LOCUS       NZ_CP059719             3172 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain BUCT2930 plasmid p4buct2930, complete
            sequence.
ACCESSION   NZ_CP059719
VERSION     NZ_CP059719.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15595090
            Assembly: GCF_014117405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Liu,Z., Liu,Y., Liu,W., An,X., Zhao,Y., Wang,L., Tong,Y., Hu,Y.,
            Lin,W., Fan,H. and Song,L.
  TITLE     Whole genome sequencing of a strain of E. coli causing diarrhea in
            Paguma larvata
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Liu,Z., Liu,Y., Liu,W., An,X., Zhao,Y., Wang,L., Tong,Y., Hu,Y.,
            Lin,W., Fan,H. and Song,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2020) College of life science and technology,
            Beijing University of Chemical Technology, 98 Zizhuyuan Road,
            Haidian District, Beijing, Beijing 100048, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13; Canu v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: SPAdes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:40:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,897
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,578
            CDSs (with protein)               :: 4,578
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 70 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 36 of 200
            Pseudo Genes (multiple problems)  :: 36 of 200
            Pseudo Genes (short protein)      :: 4 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059719.1.
            Bacteria and source DNA available from Beijing University of
            Chemical Technology, 98 Zizhuyuan Road, Haidian District, Beijing.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BUCT2930"
                     /isolation_source="feces"
                     /host="Paguma larvata"
                     /db_xref="taxon:562"
                     /plasmid="p4buct2930"
                     /geo_loc_name="China:GuangXi"
                     /collection_date="2020-02"
     gene            complement(197..625)
                     /locus_tag="H1R14_RS00460"
                     /old_locus_tag="H1R14_00460"
     CDS             complement(197..625)
                     /locus_tag="H1R14_RS00460"
                     /old_locus_tag="H1R14_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(589..759)
                     /locus_tag="H1R14_RS00465"
                     /old_locus_tag="H1R14_00465"
     CDS             complement(589..759)
                     /locus_tag="H1R14_RS00465"
                     /old_locus_tag="H1R14_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            917..1345
                     /locus_tag="H1R14_RS00470"
                     /old_locus_tag="H1R14_00470"
     CDS             917..1345
                     /locus_tag="H1R14_RS00470"
                     /old_locus_tag="H1R14_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_138581343.1"
                     /translation="MTYSIKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1734..2057)
                     /gene="mobA"
                     /locus_tag="H1R14_RS00475"
                     /old_locus_tag="H1R14_00475"
     CDS             complement(1734..2057)
                     /gene="mobA"
                     /locus_tag="H1R14_RS00475"
                     /old_locus_tag="H1R14_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2341..2535
                     /locus_tag="H1R14_RS00480"
                     /old_locus_tag="H1R14_00480"
     CDS             2341..2535
                     /locus_tag="H1R14_RS00480"
                     /old_locus_tag="H1R14_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2067..2303)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          2571..3094
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.707"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(1738..2057)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.23e-62"
