LOCUS       NZ_CP059485             6127 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Enterobacter kobei strain IB2020 plasmid pIB2020_N, complete
            sequence.
ACCESSION   NZ_CP059485
VERSION     NZ_CP059485.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15594913
            Assembly: GCF_014041955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Marchetti,V.M., Bitar,I., Sarti,M., Fogato,E., Scaltriti,E.,
            Bracchi,C., Hrabak,J., Pongolini,S. and Migliavacca,R.
  TITLE     Genomic Characterization of VIM and MCR Co-Producers: The First Two
            Clinical Cases, in Italy
  JOURNAL   Diagnostics (Basel) 11 (1), 79 (2021)
   PUBMED   33418979
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 384.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/08/2024 02:36:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,328
            CDSs (total)                      :: 5,207
            Genes (coding)                    :: 5,042
            CDSs (with protein)               :: 5,042
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 45 of 165
            Pseudo Genes (incomplete)         :: 121 of 165
            Pseudo Genes (internal stop)      :: 21 of 165
            Pseudo Genes (multiple problems)  :: 18 of 165
            Pseudo Genes (short protein)      :: 1 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059485.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="IB2020"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="pIB2020_N"
                     /geo_loc_name="Italy: Modena"
                     /lat_lon="44.64 N 10.92 E"
                     /collection_date="2019-12-14"
                     /collected_by="Agostino-Estense-Baggiovara Hospital"
     gene            complement(join(6113..6127,1..126))
                     /locus_tag="H1R18_RS26460"
     CDS             complement(join(6113..6127,1..126))
                     /locus_tag="H1R18_RS26460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182115642.1"
                     /translation="MLRLLFVSRTTVSPPFSSALTTFSRLRIIPCATIVSSESVMACAA
                     F"
                     /name="hypothetical protein"
     gene            174..659
                     /locus_tag="H1R18_RS26465"
     CDS             174..659
                     /locus_tag="H1R18_RS26465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139156959.1"
                     /translation="MSGILDLAKAFEETSKQQAQRTGESVKSEFVKLEQSISEALKSSG
                     KSISDAIHEQNVRQTTLLRRSVTIALIWILFCGVLLIGILSGILWFQGTLIASRLDEMN
                     SYSQQLTDLKAKSGAGVEILTDDTRKNKYYVVLPKSAKGVELYTSKNDKQVVTYTAK"
                     /name="MbeB family mobilization protein"
     gene            720..956
                     /locus_tag="H1R18_RS26470"
     CDS             720..956
                     /locus_tag="H1R18_RS26470"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_037427059.1"
                     /translation="MTETEKLLLSALERLQDDYETRQQAWQASYDSLQTMFTTTSAALM
                     RSDQACQHLSGQVESLRQQVMSLTEAVSRLTRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1060..1368)
                     /locus_tag="H1R18_RS26475"
                     /old_locus_tag="H1R18_26435"
     CDS             complement(1060..1368)
                     /locus_tag="H1R18_RS26475"
                     /old_locus_tag="H1R18_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_037427062.1"
                     /translation="MQFMVYRNNSNNRAYPYLLDVQSDIIDELNTRLVIPLFLLDEVNG
                     RPARRLTPVLNVDGEDFLVMTHEMASVRQSQLGEEVVSVREHRQAIKNALDFLLDGF"
                     /name="CcdB family protein"
     gene            complement(1368..1607)
                     /locus_tag="H1R18_RS26480"
                     /old_locus_tag="H1R18_26440"
