LOCUS       NZ_CP059742            28489 bp    DNA     circular CON 01-OCT-2023
DEFINITION  Enterococcus faecium strain A4694 plasmid pA4694_P3, complete
            sequence.
ACCESSION   NZ_CP059742
VERSION     NZ_CP059742.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14675385
            Assembly: GCF_012933245.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28489)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Complete genome of vancomycin resistant Enterococcus faecium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28489)
  AUTHORS   Veeraraghavan,B., Vasudevan,K., Arumugam,A., Solaimalai,D. and
            Bakthavatchalam,Y.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 28489)
  AUTHORS   Veeraraghavan,B., Solaimalai,D., Vasudevan,K. and Arumugam,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Department of Clinical Microbiology,
            Christian Medical College and Hospital, Ida Scudder Road, Vellore,
            Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: CANU v. v. 1.7; UNICYCLER HYBRID v. v. 0.4.6
            Assembly Name                     :: A4694
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91.24x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/01/2023 03:42:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,020
            CDSs (total)                      :: 2,928
            Genes (coding)                    :: 2,815
            CDSs (with protein)               :: 2,815
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 38 of 113
            Pseudo Genes (incomplete)         :: 69 of 113
            Pseudo Genes (internal stop)      :: 30 of 113
            Pseudo Genes (multiple problems)  :: 20 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059742.1.
            Bacteria and source DNA available from Christian Medical College.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..28489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="A4694"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pA4694_P3"
                     /geo_loc_name="India:Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="CMC Vellore"
     gene            1..1041
                     /locus_tag="HI848_RS14475"
                     /old_locus_tag="H4J15_014475"
     CDS             1..1041
                     /locus_tag="HI848_RS14475"
                     /old_locus_tag="H4J15_014475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_000997689.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLLK
                     DRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVN
                     KRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIA
                     KIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKE
                     DSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDA
                     DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVS
                     TENWLE"
                     /name="replication initiator protein A"
     gene            complement(1657..>2157)
                     /locus_tag="HI848_RS14480"
                     /old_locus_tag="H4J15_014480"
                     /pseudo=""
     CDS             complement(1657..>2157)
                     /locus_tag="HI848_RS14480"
                     /old_locus_tag="H4J15_014480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(2214..2894)
                     /locus_tag="HI848_RS14485"
                     /old_locus_tag="H4J15_014485"
     CDS             complement(2214..2894)
                     /locus_tag="HI848_RS14485"
                     /old_locus_tag="H4J15_014485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            2911..3387
                     /locus_tag="HI848_RS14490"
                     /old_locus_tag="H4J15_014490"
     CDS             2911..3387
                     /locus_tag="HI848_RS14490"
                     /old_locus_tag="H4J15_014490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_029756586.1"
                     /translation="MFFYYSTLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELI
                     DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKE
                     GKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
                     /name="recombinase family protein"
     gene            3601..4296
                     /gene="vanR-A"
                     /locus_tag="HI848_RS14495"
                     /old_locus_tag="H4J15_014495"
     ARG             3602..4293
                     /name="vanR-A"
                     /gene="vanR-A"
                     /gene_name="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4274..5428
                     /locus_tag="HI848_RS14500"
                     /old_locus_tag="H4J15_014500"
     CDS             4274..5428
                     /locus_tag="HI848_RS14500"
                     /old_locus_tag="H4J15_014500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="WP_002305818.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKL
                     GDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFD
                     EINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLA
                     HDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQT
                     ITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKN
                     AAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAG
                     LGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
                     /name="vancomycin resistance histidine kinase VanS-A"
     gene            complement(5526..6818)
                     /locus_tag="HI848_RS14505"
                     /old_locus_tag="H4J15_014505"
     CDS             complement(5526..6818)
                     /locus_tag="HI848_RS14505"
                     /old_locus_tag="H4J15_014505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="WP_000122610.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIYT
                     AQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADTSV
                     AKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTPLPK
                     VLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREAVEWI
                     VIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLG
                     KQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFL
                     YAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHI
                     KVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
                     /name="ISL3-like element IS1251 family transposase"
     gene            7150..8118
                     /locus_tag="HI848_RS14510"
                     /old_locus_tag="H4J15_014510"
     CDS             7150..8118
                     /locus_tag="HI848_RS14510"
                     /old_locus_tag="H4J15_014510"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="WP_001059542.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKSA
                     PFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSP
                     DSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIE
                     RLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQ
                     GAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKL
                     QRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
                     /name="D-lactate dehydrogenase VanH-A"
     gene            8111..9142
                     /gene="vanA"
                     /locus_tag="HI848_RS14515"
                     /old_locus_tag="H4J15_014515"
     ARG             8112..9139
