LOCUS       NZ_CP059749            27932 bp    DNA     circular CON 03-OCT-2023
DEFINITION  Enterococcus faecium strain A7214 plasmid pA7214_P2, complete
            sequence.
ACCESSION   NZ_CP059749
VERSION     NZ_CP059749.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675388
            Assembly: GCF_012933165.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27932)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27932)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 27932)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A7214
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.82x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/03/2023 03:40:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 48 of 121
            Pseudo Genes (incomplete)         :: 68 of 121
            Pseudo Genes (internal stop)      :: 33 of 121
            Pseudo Genes (multiple problems)  :: 24 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059749.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A7214"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA7214_P2"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            1..1041
                     /locus_tag="HI844_RS14780"
                     /old_locus_tag="H4J16_014780"
     CDS             1..1041
                     /locus_tag="HI844_RS14780"
                     /old_locus_tag="H4J16_014780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_000997689.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLLK
                     DRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVN
                     KRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIA
                     KIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKE
                     DSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDA
                     DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVS
                     TENWLE"
                     /name="replication initiator protein A"
     gene            complement(1657..2337)
                     /locus_tag="HI844_RS14785"
                     /old_locus_tag="H4J16_014785"
     CDS             complement(1657..2337)
                     /locus_tag="HI844_RS14785"
                     /old_locus_tag="H4J16_014785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            2354..2830
                     /locus_tag="HI844_RS14790"
                     /old_locus_tag="H4J16_014790"
     CDS             2354..2830
                     /locus_tag="HI844_RS14790"
                     /old_locus_tag="H4J16_014790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_029756586.1"
                     /translation="MFFYYSTLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELI
                     DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKE
                     GKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
                     /name="recombinase family protein"
     gene            3044..3739
                     /gene="vanR-A"
                     /locus_tag="HI844_RS14795"
                     /old_locus_tag="H4J16_014795"
     ARG             3045..3736
                     /name="vanR-A"
                     /gene="vanR-A"
                     /gene_name="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3717..4871
                     /locus_tag="HI844_RS14800"
                     /old_locus_tag="H4J16_014800"
     CDS             3717..4871
                     /locus_tag="HI844_RS14800"
                     /old_locus_tag="H4J16_014800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="WP_002305818.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKL
                     GDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFD
                     EINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLA
                     HDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQT
                     ITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKN
                     AAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAG
                     LGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
                     /name="vancomycin resistance histidine kinase VanS-A"
     gene            complement(4969..6261)
                     /locus_tag="HI844_RS14805"
                     /old_locus_tag="H4J16_014805"
     CDS             complement(4969..6261)
                     /locus_tag="HI844_RS14805"
                     /old_locus_tag="H4J16_014805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="WP_000122610.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIYT
                     AQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADTSV
                     AKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTPLPK
                     VLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREAVEWI
                     VIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLG
                     KQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFL
                     YAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHI
                     KVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
                     /name="ISL3-like element IS1251 family transposase"
     gene            6593..7561
                     /locus_tag="HI844_RS14810"
                     /old_locus_tag="H4J16_014810"
     CDS             6593..7561
                     /locus_tag="HI844_RS14810"
                     /old_locus_tag="H4J16_014810"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="WP_001059542.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKSA
                     PFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSP
                     DSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIE
                     RLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQ
                     GAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKL
                     QRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
                     /name="D-lactate dehydrogenase VanH-A"
     gene            7554..8585
                     /gene="vanA"
                     /locus_tag="HI844_RS14815"
                     /old_locus_tag="H4J16_014815"
     ARG             7555..8582
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="D-alanine--(R)-lactate ligase VanA"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.71"
                     /coverage="100.0"
     gene            8591..9199
                     /locus_tag="HI844_RS14820"
                     /old_locus_tag="H4J16_014820"
     CDS             8591..9199
                     /locus_tag="HI844_RS14820"
                     /old_locus_tag="H4J16_014820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="WP_000402348.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTYE
                     LAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRTEM
                     ISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEAQNR
                     RRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
                     /name="D-Ala-D-Ala dipeptidase VanX-A"
     gene            9627..10538
                     /gene="vanY"
                     /locus_tag="HI844_RS14825"
