_RS15035"
                     /old_locus_tag="H4J16_015035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="WP_002297218.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRAC
                     RSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSY
                     FVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFL
                     VTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKL
                     KKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHEL
                     VEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
                     /name="ISL3-like element ISEfa11 family transposase"
     gene            <14494..14745
                     /locus_tag="HI844_RS15040"
                     /old_locus_tag="H4J16_015040"
                     /pseudo=""
     CDS             <14494..14745
                     /locus_tag="HI844_RS15040"
                     /old_locus_tag="H4J16_015040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /name="GNAT family N-acetyltransferase"
     gene            14838..15632
                     /gene="aph(3')-IIIa"
                     /locus_tag="HI844_RS15045"
                     /old_locus_tag="H4J16_015045"
     ARG             14839..15629
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            15992..16951
                     /locus_tag="HI844_RS15050"
                     /old_locus_tag="H4J16_015050"
     CDS             15992..16951
                     /locus_tag="HI844_RS15050"
                     /old_locus_tag="H4J16_015050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_002303350.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRFL
                     KSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIACS
                     VRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEFGHL
                     EGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFKSITF
                     DCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNGVSETF
                     IQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
                     /name="IS30 family transposase"
     gene            <17108..17191
                     /locus_tag="HI844_RS15645"
                     /old_locus_tag="H4J16_015055"
                     /pseudo=""
     CDS             <17108..17191
                     /locus_tag="HI844_RS15645"
                     /old_locus_tag="H4J16_015055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
                     /name="MLS leader peptide"
     gene            17240..17323
                     /locus_tag="HI844_RS15060"
                     /old_locus_tag="H4J16_015060"
     CDS             17240..17323
                     /locus_tag="HI844_RS15060"
                     /old_locus_tag="H4J16_015060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="WP_031929417.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuator leader peptide
                     ErmL"
     gene            17448..18185
                     /gene="erm(B)"
                     /locus_tag="HI844_RS15065"
                     /old_locus_tag="H4J16_015065"
     ARG             17449..18182
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="99.59"
                     /coverage="100.0"
     gene            complement(18448..19311)
                     /locus_tag="HI844_RS15070"
                     /old_locus_tag="H4J16_015070"
     CDS             complement(18448..19311)
                     /locus_tag="HI844_RS15070"
                     /old_locus_tag="H4J16_015070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="WP_001284311.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSG
                     KTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAIIS
                     RLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYETMY