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="D-alanine--(R)-lactate ligase VanA"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.71"
                     /coverage="100.0"
     gene            9148..9756
                     /locus_tag="HI848_RS14520"
                     /old_locus_tag="H4J15_014520"
     CDS             9148..9756
                     /locus_tag="HI848_RS14520"
                     /old_locus_tag="H4J15_014520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="WP_000402348.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTYE
                     LAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRTEM
                     ISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEAQNR
                     RRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
                     /name="D-Ala-D-Ala dipeptidase VanX-A"
     gene            10184..11095
                     /gene="vanY"
                     /locus_tag="HI848_RS14525"
                     /old_locus_tag="H4J15_014525"
     CDS             10184..11095
                     /gene="vanY"
                     /locus_tag="HI848_RS14525"
                     /old_locus_tag="H4J15_014525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="WP_000732308.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLE
                     NSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDS
                     NIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEH
                     NSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIRYVGL
                     PHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEIS
                     GNNIDGVIVTVFPGSTHTNSRR"
                     /name="vanY"
     gene            11248..11733
                     /gene="vanZ-A"
                     /locus_tag="HI848_RS14530"
                     /old_locus_tag="H4J15_014530"
     ARG             11249..11730
                     /name="vanZ-A"
                     /gene="vanZ-A"
                     /gene_name="glycopeptide resistance protein VanZ-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(12020..12349)
                     /locus_tag="HI848_RS14535"
                     /old_locus_tag="H4J15_014535"
     CDS             complement(12020..12349)
                     /locus_tag="HI848_RS14535"
                     /old_locus_tag="H4J15_014535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000754864.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIKI
                     FYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILS
                     AIRVF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            12654..12983
                     /locus_tag="HI848_RS14540"
                     /old_locus_tag="H4J15_014540"
     CDS             12654..12983
                     /locus_tag="HI848_RS14540"
                     /old_locus_tag="H4J15_014540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000629052.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEPK
                     KEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEELA
                     AEIGL"
                     /name="hypothetical protein"
     gene            12973..13260
                     /locus_tag="HI848_RS14545"
                     /old_locus_tag="H4J15_014545"
     CDS             12973..13260
                     /locus_tag="HI848_RS14545"
                     /old_locus_tag="H4J15_014545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000312842.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQK
                     TGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(13539..14102)
                     /locus_tag="HI848_RS14550"
                     /old_locus_tag="H4J15_014550"
     CDS             complement(13539..14102)
                     /locus_tag="HI848_RS14550"
                     /old_locus_tag="H4J15_014550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000017403.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAVR
                     PQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTPHG
                     RLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQVYS
                     VKEITEMTGISKATLYRYLKDNRE"
                     /name="recombinase family protein"
     gene            14745..15698
                     /locus_tag="HI848_RS14555"
                     /old_locus_tag="H4J15_014555"
     CDS             14745..15698
                     /locus_tag="HI848_RS14555"
                     /old_locus_tag="H4J15_014555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222572.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNFL
                     KCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCS
                     VSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHL
                     EGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITF
                     DCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMEFNQVNQGF
                     ISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
                     /name="IS30 family transposase"
     gene            15676..17151
                     /locus_tag="HI848_RS14560"
                     /old_locus_tag="H4J15_014560"
     CDS             15676..17151
                     /locus_tag="HI848_RS14560"
                     /old_locus_tag="H4J15_014560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="WP_000443455.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIINK
                     DGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGS
                     KRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKN
                     DENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLEN
                     LKDYKVLRYRDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKK
                     DKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFN
                     KERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMII
                     NKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKK
                     SFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
                     /name="RNA-directed DNA polymerase"
     gene            17304..18383
                     /locus_tag="HI848_RS14565"
                     /old_locus_tag="H4J15_014565"
     CDS             17304..18383
                     /locus_tag="HI848_RS14565"
                     /old_locus_tag="H4J15_014565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_002326691.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCVP
                     QTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADTDA
                     FIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDLLEK
                     LSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVLLTLV
                     ERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLHDALQD
                     VANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDYPRKILG
                     YATPHDTFVQAFRQERLTA"
                     /name="IS30 family transposase"
     gene            18878..>20104
                     /locus_tag="HI848_RS14570"
                     /old_locus_tag="H4J15_014570"
                     /pseudo=""
     CDS             18878..>20104
                     /locus_tag="HI848_RS14570"
                     /old_locus_tag="H4J15_014570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588999.1"
                     /GO_function="GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA]; GO:0009007 -
                     site-specific DNA-methyltransferase (adenine-specific)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA];