     CDS             complement(1368..1607)
                     /locus_tag="H1R18_RS26480"
                     /old_locus_tag="H1R18_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_064179603.1"
                     /translation="MPVSAMRHKKTVSVTIEPALLHQAREAGINLSATLTTALKHQIRE
                     SEAERWKRANRKAIQELNRITDEHGLLSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            1889..2812
                     /locus_tag="H1R18_RS26485"
                     /old_locus_tag="H1R18_26445"
     CDS             1889..2812
                     /locus_tag="H1R18_RS26485"
                     /old_locus_tag="H1R18_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_098947601.1"
                     /translation="MSNQALDLFNERLPRKPYFTDELQFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRPGAAIDWSDRNAPAPTLTIMNPENGHAHLLYALKTAVRTAPDGKIK
                     PLKYAAAVERALCKKLDADTGYSGLICKNPNHDHWQLAVWQPELYTLDWLADSLDLSAA
                     NDKEILPDYGLGRNCTLFDKTRKWAYRAIRQGWPDYEQWLRACYERANAYNLQFAAPLD
                     ENEVNGIAKSIAKWTKSKFSEETFGAYVSRTHSSEIQSVRGKKSRGGGRPKGRISIASD
                     ASLKPWVNLNISRSKYYRLGMNKRFL"
                     /name="replication initiation protein"
     gene            2812..3285
                     /locus_tag="H1R18_RS26490"
                     /old_locus_tag="H1R18_26450"
     CDS             2812..3285
                     /locus_tag="H1R18_RS26490"
                     /old_locus_tag="H1R18_26450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104652423.1"
                     /translation="MRLSLVASVFTLYLISLSSSVNAENNIFDMPINIPGSKYIDAAHP
                     TMSEWGSHCDSARNDIKEKGAWGFCNGYLAAVIQYDFFKRQKKCPNFSVGDFVQFATEE
                     SIEYRNKNAKPSTSPSTGKVIPNAYVYENVPAPGQRPAFLVLESIVMEKCNIN"
                     /name="hypothetical protein"
     gene            3316..3789
                     /locus_tag="H1R18_RS26495"
                     /old_locus_tag="H1R18_26455"
     CDS             3316..3789
                     /locus_tag="H1R18_RS26495"
                     /old_locus_tag="H1R18_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_084797509.1"
                     /translation="MKQSTLELDYLGYNFFREFARYEYCLKVTGFRKGTNTRVEADWYK
                     YAGEFVSLIESPSKELNESISYLINNPPKKQVLKDGLLCWSNVLPDHRNKSELVFSLIC
                     RIRNNLFHGGKFNGEWFSPQRSKELIEHALIILRTCGKSHSTVLKAYNGQHDE"
                     /name="hypothetical protein"
     gene            complement(3800..4021)
                     /locus_tag="H1R18_RS26915"
     CDS             complement(3800..4021)
                     /locus_tag="H1R18_RS26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_336244387.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVSL
                     GRNDLLIRTFPSGLFLALAISASASASY"
                     /name="hypothetical protein"
     gene            complement(4003..4983)
                     /locus_tag="H1R18_RS26500"
                     /old_locus_tag="H1R18_26460"
     CDS             complement(4003..4983)
                     /locus_tag="H1R18_RS26500"
                     /old_locus_tag="H1R18_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_213014372.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSQ"
                     /name="IS5-like element IS5 family transposase"
     gene            5297..5632
                     /gene="mobC"
                     /locus_tag="H1R18_RS26505"
                     /old_locus_tag="H1R18_26465"
     CDS             5297..5632
                     /gene="mobC"
                     /locus_tag="H1R18_RS26505"
                     /old_locus_tag="H1R18_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_037427024.1"
                     /translation="MDKRTKEIKIRLTESEHERLLARCDRAHLAEWLRSLGLGEHVLRR
