LOCUS       NZ_CP059717             2345 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain BUCT2930 plasmid p5buct2930, complete
            sequence.
ACCESSION   NZ_CP059717
VERSION     NZ_CP059717.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15595090
            Assembly: GCF_014117405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Liu,Z., Liu,Y., Liu,W., An,X., Zhao,Y., Wang,L., Tong,Y., Hu,Y.,
            Lin,W., Fan,H. and Song,L.
  TITLE     Whole genome sequencing of a strain of E. coli causing diarrhea in
            Paguma larvata
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Liu,Z., Liu,Y., Liu,W., An,X., Zhao,Y., Wang,L., Tong,Y., Hu,Y.,
            Lin,W., Fan,H. and Song,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2020) College of life science and technology,
            Beijing University of Chemical Technology, 98 Zizhuyuan Road,
            Haidian District, Beijing, Beijing 100048, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13; Canu v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: SPAdes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:40:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,897
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,578
            CDSs (with protein)               :: 4,578
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 70 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 36 of 200
            Pseudo Genes (multiple problems)  :: 36 of 200
            Pseudo Genes (short protein)      :: 4 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059717.1.
            Bacteria and source DNA available from Beijing University of
            Chemical Technology, 98 Zizhuyuan Road, Haidian District, Beijing.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BUCT2930"
                     /isolation_source="feces"
                     /host="Paguma larvata"
                     /db_xref="taxon:562"
                     /plasmid="p5buct2930"
                     /geo_loc_name="China:GuangXi"
                     /collection_date="2020-02"
     gene            789..1757
                     /gene="mobV"
                     /locus_tag="H1R14_RS00005"
                     /old_locus_tag="H1R14_00005"
     CDS             789..1757
                     /gene="mobV"
                     /locus_tag="H1R14_RS00005"
                     /old_locus_tag="H1R14_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_025766223.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            complement(1821..2147)
                     /locus_tag="H1R14_RS00010"
                     /old_locus_tag="H1R14_00010"
     CDS             complement(1821..2147)
                     /locus_tag="H1R14_RS00010"
                     /old_locus_tag="H1R14_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001070513.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQIDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          781..1754
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.462"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 cgcggcttcc gagacatccc cttctgtatc ttattactct ccgtactcat acatcggcaa
       61 aatcaggcgt ttttctgccc gttcggtgcg aacgaatttc tatgtacggg aagtggaaat
      121 tccgcttcct cgctcattcg ctcactacgt tcggtcatga gtctgcggcg aaaagtacga
      181 ttagtacttg aggtgagtac gattagtact tgaggtaagt acgattagta cttgaggtaa
      241 gtacgattag tacttgaggt gagtacgatt cttctctcta gcccttggtg tgcgcgggtt
      301 cccgtatcta tatctatata ttaaggggga aaataaaggc tgaaaaacgc catttttaaa
      361 catcgaaaaa cgagcttttt tgagtctgga gcgtacggat ttctatgtcc ggggagtgga
      421 aattccgctt cctcgaacgg acagactcgc ggcgatgccg ctccctaatc cgttcttgcg
      481 gcgagcggtt cctgctcttt caaaaacgat attcattgcc tgatttcgtc aagggcgagc
      541 gtagcgagtg ccatttaccc ttgacgactc accggataaa acaggcttgg cagtggtggg
      601 aggtgacgcg gagcgacgcc ttccacctgc cagaccgccg ttgattttga cctttggaag
      661 cgcagcttcc cgaacgattt tgcgaagcaa agtctagtga gtacactatc atcttgatag
      721 tggcccgccg gaggtatcgc aatgcgctcc cccgcctggc tgaatgaatg ccggaatcag
      781 gggggaacat ggcgaactac gcgataatgc gctgcaaaaa gctgacggga atgggaagcg
      841 tggccagcgc gttgcagcac tgctaccgag agcgtgagac gccgaacgct aacgctgaac
      901 ggacaccaga aaactactgc tctgtgtcca aatcaacgga tcaggcaatg gggcgtgtgc
      961 gggagctgct gccggaaaaa cgccgtaaag atgcggtact ggccgttgaa tacgttatga
     1021 cagcaagccc tgagtggtgg aaagaggcca caccacggca gcaggcggag tttttcgccc
     1081 gctctgagca atggctggag aagaaatacg ggaaggatcg tgttgtggcg gcggtggttc
     1141 accgggacga ggccaccccg cacctgtctg cgttcgtggt gccattgacg caggatggac
     1201 ggctgtccgc gaaagagttt attggcgggc ggtcaaagat gcgtgaagat cagagcacct
     1261 acgctgaatc agtaaaaaaa ctgggtcttg agcgcggcat cgagggaagc cgcgccacgc
     1321 atcagacagt tcagcactac tacgaatcga taaaccgtgg cacccgtagc caggtatcga
     1381 tctcgccgga ggcgctggag ccgcgtgtat tgcggaaggg cattttcacc aaagacgtgg
     1441 aagatcaggc agccatagcc aaacgtctaa gccacgcagt gaacgatggc tttgcaggaa
     1501 ccatcgctat ggcctctcag agcgcccaga acgcaaaaag agcgcgtgag ctacagaaga
     1561 ctatggatgc gcagcagaaa cgcctacaga gcgtcacaga gccttttaag gggctttcca
     1621 gagagcagat gaccgaaatc ctgatgatgg ctcagcggtt taagcagcaa aaccaggaga
     1681 aggagaaaca gcagcgtgtt gaacgtgaga aacagcgaca gatgcgatcg cgtggcatgg
     1741 gcggtatgga gcgttagttt tgatgcgatg caggataggg ctaacgccgc aaaatgacgc
     1801 catttgcggc attccagaga tcaggttctg ccgccctgta taacgctgag gccgtttttc
     1861 agtgcttttt tgtggttgct gacagtacct ttccagccga actggggatt taggcggtac
     1921 gttttgctcc gcccaatctt cggtccttcc agtatgatcc cgaaatcaat caggtttttt
     1981 accgcacgac tgacatttgt tttttgcata gtcagcgctt ctgcaatatc tatctgcgcg
     2041 acctggatgt agttctcgta atccagctct gcgagcaagg ccatcagtac acgcatctgg
     2101 tcatggttta gctcattggc aatggtaatt agcgcggctt gattcatagt aaagtgcctc
     2161 tggaatgatg acttttgttt gggacgaatc actgctacaa aaccacctaa ttcctcccct
     2221 gtactttggt ctacctgcgt gattctccgc atctaaaaat ctccaatttc aggaaaaata
     2281 gttatctctc aagataacta agttatctct agagataact attgtcaaaa actttccgct
     2341 tgtgg
//