ORIGIN
        1 ggcctaactg aggctacact gaaaggacag tatttggtga catgtctcgc acaagactgt
       61 taccacggtt agagaatctg ccaggattaa ctaaccttcg aaaaaccacc tgccagggtg
      121 gttttttcgt ttttacaata gtgagattac gacgagactg aatgaatctc aagatttcat
      181 ttagcctgcc gctcgatcat tcaaagtttt gaggtagctt cattgggtca tggtatccaa
      241 gctctaaaag ctttatatgg ttcggtgtca gccgctggaa atgaataacc cagtcagaaa
      301 ccttgtattt cccatgaaac gtatctttaa aaaccggatc gcctaaatga gcatgccata
      361 atttgttagc tctagcatac ttctcaatag cttctttgtt ttcgtaatga tcaggcactt
      421 tccacgatgg cgatactttg ccagtccaag ctggatatcc ttttaaagga tcgccaaaga
      481 gattgtattt ttcaaaatgc tcaatgaaat catcgatttt atcgagtgtt tttgcagaat
      541 atctggatgt gtaataaagg ttgaaggtct cagagaatga gacaatattc aattgtggat
      601 tttgcgcgcc tgctccgctg cccatgctct tctctgctcc cgacttagac cagatggcat
      661 tgtaacagac ttctcattcg ctctggaaat caacagagac aaggatacgc caaaaacctc
      721 aatatcttta acaagatcat gacgttgttc ggttttcata tcgctaactc cgtatctaat
      781 tcaggcagga aaaaatcttt tcctcgtttt cagagtagtc ttttttggtt gcacaataat
      841 ggcgccagtg tcaccctatt catatgaata taccatataa tatatacttt aaatattttg
      901 gggcttagga aaatcaatga cttactccat taagaataaa tggaaagctg gcggtttgtc
      961 tcttggctgg acagcagtgc ctaccgtatt gttttttata caaaacgaga aaaagctaac
     1021 ttcagtggcc tttaacacac tacttaacct gatcgttcac tggtggagtt tgcaagagtg
     1081 gccgcatccg tcaatggaaa gtctagccat acgtatgggg gtttctgtac ggactgttca
     1141 gagagctatc aatgatcttg aaaaagctaa tctcttggac aagaaaccaa catcgaagag
     1201 tgatcgccga tatggtggtc gcaatattta tgatctaaca aagctagttg actacctaga
     1261 tacaatgggt ccatcagtag ctgaacaagt taagaaacca cgccataaaa aaccggtata
     1321 taccgtaaga aaaacgacag cttaaaaaag cacaatggaa ctgagcgcga gcgatgcacg
     1381 atagcaaaac gatatattac ttattgcatt ggtcttggta gccctgcttc tggctaaacc
     1441 actgtcagga gtaattggct ttgcctgtaa tgggttcatc gcttcacagt taaagttatc
     1501 gcatagctca tatatttacc ataatccctt aattgtacgc accactgaaa cgcgctgaac
     1561 gcgattctga cgcagacacc gcaaaaagag atgttttacc ccggaaaaac aggaaggatg
     1621 cgtcaggatc gttttcagct cgctgcatag ctatgcatga aagcgaatgg tgatcacttt
     1681 gggagcttac ggtgttcata ccgtcagttt tcgacagttt tctctccggg aagctaatct
     1741 gccataagcc tggataacag ggcacggtga tactccgtaa tagcgatcag cacctccgca
     1801 tactccctgt ccccgacacg cttgccgtcg ataaagagtt ttttctgtaa cgcccccagt
     1861 ctcataactt ctttcagtac ccgatcatca gtcagtgagt taactttctt accgagagct
     1921 gccgcccgta aaaaaccgga aacggtcttg ccgcattcag cagccctttt tctgatttcc
     1981 tggtcttctt ctgccgtcag acgaacaggg cgactgatag cccgcctgcg cgtattactt
     2041 ccacttcgtt ttgtcataaa acatggctcc gtatctgaca tgggtgtcgg ggcaaagccc
     2101 tgaccagggc aattgtaata gcgtgcatgt atgcgcggta taacaattgc acatcctgtc
     2161 ctgtcagcaa gctcgaatta ccgacaaagg cacggcagtt ttttggcagt gacagtgttg
     2221 tctgacagaa atgcccgtca aaaagccaag cggcagaata gcggcagcaa aacagtcgaa
     2281 aagtggtacg cgatataatc acacaacgca gatgacagac agaacggaga aaacgaaaag
     2341 atgaataagc agcagcaagc cgtactcaac atggcgggat tcataaaaag tcagagcctg
     2401 acgctgctcg aaaaactgga cgcactcaat gccgacgaac aggccgccat gtgtgagaaa
     2461 ctgcacgaac tcgcagaaga actccagaac agcatacaga cacgctttga agcggaaaat
     2521 cttaccggga tataacgcat ccggacagga tgcagaacgg catagaggag cgtaaggaga
     2581 aaatgtcaca taaggcgctc ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     2641 cgactgcggc gagcggtgtc agctcactca aaggcggtaa tacggttatc cacagaatcg
     2701 ggggataaaa ccggaaagaa catgtgagca aaagacaaag acccagaaga agggcgcgcg
     2761 ggaggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2821 tcagaggtgg cgaaacccga caggacttaa agataccagg cgtttccccc tggaagctcc
     2881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2941 tagggaagcg tggcgctttc tcatagctca cgctgttggt atctcggttc ggtgtaggtc
     3001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttaacgcc actggcaacg
     3121 gccactggta agcaggatgt gcgagagatg taacgagagt tcttgaagtg gc
//