                     GO:0009307 - DNA restriction-modification system [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /name="type I restriction-modification system subunit M"
     gene            complement(20162..20842)
                     /locus_tag="HI848_RS14575"
                     /old_locus_tag="H4J15_014575"
     CDS             complement(20162..20842)
                     /locus_tag="HI848_RS14575"
                     /old_locus_tag="H4J15_014575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <20918..21106
                     /locus_tag="HI848_RS14580"
                     /old_locus_tag="H4J15_014580"
                     /pseudo=""
     CDS             <20918..21106
                     /locus_tag="HI848_RS14580"
                     /old_locus_tag="H4J15_014580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            21099..21806
                     /locus_tag="HI848_RS14585"
                     /old_locus_tag="H4J15_014585"
     CDS             21099..21806
                     /locus_tag="HI848_RS14585"
                     /old_locus_tag="H4J15_014585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002353391.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFIQ
                     RVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSEL
                     VENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLTCGT
                     ESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGK"
                     /name="hypothetical protein"
     gene            21971..22171
                     /locus_tag="HI848_RS15225"
     CDS             21971..22171
                     /locus_tag="HI848_RS15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002330566.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRFV
                     LALVSLLFKMIAAIFFIGLFR"
                     /name="hypothetical protein"
     gene            22187..22366
                     /locus_tag="HI848_RS14590"
                     /old_locus_tag="H4J15_014590"
     CDS             22187..22366
                     /locus_tag="HI848_RS14590"
                     /old_locus_tag="H4J15_014590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305761.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLLI
                     AQEKAGFDIPALKK"
                     /name="hypothetical protein"
     gene            22707..22970
                     /locus_tag="HI848_RS14595"
                     /old_locus_tag="H4J15_014595"
     CDS             22707..22970
                     /locus_tag="HI848_RS14595"
                     /old_locus_tag="H4J15_014595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287514.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKR
                     LIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
                     /name="hypothetical protein"
     gene            22964..23314
                     /locus_tag="HI848_RS14600"
                     /old_locus_tag="H4J15_014600"
     CDS             22964..23314
                     /locus_tag="HI848_RS14600"
                     /old_locus_tag="H4J15_014600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_002321032.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVT
                     VAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEELL
                     MKVRTVFQKVSK"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(23338..23952)
                     /locus_tag="HI848_RS14605"
                     /old_locus_tag="H4J15_014605"
     CDS             complement(23338..23952)
                     /locus_tag="HI848_RS14605"
                     /old_locus_tag="H4J15_014605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_010729795.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRLV
                     FQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGNPL
                     LDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQRRL
                     VYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
                     /name="recombinase family protein"
     gene            24402..25727
                     /locus_tag="HI848_RS14610"
                     /old_locus_tag="H4J15_014610"
     CDS             24402..25727
                     /locus_tag="HI848_RS14610"
                     /old_locus_tag="H4J15_014610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_000969590.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVG
                     VGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMG
                     IMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
                     /name="Y-family DNA polymerase"
     gene            25720..26070
                     /locus_tag="HI848_RS14615"
                     /old_locus_tag="H4J15_014615"
     CDS             25720..26070
                     /locus_tag="HI848_RS14615"
                     /old_locus_tag="H4J15_014615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000568378.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTEE
                     IAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIRHI
                     EFYQDNKWSRLE"
                     /name="hypothetical protein"
     gene            26067..26246
                     /locus_tag="HI848_RS14620"
                     /old_locus_tag="H4J15_014620"
     CDS             26067..26246
                     /locus_tag="HI848_RS14620"
                     /old_locus_tag="H4J15_014620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001021554.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHNFRFYIFPLYI"
                     /name="hypothetical protein"
     gene            26382..26672
                     /locus_tag="HI848_RS14625"
                     /old_locus_tag="H4J15_014625"
     CDS             26382..26672
                     /locus_tag="HI848_RS14625"
                     /old_locus_tag="H4J15_014625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000026576.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITRQ
                     RRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
                     /name="hypothetical protein"
     gene            27040..27828
                     /locus_tag="HI848_RS14630"
                     /old_locus_tag="H4J15_014630"
     CDS             27040..27828
                     /locus_tag="HI848_RS14630"
                     /old_locus_tag="H4J15_014630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000101106.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQGN
                     ATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVGSE
                     HRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKLSET
                     IETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRTITVD
                     EAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
                     /name="AAA family ATPase"
     gene            27815..28141
                     /locus_tag="HI848_RS14635"
                     /old_locus_tag="H4J15_014635"
     CDS             27815..28141
                     /locus_tag="HI848_RS14635"
                     /old_locus_tag="H4J15_014635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000796719.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKDQ
                     GSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLAKK
                     QNKI"
                     /name="hypothetical protein"
     ARG             7151..8115
                     /name="vanH-A"
                     /gene="vanH-A"
