LOCUS       NZ_CP059716             2496 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain ZHH plasmid pZHH-5, complete
            sequence.
ACCESSION   NZ_CP059716
VERSION     NZ_CP059716.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15648651
            Assembly: GCF_014117285.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Xu,Q.
  TITLE     Occurrence of carbapenem-resistant Enterobacter cloacae coharboring
            blaNDM-1, blaIMP-4 and mcr-9 in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Xu,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2020) Department of Infectious Diseases, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, East
            Qingchun Road NO. 3, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-NOV-2019
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014117285.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:45:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,334
            CDSs (total)                      :: 5,217
            Genes (coding)                    :: 5,078
            CDSs (with protein)               :: 5,078
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 51 of 139
            Pseudo Genes (incomplete)         :: 104 of 139
            Pseudo Genes (internal stop)      :: 22 of 139
            Pseudo Genes (multiple problems)  :: 32 of 139
            Pseudo Genes (short protein)      :: 1 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059716.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="ZHH"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pZHH-5"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="30.3 N 120.2 E"
                     /collection_date="2019-08-08"
                     /collected_by="Xu Qian"
     gene            join(2493..2496,1..215)
                     /locus_tag="H2678_RS26510"
                     /old_locus_tag="H2678_26510"
     CDS             join(2493..2496,1..215)
                     /locus_tag="H2678_RS26510"
                     /old_locus_tag="H2678_26510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(513..698)
                     /locus_tag="H2678_RS26515"
                     /old_locus_tag="H2678_26515"
     CDS             complement(513..698)
                     /locus_tag="H2678_RS26515"
                     /old_locus_tag="H2678_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1064..1291)
                     /locus_tag="H2678_RS26520"
                     /old_locus_tag="H2678_26520"
     CDS             complement(1064..1291)
                     /locus_tag="H2678_RS26520"
                     /old_locus_tag="H2678_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          379..466
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          2214..2383
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          complement(1707..2289)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ttggtttttc ctccggagcc ctgatgactt ttaatgatga tgagcgacac ctgcttgtgt
       61 ccgttgtttc gggctggctc cggcgtgccg aaggggatgc cggagccatg atgctggatg
      121 cataccggca gattttgtct gaaaccgaac cggccgcaag ggcggtcatg ctcgaatttc
      181 ttgaatcagt tcgcattcat tacgtttcgt cctgagcatt tcctgaagca tttcctgaat
      241 ttttgtccat actcaatcca ggcggccgtc acggacggtg gtcgtcgttc catgatgctg
      301 ttatggaaga ttgagagtaa tctaacttga ttgagttccg ggcgtgtcgc ccggtttttt
      361 ttgctgtttc ccggtgtccg ctcccggaca ggatgtgcat ttgtaatacc gcgcacgcgg
      421 cggcatcaca aataccccgt tcagggctgc gccccgacac cccgccatcg cctcacggcc
      481 gcgcactgaa ttgcgcaccc gttcaccgat tttcacgcat ctttctcgcc tatctgtgcg
      541 ttcagcatgg cgtaaagcgc ttcagcctga tcgtgcagat gctcacagca gccggctgcc
      601 tcatccagat ccatctgttc gagccgttcg aggaggagga gtgtctgtga gcggataaat
      661 ttggccatgt tgagggcggc ggattcctgt ctggtcactg gtgattttcc ccgtctggtg
      721 atgatttttg cgtgaaaaga gcatgccgtc attctggtgc atagtcatgc atgccgttaa
      781 aatttaacag gcgcgtttct gacgggttcc ggggtggttt gttgtggttt ttgtcatggt
      841 tccgtcagaa acgcgctgag cgcgtctgag gcggtgcggg caacggatgt tatggtaaat
      901 tcttgggggc ttgattataa aataccgcac ttcctcttat gtcttgtgct ttttagacaa
      961 ccctgaaagc ttttagcttt ggtgatgcaa tttgctggga ttttggcgga gaaccacagg
     1021 taaagaaaaa ggccacatta gcggcctttt tcggagaata tgctcagtgc ggaatttcgg
     1081 agacttcttt aacgtcggtt ttattgatct gctgtttgac accattcgcg tctgtatagc
     1141 caagcaggcc tgagtctgat tctttaggtt tgccgtcgct gacaatagtg cgaccgtcat
     1201 tggtgtgtat ggcatagctt gtgcgcgtgc agccggtgag ggctgaaagc gcaactgccg
     1261 ctgcgaacac tgaaacaaaa atctttttca aagccagctc cttttatcca tatcagtttt
     1321 ggtcactaac catctaacta taggttattt tttatgtgaa aagaggcttg atggtgggct
     1381 gtatatcgag ttaggcgcga ggtgctatgg tcaaagtgtg gtgtcaagcg gggaggaggc
     1441 ttatatgaga tgtaatccag cagatacata taccaagatg atttaagtat tcctctttgt
     1501 gaggttggat gtctaattta taaaggatct tcttgagatc cttttttttc gcgcgtaatc
     1561 tcttgccctg taaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt taggtaatcc
     1621 tggcagatcc cctaaccgtg gtaacagtct tgtgcgagac atgtcaccaa atttgtcctt
     1681 tcagtgtagc ctcactaagg ccgccacttc aagaactctt gatacatctc tcgcacatcc
     1741 tgtttaccaa tggccgttgc caatggcgat tagtcgtgtc tttccgggtt ggactcaagt
     1801 tgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     1861 atcctggagc gaactgcctt cccggaactg agtgtcaggc gtggaatgag aaaccgcggc
     1921 cataacagcg gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
     1981 ccaccagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg
     2041 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg
     2101 tggccttatg cctttttcgc taagaacctt cctggcattc tccaggaaat ttctgctctg
     2161 tacgtaagcc cgtaccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2221 ggaagcggaa tatctcctgt atcacatatt ctgctgacgc accgatgccg cctttttctc
     2281 ctgccacatg aagcacttaa ctgacatccg catccgtgcc aacatattaa gccagtatac
     2341 actccgctag cgctgcgtga ctggtccggc gctgcgcccg gaacccgcct gtaccggtta
     2401 agcagccgtt ctggtctgac tgcgattttt ttcattcctg cccgctaccc tgtaaacctt
     2461 tcttctgcgt tgccgttaac aggaggtaag ccatgt
//