                     ADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISP
                     KETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKT
                     PKLPGI"
                     /name="zeta toxin family protein"
     gene            complement(19313..19585)
                     /locus_tag="HI844_RS15075"
                     /old_locus_tag="H4J16_015075"
     CDS             complement(19313..19585)
                     /locus_tag="HI844_RS15075"
                     /old_locus_tag="H4J16_015075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="WP_000301765.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVN
                     LLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
                     /name="antitoxin"
     gene            complement(19602..19817)
                     /locus_tag="HI844_RS15080"
                     /old_locus_tag="H4J16_015080"
     CDS             complement(19602..19817)
                     /locus_tag="HI844_RS15080"
                     /old_locus_tag="H4J16_015080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="WP_001835296.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETA
                     KNGGNVKEVMDQALEEYIRKYLPDKL"
                     /name="peptide-binding protein"
     gene            complement(19909..>20166)
                     /locus_tag="HI844_RS15085"
                     /old_locus_tag="H4J16_015085"
                     /pseudo=""
     CDS             complement(19909..>20166)
                     /locus_tag="HI844_RS15085"
                     /old_locus_tag="H4J16_015085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /name="chromosome partitioning protein ParA"
     ARG             17449..18185
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
     Typing          complement(447..1939)
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaaataaaa actactactg gatatacttc ttcctttttc ttaactcgtt agctatttta
       61 tactcaatat actcctgctc taacacacac aaacaatcat atacctgatc catagtctcc
      121 ccagaagcta aggacaaccc atgaagaaac tcaatcggca aagaagcaac tgttttttcc
      181 ctggtaaccc aagaagacaa agtcccctgc ttaaaaccat aaagctcaca gaacacttcc
      241 acagataatc ccaaacgacc aatgataaac acattaacag gatgtgaata gacataattt
      301 tttaacgaca tacaacttcc tccatgatac ataagactgc atacgcagtt ataagtatac
      361 cacctacacg tagctaggca aagggagcaa cacctacaga agtatcatag atatattgta
      421 taaggcaatt tgcacctttt atttttcaac ctgtatctac ttcaaaaaga ttcacttctt
      481 taagagggtt caagggggtt gataattgtc taaatgtctc ttctactaag ctctcgttga
      541 gtccaaatac ctctgtgatc cttgttgaat aatctagtct cttttcttta cttagttgaa
      601 taaccctcgc gaacaacgtt ttgactgaag caatgatcat tccgccattt cttcctcgtg
      661 ttaccttgta gaagacttta ttctcctctc gtaactcatt taagatctta tcaagtgttc
      721 gcttgggcat cgttagttgt gaagccaatt ctttttttga caggttcaaa taaggctcac
      781 tagcaagact tttttcgctt agataagcca atagatcttc tttccattca tttttatgac
      841 tatattttct ttgatctcgt ggctttttaa atttccacca gccacgtcgt tgaatgaaca
      901 gatcattatt ttttaaggtt ggtgaaaccc attgctggca caattcaata atgaattctc
      961 tgcttgctgc ttgataatta ccagaataag cactacgtac aatctttact aattctgctt
     1021 cagatagcgg ttcctgtaac cgttcgttga atgtgaacat attgtattcg caagtatcca
     1081 atgcataacc tgaagagtaa taagctaaag aaagcgtgaa aatcacatta tttcgaccaa
     1141 gacgtccttc tccgccgacg atgtttgctt tatgaagtaa catatcgaac cacggttcat
     1201 ctacttgacg aatttctttt ttctcaggaa agacaattag ttttcgttct cgatcttctt
     1261 ttgttttttg atctgattca agcttcatcg accattgaat ccagtcagca aaagaatact
     1321 gatagttcat ttctttaaaa acaatattat tttcttgtgg gaatcgagca attccgaagt
     1381 ggttacatcc tagatccact ccggacaatt tttcagccaa ctgtagacga attgtttttg
     1441 aaattttttt tgctacatcg atcaccttga aattgctttt ctttgtgaca tagcctggtg
     1501 tctttagtac aaaataaact tgataaccat gatctgattc taaaattagc gttggcatgt
     1561 agcccacttg atctaaacag gctaaaataa tttcgccttg atggttctct tttgaatcga