                     /gene_name="D-lactate dehydrogenase VanH-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4275..5425
                     /name="vanS-A"
                     /gene="vanS-A"
                     /gene_name="vancomycin resistance histidine kinase VanS-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             10185..11092
                     /name="vanY-A"
                     /gene="vanY-A"
                     /gene_name="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             9149..9753
                     /name="vanX-A"
                     /gene="vanX-A"
                     /gene_name="D-Ala-D-Ala dipeptidase VanX-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7151..8118
                     /name="vanH gene in vanA cluster"
                     /gene="vanH gene in vanA cluster"
                     /gene_name="vanH; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3602..4296
                     /name="vanR gene in vanA cluster"
                     /gene="vanR gene in vanA cluster"
                     /gene_name="glycopeptide resistance gene cluster; vanR"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             10185..11095
                     /name="vanY gene in vanA cluster"
                     /gene="vanY gene in vanA cluster"
                     /gene_name="vanY; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             11249..11733
                     /name="vanZ gene in vanA cluster"
                     /gene="vanZ gene in vanA cluster"
                     /gene_name="vanZ; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4287..5428
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.74"
                     /coverage="98.96"
     ARG             9149..9756
                     /name="vanX gene in vanA cluster"
                     /gene="vanX gene in vanA cluster"
                     /gene_name="vanX; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.5"
                     /coverage="100.0"
     Typing          2..1041
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgatt ttaattatta caaatcaaaa gaaatttatc gtgaaaaata ttatcaaatg
       61 cctaaggtat tttttactaa cgaaaaatac atggatctat caaatgatgc aaaaattgct
      121 tatatgctac ttaaagatcg atttgattat tcagtcaaaa ataactgggt agattctgat
      181 gataatatat ttttcatatt cacagttgaa gaattaatga aattgttaca atgccgcgaa
      241 ggtaaagtat ctaaaatcaa aaaggaatta gaaaaagcag gtcttctgaa acagaagaga
      301 ggaagagtaa ataaacgtga cggtaagatt gaatccatgc ctaatttatt gtacttaggc
      361 aagccagaag taacgaatca agacgtcttt aaaatcatgg aagaggaaga caatactgat
      421 accaccgtaa ttgcgaaaat cgcaaatacg gcaaaaccgc gagaaatcaa tgataccacc
      481 gtaattgcga aaatcgcaaa tacggcaaaa tccagtcata tcaaggactc aaccgtaatt
      541 gcgaaaatcg cagacaatct attctattct aattctatag acactaatag acacataata
      601 gacactgaaa ctgatcagtt acaaaatcaa gttttactag ataacttcgt cgacatcatg
      661 aaagaggaca gtataaatac atttgtacca gaaaatgtat taaacttgat aaaaacattt
      721 tctagtactt acgaagacgc tcaaaaaacc gtccaaacta ttcataatgc aaagaaaaaa
      781 gctgaagaat tagagcaaac agttgttgtt tatgaagaac tggaatctta tggaattgat
      841 gctgacaaag gattatatat gactttgctg aaagcttatc aaaaacaaaa aactgaaaaa
      901 gtaaacaatt tgcagaatct aattttcgtt tatgtaaaaa attggtttgt cgaaaaagct
      961 attgcggcaa agcttgcaaa cgaacaaaga gaaacattta acgaattacc aacagttagt
     1021 acggaaaatt ggctagagta gttgataaaa agcgatcact ataaaaatga tcaattttaa
     1081 attagaactt ttttatgatt ttaattttat tacaaatgtt acaaataaat agctattatt
     1141 ccggagtttt tagattaaaa gtttatgaaa acaatcacaa acgtaggttt taaagtcata
     1201 atcgttagat attttagtta tttttcattc aaaaaaatag ggaactagtg tttgcatatg
     1261 tgcttttttg agttataata attcatgaag agaggtaatt cacttatttc cccaagctga
     1321 aaagcctgat attacttctt ttcaggcagt gctgctaata ttcaattaca cttttttgta
     1381 tatttttagt tgttttaaat ttaattgtct gatatgcttg tgatgaattg attttcgttc
     1441 tgcacttttg gacgataaat tgtgataatt gaaaatacaa aaaaaagaaa ggcccctgta
     1501 aggctcggga gactggtaat ctccgtcagt gtcctacagg aaagcattaa gatgacgcct
     1561 taatgcgtgc ccttcagtgt tacggtatta cccgcaacgg acggttctgt tgcaaagttt
     1621 taaataaaga ataaaatccc ttacggtatc tatgatttaa gctgggattc ccaataatac
     1681 cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg ttttcttata
     1741 taatcctcga atggcttcca tgcctttaat cgtggtagag gcagtgcgta aacttcgata
     1801 gaatttattg cgtctcttta ctggacgatg gtcttgttca atcaaattat tcaggtattt
     1861 aatggtacga tgttctgtcc cttgataaaa gccgtattct tttagtttct taaaggcact
     1921 tgtaatagag ggggctttat ctgtgactac aaccttcggt tcatcaaact gcttcactaa
     1981 ccgcttaaga aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa
     2041 ggttaaacca tctgcatcga tggctcgata caaataatgc cattttcctt taattttgat
     2101 gtacgtttca tccattttcc atgaataaaa ggatttttta tttttctttt tccaaatttg
     2161 gttctgttgc aaagttttaa ataaagaata aaatccctta cggtatctat gatttaagct
     2221 gggattccca ataatacctt gatttcagta cagaccgaaa acccgaagag agtgccttct
     2281 tttcgggttt tcttatataa tcctcgaatg gcttccatgc ctttaatcgt ggtagaggca
     2341 gtgcgtaaac ttcgatagaa tttattgcgt ctctttactg gacgatggtc ttgttcaatc
     2401 aaattattca ggtatttaat ggtacgatgt tctgtccctt gataaaagcc gtattctttt
     2461 agtttcttaa aggcacttgt aatagagggg gctttatctg tgactacaac cttcggttca
     2521 tcaaactgct tcactaaccg cttaagaaaa gcataggctg cttgtgtgtc ccgtttttta
     2581 cgtaaccaaa tatccaaggt taaaccatct gcatcgatgg ctcgatacaa ataatgccat
     2641 tttcctttaa ttttgatgta cgtttcatcc attttccatg aataaaagga ttttttattt
     2701 ttctttttcc aaatttgata gagtagtttg ccatattctt gcacccaacg ataaatcgtc
     2761 gtatgagaaa cgttaatgcc acgatcatat aagatttctt gaacttcacg atagctaagg
     2821 ttataacgaa gatagtagcc cacggctaca ataatcacat cctgctgaaa ttgctttcct
     2881 ttaaaatgat tcatcgtcat tcctcctgct atctttttct attattctac cttatttgat
     2941 agtagattta aaactttgca acagaaccta caggaagatg acatcattta tgttacagac
     3001 ttaactcgaa tcactcgtag tacacaagat ctatttgaat taatcgataa catacgagat
     3061 aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga agataatcca
     3121 tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg agatcttatt
     3181 cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt taaaggtcga
     3241 ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct atataaagaa
     3301 ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc ttcattatac
     3361 aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg caatattttt
     3421 aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga aaagcccttt
     3481 gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata
     3541 aattcggccc tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact
     3601 atgagcgata aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta
     3661 tacttaaaaa acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa
     3721 tgtatagaca agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc
     3781 ggccttacta tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc
     3841 gggaaagata cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata
     3901 acgaagccct ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac
     3961 aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc
     4021 attaatgtta acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc
     4081 gagttttcaa tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg
     4141 ctatttcatg agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg
     4201 catatccggc atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa
     4261 acggtatggg gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga
     4321 acgaaaactt tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta
     4381 tattcgttca atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa
     4441 atatgactta aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat
     4501 agatatcttt atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct
     4561 ttcaaaattc gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa
     4621 cgaagataaa caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac
     4681 attaaaacgg actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa
     4741 tgacgttgtt atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta
     4801 tttgagcctg cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca
     4861 tatcacgttg gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac
     4921 acggtataac ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct
     4981 ggtgcagatg accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat
     5041 tcacgccccc gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa
     5101 caacattttg aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc
     5161 gggcctctcc ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga
     5221 taagctagct gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac
     5281 gggtggcgcg ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca
     5341 gatttacgcg gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc
     5401 agacttggtt gataaaagga ggtcctaaga gatgtatata attttggctc tttgtcaaat
     5461 gatgttggta aagagaaatc ttagaataat tggagatcaa gtggatcagt tcctcttgat
     5521 cttttttagt tgaaaatcaa tgcgttgcca tgacgaatca tgatgcgcaa tttgaaatta
     5581 taaaagctac gaaagccata tgcatttcgt tttaacactt tgatatggtt gttcagacat
     5641 tcaagtgggc catttgaata actttgttgt aaagcatttt tgatttgtgg cagaaatttc
     5701 tggaacgttc tcaaggtcgt ctgatagcct tcgggaagca tgatattatt ggtaagtagt
     5761 tcttcaaacg atacgtagtc tcgttccttt acagcgtaaa gaaagtcttg atataactga
     5821 tagcctacac gcaaagcagg gctccctttc agtaagcgat ccacaatatc ggtctctgtt
     5881 aaatgagctt tgaagccagg tcgccaaagg cgtcgagtat aatcaagctt gcgttcatct
     5941 ttttgaagaa gtttccagta tctttttaat tgcttaccta attgatagtg tttttgttct
     6001 ttgcttttta atagttggtt cgtttctttg attcgatgat tgcgaaaggt acgaccaata
     6061 tgttggacga tgtgaaaccg gtcaataatc agctgtgctt ttggaaacag ctttttaacg
     6121 agcgatacat aaggagcgta catatcaatc acgatccatt cgaccgcttc acgaatttct
     6181 cgtgaaaaat gactaaaata acgttttaaa aaaggcagtt gtcggttctc gacaatgtcc
     6241 aataagcgct gtgtttgtcc atccatcatg atgaaactca tagcacctga aacaccccga
     6301 actgatttga attcatcaaa acaaagtacc ttaggtaacg gtgtccgaaa aggattcaac
     6361 ggacgataaa atcgcttcat aatacggtaa acagaagaga ccgatagatt tttctgagcg
     6421 gcgatctctg tcattgaggt attttttttc agacgagtca cgacagacca gcggactttc
     6481 tcactgatga aacaatgttt tttcgcaaca gaagtatctg cgacaaaggt ccgctgacaa
     6541 gctttgcatt tgaagcgttg ctttttcaat tcgaggtagg tacggtattc agaaacatcg
     6601 ttcattaaca accggacggt tgttgtaccc caacgcacaa tatctgaagc aatctggtgt
     6661 ttacagtgga tgcattcttg ggctgtataa atcaagtttc cacgataaat atgacagatt
     6721 tgccctttgt atttttcttt tgtgagacaa ttttcgttaa aagtaactga taagtctaga
     6781 atatctaatg tttctttgat aaggtgagtg taagacatct tgattcctcc ttttgggttg
     6841 gttcgccaaa ttaattctaa aggatttcaa ggtgtctttt ctatatgttt tggaaaataa
     6901 aaaagatgct ggtgtcatcc accaacatca aaaagtatag agccataatt ttttaggaaa
     6961 atctcaaggt tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct
     7021 cgttcttaca cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag
     7081 atttgacaag attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg
     7141 gtcttaatta tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat
     7201 gcattccatg ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg
     7261 tcggaatcca acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca
     7321 gagatttccg cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc
     7381 cgaagcatcg gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc
     7441 gacaatgtgg cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg
     7501 gcagtacgca acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac
     7561 agcgaccgtg gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata
     7621 ggcaaagcgg ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc
     7681 agccgaagta tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc
     7741 gttacgcttc atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata
     7801 cagagaatga agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc
     7861 tatgagttgg ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg
     7921 gaaggagagg aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt
     7981 ttacttaaac ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc
     8041 gagcaagcgt tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg
     8101 agacaggagc atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca
     8161 tgacgtatcg gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga
     8221 gccgttatac attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc
     8281 ggaatgggaa aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg
     8341 attacttgtt aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc
     8401 tttgcatggc aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat
     8461 cccttttgta ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata
     8521 catcgttgcg aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga
     8581 taggccggtg gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc
     8641 atccttcggt gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc
     8701 aagacaatat gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg
     8761 tgcggtattg ggaaacagtg ccgcgttagc tgttggcgag gtggaccaaa tcaggctgca
     8821 gtacggaatc tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt
     8881 tataaccgtt cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa
     8941 aaaaatatat aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga
     9001 taacggccgc attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg
     9061 ttatccccgt atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat
     9121 cgtattagcg ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata
     9181 gtacacggtg ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg
     9241 gttgacggtt atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg
     9301 aaggcaaaag aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt
     9361 cctaatcgtg ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca
     9421 aaggaaagtt attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct
     9481 tcaaaatcaa gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg
     9541 ggtgagcttg taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg
     9601 gcaaatggaa tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa
     9661 aacagtgggt ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca
     9721 taccccaata gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa
     9781 ctatataagc taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt
     9841 tatatatagt agatagtatt gaagatgtaa ggcagagcga tattgcggtc attatctgcg
     9901 tgcgctgcgg caagatagcc tgataataag actgatcgca tagaggggtg gtatttcaca
     9961 ccgcccattg tcaacaggca gttcagcctc gttaaattca gcatgggtat cacttatgaa
    10021 aattcatcta cattggtgat aatagtaaat ccagtagggc gaaataattg actgtaattt
    10081 acggggcaaa acggcacaat ctcaaacgag attgtgccgt ttaaggggaa gattctagaa
    10141 atatttcata cttccaacta tatagttaag gaggagactg aaaatgaaga agttgttttt
    10201 tttattgtta ttgttattct taatatactt aggttatgac tacgttaatg aagcactgtt
    10261 ttctcaggaa aaagtcgaat ttcaaaatta tgatcaaaat cccaaagaac atttagaaaa
    10321 tagtgggact tctgaaaata cccaagagaa aacaattaca gaagaacagg tttatcaagg