                     RRVPDVDPALLRQVTGIGNNLNQIARKLHGLDSLSAHQQVSLLAVLTSLDRQLSELLET
                     QRDRQDS"
                     /name="mobC"
     gene            complement(5646..6059)
                     /locus_tag="H1R18_RS26840"
     CDS             complement(5646..6059)
                     /locus_tag="H1R18_RS26840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_244637524.1"
                     /translation="MRVIEVQAVIGQSLPGVDPGTISPVIVGLQAFSALQFDVREHKIQ
                     LQPVLVPVLHPQTGVLVAVQTGQQCLLKLIHQTHFLIVREVLFPERQHPGGIGAGKLTT
                     INQLPDTLRLTAQYHRFIPITFFSQQVIHRATA"
                     /name="hypothetical protein"
ORIGIN
        1 ggccattacg gactcgctgg aaacgatggt ggcacagggt atgatccgca accgtgagaa
       61 tgtggtcagg gcgctggaaa acggcgggct gacggtggtg cgggaaacga aaagcagtct
      121 cagcattgcc gacccggacg gtggacgaaa tatccggtta aaaggggcgt tatatgagcg
      181 ggattttaga tttagcgaaa gccttcgaag agacctcgaa gcagcaggca cagcgtacag
      241 gggagagcgt gaagagcgag tttgtgaagc tcgagcaatc catcagcgag gccttgaaat
      301 caagcgggaa gagcatcagc gacgctatcc acgaacagaa cgtcaggcag acaaccctgc
      361 tgcggcgctc ggtcacgatc gcgctgatat ggatattgtt ttgtggtgtg ctgctgatcg
      421 gtattctgtc ggggatactg tggtttcagg ggacactcat cgcctcacgt ctggacgaga
      481 tgaacagcta cagccagcag cttaccgacc tgaaagcgaa atcgggggcg ggggtggaaa
      541 tactcacgga cgacacccgg aagaacaagt attacgtggt gctgccgaag agcgcgaagg
      601 gcgtcgagct gtacacctcg aaaaacgaca aacaggtcgt gacctacacc gcgaaatagc
      661 cccgtcagtt gccacagaag cgccatacgg cgtttttagc gataaggaca agcaaatcca
      721 tgaccgagac cgaaaaactg ctgctgagtg cgttagagcg cttacaggac gattacgaga
      781 cacgacagca ggcatggcag gccagttacg acagcttgca gaccatgttc accactactt
      841 ccgccgcgct gatgcgcagc gaccaggcgt gtcagcactt gagcgggcag gtggagagct
      901 tgcggcagca ggtcatgagc ttaaccgaag ccgtcagccg cttgacgcgc aggtagccag
      961 actggacagg gagctggcgc agaaacagga acgacagaaa acgcgctcat ttgcccccgg
     1021 acgcagccgg ggacacgatc acgggatgga aaggtaaact cagaaaccgt cgagcagaaa
     1081 atccagcgca ttcttgatgg cctgccggtg ctcccggacg ctcaccacct cttccccgag
     1141 ctgtgactgg cgaacgctgg ccatttcatg tgtcatgacc agaaaatcct caccgtccac
     1201 attcagcacc ggggtcaggc ggcgggcggg ccgtccattc acttcatcca gcagaaacag
     1261 cgggatcacc aggcgggtat tcagttcgtc gataatatcg ctctgtacat cgagcagata
     1321 gggataagcc cggttgttgc tgttattgcg gtaaaccatg aactgcatca gaacgtcctg
     1381 tgatcgtcag agagtaagcc gtgctcgtcc gtaatacggt tgagctcctg gattgccttg
     1441 cggttggcgc gtttccagcg ctccgcttct gactcgcgga tctgatgttt cagggcggtg
     1501 gtgagcgtgg cagacagatt aatcccggct tcgcgggcct gatgcaaaag cgcaggctcg
     1561 attgtcacac tgacggtttt tttatggcgc atcgctgata cgggcatgat gatctcctga
     1621 tttttacacg tgtatatagc acgtgttgcc agtgtacgcg ttttttaccc ggaaggtaat
     1681 ggtctgttgc ttcaggcaat aactgacggt gacaggtgac aaaatacggc ggggtgtgta
     1741 acgttaagcg gcagaaaaga cgacgccccc gtaatcatgc tcgacttggc ggaaggacat
     1801 gattaacgag ggcgcaggca caaaacctaa gaaggatact agcacatgaa ccgtgcgaaa
     1861 caacagcccg ccataagcgc aggcgagatt gagtaaccag gcactcgacc tttttaacga
     1921 gcggcttccc cgcaaaccct attttaccga cgaactccag ttcggagtac gcattgccgg
     1981 gaaagagcgc gccattcttg cgaaatacat ccagttcaac cagccacacg cgatgttctg
     2041 gctcggtttt gacgttgacc gccccggcgc ggcgatagac tggagcgacc ggaacgcacc
     2101 tgcgccaacg ctgaccatca tgaatccgga aaacggacat gcccacttac tgtatgcgct
     2161 gaaaacagcg gtgcgcacgg cacctgacgg aaaaattaag ccgctgaagt atgcggcagc
     2221 ggttgagcgt gcgctgtgta aaaaactgga cgctgacact ggctattcag ggttaatctg
     2281 caaaaatccg aaccatgacc actggcagct tgccgtgtgg caaccggaac tctacacgct
     2341 cgactggtta gcagactcac ttgacctgag tgcagcgaac gacaaagaaa tcctacccga