                     /old_locus_tag="H4J16_014825"
     CDS             9627..10538
                     /gene="vanY"
                     /locus_tag="HI844_RS14825"
                     /old_locus_tag="H4J16_014825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="WP_000732308.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLE
                     NSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDS
                     NIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEH
                     NSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIRYVGL
                     PHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEIS
                     GNNIDGVIVTVFPGSTHTNSRR"
                     /name="vanY"
     gene            10691..11176
                     /gene="vanZ-A"
                     /locus_tag="HI844_RS14830"
                     /old_locus_tag="H4J16_014830"
     ARG             10692..11173
                     /name="vanZ-A"
                     /gene="vanZ-A"
                     /gene_name="glycopeptide resistance protein VanZ-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(11463..11792)
                     /locus_tag="HI844_RS14835"
                     /old_locus_tag="H4J16_014835"
     CDS             complement(11463..11792)
                     /locus_tag="HI844_RS14835"
                     /old_locus_tag="H4J16_014835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000754864.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIKI
                     FYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILS
                     AIRVF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            12097..12426
                     /locus_tag="HI844_RS14840"
                     /old_locus_tag="H4J16_014840"
     CDS             12097..12426
                     /locus_tag="HI844_RS14840"
                     /old_locus_tag="H4J16_014840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000629052.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEPK
                     KEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEELA
                     AEIGL"
                     /name="hypothetical protein"
     gene            12416..12703
                     /locus_tag="HI844_RS14845"
                     /old_locus_tag="H4J16_014845"
     CDS             12416..12703
                     /locus_tag="HI844_RS14845"
                     /old_locus_tag="H4J16_014845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000312842.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQK
                     TGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(12982..13545)
                     /locus_tag="HI844_RS14850"
                     /old_locus_tag="H4J16_014850"
     CDS             complement(12982..13545)
                     /locus_tag="HI844_RS14850"
                     /old_locus_tag="H4J16_014850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000017403.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAVR
                     PQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTPHG
                     RLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQVYS
                     VKEITEMTGISKATLYRYLKDNRE"
                     /name="recombinase family protein"
     gene            14188..15141
                     /locus_tag="HI844_RS14855"
                     /old_locus_tag="H4J16_014855"
     CDS             14188..15141
                     /locus_tag="HI844_RS14855"
                     /old_locus_tag="H4J16_014855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222572.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNFL
                     KCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCS
                     VSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHL
                     EGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITF
                     DCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMEFNQVNQGF
                     ISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
                     /name="IS30 family transposase"
     gene            15119..16594
                     /locus_tag="HI844_RS14860"
                     /old_locus_tag="H4J16_014860"
     CDS             15119..16594
                     /locus_tag="HI844_RS14860"
                     /old_locus_tag="H4J16_014860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="WP_000443455.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIINK
                     DGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGS
                     KRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKN
                     DENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLEN
                     LKDYKVLRYRDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKK
                     DKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFN
                     KERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMII
                     NKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKK
                     SFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
                     /name="RNA-directed DNA polymerase"
     gene            16747..17826
                     /locus_tag="HI844_RS14865"
                     /old_locus_tag="H4J16_014865"
     CDS             16747..17826
                     /locus_tag="HI844_RS14865"
                     /old_locus_tag="H4J16_014865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_002326691.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCVP
                     QTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADTDA
                     FIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDLLEK
                     LSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVLLTLV
                     ERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLHDALQD
                     VANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDYPRKILG
                     YATPHDTFVQAFRQERLTA"
                     /name="IS30 family transposase"
     gene            18321..>19547
                     /locus_tag="HI844_RS14870"
                     /old_locus_tag="H4J16_014870"
                     /pseudo=""
     CDS             18321..>19547
                     /locus_tag="HI844_RS14870"
                     /old_locus_tag="H4J16_014870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588999.1"
                     /GO_function="GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA]; GO:0009007 -
                     site-specific DNA-methyltransferase (adenine-specific)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA];
                     GO:0009307 - DNA restriction-modification system [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /name="type I restriction-modification system subunit M"
     gene            complement(19605..20285)
                     /locus_tag="HI844_RS14875"
                     /old_locus_tag="H4J16_014875"
     CDS             complement(19605..20285)
                     /locus_tag="HI844_RS14875"
                     /old_locus_tag="H4J16_014875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <20361..20549
                     /locus_tag="HI844_RS14880"
                     /old_locus_tag="H4J16_014880"
