LOCUS       NZ_CP059486             2020 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Enterobacter kobei strain IB2020 plasmid pIB2020_S, complete
            sequence.
ACCESSION   NZ_CP059486
VERSION     NZ_CP059486.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15594913
            Assembly: GCF_014041955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Marchetti,V.M., Bitar,I., Sarti,M., Fogato,E., Scaltriti,E.,
            Bracchi,C., Hrabak,J., Pongolini,S. and Migliavacca,R.
  TITLE     Genomic Characterization of VIM and MCR Co-Producers: The First Two
            Clinical Cases, in Italy
  JOURNAL   Diagnostics (Basel) 11 (1), 79 (2021)
   PUBMED   33418979
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 384.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/08/2024 02:36:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,328
            CDSs (total)                      :: 5,207
            Genes (coding)                    :: 5,042
            CDSs (with protein)               :: 5,042
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 45 of 165
            Pseudo Genes (incomplete)         :: 121 of 165
            Pseudo Genes (internal stop)      :: 21 of 165
            Pseudo Genes (multiple problems)  :: 18 of 165
            Pseudo Genes (short protein)      :: 1 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059486.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="IB2020"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="pIB2020_S"
                     /geo_loc_name="Italy: Modena"
                     /lat_lon="44.64 N 10.92 E"
                     /collection_date="2019-12-14"
                     /collected_by="Agostino-Estense-Baggiovara Hospital"
     gene            310..546
                     /locus_tag="H1R18_RS26515"
                     /old_locus_tag="H1R18_26475"
     CDS             310..546
                     /locus_tag="H1R18_RS26515"
                     /old_locus_tag="H1R18_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(799..1116)
                     /locus_tag="H1R18_RS26520"
                     /old_locus_tag="H1R18_26480"
     CDS             complement(799..1116)
                     /locus_tag="H1R18_RS26520"
                     /old_locus_tag="H1R18_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023224510.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEATGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     Typing          2..146
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.89"
                     /coverage="83.0"
                     /evalue="3.43e-60"
     Typing          complement(1524..2014)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.755"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 tcgcatattc tgctgacgcg ccggtgccgc cttttttctc ctgccacatg aagcacttca
       61 ctggtattcg catccgtgcc aacatagtaa gccagtatac actccgctag cgctgcgtga
      121 ctggttcagg gctgcgcccc gaaacccgct aaaaccactg acgcgcctgc ggcttgccca
      181 acgtctcacg tcaccggatt aaaatccgct cccgttcacc gtttctggct gtctttcagc
      241 accgccagcg cttcatcggt tctgctgtgt ctggcagact gtgcggcgtt attctgatgg
      301 gagaatgcta tgaacagact gacaaacctg acacctgcag agaaaaaatt tcttgatgat
      361 gctattgccg cagctgagag agcgtcaggc aaaaagctaa atcagccgaa ccggcatatc
      421 gtcctgaaca gagcgcgtgc gcagattgag tcgcagcgtt acgccgaccg ccagcgtgca
      481 ttacgtgagg atgagcggca gcagtccgag tttgcctggt cacgtccgcg cgcgccgcgc
      541 cgctgattgc gccttttggc ggtttagtgg tactgacact ccgaaattgc cccccattca
      601 ctcctgtgaa tagccatgca gacgcgcaga aatcatcagg ggcgtttttc aggggttggt
      661 gcggtgaaag gtgccggaaa cggctgtcag gccgcagaaa cgcgctgagg gcctctcagg
      721 cagtgcagac aacggatgtt atggtaaatc cagctgattg gcaggtacaa aaaaagcccc
      781 gcagggcttt tttgtacctt attgatctgc acactttgca gccttcaggg cttccagctg
      841 atttagccgg ttagctgctt ttttgcgggc ttcgcctttt gccatgccat ttcctatccc
      901 aaaatcaccc aggaaaccta atacggtgcg cccgtcaaat tcgccggttg cctcaatttc
      961 ttgttgtaca ctgcgcgttt tggcgatttc cagagctacc gctttgcaat ccattgcgga
     1021 agattctgga ccggtgacgt tcgcggcctg tggatattgc tttgttgcgc atcctgccat
     1081 gatcagcgct gccgatagga tgaatatttt tttcaaaatt atccttattt atttaggcat
     1141 agttaagaaa tacccatttt atcggcagga aatgagggtt ttttagttga attttgggag
     1201 gggcaaggtt ccatattggt cgaggattct ggagctgacc ggcaacggcc agcgcggcta
     1261 tgggccatgc ggtggttgag catagaaagg atcttcttga gttccttttt ttctacgcgt
     1321 aatctcttgc tctgaaaacg aaaaaaccac ccggggaggt ggtttttcga aggttagtaa
     1381 atcctggcag attttctaac cgtggtaact ggcttcagca gagcgcagat accaaatact
     1441 gtccttccag tgtagccgta gttaggccac cacttcaaga actctatagc accacctaaa
     1501 ttaactcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgctt tgtcgtgtct
     1561 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcagtcgg actgaacggg
     1621 gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaactga gtgtcaggcg
     1681 tggaatgaga aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     1741 aggagagcgc acgagggagc caccaggggg aaacgcctgg tatctttaag tcctgtcggg
     1801 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
     1861 tggaaaaacg gcttcgccgc ggccttatcg cttccccgtt aagtttcgtc ctggcatctt
     1921 ccaggaaatt tccgccccgc cagtaagccc gcaccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaat atatcctgta
//