    10381 aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa gaaagtgtga agtcagatat
    10441 cgtgaattta tctaaacatg acgaattaat aaatggatac gggttgcttg atagtaatat
    10501 ttatatgtca aaagaaatag cacaaaaatt ttcagagatg gtcaatgatg ctgtaaaggg
    10561 tggcgttagt cattttatta ttaatagtgg ctatcgagac tttgatgagc aaagtgtgct
    10621 ttaccaagaa atgggggctg agtatgcctt accagcaggt tatagtgagc ataattcagg
    10681 tttatcacta gatgtaggat caagcttgac gaaaatggaa cgagcccctg aaggaaagtg
    10741 gatagaagaa aatgcttgga aatacgggtt cattttacgt tatccagagg acaaaacaga
    10801 gttaacagga attcaatatg aactatggca tattcgctat gttggtttac cacatagtgc
    10861 gattatgaaa gaaaagaatt tcgttctcga ggaatatatg gattacctaa aagaagaaaa
    10921 aaccatttct gttagtgtaa atggggaaaa atatgagatc ttttattatc ctgttactaa
    10981 aaataccacc attcatgtgc cgactaatct tcgttatgag atatcaggaa acaatataga
    11041 cggtgtaatt gtgacagtgt ttcccggatc aacacatact aattcaagga ggtaaggatg
    11101 gcggaatgaa accaacgaaa ttaatgaaca gcattattgt actagcactt ttggggtaac
    11161 gttagctttt taatttaaaa cccacgttaa ctaggacatt gctatactaa tgatacaact
    11221 taaacaaaag aattagagga aattatattg ggaaaaatat tatctagagg attgctagct
    11281 ttatatttag tgacactaat ctggttagtg ttattcaaat tacaatacaa tattttatca
    11341 gtatttaatt atcatcaaag aagtcttaac ttgactccat ttactgctac tgggaatttc
    11401 agagagatga tagataatgt tataatcttt attccatttg gcttgctttt gaatgtcaat
    11461 tttaaagaaa tcggattttt acctaagttt gcttttgtac tggttttaag tcttactttt
    11521 gaaataattc aatttatctt cgctattgga gcgacagaca taacagatgt aattacaaat
    11581 actgttggag gctttcttgg actgaaatta tatggtttaa gcaataagca tatgaatcaa
    11641 aaaaaattag acagagttat tatttttgta ggtatacttt tgctcgtatt attgctcgtt
    11701 taccgtaccc atttaagaat aaattacgtg taagatgtct aaatcaagca atctgatctt
    11761 tcatacacat aaagatattg aatgaattgg attagatgga aaacgggatg tggggaaact
    11821 cgcccgtagg tgtgaagtga ggggaaaacc ggtgataaag taaaaagctt acctaacact
    11881 atagtaacaa agaaagccca attatcaatt ttagtgctga ggaattggtc tctttaataa
    11941 atttccttaa cgttgtaaat ccgcattttc ctgacggtac ccccaaacta agatttcata
    12001 gatagatttt ctgataactt taaaaaacac gaatagcgga aaggatgtct tcattcgcta
    12061 aagagtaata aactactttt ccatcttgac gtgattttgc cattccatgt ttttttaaaa
    12121 aacgtaaatg gtgtgacgtt gtagcgacac tagcagtaag tatttctgct aaatcacaga
    12181 cgcacatttc cttataggtt tctaaagcat agaaaatttt aattctagaa aaatcgctga
    12241 aacatttccc taacacaagt aagtttgaaa aatctttttg atccaattta ttttttattc
    12301 catttacttt ttctttatgt acgacagtca cttggcaaac ttttttcatt catctcacct
    12361 cacattcaaa cgattatttg aatgaagtat acctatttga tcactctttt gtcaatctac
    12421 cttaattcca atacactatt ataagcaatt tcatgtattg aagtaaactt acttgcaagt
    12481 gcgtttttga ttccaagtta aaaacatatt attattctac ataatttcct aatcaaataa
    12541 aactcctttg aaaattgttc taaaggagtt ttattatttt gtatttctac taaaaatagc
    12601 gtacaatgta attactaagt aattacgaaa tgtaaaataa tggaggaaat aatatgatta
    12661 caaaaacaag aaaacaagga aattctatta tgttaacggt ccctaaagaa ttcgatgtgc
    12721 ccaatggtgt tgaagtagaa gctaagcttg tagagaatgg tatcctttat gagtttgtag
    12781 agcctaaaaa agaatttttt gattttagtg aagatatttt ggctgatatt ctttcagaag
    12841 gttatagcaa acaagagatc ttaaaagaat ttagaaatag aaaaaatgcg ttaacttctg
    12901 catttaaatc tattgctgaa gatactgttg caaattctaa accgatgaca aaagaggagt
    12961 tggcagcaga aattggctta tgagattcta ccttctaaac acgtaataaa gtatctaaaa
    13021 aaattaaaag agaaaacatt aaaagaacaa tttttaacga taatttatga tgagatagca
    13081 gttcgtcctc attcgggaga acaaaaaacg ggtgatttat caggaatttg ggctattgga
    13141 ttcaagtatg caggtacgac atatcgcgtt gcatatgaaa ttaaagataa tgcagtgata
    13201 cctaacttac tatgtggaac gcatgaaaat ttttatgaac aattaaaaaa gattaggtaa
    13261 gaacccaatg tttcaacttg agaggataga cgacacttta aaagataatt tttaacagct
    13321 gagagttgta ataccctata attttaaacc actaacctat atatttatga aaaatagaaa
    13381 aaattaaaac gcattttaag cctctttcaa attaaaagct tcatccttat gcacaaagag
    13441 ggcttaaaat gcgttatttt atattgtttt ttagtaataa taaaaaaatc actctaattc
    13501 tcccataaat attatagatt cctaaagatt aaaatagatt attccctatt gtcttttaag
    13561 tagcggtaga gtgttgcctt gctaatacca gtcatttccg taatttcttt tacgctataa
    13621 acctgacttt tatacaattt tactgctcga tcaacatctt tctggttcac tcgtggacgg
    13681 ccacctttac gacctctcgc acgtgcactt ttcaaacctt ctttggtacg ttcaacaatt
    13741 aaatcacgtt cgaactgact aaatgcttgg aagacagtca tcattaagcg tccgtgaggc
    13801 gtgctggtat cgaagttttc tttcaagctc accagcttaa catggtgttc ttcaaaatag
    13861 gtaacaaggt caatcaaatc ctttgtacta cgacccaatc gagaaaaact ttctacaatt
    13921 accgtatctc ccggacgaat tttatctttt aaacgattta gttggggacg aaccgctttg
    13981 gttcccgtca ttttctcagt taggatttca ttacaatttt gttcattgag taagtctaat
    14041 tgacgattta gttcttgtga acgtgtactc actcgggcat aaccataaat gtatccactc
    14101 atgcttttct tcctttcgta ctttaattct ggcttgattg tatcataaaa gggagttata
    14161 gatacatagt taatgcaaca agttatgata caaaatatga cctcttttat ttaataaaag
    14221 aggtcatttc attttgtctc ataatcgatt gtttttgata cgagtgtgta tacgaaataa
    14281 acaaaaaaat cttaagtaaa tttctagata cctaatttaa tcctaactag tgtatcctaa
    14341 agttaataac gagtttatca caatgaaaat tataaaatgc tcccatatta gatataacat
    14401 gcaatatttg aattagaact acaaaattta ttcgttttat aaagtaaata atgttgagat
    14461 actaaggaat agaatattat aaggaattga gctgaatgta ttggataaaa aaagaataac
    14521 tttattagag atggactata aaaatgctcg taattttctt ctgaagtctg aaagctattt
    14581 gaatgctcag ctaccagaat atattagttt tgacgaaact ttaaaatctg ctaaaaaagt
    14641 tttaacttcg ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact
    14701 caaattgtaa tacccgctaa agcatacaag gagagtgaac ataaatgact tatacccatc
    14761 ttacaccaaa cgagcttgta atgatagaag catattttca tcaagaaact ccggttgcta
    14821 tcgttgcgaa gcagcttaaa cgtggacgcc aaacaattta caatgtctat aactttctca
    14881 aatgtggtgg aacagcactt gaatactttg aacaatacaa agaaaataag cgacgttgtg
    14941 ggagaaccga aatcattttt cctgctgagg aaaaagaata cattgaaaaa agatcaactg
    15001 aaggttggac cccagacgtc attattggcc gtgcagagcg aaccttctct tgttcagtaa
    15061 gtaccctcta tcgccggttt aagacgggag aattcaatgt tttacattta ccgatgcaag
    15121 gaaaacgaaa accaaatggt tataaggaaa aacgtggaaa acaggcattc aaaagaaata
    15181 tttctgaacg taaaaaagat tatgtcgttt tcgaagaaga atttggacat ttagagggtg
    15241 atacgattgt cggcatccac cataaaagtg ccgtcatcac actcgttgag cgactttcaa
    15301 aagccattat cgccttgaaa ccagaaggcc gtaaggcagt tgatattgaa aattcgatta
    15361 atgaatggct tcaatccgta cccaaaaatc ttttcaaatc aattaccttt gattgtggaa
    15421 aagaattctc taattggaaa agtattagta atacgaatga tattgatatt tatttcgcag
    15481 acccaggaac gccttcccaa cggggattaa atgaacattc aaacggactc ctccgaaaag
    15541 atggactacc aaaagaaatg gaattcaacc aagtcaatca aggattcatc tcatccgttg
    15601 cgtccaaaag aaaccatatc cctagaaaat cactaaatta ccaaacacca ttagaagttt
    15661 ttttgagtta cgtaaatgga aagttttgtc tcgcttaatt tgacaaataa tacttcaaaa
    15721 aaaaaccaac cgaaagatat aaaagatatt gtagatacac gaatgtataa agaacgtgat
    15781 gacttaaact atacgcttat tatcaataaa gatggaaact attcatggcg tcctctcgtt
    15841 ctactacatc ctattctata tgtagatttg gttaattgtc tcactaaaga agaccattgg
    15901 gaggagttaa aaaagaggtt tagtgaattt aaaaaagata aaaatattaa ctgttattct
    15961 atacctgttg aagcttccaa aggttcaaaa aggacagatc ttggtgaaac aatactaaac
    16021 tggtgggaat attttgaaca aaaaagctta gctcagaata ttaaatacca atattgcatg
    16081 tttactgaca tcgctgattg ttatccatca atttatactc actctataac atgggccatg
    16141 tatggtaaag aaaaagttaa aaatgatgaa aataagtgtt cgggctcact tggcgataaa
    16201 atagataaaa aaatatctgg aatgcaacac aatcaaacaa atggtatccc tcagggttcc
    16261 gttcttatgg attttgtagc agagatcgtt ctagggtatg ttgatttgag attatcgaaa
    16321 gaattaaaaa aattagaaaa tttaaaagat tacaaagtac tcagatatag agatgattat