                     /pseudo=""
     CDS             <20361..20549
                     /locus_tag="HI844_RS14880"
                     /old_locus_tag="H4J16_014880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            20542..21249
                     /locus_tag="HI844_RS14885"
                     /old_locus_tag="H4J16_014885"
     CDS             20542..21249
                     /locus_tag="HI844_RS14885"
                     /old_locus_tag="H4J16_014885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002353391.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFIQ
                     RVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSEL
                     VENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLTCGT
                     ESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGK"
                     /name="hypothetical protein"
     gene            21414..21614
                     /locus_tag="HI844_RS15550"
     CDS             21414..21614
                     /locus_tag="HI844_RS15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002330566.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRFV
                     LALVSLLFKMIAAIFFIGLFR"
                     /name="hypothetical protein"
     gene            21630..21809
                     /locus_tag="HI844_RS14890"
                     /old_locus_tag="H4J16_014890"
     CDS             21630..21809
                     /locus_tag="HI844_RS14890"
                     /old_locus_tag="H4J16_014890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305761.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLLI
                     AQEKAGFDIPALKK"
                     /name="hypothetical protein"
     gene            22150..22413
                     /locus_tag="HI844_RS14895"
                     /old_locus_tag="H4J16_014895"
     CDS             22150..22413
                     /locus_tag="HI844_RS14895"
                     /old_locus_tag="H4J16_014895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287514.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKR
                     LIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
                     /name="hypothetical protein"
     gene            22407..22757
                     /locus_tag="HI844_RS14900"
                     /old_locus_tag="H4J16_014900"
     CDS             22407..22757
                     /locus_tag="HI844_RS14900"
                     /old_locus_tag="H4J16_014900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_002321032.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVT
                     VAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEELL
                     MKVRTVFQKVSK"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(22781..23395)
                     /locus_tag="HI844_RS14905"
                     /old_locus_tag="H4J16_014905"
     CDS             complement(22781..23395)
                     /locus_tag="HI844_RS14905"
                     /old_locus_tag="H4J16_014905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_010729795.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRLV
                     FQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGNPL
                     LDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQRRL
                     VYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
                     /name="recombinase family protein"
     gene            23845..25170
                     /locus_tag="HI844_RS14910"
                     /old_locus_tag="H4J16_014910"
     CDS             23845..25170
                     /locus_tag="HI844_RS14910"
                     /old_locus_tag="H4J16_014910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_000969590.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVG
                     VGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMG
                     IMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
                     /name="Y-family DNA polymerase"
     gene            25163..25513
                     /locus_tag="HI844_RS14915"
                     /old_locus_tag="H4J16_014915"
     CDS             25163..25513
                     /locus_tag="HI844_RS14915"
                     /old_locus_tag="H4J16_014915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000568378.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTEE
                     IAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIRHI
                     EFYQDNKWSRLE"
                     /name="hypothetical protein"
     gene            25510..25689
                     /locus_tag="HI844_RS14920"
                     /old_locus_tag="H4J16_014920"
     CDS             25510..25689
                     /locus_tag="HI844_RS14920"
                     /old_locus_tag="H4J16_014920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001021554.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHNFRFYIFPLYI"
                     /name="hypothetical protein"
     gene            25825..26115
                     /locus_tag="HI844_RS14925"
                     /old_locus_tag="H4J16_014925"
     CDS             25825..26115
                     /locus_tag="HI844_RS14925"
                     /old_locus_tag="H4J16_014925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000026576.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITRQ
                     RRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
                     /name="hypothetical protein"
     gene            26483..27271
                     /locus_tag="HI844_RS14930"
                     /old_locus_tag="H4J16_014930"
     CDS             26483..27271
                     /locus_tag="HI844_RS14930"
                     /old_locus_tag="H4J16_014930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000101106.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQGN
                     ATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVGSE
                     HRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKLSET
                     IETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRTITVD
                     EAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
                     /name="AAA family ATPase"
     gene            27258..27584
                     /locus_tag="HI844_RS14935"
                     /old_locus_tag="H4J16_014935"
     CDS             27258..27584
                     /locus_tag="HI844_RS14935"
                     /old_locus_tag="H4J16_014935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000796719.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKDQ
                     GSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLAKK
                     QNKI"
                     /name="hypothetical protein"
     ARG             6594..7558
                     /name="vanH-A"
                     /gene="vanH-A"
                     /gene_name="D-lactate dehydrogenase VanH-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3718..4868
                     /name="vanS-A"
                     /gene="vanS-A"
                     /gene_name="vancomycin resistance histidine kinase VanS-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9628..10535