     1621 tatcaactac aaatgtgttg atttgactta agtttttctc agaatgacct ttaaccactg
     1681 ttcgataatc atccgcataa gtaccaaagc gataggtatt aggtgtccaa tgcgttagtt
     1741 tcttttcgtt ctctaaaata gcttcttccg aagtaatgac cataccacgc ccaacattca
     1801 ttaattgctt gctacggtac gcaaagatag caccactcct actggaatca atatgacctg
     1861 caggtttcat ggtgctgttt cgatatttgt atgtacgtaa gccgttttta agaatagctg
     1921 aataaatttc gctcaacatc gattgtaacg tcctttctca aacaaaaaga gaacacagtt
     1981 tagctttaat ctcagccata gtatgtgttc tctcttcagt gtttaagaat atttaattta
     2041 agtacactaa ataaacgttc ttctgttgag ttgctcgcct ttttttggta gaataaaggc
     2101 aatcggaaaa ggtatccgat caacttttaa tataagtagt tggtagctac ttacatattc
     2161 ttgagacgag cgtgaacaca tgtcctaacc atgtgattca gaaataattg attctgctaa
     2221 aatcaattat ttccgtctct tttttttatg taatttttct gtttaaagag taccactgct
     2281 tttgctactt tgtaaatagt gaattatctg tggtttctat agaattaagt ttacctcttt
     2341 tcactctaaa ttgtcaacgc aaaacgcaca aatatatttt gacattttga atatatctga
     2401 tatactgaat atagcaaata tatttagtat atccactata ttccttataa agtctgaatt
     2461 cttatcaatt tggagtatat tagatatatc gaatatatta agtatatgaa gtatactaaa
     2521 tatatttagt ataggaaatt ggagggaact atggaaaact tacggactgc atttaaaaca
     2581 tcagataaac taggaaaagt tgtaatgctt acaggtagaa aaggcggcat tggtaagact
     2641 acagacaatg atttattagc gattgtttct agtcaacttt ttgaaaaaga cgtgttgcta
     2701 attgattatg accaacaaag aaatactact tccaacattg gatcaacgta tcaaattact
     2761 agctttgatc gatccatgtc tgcagcaatc aaaaaagggg actgggtttc aggtattact
     2821 caagtaagtc cacatttata tattatggct ggctctcctg gatctgaaga acttaatgaa
     2881 tacttgtccg aaaaatatcc tgatcgtcgt aagagatcat tggccttcat taaaccttta
     2941 gaagaattga gaaaaaattt cgattatatc tttatcgact gcccaccatc aacagataat
     3001 gtggttcgtg cattcttaac tgctgcagat tatattattg caatgcaaga attaaaacgg
     3061 tacgcaatgg aaggaacaga agattttatt aataaagttt tagttccgat tgtcacgaat
     3121 tttgaagagt ctcatcttca aatcattgga attttgcctg ttcttttctc agtgcgacgt
     3181 tcctctcaac acgcaaacta tcaaaaaaca attgataagt atggtgaatc aaacattttt
     3241 aaaagcattg ttaaaggctc ggatcggtta gaaatgtatg gtgagaatgg tatccaatta
     3301 aatgattatg tagatcgaag atggtgggct atttttgcag acatctttac agaattagaa
     3361 gaaagaattg aatactatga acaacatggt gatattgaag gatttgatta tacacctaaa
     3421 tatgctgact caatggccaa tcatatccta cctcttggaa aggagattaa actaaatggc
     3481 attattacca acgaacaaag ataagaagaa aattggtgct gaagatattt caattaaaga
     3541 tgatattaat gtcgagttag gggaacgaaa gtacactgcg aaagaaagaa aaccagttca
     3601 agtagatcct cccgtactaa aaaccattcg ggatcttagt tatgcaaaag atattcctat
     3661 gtatgaaata gtcaaacttg caatggacgc ctatgttgat actttaaacg acgaggaacg
     3721 gattatatat aatagacgat caaaagatta aaatatagta agtatactag atatagtaaa
     3781 tatatttagt atacttacta tatttttagc tttattaatc ctaatccttc aaaatatgtg
     3841 tattgtttgg gggtatacaa catttggtgt tagtatcaaa tcttagacag cttttcgtag
     3901 agacaagaat aaattaaact tatctacgag aggatgaatt tttatgaccc gatatgaaga
     3961 aaattttaaa caaatgattg ttgaactgaa tcaaactgga cgttctgttc gagggttagc
     4021 gaaagaatat ggcttatctg aagcaacgat ttacaaatgg aagaatttat atttacctga
     4081 tcagtccaca ggactgactg gaaaagaagt agctgaactg agaaaagaaa atgctcgttt
     4141 aaatgaggaa cttgaaatct taaaaaaagc cgcagccata ttctctcgga aaacctaaat
     4201 tcgcttgttc aatttattga aaaatggtgt aaggattaca ctgtttcttt gttgtgtcgg
     4261 ttattagaaa tccctagaag tgtctattat ttttataaga ataaaccgtt gacagctaca
     4321 gaaatcagaa ataataagtt gaaaaagaag atttcaacaa ttttttttac gaataaacaa
     4381 cgctatggtg ccacaaagat ccatcaagtt ttattaaaag aagggatttc agtatctctt
     4441 aaacatgtcc aaaagctaat gaaacaatta aatttaaggt cgattgtagt taaaaaatat
     4501 agacctcaaa ggtctaataa accgatcatg gataggctac aattaactag acagaaaaat
     4561 taaggtgtgt agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg
     4621 aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc
     4681 acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa
     4741 gcaatctaaa acgagtggtt cattcaaaga aaaagataat cttacgcctg aacaaaaaga
     4801 attgttagaa ctacgtaaaa gaaaccagca attagaaatg gaaaatgata ttttaaagca
     4861 agcagcgctg atattcggac gaagagacaa gtaatcgatg cgaataagca tctttaccct
     4921 atatcagcga tgtgcagaat attaggtcta tcacgtcagt cctattatta tcaatcaaaa
     4981 ccaaagaaag acgaatcaga acttgaagaa gtagtcgctg aagaatttat ccgcagccga
     5041 aaggcctacg gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat tcagatcagc
     5101 cgacgaaaaa ttagtagaat catgaaaaat agaggattaa aatcgagcta tactgttgct
     5161 tattttaaag tacatcattc tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt
     5221 aaattcttaa gagacaaccc attagaagcg atcgtaacag acttgactta tgtacgagtc
     5281 gggaaaaaat ggaattatgt ctgtttcatt ttggatctgt tcaatcgaga aattctcggc
     5341 tattcttgtg gagaacataa agatgccgtt ctagtaaaaa aagcatttag ccgtatcaaa
     5401 caacctctga cagaggttga gatttttcat actgatcgtg gaaaagagtt tgataaccaa
     5461 gctattgatg aattattaac aacttttgac atcaatcgat cattgagtca taaaggctgt
     5521 ccttttgata atgccgtagc tgaatcaact tataagtcgt tgaaagtaga atttgtctat
     5581 caatacacat ttgaaacctt acaacaattg gatttggagt tatttgacta tgtcaattgg
     5641 tggaaccacc ttcggttgca cggtacactt ggctacgaga caccggttgg ttaccgtaac
     5701 aagagattgg cgcagcgaat ccttgataat gagctcggat gtgctaacgc tagcgaggca
     5761 gtctaacttt aactgttagc tcctgccgaa gatcgtcaca tccgaggagg ctcattgtca
     5821 aggacaatcg gaatagcata cggaaagaag ttctgcacct tataaaattt gtcaaaaaaa
     5881 ctgttgccat tccatcattt caaaagagaa tctcttaaat caggactttt ctactgaaac
     5941 gatctgtgag aaatgggcag ctgacattac gtacattcct actaagaaaa atggttggtg
     6001 ttacttatct tcaatcatgg atttacacac gaaaagaatt attagttata cattttcaaa
     6061 acgaatgact gtggattgtg tcattcaaac attgaataaa gcaaaaatac attatcacat
     6121 tccagaagga atgattctac acactgactt gggcagccaa tacacagcaa gagaagtgga
     6181 acaatggctt aaaaccaaca aaataaggca ttcttatagt agaaaaggaa caccttatga
     6241 taatgctgga atcgaatctt tccatgcctc attgaaaaaa gaagaagtct acacgactag
     6301 ctactcagat tttgaagaag caaatcgagc gctatttagc tacattgaag gattttataa
     6361 ccgaaatcga atccatagct cgattcacta tctaacccca caggaatttg aagaattagc
     6421 aaaagaaaaa atggcttaac cgtctctact aaaatgtgtc caagatattg actcaaatcc
     6481 agtaatatca caataggaat gctgtttatt atattagcag cagtgctgat ttttatgata
     6541 gttatttggt tcttaaatga aagatctaaa gacaatcaac accgtaggga tatggagata
     6601 aaatcaatag agtcaataga ttatttatcg ataataacac taaaaaagcc acgttcctaa
     6661 acgtgactta cgctgaacga tatgtgttgt ttggtagacg aacaaatcgt tcattataaa
     6721 tatatgatgg tatcctaata tagtaagcgt ggctagcata cctttaatta ctttaccttt
     6781 tacagttacc atacacattt tttgagcaga aatcaagtaa ataaaaaaat attatatttc
     6841 acaacatgct aatagactca tatctagaac gcattaagta tttacctgtc tggttcgctg
     6901 agacttcttt tttaactttc ttcaattttc ttctttattc taaaaagcgt actgcgactg
     6961 aaaccggtta tagcttcaac ttccttatac gatttacctg aagtaagcag ttcatatgcg
     7021 tgcctcttct tcggtgtgat tgttgctttc ggacgtcctt ctctgaaatt aggattattc
     7081 tttcttgcaa acatttttcc ttcttgggta cgttccacaa tcatatcacg ttccatttct
     7141 gctacactca acaacgtccg tgtcaccatt cgccccatgg acgtattttc aatcgttcct
     7201 agattcaaaa ccttgatcgt tacgttatca tcgagtaaag gttcaatgat ctctaatgct
     7261 tctctcgtgt tacgagcaaa tcgatcaagc ttcgtaacaa ctatttcatc tccaggcttc
     7321 agaccagcca acagctgatt gaattttggc cgatcccgtt ttgtacccgt aaatttttct
     7381 gaaaaaatat tcttcttctc tacgcagttc tcaagcaact gttgaatttg ttcttttgta
     7441 ctttgagaag cggttgagac acgtgcatat ccatatttcg tcatttggtc cacctacttt
     7501 tacaaatcat ctatcgtata ctcagaatac ccttcataga aatagctgac atcgattcct
     7561 ttcatatata actcgcgatc gtcaatttta tcagtcagag cattggaaat tagatagcgt
     7621 atttccaaat cgtttatggg acttcgctcc attgcagaaa gatagtctgc tttatcgatt
     7681 aagttccaat ctacaacttt ttgcaattct tcctttaaaa tgagatccag ccaaattcta