    16381 agaatatttt caaataatcg taatgatttg gaacaaatta tgaaagtgtt atcagaagtc
    16441 ttatatgatt taaattttaa gttaaattct agtaaaacaa aattatcaga tgatattatc
    16501 atagattcta tcaaaaaaga taagctctat tgggaagaaa ttaaggcaag tataagaatg
    16561 aatgccacaa acttagatgg tgatagagaa attcgttata gtataaattt acaaaaacat
    16621 ttattggaaa taaaaaagtt agcagataag catcctaatt caggccaatt gaaaaaagca
    16681 ctttctgagt ttaataaaga acgcattatt aaaatcactt caaagccgaa cgatctttta
    16741 ccacttattg gtatcctggt agatatcatg ataaataacc cgggttcttt gccgcagtgt
    16801 gtcttaatta tagggcagtt attaaatttc gaaaatgaga gtaaggctag agaaatagtt
    16861 gaaatgatta taaacaaata tgctttaaag ccaaatactg aatttatgat gatatggttt
    16921 caaagattga ccaccatttt tggagaaatg tttgatagtg ttttatcatc cgaatttgta
    16981 tctaaaatca aagatcctgt ctctaatcag atgttttctg tagactggct aaatgaagat
    17041 gctaagaaat ctttttatga agggaccatt aaagacgaag agaaatatat taaaatgagt
    17101 aagaaaatat atattactga atttgatgat tttaatgatt atccttttta agtgaaaatt
    17161 tagaatgaat ataaaaaaca ttacaatcta attttttggc ggattttata attaacttag
    17221 ccatttggaa ataaaaaaac gccacgtgaa ttccatgtta tattgaagtt accacaactc
    17281 tcaatagaca aggatgaatt cacatgacgc aaaaccatga taacacagtt tcacgcaagg
    17341 gaaaacacct aacctactca gaacgttgcc aaatcgccat cctgaaaaag gaaaactatt
    17401 ccaaccgtca ggttgcggat gtcctgggct gcgtgccgca gaccataaac aacgaaatca
    17461 aacgcgggac cgttactcag cttaaccgcc agaatcagaa cgggaaggtc tacgactatt
    17521 atcatgacgt ctacgacgcc gatgccgggc aggcggccta cgagaagaac cgcttgaact
    17581 gcggcagacg gcccttatgg gccgacacgg acgccttcat tgaatgggcg gacgacatga
    17641 tgctgttgga aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa cacgaattgt
    17701 tcgacccggc cattatccct gggaccacca ccctctatca gtggatcgac cgtggcatca
    17761 tgaaaacgaa gaatatggac ctgctagaaa aactgtcccg caaaccgaaa tcgtcccgtc
    17821 atggcaaccg cccgaataaa cgggtgctag ggaaatccat tgaagagcgc ccggaggcgg
    17881 ttggtacccg gaaaaccttc ggccattggg aagtcgacac ggtcgtagga aacaagacca
    17941 agtcggatgc cgtgctgctg acgttggtgg aacggcagac acgcttcgag gtcattctga
    18001 agctggccgg gaaggatgcc gaatcggtca accaagcggt ccgacagctg agagaacggg
    18061 ctggtgcgta cttcggccag ctgttcaaga cggttacctc ggacaacggc tcggagttcg
    18121 cgggactgca cgatgccttg caggatgtgg caaacatcta cttcagccac ccctatgctt
    18181 cctgggagcg cgggacgagc gaaaaccaac acaagctgat tcgccgtttc atccccaagg
    18241 gcagcccaat cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt tggatgaacg
    18301 attacccgcg aaagattttg ggctatgcaa cgccgcatga cacgttcgtc caggcattta
    18361 ggcaggaacg gttgacggct tgacacgtgg gcccttgaca acgagcctcc ctcggcatgg
    18421 cttgtgtctg gaaagctggc cggggccagc cagtcgggag actaccatgc ctcagctcat
    18481 tgtcaagggc ccactcgatt agaaaaatta gacggttggt ggtaacgagc aaccgggtca
    18541 ccatgggtgg ctaacttaaa cttgaaattt acgaatctaa tttttttaaa aaaatgttat
    18601 atcggaagta atctcttgat ttctatacta taagataaga aaaccactat ttaacaaggt
    18661 tattcgaacc ttattttaag ttacttatta tttaatgtat cgaaaaaggt gaaataggtt
    18721 tgttataata agaattaagt aagacatgaa ttgtctaagc aaatgtagct tatccatttc
    18781 attaaaatac atatggtgaa aaaacaagtt caaatagtaa ctaatttttt aaaaaactga
    18841 tttataaaaa atacaacacg aatgaggaaa ataaccaatg acagaacaaa tcgaaaaaaa
    18901 ttcaaaaagc ttatatcagg cactgtggaa ttccgcagat atcctgcgtt caaaaatgga
    18961 cgctaatgaa tacaagagct atttactagg cttggtcttt tataaatact tatccgacaa
    19021 catgctgcgg tatgtctccg ttctattgga ggaagaaacg gacgacctaa ataaagcaca
    19081 agacctttac gttgaagcat ttaaagatgc agatataaag gatgatctct tgtatgaact
    19141 aaaagatgag ttttcatata ccatcgcacc cgcattgacc tttaccgcgc aagtggcagc
    19201 cattcatgat ggatcttttc aattagaaga cctggtacaa ggtttccgtg acattgaaca
    19261 atccagcgaa aactttgaga acttatttga agacattgat ctgtattcta aaaaactggg
    19321 gtcaacccca caaaaacaaa ataaaaccat cgcagatgtt atgaaagaat tagaagggtt
    19381 aaacatggcg gggcatgctg gcgatgtctt aggcgatgcc tatgagtact taattggcca
    19441 attcgcatct gaatccggta aaaaagccgg tgaattctat accccacaac cggtcgcaaa
    19501 attgatgaca caaattgttt tacaaggaaa agaagataaa aaaggctttt ctgtttacga
    19561 cgcgaccatg ggttcgggct cgttactttt gaacgctaaa aaatattccc atcaaccggg
    19621 aaccatttct tatttcggac aagagttgaa tacgtctacc tataatttgg cgcgtatgaa
    19681 catgattttg catggggttc ccattgccaa ccaacacttg cataatgcgg ataccttgga
    19741 tcaagactgg ccaacagaag agccaacgaa cttcgatggg gtgctcatga acccaccgta
    19801 ttctgcaaaa tggtcggctg ataaaggctt cttagatgac ccacgcttct ctgcctatgg
    19861 cgtattagcc cctaaatcaa aagcggactt tgctttctta ttacatggct actatcattt
    19921 gaaagatacg ggcgtcatgg ccattgtttt gccacatggg gtgctcttta gagggggcgc
    19981 agaagggaag atacgcaaag ccttgttaga aaatggtgcc attgacaccg tcattggctt
    20041 acctgctaac atcttcttta atacgtccat tccaacgacc gtaattatct tgaagaaaga
    20101 ccgtgcgggt tctgttgcaa agttttaaat aaagaataaa atcccttacg gtatctatga
    20161 tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac ccgaagagag
    20221 tgccttcttt tcgggttttc ttatataatc ctcgaatggc ttccatgcct ttaatcgtgg
    20281 tagaggcagt gcgtaaactt cgatagaatt tattgcgtct ctttactgga cgatggtctt
    20341 gttcaatcaa attattcagg tatttaatgg tacgatgttc tgtcccttga taaaagccgt
    20401 attcttttag tttcttaaag gcacttgtaa tagagggggc tttatctgtg actacaacct
    20461 tcggttcatc aaactgcttc actaaccgct taagaaaagc ataggctgct tgtgtgtccc
    20521 gttttttacg taaccaaata tccaaggtta aaccatctgc atcgatggct cgatacaaat
    20581 aatgccattt tcctttaatt ttgatgtacg tttcatccat tttccatgaa taaaaggatt
    20641 ttttattttt ctttttccaa atttgataga gtagtttgcc atattcttgc acccaacgat
    20701 aaatcgtcgt atgagaaacg ttaatgccac gatcatataa gatttcttga acttcacgat
    20761 agctaaggtt ataacgaaga tagtagccca cggctacaat aatcacatcc tgctgaaatt
    20821 gctttccttt aaaatgattc atcgtcattc ctcctgctat ctttttctat tattctacct
    20881 tatttgatag tagatttaaa actttgcaac agaacccttg gaagtaggtg tcaaaccaag
    20941 cactatcaaa ccttccgaat atcaagcctt agaactgact tatgcaaaat tcgcacagat
    21001 taagaaagct aaagcaatgg ctagcgactg gctagatact tttgaaagta tcgaaaaaaa
    21061 tggtgcattt tatacgattg aatcggagga gaaagataat gaataaggtg gtttatcagc
    21121 taacaacaga agatgtttcg caatttttaa caagcaaaga acacttgaat gaagtaatca
    21181 gacggattca aaaagacttt agttgtatgg aagaaatgaa gtactttatt caaagagtgg
    21241 aagaacagtt gtatatggaa agcgaaacaa gtcaaacgaa ggtcacagtc agccataatc
    21301 atttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacagag cttctacgac
    21361 tagaaacaga aaaatacatc atgaatgtgt acctagaaag gagtgagctg gtcgaaaact
    21421 ttgtcagcta cacgatttgt aggaaacact attactcaga atcagaatat tattcaattt
    21481 tcgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa aatgcgacgg
    21541 tagacgatta ttaccttgta tttactaggt ttttgacgtg tgggacggaa tctatcgtgt
    21601 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
    21661 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gacacctaca
    21721 cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac
    21781 gatttgaaag gaaaaaaggt aaataaccat ttataccgaa aagtgaggat gtgtgaaata
    21841 agaacaaaaa accttgaaat taagggaatg gttcttattt cgcaccaatc gctccaaccg
    21901 cccccgccgc ttaggtggtg cgaaataagc gggcaaagcc cgataaccta agagaaagaa
    21961 ggagatccat atgaataaaa tcattcctct attgttcgta ctgtttgttg gcgagatact
    22021 tatgcgtcat gctgcagctg tacatttaaa cttcgtagta ttcttaggaa tactggtatt
    22081 ttgtttgatc ttttggcgat ttgtattagc ccttgtgagt ctactattca agatgattgc
    22141 agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc caattgcaat
    22201 aggaaataaa cggttacccg tgacattaga tgaaaagaga caaaaagaat tgcagcaact
    22261 aaagcagaag tacggaaaaa gtgaatctaa gattatgtgt attgcgttag atctattgat
    22321 cgcgcaagaa aaagcaggat ttgatatacc agcactaaaa aagtgacgtc acttttcatt
    22381 tgaagaaaca aaagtgatag cacttaattg ttcctactat ttagaatatt ttctatgtcc