                     /name="vanY-A"
                     /gene="vanY-A"
                     /gene_name="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             8592..9196
                     /name="vanX-A"
                     /gene="vanX-A"
                     /gene_name="D-Ala-D-Ala dipeptidase VanX-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6594..7561
                     /name="vanH gene in vanA cluster"
                     /gene="vanH gene in vanA cluster"
                     /gene_name="vanH; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3045..3739
                     /name="vanR gene in vanA cluster"
                     /gene="vanR gene in vanA cluster"
                     /gene_name="glycopeptide resistance gene cluster; vanR"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9628..10538
                     /name="vanY gene in vanA cluster"
                     /gene="vanY gene in vanA cluster"
                     /gene_name="vanY; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             10692..11176
                     /name="vanZ gene in vanA cluster"
                     /gene="vanZ gene in vanA cluster"
                     /gene_name="vanZ; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3730..4871
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.74"
                     /coverage="98.96"
     ARG             8592..9199
                     /name="vanX gene in vanA cluster"
                     /gene="vanX gene in vanA cluster"
                     /gene_name="vanX; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.5"
                     /coverage="100.0"
     Typing          2..1041
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgatt ttaattatta caaatcaaaa gaaatttatc gtgaaaaata ttatcaaatg
       61 cctaaggtat tttttactaa cgaaaaatac atggatctat caaatgatgc aaaaattgct
      121 tatatgctac ttaaagatcg atttgattat tcagtcaaaa ataactgggt agattctgat
      181 gataatatat ttttcatatt cacagttgaa gaattaatga aattgttaca atgccgcgaa
      241 ggtaaagtat ctaaaatcaa aaaggaatta gaaaaagcag gtcttctgaa acagaagaga
      301 ggaagagtaa ataaacgtga cggtaagatt gaatccatgc ctaatttatt gtacttaggc
      361 aagccagaag taacgaatca agacgtcttt aaaatcatgg aagaggaaga caatactgat
      421 accaccgtaa ttgcgaaaat cgcaaatacg gcaaaaccgc gagaaatcaa tgataccacc
      481 gtaattgcga aaatcgcaaa tacggcaaaa tccagtcata tcaaggactc aaccgtaatt
      541 gcgaaaatcg cagacaatct attctattct aattctatag acactaatag acacataata
      601 gacactgaaa ctgatcagtt acaaaatcaa gttttactag ataacttcgt cgacatcatg
      661 aaagaggaca gtataaatac atttgtacca gaaaatgtat taaacttgat aaaaacattt
      721 tctagtactt acgaagacgc tcaaaaaacc gtccaaacta ttcataatgc aaagaaaaaa
      781 gctgaagaat tagagcaaac agttgttgtt tatgaagaac tggaatctta tggaattgat
      841 gctgacaaag gattatatat gactttgctg aaagcttatc aaaaacaaaa aactgaaaaa
      901 gtaaacaatt tgcagaatct aattttcgtt tatgtaaaaa attggtttgt cgaaaaagct
      961 attgcggcaa agcttgcaaa cgaacaaaga gaaacattta acgaattacc aacagttagt
     1021 acggaaaatt ggctagagta gttgataaaa agcgatcact ataaaaatga tcaattttaa
     1081 attagaactt ttttatgatt ttaattttat tacaaatgtt acaaataaat agctattatt
     1141 ccggagtttt tagattaaaa gtttatgaaa acaatcacaa acgtaggttt taaagtcata
     1201 atcgttagat attttagtta tttttcattc aaaaaaatag ggaactagtg tttgcatatg
     1261 tgcttttttg agttataata attcatgaag agaggtaatt cacttatttc cccaagctga
     1321 aaagcctgat attacttctt ttcaggcagt gctgctaata ttcaattaca cttttttgta
     1381 tatttttagt tgttttaaat ttaattgtct gatatgcttg tgatgaattg attttcgttc
     1441 tgcacttttg gacgataaat tgtgataatt gaaaatacaa aaaaaagaaa ggcccctgta
     1501 aggctcggga gactggtaat ctccgtcagt gtcctacagg aaagcattaa gatgacgcct
     1561 taatgcgtgc ccttcagtgt tacggtatta cccgcaacgg acggttctgt tgcaaagttt
     1621 taaataaaga ataaaatccc ttacggtatc tatgatttaa gctgggattc ccaataatac
     1681 cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg ttttcttata
     1741 taatcctcga atggcttcca tgcctttaat cgtggtagag gcagtgcgta aacttcgata
     1801 gaatttattg cgtctcttta ctggacgatg gtcttgttca atcaaattat tcaggtattt
     1861 aatggtacga tgttctgtcc cttgataaaa gccgtattct tttagtttct taaaggcact
     1921 tgtaatagag ggggctttat ctgtgactac aaccttcggt tcatcaaact gcttcactaa
     1981 ccgcttaaga aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa
     2041 ggttaaacca tctgcatcga tggctcgata caaataatgc cattttcctt taattttgat
     2101 gtacgtttca tccattttcc atgaataaaa ggatttttta tttttctttt tccaaatttg
     2161 atagagtagt ttgccatatt cttgcaccca acgataaatc gtcgtatgag aaacgttaat
     2221 gccacgatca tataagattt cttgaacttc acgatagcta aggttataac gaagatagta
     2281 gcccacggct acaataatca catcctgctg aaattgcttt cctttaaaat gattcatcgt
     2341 cattcctcct gctatctttt tctattattc taccttattt gatagtagat ttaaaacttt
     2401 gcaacagaac ctacaggaag atgacatcat ttatgttaca gacttaactc gaatcactcg
     2461 tagtacacaa gatctatttg aattaatcga taacatacga gataaaaagg caagtttaaa
     2521 atcactaaaa gatacatggc ttgatttatc agaagataat ccatacagcc aattcttaat
     2581 tactgtaatg gctggtgtta accaattaga gcgagatctt attcggatga gacaacgtga
     2641 agggattgaa ttggctaaga aagaaggaaa gtttaaaggt cgattaaaga agtatcataa
     2701 aaatcacgca ggaatgaatt atgcggtaaa gctatataaa gaaggaaata tgactgtaaa
     2761 tcaaatttgt gaaattacta atgtatctag ggcttcatta tacaggaaat tatcagaagt
     2821 gaataattag ccattctgta ttccgctaat gggcaatatt tttaaagaag aaaaggaaac
     2881 tataaaatat taacagcctc ctagcgatgc cgaaaagccc tttgataaaa aaagaatcat
     2941 catcttaaga aattcttagt catttattat gtaaatgctt ataaattcgg ccctataatc
     3001 tgataaatta ttaagggcaa acttatgtga aagggtgata actatgagcg ataaaatact
     3061 tattgtggat gatgaacatg aaattgccga tttggttgaa ttatacttaa aaaacgagaa
     3121 ttatacggtt ttcaaatact ataccgccaa agaagcattg gaatgtatag acaagtctga
     3181 gattgacctt gccatattgg acatcatgct tcccggcaca agcggcctta ctatctgtca
     3241 aaaaataagg gacaagcaca cctatccgat tatcatgctg accgggaaag atacagaggt
     3301 agataaaatt acagggttaa caatcggcgc ggatgattat ataacgaagc cctttcgccc
     3361 actggagtta attgctcggg taaaggccca gttgcgccga tacaaaaaat tcagtggagt
     3421 aaaggagcag aacgaaaatg ttatcgtcca ctccggcctt gtcattaatg ttaacaccca
     3481 tgagtgttat ctgaacgaga agcagttatc ccttactccc accgagtttt caatactgcg
     3541 aatcctctgt gaaaacaagg ggaatgtggt tagctccgag ctgctatttc atgagatatg
     3601 gggcgacgaa tatttcagca agagcaacaa caccatcacc gtgcatatcc ggcatttgcg
     3661 cgaaaaaatg aacgacacca ttgataatcc gaaatatata aaaacggtat ggggggttgg
     3721 ttataaaatt gaaaaataaa aaaaacgact attccaaact agaacgaaaa ctttacatgt
     3781 atatcgttgc aattgttgtg gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc
     3841 gagggaaact tggggattgg atcttaagta ttttggaaaa caaatatgac ttaaatcacc
     3901 tggacgcgat gaaattatat caatattcca tacggaacaa tatagatatc tttatttatg
     3961 tggcgattgt cattagtatt cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat
     4021 actttgacga gataaatacc ggcattgatg tacttattca gaacgaagat aaacaaattg
     4081 agctttctgc ggaaatggat gttatggaac aaaagctcaa cacattaaaa cggactctgg
     4141 aaaagcgaga gcaggatgca aagctggccg aacaaagaaa aaatgacgtt gttatgtact
     4201 tggcgcacga tattaaaacg ccccttacat ccattatcgg ttatttgagc ctgcttgacg
     4261 aggctccaga catgccggta gatcaaaagg caaagtatgt gcatatcacg ttggacaaag
     4321 cgtatcgact cgaacagcta atcgacgagt tttttgagat tacacggtat aacctacaaa