     7741 gtacttcggc catttccttc tcgaaatggg tgagcgatat tcatctccac atatttcttg
     7801 ataatctctt caaaagaagt ttgtggcatc tgatcaatat gctgtaacga atgttctaaa
     7861 tacataactg gtgcaaatcg aaaatttcct tttgcaatat tgaccgatcg gatttttccg
     7921 gcaaaatcgt aaatatccga aaatagaaat tcatgaattt cagataaacc tttaaatgta
     7981 ccaacttcga tttcatttat ttttccacaa tcgtacaatt ctttcgcttt ttgtttactg
     8041 agtttttctt caaccttagc caactcaact tgattggtta atcccaattt attctctaaa
     8101 atcatgcgaa atctccttaa ctttatgaca taagtttatg aaacactctc aaccccatta
     8161 tatcgcgatt ttgtttatgt gtcaaaaact attaagtttc tgacccccga tagatttttc
     8221 aagtgtctct attttgcaca gtctagttgg ttctgttgca aagttttaaa taaagaataa
     8281 aatcccttac ggtatctatg atttaagctg ggattcccaa taataccttg atttcagtac
     8341 agaccgaaaa cccgaagaga gtgccttctt ttcgggtttt cttatataat cctcgaatgg
     8401 cttccatgcc tttaatcgtg gtagaggcag tgcgtaaact tcgatagaat ttattgcgtc
     8461 tctttactgg acgatggtct tgttcaatca aattattcag gtatttaatg gtacgatgtt
     8521 ctgtcccttg ataaaagccg tattctttta gtttcttaaa ggcacttgta atagaggggg
     8581 ctttatctgt gactacaacc ttcggttcat caaactgctt cactaaccgc ttaagaaaag
     8641 cataggctgc ttgtgtgtcc cgttttttac gtaaccaaat atccaaggtt aaaccatctg
     8701 catcgatggc tcgatacaaa taatgccatt ttcctttaat tttgatgtac gtttcatcca
     8761 ttttccatga ataaaaggat tttttatttt tctttttcca aatttgatag agtagtttgc
     8821 catattcttg cacccaacga taaatcgtcg tatgagaaac gttaatgcca cgatcatata
     8881 agatttcttg aacttcacga tagctaaggt tataacgaag atagtagccc acggctacaa
     8941 taatcacatc ctgctgaaat tgctttcctt taaaatgatt catcgtcatt actcctgcta
     9001 tctttttcta ttattctacc ttatttgata gtagatttaa aactttgcaa cagaacccaa
     9061 caaacaaata gttgatcgtt acttttcaaa ccagccagaa cctctgacaa ttaacgagca
     9121 aatggaatta ctccaaacgg ggataaattt acactctatt tatatggtag atgaaaatga
     9181 aaatgagatc ataaccgcgc ccgctatagc aaggtgggta ataaataagc atacgctaga
     9241 agtaacagcg cggatcaatc cagaatattt agagtggttt tcagaagtgg ttaaccaata
     9301 cattttattt aatctaagag actttgtgaa gttaaaaagt aagtactcca tgatcctttt
     9361 cttagagctt tctacctttt ggaatacagg aatgtattat aaaaggatta atgatttaag
     9421 agaaatttta ggagtcaatc caacgagaaa taacagagat tttataaaca acgttctaaa
     9481 gcctgctatc gctgctctca aagagtactt tccagagcta agcctaacag tggaaaagaa
     9541 ttctagcgac tccagaaaga tcactcatgt aagattgaag tttgataagc taacaataac
     9601 cgatatgcaa gaacgcctaa gaagcagcga gggagcttct ttagtagagt accaacaaga
     9661 gttggaaaat atggaaggag aggaaaaagc tgtcaacgat ccaactccag agatcgacct
     9721 ttccaacgtg gaatggtaac acctgtcagc aatctaatcc aataaaccaa agtacttgta
     9781 aggcgcgagg gctcctttta gagccttcta gtcctatacc aaaccaagca aaataaagga
     9841 gctataacca acatggaaaa cactaaccaa acaccaaata cagcaccaac taaccaaact
     9901 agcccagccg aacgcaaagc cgatcttatt agattgtact cacgccttga aggtatgaaa
     9961 ctccaacacg ttccgaccgc tccaaaggcg ccagacggct tcccaaaatc ggcagagagc
    10021 cttgagtttt tcgagtgtaa ggtgtgcgga tcgacttttc aaacgatttt aagggaatcc
    10081 gttgcgagtg gctgccccaa atgtgaacat gatcgcttag gaattttagc cgatcattta
    10141 ccagaaccag acaaaaacag ttgtttaaat aatcgaaaaa aacctgtaac cgttatggct
    10201 atggggtttt tgtgtatttg agataatttc caagggttat ttatggattt attttaaaaa
    10261 atctattaaa ttatactacc actggtagta atataaatat atcgaaaggg tacgagggtt
    10321 aaagcgtaat ggcggaatga gtaccgttct cttttgaaaa taccaaacca accaattaaa
    10381 ggagagaaag taatatgaaa gaaatgaaaa tgaagcaagt aatttatccc gtgattattg
    10441 gagttttagc tatcgctctt attatccttg gtgtaaattg ctataactta agcaaggaaa
    10501 ataatcaact tgagaccaaa aacaacaagc aagaaatcca aatagccaag caagcttacg
    10561 ttatcagcga ttacaagact tattttcgtg aaaactatgt agacaccgtg aaatatcgct
    10621 cccacttcac tgataaacaa attttaaatg tttatcgaga agctcttaga gatggaaaaa
    10681 tcagcgatga gggagctaac tagtggaaga gctcattttg ctggctatgt tattacagga
    10741 aaagaagcgt gaacgagaag cctaacagct ggaaagaatc cgaataggat atgaggaagc