    22441 tttttattcc attttgtatc aataactatg aaatctttaa agtatccaga agagattatt
    22501 tttagatcaa tttttttctt acctgattct aaagattttg ttttcttaaa catattatga
    22561 tttcctttcg actattttca tataaatgtt aacatagttt atggattcat ggaatgttaa
    22621 tctaatttaa tagaaaagga taattaagta tatacgtttt ttcttatgta tgttatacta
    22681 tgtatataca gaaagaggtg tctactatgg aaatgttacc tataaaaaaa tggggaaata
    22741 gtaatggcct tagattgcca aagtatatta tggaatatct tggaattcat acagaagaca
    22801 aagtgaagat aatacaagaa gaagtaaacg gtcagaagcg gctaattatc gaagcggcta
    22861 atatagaaaa tgaattaaca attgaacagt tatttgaaaa ttacactgat gaaagaaatc
    22921 atgtgacgat tcaaaattta ggtgaagcag taggtaatga aaaatggtga gaaaacctag
    22981 acaaggcgat attctattat tgaatacagc acctcgatct ggacacgaac agacaggtaa
    23041 aagaccttat attgttttaa gtcatgatat tattgcggat tatagtaacg ttgttacagt
    23101 tgctccaatt tctagtacaa ctagaaacta tccgctatat gtaaatataa actcagaata
    23161 tgaaatgaag acaacaggaa aagtattatt agatcaacta acgacaattg attatgaagc
    23221 cagagagtgt atttttttgg aaaaagcgca tgatagttta gttgaggagt tattgatgaa
    23281 agttagaaca gtttttcaaa aagtaagcaa ataaaataga aacagcagca tgatcaacta
    23341 ttcgtgattg accatgctgt ctttttttat tctatatacg gtttgtctcg taacattgta
    23401 gtcttttgca atcttagcga tcgcaacacc tttttttaga tcctcaacga tgtttttata
    23461 aacaagacga cgttggggat cttttgcatt ggcactgtat agcttaggac gtcctttata
    23521 aacaccgttc gcttttgcaa tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg
    23581 ttcttgttca gcgatcatgg ccaaaatttg aatgatcaaa tctttaatga atttatccag
    23641 gagaggattg ccaattgctt cggccatgat cggtagactt gtgatgatca gctgcacatt
    23701 tttcttcttc aagtaattga ctgaagcgat gatctcgtcg taatttcttc caagtcgatc
    23761 aatagcttcc acaataaaaa tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac
    23821 aagtcgatga gtaatcgttg cgccagattg tttctcaatg aaaattctct ctgcaccgaa
    23881 ttttttcatt tcttcaatct gccgttgttc attttgatcg atggatgaga cacgtatgta
    23941 tgcaactctc aaaattttta cctactttct gcaactattt aaaaaacacc ctaatcatac
    24001 acgagaaaac cagatttttc aatgtcgcgg tcgtgtctag ttagggtaaa ccttatatag
    24061 acaaaaacta atgatggagg aaatagtatg agaatttttt gtaagtgtct actgataact
    24121 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    24181 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    24241 ttcaaattaa ttagtaaagt gatagaataa attgatcata caaatcaaaa gaagaagcag
    24301 ggtgatagca tacctatgct tcttttttta tgtatttagc tatttcttct ttacaatatc
    24361 gaacatatgt ttgtataatg tgaaagaatg gagtgttatt tatgatggat tattcaaaag
    24421 agccagtaaa tgattacttt ttaatcgata tgaaaagctt ttacgcaagt gtcgaatgta
    24481 tagaaagaaa tttagatcca ttaacaacag aacttgttgt tatgagtcga gctgataata
    24541 ctggttcagg attgatatta gcttcttctc ctgaagcaaa aaagcggtat ggtattacaa
    24601 atgtgagtag accacgtgac ttaccacaac catttcctaa aacactacac gttgttccac
    24661 cacgtatgaa cctatatatc aagcgaaata tgcaggtaaa taatattttc agaagatatg
    24721 tggctgatga agatctactg atttactcga tcgatgaatc aatcttaaaa gtgacccgat
    24781 cactgaatct ttttacgact gaaggaacac gaagtcaacg gagaaagaag ctggctcaaa
    24841 tgatccagga gaatattaaa gaagagctag gattgattgc tacagtaggt gtcggagata
    24901 atcccctttt ggctaaacta gccttagaca acgaagcaaa gcataatgaa ggatttgttg
    24961 cggaatggac gtacgaaaac gttcctgaaa aagtatggaa tattcctgaa atgacggact
    25021 tttggggaat tggctctcga atgaaaaaaa gactcaatca gatggggatt atgagcatta
    25081 gagatcttgc taactggaat ccttatacga ttaagaatag actaggtgtg attggcttac
    25141 agctgtactt ccatgctaat gggatcgatc gaacagatat agcaatccca ccagaaccga
    25201 caaaggaaaa atcatatgga aattcacaag tgttaccacg agattatact agaagaaatg
    25261 aaatcgagct ggtggtaaaa gaaatggcgg aacaagtagc gatacgcatt aggcagcata
    25321 actgtaaaac aggttgtgtc catttacaca ttggcacgtc gatcttagaa acgagaccag
    25381 ggttttcaca ccagatgaag atccctatca cagataatac aaaggaattg cagaattatt
    25441 gtcttttctt gtttgataag tattatgaag gacaagaagt aaggcatgtt ggaataacct
    25501 atagcaagct tgtgtacacc gattcgcttc agctggattt atttagtgat ccacaaaagc
    25561 aaattgacga agaaaattta gataaaatta ttgacaagat tcggcaaaaa tatggtttta
    25621 cgtcgattgt acatgcttct tcaatgttag aaggcgctag aagcattact agaagcacac
    25681 ttgttggtgg tcatgcgggc ggaaatgggg gaattaaaaa tgattgatta caatgatcgt
    25741 ggacgtttaa agtgggcagg gttttattta tctgatcata ctgaagaaat tgatgccgac
    25801 aatgaacaac gaaccagtca aaatttagca aaagaacaaa tgacgactga agagattgcg
    25861 gaagtattga acgctgcagt cataaaaaat agatcagtat ctattcaaaa agaagaacga
    25921 aacgctgaag gatactatcc agaagatatc attggcaaga tcttaggtag tgatgagctg
    25981 gggatctacg ttggaaaaga caaaattcac tacgacgaaa tacgtcatat cgaattttac
    26041 caggataata aatggagtcg attagaatga acattatcca gcaatatgag ctaaagtata
    26101 taacctttga tcagctatca gaagagattt ggggttatgg ccaacgtttg ataaatgagg
    26161 ttggtgttga gcgcttctct ttctatgtag aagctgcagc aggttatcat aattttaggt
    26221 tttatatctt cccgttatat atataaggta ttttacggat tctatagagt tatctctatg
    26281 aatccttttt tgtttttaca aataggagta gatactagaa taatgcgtgt gttaaactat
    26341 tattgcaaac gcaataataa aagaaagtag gcttacgcac catgtctatc aatacatttg
    26401 tttactcgca cccgatcaat gtttacatta tcaagaattt aggaatcact gtagaacaat
    26461 tttgtgagtt atatgcctac ccacaaggaa cggttgcaag ctggataacc cgacaacgta
    26521 gaataaaatc attacctgct tcattcgttt atgatttaag cctggcctct agtttaaaca
    26581 tgtctgatgt ttatgaaaag ctcctgtctt tagaaaaaga atatgattcc ttcaagatta
    26641 aacatgataa aaaaataaaa aaacatatat aatatcaatc acaattgtat aatattacga
    26701 tatcttaata tcatgatatc gtaatattgt ttttttctaa gaaaatgcac tataagaagg
    26761 gaattattag taacaatata gacagtttag taatgtttaa attattctag ttgctgctcg
    26821 caaaacatat ttcttatcat taataaaaaa ttttaaagta ataaaaagtg attattttaa
    26881 tttcgaataa catatgaata atctttatat ccgaaccgat atatatcttt aaaacaaaat
    26941 aatcacacaa tattacaata taatgcatat acgatattgc aatatcacaa tattatgata
    27001 taattatatt ataatataag atgaatgggg gatattttaa tggcaagaat attgtcagta
    27061 gccaatcaaa aaggtggagt ttctaaaact acgactagcg ttaatttagc agcatgtttg
    27121 aaatttgttt acggtaaaaa tgtactttta gtggacattg atcctcaggg aaatgctaca
    27181 gataatgtag gtttcgatat agatggaaca aaccaaccta caatttatga agtattaaaa
    27241 ggggaggctg acattacaga tgctattctg gactataaag gaattgatgt tttgccagcc
    27301 gacatagccc tttcttctgc agagagggaa tttactcaag taggaagtga acacaggctt
    27361 aaaagagtac ttcaaccaat caaggacaac tatgattata ttataattga ttgtccacca
    27421 agtttaggaa tgcttacagt caacgcattt actgtttctg atgaaattat cattccagtt
    27481 gaagctgcgt atttctcatt aaaaggacta ataaagttaa gtgaaactat tgaaacggta
    27541 aaagaatata cgaacgacaa gcttgaagta cgaggagttc tatttactaa gtataataag
    27601 cgttataaca tttctaaaga aatgacgtct gcagcaaccg aaatttctag agtcattggt
    27661 gcaaaaattt tcaaaactta tattcgtagg acaataaccg ttgacgaagc gcaagctgct
    27721 ggatcagatc taatcaattt taataaaccc tcaacagctg aagatgacta taaagcattt
    27781 acagaagaat atttaaaaga agttggtgat tttgatggca gaagataatc gcagtcaagc
    27841 tgcagtaagt agattactag gaaataaaaa taaaaagaat tttaaatcaa aagaagtaga
    27901 agaaatcgaa ttaaccaaga aaaatataag taaagtcata aaagatcaag gaagtagtgt
    27961 aagaacagca gatagcattc gtataaatcc aattctaatg tcagcactca aatattggac
    28021 gactattgct gaatcagaca aaagtaaacc agacgttata gaagatgcgc tattaaagtc
    28081 catacctgaa gaatacttga ttgaaggata taaactagca aagaaacaaa ataaaattta
    28141 atttacattc aatatcacga tatcataata tcgtgatatt ttttttataa aaatcagtag
    28201 tatcaaagct gataagtcgc ttatcttcat attgtaatat tacgatatcg tgatataata
    28261 ttattgtaat atcgtgatat agttactatg agcttaaacc ttgaaataga gcggtttgct
    28321 ttaatttatt ttttaaggta aactaaaaaa agataaaaaa agactactgc tttaacaaag
    28381 tttggcgact tatcagcagt agaaaaatat atagtggata gatatatttg tttaattcat
    28441 tgtaacatag ttttgaacaa aacgctatcc tacagaaaga ggattggct
//