     4381 cgataacgct aacaaaaacg cacatagacc tatactatat gctggtgcag atgaccgatg
     4441 aattttatcc tcagctttcc gcacatggaa aacaggcggt tattcacgcc cccgaggatc
     4501 tgaccgtgtc cggcgaccct gataaactcg cgagagtctt taacaacatt ttgaaaaacg
     4561 ccgctgcata cagtgaggat aacagcatca ttgacattac cgcgggcctc tccggggatg
     4621 tggtgtcaat cgaattcaag aacactggaa gcatcccaaa agataagcta gctgccatat
     4681 ttgaaaagtt ctataggctg gacaatgctc gttcttccga tacgggtggc gcgggacttg
     4741 gattggcgat tgcaaaagaa attattgttc agcatggagg gcagatttac gcggaaagca
     4801 atgataacta tacgacgttt agggtagagc ttccagcgat gccagacttg gttgataaaa
     4861 ggaggtccta agagatgtat ataattttgg ctctttgtca aatgatgttg gtaaagagaa
     4921 atcttagaat aattggagat caagtggatc agttcctctt gatctttttt agttgaaaat
     4981 caatgcgttg ccatgacgaa tcatgatgcg caatttgaaa ttataaaagc tacgaaagcc
     5041 atatgcattt cgttttaaca ctttgatatg gttgttcaga cattcaagtg ggccatttga
     5101 ataactttgt tgtaaagcat ttttgatttg tggcagaaat ttctggaacg ttctcaaggt
     5161 cgtctgatag ccttcgggaa gcatgatatt attggtaagt agttcttcaa acgatacgta
     5221 gtctcgttcc tttacagcgt aaagaaagtc ttgatataac tgatagccta cacgcaaagc
     5281 agggctccct ttcagtaagc gatccacaat atcggtctct gttaaatgag ctttgaagcc
     5341 aggtcgccaa aggcgtcgag tataatcaag cttgcgttca tctttttgaa gaagtttcca
     5401 gtatcttttt aattgcttac ctaattgata gtgtttttgt tctttgcttt ttaatagttg
     5461 gttcgtttct ttgattcgat gattgcgaaa ggtacgacca atatgttgga cgatgtgaaa
     5521 ccggtcaata atcagctgtg cttttggaaa cagcttttta acgagcgata cataaggagc
     5581 gtacatatca atcacgatcc attcgaccgc ttcacgaatt tctcgtgaaa aatgactaaa
     5641 ataacgtttt aaaaaaggca gttgtcggtt ctcgacaatg tccaataagc gctgtgtttg
     5701 tccatccatc atgatgaaac tcatagcacc tgaaacaccc cgaactgatt tgaattcatc
     5761 aaaacaaagt accttaggta acggtgtccg aaaaggattc aacggacgat aaaatcgctt
     5821 cataatacgg taaacagaag agaccgatag atttttctga gcggcgatct ctgtcattga
     5881 ggtatttttt ttcagacgag tcacgacaga ccagcggact ttctcactga tgaaacaatg
     5941 ttttttcgca acagaagtat ctgcgacaaa ggtccgctga caagctttgc atttgaagcg
     6001 ttgctttttc aattcgaggt aggtacggta ttcagaaaca tcgttcatta acaaccggac
     6061 ggttgttgta ccccaacgca caatatctga agcaatctgg tgtttacagt ggatgcattc
     6121 ttgggctgta taaatcaagt ttccacgata aatatgacag atttgccctt tgtatttttc
     6181 ttttgtgaga caattttcgt taaaagtaac tgataagtct agaatatcta atgtttcttt
     6241 gataaggtga gtgtaagaca tcttgattcc tccttttggg ttggttcgcc aaattaattc
     6301 taaaggattt caaggtgtct tttctatatg ttttggaaaa taaaaaagat gctggtgtca
     6361 tccaccaaca tcaaaaagta tagagccata attttttagg aaaatctcaa ggttatcttt
     6421 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     6481 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     6541 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     6601 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     6661 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     6721 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     6781 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     6841 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     6901 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     6961 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     7021 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     7081 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     7141 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     7201 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     7261 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     7321 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     7381 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     7441 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     7501 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     7561 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     7621 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     7681 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     7741 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     7801 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     7861 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     7921 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     7981 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     8041 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     8101 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     8161 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     8221 gtgccgcgtt agctgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     8281 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     8341 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     8401 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     8461 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     8521 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     8581 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     8641 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     8701 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     8761 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaatc gtgctgtaaa
     8821 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     8881 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     8941 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     9001 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     9061 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     9121 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     9181 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaactatata agctaactct
     9241 ttcggcagga aacccgacgt atgtaactgg ttcttaggga atttatatat agtagatagt
     9301 attgaagatg taaggcagag cgatattgcg gtcattatct gcgtgcgctg cggcaagata
     9361 gcctgataat aagactgatc gcatagaggg gtggtatttc acaccgccca ttgtcaacag
     9421 gcagttcagc ctcgttaaat tcagcatggg tatcacttat gaaaattcat ctacattggt
     9481 gataatagta aatccagtag ggcgaaataa ttgactgtaa tttacggggc aaaacggcac
     9541 aatctcaaac gagattgtgc cgtttaaggg gaagattcta gaaatatttc atacttccaa
     9601 ctatatagtt aaggaggaga ctgaaaatga agaagttgtt ttttttattg ttattgttat
     9661 tcttaatata cttaggttat gactacgtta atgaagcact gttttctcag gaaaaagtcg
     9721 aatttcaaaa ttatgatcaa aatcccaaag aacatttaga aaatagtggg acttctgaaa
     9781 atacccaaga gaaaacaatt acagaagaac aggtttatca aggaaatctg ctattaatca
     9841 atagtaaata tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat ttatctaaac
     9901 atgacgaatt aataaatgga tacgggttgc ttgatagtaa tatttatatg tcaaaagaaa
     9961 tagcacaaaa attttcagag atggtcaatg atgctgtaaa gggtggcgtt agtcatttta
    10021 ttattaatag tggctatcga gactttgatg agcaaagtgt gctttaccaa gaaatggggg
    10081 ctgagtatgc cttaccagca ggttatagtg agcataattc aggtttatca ctagatgtag
    10141 gatcaagctt gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa gaaaatgctt
    10201 ggaaatacgg gttcatttta cgttatccag aggacaaaac agagttaaca ggaattcaat
    10261 atgaactatg gcatattcgc tatgttggtt taccacatag tgcgattatg aaagaaaaga
    10321 atttcgttct cgaggaatat atggattacc taaaagaaga aaaaaccatt tctgttagtg
    10381 taaatgggga aaaatatgag atcttttatt atcctgttac taaaaatacc accattcatg
    10441 tgccgactaa tcttcgttat gagatatcag gaaacaatat agacggtgta attgtgacag
    10501 tgtttcccgg atcaacacat actaattcaa ggaggtaagg atggcggaat gaaaccaacg
    10561 aaattaatga acagcattat tgtactagca cttttggggt aacgttagct ttttaattta