    10801 agatcgggaa agagatcaga aaatagagga gctggagaag cgtatccaag aactttccaa
    10861 aaactatgat aataaccaag attgggaaca agagtggcgg aaaatcgttg gtataaagga
    10921 aaatgaatac ttagtgagaa agccgccaga agaagaggaa gacccttgga gcgatttcta
    10981 actaaaaatc ggaagggagg tggtaagttg gagaaaaagg aagatttatt gaacgctttt
    11041 aagaacctag acgagaaaga tcaacttaaa gcgttattaa ggcttcatga tataacatac
    11101 agcgatattg ctgaaagatt aaccgttaaa aaaggcgaga gggaaggaca aaaaggtgtt
    11161 agcctcgtga ctgtacaaaa agctttccaa tcaaagacca acttggagag tgatcgctgg
    11221 aaagagattg taaaagtcgc taccaagcta attgaagagc gacaaaacga ccagaacaaa
    11281 gcataaaaaa agcccgtcca tttaccgctg accaaagcaa taaaatgaac gagccacacc
    11341 atttactttt aggggtaacc acacgcccta aaagctacca aaccatttac caaataacca
    11401 ctaaggagat ttagaaatga ttagaaaact taataatatc aagctttctt tggcaatttt
    11461 aacactattt tttggaattt acaataattc cagaatggct ttgttttttc ttacgttatt
    11521 gatattcatg aacttaatta tccataattt caaggagtta gaaaatacag ctccagcgcc
    11581 ctctcaagga aaccaagaaa gtgagggcat gccacatgct tggtagtttt aaaaaggata
    11641 aggaagtaaa aacacgagga gcccttacac caagccagca aaataagctt ggtacaaaag
    11701 ccactttcca ccccgtccga gatcaagcca accaaaaaga actaattggc gtttgtgtag
    11761 ggtttacttc agagaaattt tttccaataa tagagtgcaa aatacacggg atcacaacta
    11821 gagaagttgc tagaaaagtt gtgaaatggc acaccagcca aaccaaaacc aaccataaga
    11881 aacgttaaaa acgttcctaa gagcgtttga aaattaaatt aagggagatt taagaatgaa
    11941 caacaaaaac accaaaaata ccaatactaa ccacgagaag agaagcttct ttgataagct
    12001 tttcaaaaag aagaagaatg gaaaagtatc tattttgaaa atgatcggct ggatattttt
    12061 agcctttttc atagttgctg tgaacagtag cggcggtgat aagaaagagg ctgcttcaaa
    12121 agataccaca aagggttctg ttgcaaagtt ttaaatctac tatcaaataa ggtagaataa
    12181 tagaaaaaga tagcaggagt aatgacgatg aatcatttta aaggaaagca atttcagcag
    12241 gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt
    12301 caagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg
    12361 caagaatatg gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat
    12421 tcatggaaaa tggatgaaac gtacatcaaa attaaaggaa aatggcatta tttgtatcga
    12481 gccatcgatg cagatggttt aaccttggat atttggttac gtaaaaaacg ggacacacaa
    12541 gcagcctatg cttttcttaa gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc
    12601 acagataaag ccccctctat tacaagtgcc tttaagaaac taaaagaata cggcttttat
    12661 caagggacag aacatcgtac cattaaatac ctgaataatt tgattgaaca agaccatcgt
    12721 ccagtaaaga gacgcaataa attctatcga agtttacgca ctgcctctac cacgattaaa
    12781 ggcatggaag ccattcgagg attatataag aaaacccgaa aagaaggcac tctcttcggg
    12841 ttttcggtct gtactgaaat caaggtatta tggctctttg tcaataagga ctgatgagtt
    12901 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcagatttt ttgttatctt
    12961 acattgatta attgattgat caaaaagatt ctaattctca tgttctcaaa tgatttaaat
    13021 ccgtaggata ctcgtttcat tgtctttatg tgggtattct tcgcttctat ttttccattg
    13081 gaataaggat agatcattgc gttggtgatg ccttcttcat aggtcagaag gttttgaagc
    13141 ttttcccgaa agctgtcatc taacgtttcg ggaagttctg ccaataagga gaaaaataag
    13201 tcagggtctt tgtctcgaaa ggcttcaact aattcatgaa aaaagggata cgcctccttt
    13261 aggggcggag aaaactcaag caatcgatca atcatcattg cttcagtaag aaatgggtat
    13321 tttggtgctc ggaaactttt ccatgttttg tattcataat ggttgatgtt tgcacgattt
    13381 tttagcaaaa agcgccagtt ctttttcagt ttttctgcct ggcttttctg tccggcttta
    13441 cgaagttcat tcatttcacg gatacgcaac tcattgaacg cttgattcat gtgtttgaca
    13501 atatgaaacc gatcaatcac gactttcgca tttggcagaa cacgtttggt gagctggaag
    13561 taggcggcgt tcatgtctgt caccaagaat tctacttctt ctggattggt acaacctaag
    13621 aaatagcttg ttaatcgagg taatttacgc gtaggcaaaa catctattaa ttttcctgtt
    13681 tcgccatccg cgcaaataaa gctcatctta tcttctatgg aagcatgcga acgaaattcg
    13741 tcaaccatca atactctggg gaggatcttc ttagattgct ttggtaaata gcttttaaac
    13801 tctttcaatg tacgaataac ggtggtcaaa gatacctgac agcttttcgc aataaaagat