    10621 aaacccacgt taactaggac attgctatac taatgataca acttaaacaa aagaattaga
    10681 ggaaattata ttgggaaaaa tattatctag aggattgcta gctttatatt tagtgacact
    10741 aatctggtta gtgttattca aattacaata caatatttta tcagtattta attatcatca
    10801 aagaagtctt aacttgactc catttactgc tactgggaat ttcagagaga tgatagataa
    10861 tgttataatc tttattccat ttggcttgct tttgaatgtc aattttaaag aaatcggatt
    10921 tttacctaag tttgcttttg tactggtttt aagtcttact tttgaaataa ttcaatttat
    10981 cttcgctatt ggagcgacag acataacaga tgtaattaca aatactgttg gaggctttct
    11041 tggactgaaa ttatatggtt taagcaataa gcatatgaat caaaaaaaat tagacagagt
    11101 tattattttt gtaggtatac ttttgctcgt attattgctc gtttaccgta cccatttaag
    11161 aataaattac gtgtaagatg tctaaatcaa gcaatctgat ctttcataca cataaagata
    11221 ttgaatgaat tggattagat ggaaaacggg atgtggggaa actcgcccgt aggtgtgaag
    11281 tgaggggaaa accggtgata aagtaaaaag cttacctaac actatagtaa caaagaaagc
    11341 ccaattatca attttagtgc tgaggaattg gtctctttaa taaatttcct taacgttgta
    11401 aatccgcatt ttcctgacgg tacccccaaa ctaagatttc atagatagat tttctgataa
    11461 ctttaaaaaa cacgaatagc ggaaaggatg tcttcattcg ctaaagagta ataaactact
    11521 tttccatctt gacgtgattt tgccattcca tgttttttta aaaaacgtaa atggtgtgac
    11581 gttgtagcga cactagcagt aagtatttct gctaaatcac agacgcacat ttccttatag
    11641 gtttctaaag catagaaaat tttaattcta gaaaaatcgc tgaaacattt ccctaacaca
    11701 agtaagtttg aaaaatcttt ttgatccaat ttatttttta ttccatttac tttttcttta
    11761 tgtacgacag tcacttggca aacttttttc attcatctca cctcacattc aaacgattat
    11821 ttgaatgaag tatacctatt tgatcactct tttgtcaatc taccttaatt ccaatacact
    11881 attataagca atttcatgta ttgaagtaaa cttacttgca agtgcgtttt tgattccaag
    11941 ttaaaaacat attattattc tacataattt cctaatcaaa taaaactcct ttgaaaattg
    12001 ttctaaagga gttttattat tttgtatttc tactaaaaat agcgtacaat gtaattacta
    12061 agtaattacg aaatgtaaaa taatggagga aataatatga ttacaaaaac aagaaaacaa
    12121 ggaaattcta ttatgttaac ggtccctaaa gaattcgatg tgcccaatgg tgttgaagta
    12181 gaagctaagc ttgtagagaa tggtatcctt tatgagtttg tagagcctaa aaaagaattt
    12241 tttgatttta gtgaagatat tttggctgat attctttcag aaggttatag caaacaagag
    12301 atcttaaaag aatttagaaa tagaaaaaat gcgttaactt ctgcatttaa atctattgct
    12361 gaagatactg ttgcaaattc taaaccgatg acaaaagagg agttggcagc agaaattggc
    12421 ttatgagatt ctaccttcta aacacgtaat aaagtatcta aaaaaattaa aagagaaaac
    12481 attaaaagaa caatttttaa cgataattta tgatgagata gcagttcgtc ctcattcggg
    12541 agaacaaaaa acgggtgatt tatcaggaat ttgggctatt ggattcaagt atgcaggtac
    12601 gacatatcgc gttgcatatg aaattaaaga taatgcagtg atacctaact tactatgtgg
    12661 aacgcatgaa aatttttatg aacaattaaa aaagattagg taagaaccca atgtttcaac
    12721 ttgagaggat agacgacact ttaaaagata atttttaaca gctgagagtt gtaataccct
    12781 ataattttaa accactaacc tatatattta tgaaaaatag aaaaaattaa aacgcatttt
    12841 aagcctcttt caaattaaaa gcttcatcct tatgcacaaa gagggcttaa aatgcgttat
    12901 tttatattgt tttttagtaa taataaaaaa atcactctaa ttctcccata aatattatag
    12961 attcctaaag attaaaatag attattccct attgtctttt aagtagcggt agagtgttgc
    13021 cttgctaata ccagtcattt ccgtaatttc ttttacgcta taaacctgac ttttatacaa
    13081 ttttactgct cgatcaacat ctttctggtt cactcgtgga cggccacctt tacgacctct
    13141 cgcacgtgca cttttcaaac cttctttggt acgttcaaca attaaatcac gttcgaactg
    13201 actaaatgct tggaagacag tcatcattaa gcgtccgtga ggcgtgctgg tatcgaagtt
    13261 ttctttcaag ctcaccagct taacatggtg ttcttcaaaa taggtaacaa ggtcaatcaa
    13321 atcctttgta ctacgaccca atcgagaaaa actttctaca attaccgtat ctcccggacg
    13381 aattttatct tttaaacgat ttagttgggg acgaaccgct ttggttcccg tcattttctc
    13441 agttaggatt tcattacaat tttgttcatt gagtaagtct aattgacgat ttagttcttg
    13501 tgaacgtgta ctcactcggg cataaccata aatgtatcca ctcatgcttt tcttcctttc
    13561 gtactttaat tctggcttga ttgtatcata aaagggagtt atagatacat agttaatgca
    13621 acaagttatg atacaaaata tgacctcttt tatttaataa aagaggtcat ttcattttgt
    13681 ctcataatcg attgtttttg atacgagtgt gtatacgaaa taaacaaaaa aatcttaagt
    13741 aaatttctag atacctaatt taatcctaac tagtgtatcc taaagttaat aacgagttta
    13801 tcacaatgaa aattataaaa tgctcccata ttagatataa catgcaatat ttgaattaga
    13861 actacaaaat ttattcgttt tataaagtaa ataatgttga gatactaagg aatagaatat
    13921 tataaggaat tgagctgaat gtattggata aaaaaagaat aactttatta gagatggact
    13981 ataaaaatgc tcgtaatttt cttctgaagt ctgaaagcta tttgaatgct cagctaccag
    14041 aatatattag ttttgacgaa actttaaaat ctgctaaaaa agttttaact tcgccgtttg
    14101 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaattg taatacccgc
    14161 taaagcatac aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt
    14221 gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt
    14281 aaacgtggac gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca
    14341 cttgaatact ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt
    14401 tttcctgctg aggaaaaaga atacattgaa aaaagatcaa ctgaaggttg gaccccagac
    14461 gtcattattg gccgtgcaga gcgaaccttc tcttgttcag taagtaccct ctatcgccgg
    14521 tttaagacgg gagaattcaa tgttttacat ttaccgatgc aaggaaaacg aaaaccaaat
    14581 ggttataagg aaaaacgtgg aaaacaggca ttcaaaagaa atatttctga acgtaaaaaa
    14641 gattatgtcg ttttcgaaga agagtttgga catttagagg gtgatacgat tgtcggcatc
    14701 caccataaaa gtgccgtcat cacactcgtt gagcgacttt caaaagccat tatcgccttg
    14761 aaaccagaag gccgtaaggc agttgatatt gaaaattcga ttaatgaatg gcttcaatcc
    14821 gtacccaaaa atcttttcaa atcaattacc tttgattgtg gaaaagaatt ctctaattgg
    14881 aaaagtatta gtaatacgaa tgatattgat atttatttcg cagacccagg aacgccttcc
    14941 caacggggat taaatgaaca ttcaaacgga ctcctccgaa aagatggact accaaaagaa
    15001 atggaattca accaagtcaa tcaaggattc atctcatccg ttgcgtccaa aagaaaccat
    15061 atccctagaa aatcactaaa ttaccaaaca ccattagaag tttttttgag ttacgtaaat
    15121 ggaaagtttt gtctcgctta atttgacaaa taatacttca aaaaaaaacc aaccgaaaga
    15181 tataaaagat attgtagata cacgaatgta taaagaacgt gatgacttaa actatacgct
    15241 tattatcaat aaagatggaa actattcatg gcgtcctctc gttctactac atcctattct
    15301 atatgtagat ttggttaatt gtctcactaa agaagaccat tgggaggagt taaaaaagag
    15361 gtttagtgaa tttaaaaaag ataaaaatat taactgttat tctatacctg ttgaagcttc
    15421 caaaggttca aaaaggacag atcttggtga aacaatacta aactggtggg aatattttga
    15481 acaaaaaagc ttagctcaga atattaaata ccaatattgc atgtttactg acatcgctga
    15541 ttgttatcca tcaatttata ctcactctat aacatgggcc atgtatggta aagaaaaagt
    15601 taaaaatgat gaaaataagt gttcgggctc acttggcgat aaaatagata aaaaaatatc
    15661 tggaatgcaa cacaatcaaa caaatggtat ccctcagggt tccgttctta tggattttgt
    15721 agcagagatc gttctagggt atgttgattt gagattatcg aaagaattaa aaaaattaga
    15781 aaatttaaaa gattacaaag tactcagata tagagatgat tatagaatat tttcaaataa
    15841 tcgtaatgat ttggaacaaa ttatgaaagt gttatcagaa gtcttatatg atttaaattt
    15901 taagttaaat tctagtaaaa caaaattatc agatgatatt atcatagatt ctatcaaaaa
    15961 agataagctc tattgggaag aaattaaggc aagtataaga atgaatgcca caaacttaga
    16021 tggtgataga gaaattcgtt atagtataaa tttacaaaaa catttattgg aaataaaaaa
    16081 gttagcagat aagcatccta attcaggcca attgaaaaaa gcactttctg agtttaataa
    16141 agaacgcatt attaaaatca cttcaaagcc gaacgatctt ttaccactta ttggtatcct
    16201 ggtagatatc atgataaata acccgggttc tttgccgcag tgtgtcttaa ttatagggca
    16261 gttattaaat ttcgaaaatg agagtaaggc tagagaaata gttgaaatga ttataaacaa
    16321 atatgcttta aagccaaata ctgaatttat gatgatatgg tttcaaagat tgaccaccat
    16381 ttttggagaa atgtttgata gtgttttatc atccgaattt gtatctaaaa tcaaagatcc
    16441 tgtctctaat cagatgtttt ctgtagactg gctaaatgaa gatgctaaga aatcttttta
    16501 tgaagggacc attaaagacg aagagaaata tattaaaatg agtaagaaaa tatatattac
    16561 tgaatttgat gattttaatg attatccttt ttaagtgaaa atttagaatg aatataaaaa
    16621 acattacaat ctaatttttt ggcggatttt ataattaact tagccatttg gaaataaaaa
    16681 aacgccacgt gaattccatg ttatattgaa gttaccacaa ctctcaatag acaaggatga
    16741 attcacatga cgcaaaacca tgataacaca gtttcacgca agggaaaaca cctaacctac
    16801 tcagaacgtt gccaaatcgc catcctgaaa aaggaaaact attccaaccg tcaggttgcg
    16861 gatgtcctgg gctgcgtgcc gcagaccata aacaacgaaa tcaaacgcgg gaccgttact
    16921 cagcttaacc gccagaatca gaacgggaag gtctacgact attatcatga cgtctacgac
    16981 gccgatgccg ggcaggcggc ctacgagaag aaccgcttga actgcggcag acggccctta
    17041 tgggccgaca cggacgcctt cattgaatgg gcggacgaca tgatgctgtt ggaaaaatgg
    17101 tccccggacg tggtggtggg ctttgccaga aaacacgaat tgttcgaccc ggccattatc
    17161 cctgggacca ccaccctcta tcagtggatc gaccgtggca tcatgaaaac gaagaatatg
    17221 gacctgctag aaaaactgtc ccgcaaaccg aaatcgtccc gtcatggcaa ccgcccgaat
    17281 aaacgggtgc tagggaaatc cattgaagag cgcccggagg cggttggtac ccggaaaacc
    17341 ttcggccatt gggaagtcga cacggtcgta ggaaacaaga ccaagtcgga tgccgtgctg
    17401 ctgacgttgg tggaacggca gacacgcttc gaggtcattc tgaagctggc cgggaaggat
    17461 gccgaatcgg tcaaccaagc ggtccgacag ctgagagaac gggctggtgc gtacttcggc
    17521 cagctgttca agacggttac ctcggacaac ggctcggagt tcgcgggact gcacgatgcc
    17581 ttgcaggatg tggcaaacat ctacttcagc cacccctatg cttcctggga gcgcgggacg
    17641 agcgaaaacc aacacaagct gattcgccgt ttcatcccca agggcagccc aatcggcaac
    17701 gtgagcgaaa accaaatctt gcggattcag cgttggatga acgattaccc gcgaaagatt
    17761 ttgggctatg caacgccgca tgacacgttc gtccaggcat ttaggcagga acggttgacg
    17821 gcttgacacg tgggcccttg acaacgagcc tccctcggca tggcttgtgt ctggaaagct
    17881 ggccggggcc agccagtcgg gagactacca tgcctcagct cattgtcaag ggcccactcg
    17941 attagaaaaa ttagacggtt ggtggtaacg agcaaccggg tcaccatggg tggctaactt
    18001 aaacttgaaa tttacgaatc taattttttt aaaaaaatgt tatatcggaa gtaatctctt
    18061 gatttctata ctataagata agaaaaccac tatttaacaa ggttattcga accttatttt
    18121 aagttactta ttatttaatg tatcgaaaaa ggtgaaatag gtttgttata ataagaatta
    18181 agtaagacat gaattgtcta agcaaatgta gcttatccat ttcattaaaa tacatatggt
    18241 gaaaaaacaa gttcaaatag taactaattt tttaaaaaac tgatttataa aaaatacaac
    18301 acgaatgagg aaaataacca atgacagaac aaatcgaaaa aaattcaaaa agcttatatc
    18361 aggcactgtg gaattccgca gatatcctgc gttcaaaaat ggacgctaat gaatacaaga
    18421 gctatttact aggcttggtc ttttataaat acttatccga caacatgctg cggtatgtct
    18481 ccgttctatt ggaggaagaa acggacgacc taaataaagc acaagacctt tacgttgaag
    18541 catttaaaga tgcagatata aaggatgatc tcttgtatga actaaaagat gagttttcat
    18601 ataccatcgc acccgcattg acctttaccg cgcaagtggc agccattcat gatggatctt
    18661 ttcaattaga agacctggta caaggtttcc gtgacattga acaatccagc gaaaactttg
    18721 agaacttatt tgaagacatt gatctgtatt ctaaaaaact ggggtcaacc ccacaaaaac
    18781 aaaataaaac catcgcagat gttatgaaag aattagaagg gttaaacatg gcggggcatg
    18841 ctggcgatgt cttaggcgat gcctatgagt acttaattgg ccaattcgca tctgaatccg
    18901 gtaaaaaagc cggtgaattc tataccccac aaccggtcgc aaaattgatg acacaaattg
    18961 ttttacaagg aaaagaagat aaaaaaggct tttctgttta cgacgcgacc atgggttcgg
    19021 gctcgttact tttgaacgct aaaaaatatt cccatcaacc gggaaccatt tcttatttcg
    19081 gacaagagtt gaatacgtct acctataatt tggcgcgtat gaacatgatt ttgcatgggg
    19141 ttcccattgc caaccaacac ttgcataatg cggatacctt ggatcaagac tggccaacag
    19201 aagagccaac gaacttcgat ggggtgctca tgaacccacc gtattctgca aaatggtcgg
    19261 ctgataaagg cttcttagat gacccacgct tctctgccta tggcgtatta gcccctaaat
    19321 caaaagcgga ctttgctttc ttattacatg gctactatca tttgaaagat acgggcgtca
    19381 tggccattgt tttgccacat ggggtgctct ttagaggggg cgcagaaggg aagatacgca
    19441 aagccttgtt agaaaatggt gccattgaca ccgtcattgg cttacctgct aacatcttct
    19501 ttaatacgtc cattccaacg accgtaatta tcttgaagaa agaccgtgcg ggttctgttg
    19561 caaagtttta aataaagaat aaaatccctt acggtatcta tgatttaagc tgggattccc
    19621 aataatacct tgatttcagt acagaccgaa aacccgaaga gagtgccttc ttttcgggtt
    19681 ttcttatata atcctcgaat ggcttccatg cctttaatcg tggtagaggc agtgcgtaaa
    19741 cttcgataga atttattgcg tctctttact ggacgatggt cttgttcaat caaattattc
    19801 aggtatttaa tggtacgatg ttctgtccct tgataaaagc cgtattcttt tagtttctta
    19861 aaggcacttg taatagaggg ggctttatct gtgactacaa ccttcggttc atcaaactgc
    19921 ttcactaacc gcttaagaaa agcataggct gcttgtgtgt cccgtttttt acgtaaccaa
    19981 atatccaagg ttaaaccatc tgcatcgatg gctcgataca aataatgcca ttttccttta
    20041 attttgatgt acgtttcatc cattttccat gaataaaagg attttttatt tttctttttc
    20101 caaatttgat agagtagttt gccatattct tgcacccaac gataaatcgt cgtatgagaa
    20161 acgttaatgc cacgatcata taagatttct tgaacttcac gatagctaag gttataacga
    20221 agatagtagc ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga
    20281 ttcatcgtca ttcctcctgc tatctttttc tattattcta ccttatttga tagtagattt
    20341 aaaactttgc aacagaaccc ttggaagtag gtgtcaaacc aagcactatc aaaccttccg
    20401 aatatcaagc cttagaactg acttatgcaa aattcgcaca gattaagaaa gctaaagcaa
    20461 tggctagcga ctggctagat acttttgaaa gtatcgaaaa aaatggtgca ttttatacga
    20521 ttgaatcgga ggagaaagat aatgaataag gtggtttatc agctaacaac agaagatgtt
    20581 tcgcaatttt taacaagcaa agaacacttg aatgaagtaa tcagacggat tcaaaaagac
    20641 tttagttgta tggaagaaat gaagtacttt attcaaagag tggaagaaca gttgtatatg
    20701 gaaagcgaaa caagtcaaac gaaggtcaca gtcagccata atcatttaga cgtgtataaa
    20761 cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac gactagaaac agaaaaatac
    20821 atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa actttgtcag ctacacgatt
    20881 tgtaggaaac actattactc agaatcagaa tattattcaa ttttcgagag catggaatta
    20941 atggaagaag aaatggaaga gctaaacagc aaaaatgcga cggtagacga ttattacctt
    21001 gtatttacta ggtttttgac gtgtgggacg gaatctatcg tgttaatggg aatggattat
    21061 gaactcgctg tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc
    21121 gacgactggt acacgacttt aactgatttt ttagacacct acacttacga cgatacagaa
    21181 gaaatccaaa atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa
    21241 ggtaaataac catttatacc gaaaagtgag gatgtgtgaa ataagaacaa aaaaccttga
    21301 aattaaggga atggttctta tttcgcacca atcgctccaa ccgcccccgc cgcttaggtg
    21361 gtgcgaaata agcgggcaaa gcccgataac ctaagagaaa gaaggagatc catatgaata
    21421 aaatcattcc tctattgttc gtactgtttg ttggcgagat acttatgcgt catgctgcag
    21481 ctgtacattt aaacttcgta gtattcttag gaatactggt attttgtttg atcttttggc
    21541 gatttgtatt agcccttgtg agtctactat tcaagatgat tgcagccata tttttcatag
    21601 ggttatttcg atgaaaggag agagtactaa tgccaattgc aataggaaat aaacggttac
    21661 ccgtgacatt agatgaaaag agacaaaaag aattgcagca actaaagcag aagtacggaa
    21721 aaagtgaatc taagattatg tgtattgcgt tagatctatt gatcgcgcaa gaaaaagcag
    21781 gatttgatat accagcacta aaaaagtgac gtcacttttc atttgaagaa acaaaagtga
    21841 tagcacttaa ttgttcctac tatttagaat attttctatg tcctttttat tccattttgt
    21901 atcaataact atgaaatctt taaagtatcc agaagagatt atttttagat caattttttt
    21961 cttacctgat tctaaagatt ttgttttctt aaacatatta tgatttcctt tcgactattt
    22021 tcatataaat gttaacatag tttatggatt catggaatgt taatctaatt taatagaaaa
    22081 ggataattaa gtatatacgt tttttcttat gtatgttata ctatgtatat acagaaagag