    13861 aaagatactt tttcagtcag taaagaagca attttatatc taacatgatt tgcgattgaa
    13921 tgtctgggtt ggacaaaata actttgagcc gtccaatggg tgcgacagtt tttacaggta
    13981 tagcgttgct tttttaggcg cataaccaaa ggcatatgat tgtattgttc aaaacggaca
    14041 atcgtttcct tttttccatt tttcactata attgctttcc cgtttccatc taccacagta
    14101 gaaccacaac ttctacaagc acgaggagta ggcgagagaa cagcatcgac gaccaacgtc
    14161 tttttcttct gaagggtctc gtaagagacc tctgtaatca tcaaatcttt ctctgttatt
    14221 ctcagcattt ttttgataga atcattcata tagcgtatcg tcctctcagt tgtttatttt
    14281 gtggtgattt aatcatacta gagaacgata tgttttttaa tacctaaaat gaaaatgggg
    14341 ctgaagaatc aattctgatt catcagtccc ataaattata gagcccattt cttgggagtt
    14401 ttgagactac tatatgcgtt tttttgttga tttaccaaca tgtttagaca ttttcctcta
    14461 ggaaaaaatt aactagcacc acgggttatc ttttatatag aagatatcgc cgtatgtaag
    14521 gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag
    14581 cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa
    14641 ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
    14701 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
    14761 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
    14821 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
    14881 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
    14941 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
    15001 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
    15061 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
    15121 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
    15181 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
    15241 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
    15301 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
    15361 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
    15421 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
    15481 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
    15541 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
    15601 aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc taaaaagctt
    15661 taactacaag ctttttagac atctaatctt ttctgaagta catccgcaac tgtccatact
    15721 ctgatgtttt atatcttttc taaaagttcg ctagataggg gtcccgagcg cctacgagga
    15781 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
    15841 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattacgc
    15901 cgattgtaaa attaagctag acaactaaaa aaagtcattt gtgctacact caaaatagtt
    15961 ccgacgaaag aattataagg agtgagtaca aatgacctat aaccatctta caccaactga
    16021 actcgtaatg atagaagctt atttcaatca atcacaacct gtttcaaaag tagccaatct
    16081 gttacaacgt tctcgacaaa ctatttataa ggtctatcgt tttcttaaat ctggtggaag
    16141 cgcaatgagc tactataaac aatataagaa aaacaaacgt aactgcggaa gacatccgat
    16201 tgttttacct gatgagcaac aggaatacat tcaaaaaaag gttgttcaag ggtgggctcc
    16261 tgatgtcctt attggtcgag cagagtttcc tattgcttgt tcggtacgca cactgtatcg
    16321 aatgtttaaa gataaaacct ttgacactca cgatttacca atgaagggaa aaagaaaacc
    16381 aaacggtcat aaagaaaaaa gaggaaaaca agggtttcgc cggtcaatcc gagacagaaa
    16441 gacagactat acccagtttg atcatgaatt tggtcacctg gaaggtgaca ctatcgtggg
    16501 tggaaaacat aaaagcgcgg tcatcacatt agttgaacgg ctatctaaag tcatcattac
    16561 cttgaaaccc gaaggcagac aggctaaaga cattgaaaat aggctgaatc aatggtttcg
    16621 ttgtgtccca aaaaacttat ttaaatccat cacatttgat tgtggaaaag aattctcaaa
    16681 ttggaaaaag attagtaata ccaacgatat ttcaatttat tttgcggatc caggaacacc
    16741 ttctcaacga ggattgaatg aacattcaaa tggcttatta cgtaaagatg gtttgtataa
    16801 agctatggat tttaatggtg tatctgaaac ctttatccaa tcggtggcat cgaaacgaaa
    16861 tcatattcct agaaaatcat tacactatcg gacaccaatg gaagtatttt tgagttacgt
    16921 cacaaaagat gatttgtcta acttaatttg acaaacgaaa ttataaaaaa gtggtgcatt
    16981 tttaaattgg cacaaacagg taacggttat tgcaggtgta tttcttatct atgggtttaa
    17041 catggatttt atcattaaaa tcatgagtat tgtccgagag tgattggtct tgcgtatggt
    17101 taaccctaaa gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca
    17161 ttatcttaaa attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat
    17221 taagaaggag ggattcgtca tgttggtatt ccaaatgcgt tatgtagata aaacatctac
    17281 tgttttgaaa cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc
    17341 ctaccagtga ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt
    17401 taacttctgt atttgtttat agatgtaatc acttcaggag tgataacatg aacaaaaata
    17461 taaaatattc tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat
    17521 tgaatttaaa agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga
    17581 cgaaactggc taaaataagt aaacaggtaa cgtctattga attagacagt catctattca
    17641 acttatcgtc agaaaaatta aaactgaata ctcgtgtcac tttaattcac caagatattc
    17701 tacagtttca attccctaac aaacagaggt ataaaattgt tgggaatatt ccttaccatt
    17761 taagcacaca aattattaaa aaagtggttt ttgaaagccg tgcgtctgac atctatctga
    17821 ttgttgaaga aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct
    17881 tgcacactca agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta
    17941 aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata
    18001 aatattggaa gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac
    18061 tgtttactaa aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta
    18121 ccgttactta tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga
    18181 aataattcta tgagtcgctt ttgtaaattt ggaaagttac acgttactaa agggaatgta
    18241 gataaattat taggtatagg gcacctctaa taactaccaa ttaattcacc tcaaaatgaa
    18301 attagaaaaa aacagcgaaa tttttaaatc tatttcttat cgatacaaat tcctcgtagg
    18361 cgctcgggac cccaacatga ggaaatgttt ttataaaaaa agagtagtta ccgttttttg
    18421 gtaactactc ttttcctttt ttaaggttta aatacctgga agtttaggtg ttttgggtat
    18481 tggtggtgta ggtggctgca agctttccaa tttttgttga attgctttaa attcaggtgt
    18541 ctcttggtgt ttatttagaa ccattttttg ctctattcgt tctaaagtcg gttgaatttc
    18601 tttccctgat actttacgat tcaattcttt ttctaaggtt tcttttggac taatagaagg
    18661 tgtttccaaa cttgaataga gttttactcc ttctctgtta tatagcctta tatcgctaaa
    18721 taagcccgtt ttatgaaggg tttctaaatt ggtcggtaag tttttgacaa caatatcatg
    18781 cgcttgtttt ggtgttgccc tggctgtcat tggatcatct gcatacatgg tttcatatcg
    18841 ttcaattgtt cctaaatatg agttgatttt aggtactgcc atgacataca tttttgtttc
    18901 ataacctttg gcttgaagca ttgttgcggt ttgaatagga acgtctgttg ttcgtcctgt
    18961 accttcgatc accaaattat acccttgatc gctcaaacgg cttatgatcg cttctgtcat
    19021 gcgattagaa taaggggtaa cgtgttttac tacgtctttt tcataaagtt tcactagttc
    19081 atcaaaatta gggtgctgtt gtttaaaggt atcattatca atgacaataa cattcccttg
    19141 tgtttcttca aaaattgctg atcgcaaact ggttttccct gaccctggtt gcccaccaag
    19201 taaaaaagcg gttggcgatt caaccgcttt ttttccttga atcaattctt ctaaattatc
    19261 atttaagcga ttctcaaatt gtttgtcagt aaaattgact atatttgcca tattaagcca
    19321 ctttctcttt attcaaaact tgttttgagt aacgattcat tgaccgccaa tactcatgaa
    19381 cggcttgtaa ttcttctaaa gaataatcaa aaagatttac acgttttgca agctttaatt
    19441 ggtttaataa attgacttcc aataattgag agtcattttt atttaattca tggttcaaaa
    19501 catagtttaa tactcgatta taaacgcttg ccgataattc gttaatgatc tctatttcaa
    19561 atgttttttc atacgtaact gccatttcat tttcactcct tttaaagttt gtcaggtaaa
    19621 tatttccgta tatattcttc taaggcttga tccataactt cttttacgtt tccgccattc
    19681 tttgctgttt cgatttttat gatatggtgc aagtcagcac gaacacgaac cgtcttatct
    19741 cccattatat ctttttttgc actgattggt gtatcatttc gttttgcttt ttgtgcgcct
    19801 aaatttccca caatcactca cttctttcta tttcttctta ctcttatttt atcatcaaca
    19861 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgattct attctttttc
    19921 gttttctaat tgaataattc gctcaagcat ttcaaaaaat acgttcttat acatggataa
    19981 aacttttttg tcatagcctt tatgctctgt aatgccgttt ttagaccagg tacttacttt
    20041 attacttcgc ttgataatat tttggaaaac caagttatct tctttatgtt gcttatacag
    20101 ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag gaacaaaacc
    20161 gatcat
//