    22141 gtgtctacta tggaaatgtt acctataaaa aaatggggaa atagtaatgg ccttagattg
    22201 ccaaagtata ttatggaata tcttggaatt catacagaag acaaagtgaa gataatacaa
    22261 gaagaagtaa acggtcagaa gcggctaatt atcgaagcgg ctaatataga aaatgaatta
    22321 acaattgaac agttatttga aaattacact gatgaaagaa atcatgtgac gattcaaaat
    22381 ttaggtgaag cagtaggtaa tgaaaaatgg tgagaaaacc tagacaaggc gatattctat
    22441 tattgaatac agcacctcga tctggacacg aacagacagg taaaagacct tatattgttt
    22501 taagtcatga tattattgcg gattatagta acgttgttac agttgctcca atttctagta
    22561 caactagaaa ctatccgcta tatgtaaata taaactcaga atatgaaatg aagacaacag
    22621 gaaaagtatt attagatcaa ctaacgacaa ttgattatga agccagagag tgtatttttt
    22681 tggaaaaagc gcatgatagt ttagttgagg agttattgat gaaagttaga acagtttttc
    22741 aaaaagtaag caaataaaat agaaacagca gcatgatcaa ctattcgtga ttgaccatgc
    22801 tgtctttttt tattctatat acggtttgtc tcgtaacatt gtagtctttt gcaatcttag
    22861 cgatcgcaac accttttttt agatcctcaa cgatgttttt ataaacaaga cgacgttggg
    22921 gatcttttgc attggcactg tatagcttag gacgtccttt ataaacaccg ttcgcttttg
    22981 caatcttaat gccttgtgcc tgacgtcgtt tagattcggt acgttcttgt tcagcgatca
    23041 tggccaaaat ttgaatgatc aaatctttaa tgaatttatc caggagagga ttgccaattg
    23101 cttcggccat gatcggtaga cttgtgatga tcagctgcac atttttcttc ttcaagtaat
    23161 tgactgaagc gatgatctcg tcgtaatttc ttccaagtcg atcaatagct tccacaataa
    23221 aaatatcttg atctcggacg aaatccagtg cttcttgaaa aacaagtcga tgagtaatcg
    23281 ttgcgccaga ttgtttctca atgaaaattc tctctgcacc gaattttttc atttcttcaa
    23341 tctgccgttg ttcattttga tcgatggatg agacacgtat gtatgcaact ctcaaaattt
    23401 ttacctactt tctgcaacta tttaaaaaac accctaatca tacacgagaa aaccagattt
    23461 ttcaatgtcg cggtcgtgtc tagttagggt aaaccttata tagacaaaaa ctaatgatgg
    23521 aggaaatagt atgagaattt tttgtaagtg tctactgata acttattctc aaatgttgta
    23581 aggattttat tcatttgtta atattaaaga acggaactcc aaaaaagtcc gtactctaat
    23641 ttgcagtctc tcactctttt agagtgagag tttttattag attttcaaat taattagtaa
    23701 agtgatagaa taaattgatc atacaaatca aaagaagaag cagggtgata gcatacctat
    23761 gcttcttttt ttatgtattt agctatttct tctttacaat atcgaacata tgtttgtata
    23821 atgtgaaaga atggagtgtt atttatgatg gattattcaa aagagccagt aaatgattac
    23881 tttttaatcg atatgaaaag cttttacgca agtgtcgaat gtatagaaag aaatttagat
    23941 ccattaacaa cagaacttgt tgttatgagt cgagctgata atactggttc aggattgata
    24001 ttagcttctt ctcctgaagc aaaaaagcgg tatggtatta caaatgtgag tagaccacgt
    24061 gacttaccac aaccatttcc taaaacacta cacgttgttc caccacgtat gaacctatat
    24121 atcaagcgaa atatgcaggt aaataatatt ttcagaagat atgtggctga tgaagatcta
    24181 ctgatttact cgatcgatga atcaatctta aaagtgaccc gatcactgaa tctttttacg
    24241 actgaaggaa cacgaagtca acggagaaag aagctggctc aaatgatcca ggagaatatt
    24301 aaagaagagc taggattgat tgctacagta ggtgtcggag ataatcccct tttggctaaa
    24361 ctagccttag acaacgaagc aaagcataat gaaggatttg ttgcggaatg gacgtacgaa
    24421 aacgttcctg aaaaagtatg gaatattcct gaaatgacgg acttttgggg aattggctct
    24481 cgaatgaaaa aaagactcaa tcagatgggg attatgagca ttagagatct tgctaactgg
    24541 aatccttata cgattaagaa tagactaggt gtgattggct tacagctgta cttccatgct
    24601 aatgggatcg atcgaacaga tatagcaatc ccaccagaac cgacaaagga aaaatcatat
    24661 ggaaattcac aagtgttacc acgagattat actagaagaa atgaaatcga gctggtggta
    24721 aaagaaatgg cggaacaagt agcgatacgc attaggcagc ataactgtaa aacaggttgt
    24781 gtccatttac acattggcac gtcgatctta gaaacgagac cagggttttc acaccagatg
    24841 aagatcccta tcacagataa tacaaaggaa ttgcagaatt attgtctttt cttgtttgat
    24901 aagtattatg aaggacaaga agtaaggcat gttggaataa cctatagcaa gcttgtgtac
    24961 accgattcgc ttcagctgga tttatttagt gatccacaaa agcaaattga cgaagaaaat
    25021 ttagataaaa ttattgacaa gattcggcaa aaatatggtt ttacgtcgat tgtacatgct
    25081 tcttcaatgt tagaaggcgc tagaagcatt actagaagca cacttgttgg tggtcatgcg
    25141 ggcggaaatg ggggaattaa aaatgattga ttacaatgat cgtggacgtt taaagtgggc
    25201 agggttttat ttatctgatc atactgaaga aattgatgcc gacaatgaac aacgaaccag
    25261 tcaaaattta gcaaaagaac aaatgacgac tgaagagatt gcggaagtat tgaacgctgc
    25321 agtcataaaa aatagatcag tatctattca aaaagaagaa cgaaacgctg aaggatacta
    25381 tccagaagat atcattggca agatcttagg tagtgatgag ctggggatct acgttggaaa
    25441 agacaaaatt cactacgacg aaatacgtca tatcgaattt taccaggata ataaatggag
    25501 tcgattagaa tgaacattat ccagcaatat gagctaaagt atataacctt tgatcagcta
    25561 tcagaagaga tttggggtta tggccaacgt ttgataaatg aggttggtgt tgagcgcttc
    25621 tctttctatg tagaagctgc agcaggttat cataatttta ggttttatat cttcccgtta
    25681 tatatataag gtattttacg gattctatag agttatctct atgaatcctt ttttgttttt
    25741 acaaatagga gtagatacta gaataatgcg tgtgttaaac tattattgca aacgcaataa
    25801 taaaagaaag taggcttacg caccatgtct atcaatacat ttgtttactc gcacccgatc
    25861 aatgtttaca ttatcaagaa tttaggaatc actgtagaac aattttgtga gttatatgcc
    25921 tacccacaag gaacggttgc aagctggata acccgacaac gtagaataaa atcattacct
    25981 gcttcattcg tttatgattt aagcctggcc tctagtttaa acatgtctga tgtttatgaa
    26041 aagctcctgt ctttagaaaa agaatatgat tccttcaaga ttaaacatga taaaaaaata
    26101 aaaaaacata tataatatca atcacaattg tataatatta cgatatctta atatcatgat
    26161 atcgtaatat tgtttttttc taagaaaatg cactataaga agggaattat tagtaacaat
    26221 atagacagtt tagtaatgtt taaattattc tagttgctgc tcgcaaaaca tatttcttat
    26281 cattaataaa aaattttaaa gtaataaaaa gtgattattt taatttcgaa taacatatga
    26341 ataatcttta tatccgaacc gatatatatc tttaaaacaa aataatcaca caatattaca
    26401 atataatgca tatacgatat tgcaatatca caatattatg atataattat attataatat
    26461 aagatgaatg ggggatattt taatggcaag aatattgtca gtagccaatc aaaaaggtgg
    26521 agtttctaaa actacgacta gcgttaattt agcagcatgt ttgaaatttg tttacggtaa
    26581 aaatgtactt ttagtggaca ttgatcctca gggaaatgct acagataatg taggtttcga
    26641 tatagatgga acaaaccaac ctacaattta tgaagtatta aaaggggagg ctgacattac
    26701 agatgctatt ctggactata aaggaattga tgttttgcca gccgacatag ccctttcttc
    26761 tgcagagagg gaatttactc aagtaggaag tgaacacagg cttaaaagag tacttcaacc
    26821 aatcaaggac aactatgatt atattataat tgattgtcca ccaagtttag gaatgcttac
    26881 agtcaacgca tttactgttt ctgatgaaat tatcattcca gttgaagctg cgtatttctc
    26941 attaaaagga ctaataaagt taagtgaaac tattgaaacg gtaaaagaat atacgaacga
    27001 caagcttgaa gtacgaggag ttctatttac taagtataat aagcgttata acatttctaa
    27061 agaaatgacg tctgcagcaa ccgaaatttc tagagtcatt ggtgcaaaaa ttttcaaaac
    27121 ttatattcgt aggacaataa ccgttgacga agcgcaagct gctggatcag atctaatcaa
    27181 ttttaataaa ccctcaacag ctgaagatga ctataaagca tttacagaag aatatttaaa
    27241 agaagttggt gattttgatg gcagaagata atcgcagtca agctgcagta agtagattac
    27301 taggaaataa aaataaaaag aattttaaat caaaagaagt agaagaaatc gaattaacca
    27361 agaaaaatat aagtaaagtc ataaaagatc aaggaagtag tgtaagaaca gcagatagca
    27421 ttcgtataaa tccaattcta atgtcagcac tcaaatattg gacgactatt gctgaatcag
    27481 acaaaagtaa accagacgtt atagaagatg cgctattaaa gtccatacct gaagaatact
    27541 tgattgaagg atataaacta gcaaagaaac aaaataaaat ttaatttaca ttcaatatca
    27601 cgatatcata atatcgtgat atttttttta taaaaatcag tagtatcaaa gctgataagt
    27661 cgcttatctt catattgtaa tattacgata tcgtgatata atattattgt aatatcgtga
    27721 tatagttact atgagcttaa accttgaaat agagcggttt gctttaattt attttttaag
    27781 gtaaactaaa aaaagataaa aaaagactac tgctttaaca aagtttggcg acttatcagc
    27841 agtagaaaaa tatatagtgg atagatatat ttgtttaatt cattgtaaca tagttttgaa
    27901 caaaacgcta tcctacagaa agaggattgg ct
//