     2401 ctacggttta ggccgtaact gcaccctgtt cgataaaacc cgtaaatggg cttatcgcgc
     2461 cattcgtcag ggctggcctg attatgaaca gtggttaaga gcctgctacg aacgtgccaa
     2521 cgcttataac ctgcaatttg cagcgcctct ggatgaaaat gaggttaatg gtatagccaa
     2581 gagcattgct aaatggacta agtcaaaatt tagtgaggaa acatttggtg cctatgtatc
     2641 tcgtactcat tctagtgaga ttcaatcagt tagaggaaaa aaaagccgag gaggaggtcg
     2701 cccaaaggga cgaatatcta ttgcatctga tgcaagttta aaaccatggg taaatttaaa
     2761 tattagccgt agtaaatatt atagacttgg gatgaataag aggtttttat aatgagactt
     2821 tcacttgttg cgtctgtttt tactctgtat ttaatcagct tatcttcttc cgttaatgct
     2881 gaaaataata tttttgatat gcctattaat atccctggca gtaaatatat tgatgcagcg
     2941 caccctacca tgtcagaatg gggtagtcat tgtgatagtg cacgaaacga tataaaagaa
     3001 aaaggagctt ggggtttttg taatggctat ttagccgctg ttattcaata tgattttttc
     3061 aaaagacaga aaaaatgtcc taacttctct gtcggagact ttgttcagtt tgctactgaa
     3121 gaatcgatag agtacagaaa taagaatgca aaaccatcga cttcaccttc tactggtaaa
     3181 gttattccta atgcttatgt ttatgaaaat gtccctgccc cgggccagcg ccctgctttt
     3241 cttgtgttgg aatctattgt aatggaaaag tgtaatataa attaaattac atattttttc
     3301 atttatgagg tttttatgaa gcaatctaca ttagaactag attatttagg atataacttt
     3361 tttcgtgaat ttgctcgcta tgaatattgt cttaaggtta ccggatttcg taagggcaca
     3421 aatactagag tagaagctga ttggtataag tatgctggtg aattcgtttc attgattgaa
     3481 agtccaagta aagagttaaa tgagtcaata tcatacctga taaacaaccc accaaaaaaa
     3541 caagttttaa aagatggttt attatgttgg tctaatgtat tgcctgacca tagaaataag
     3601 tctgagcttg ttttttctct tatttgtaga attagaaata atcttttcca tgggggtaaa
     3661 tttaatggtg aatggtttag tcctcaacga agtaaagaac tcattgaaca tgcgctaatt
     3721 attcttcgga cttgcgggaa gagccattct acggtgctca aagcatataa tggccagcat
     3781 gatgaataaa tacaatcaac taatatgatg ctgatgctga tgctgatatc gctaaagcta
     3841 agaacaatcc actagggaag gtgcgaataa gcaggtcatt tcttcccaag ctgactcgct
     3901 gattaaaatt tcgcggatct gggccgattt ttttcccgca aacacatcga atcagcctat
     3961 ttaggctatt ttttccacca tttctggcgt tatttccggt ttttactgag atctctccca
     4021 ctgacgtatc atttggtccg cccgaaacag gttggccagc gtgaataaca tcgccagttg
     4081 gttatcgttt ttcagcaacc ccttgtatct ggctttcacg aagccgaact gtcgcttgat
     4141 gatgcgaaat gggtgctcca ccttggcccg gatgctggct ttcatgtatt cgatgttgat
     4201 ggccgttttg ttcttgcgtg gatgctgttt caaggttctt accttgccgg ggcgctcggc
     4261 gatcagccag tccacatcca cctcggccag ctcctcgcgc tgtggcgccc cttggtagcc
     4321 ggcatcggct gagacaaatt gctcctctcc atgcagcaga ttacccagct gattgaggtc
     4381 atgctcgttg gccgcggtgg tgactaggct gtgggtcagg ccactcttgg catcgacacc
     4441 aatgtgggcc ttcatgccaa agtgccactg attgcctttc ttggtctgat gcatctccgg
     4501 atcgcgttgc tgctctttgt tcttggtcga gctgggtgcc tcaatgatgg tggcatcgac
     4561 caaggtgcct tgagtcatca tgacgcctgc ttcggccagc cagcgattga tggtcttgaa
     4621 caattggcgg gccagttgat gctgctccag caggtggcgg aaattcatga tggtggtgcg
     4681 gtcaggcaag gcgctatcca gggataaccg ggcaaacaga cgcatggagg cgatttcgta
     4741 cagagcatct tccatcgcgc catcgctcag gttgtaccaa tgctgcatgc agtgaatgcg
     4801 tagcatggtt tccagcggat aaggtcgccg gccattaccc gccttggggt aaaacggctc
     4861 gatgacttcc accatgtttt gccatggcag aatctgctcc atgcgggaca agaaaatctc
     4921 ttttctggtc tgacggcgct tactgctgaa ttcactgtcg gcgaaggtaa gttgatgact
     4981 catgatgaac cctgttccat ggctccagat gacaaacatg atctcatatc agggacttgt
     5041 tcgcaccttc cctagatgtt ctctgtgaga ctaaaaacaa cctatatcag ataacagcct
     5101 tctccagaga aggctttttt tgcagccagc acagaatctg acatacttat caatcactat
     5161 aagccagtac acacagcgtc ccctctacga gggttccttc tgtgactggt tcagggggct
     5221 cgccgccccc cgaaacccct ggcatccact gcgaaaattc gcactctggg tgcgaaagtt
     5281 ttctcagcgg agtgtcatgg acaagcgaac gaaagagata aaaatccgcc tgactgagag
     5341 tgaacatgag cgactgctgg cacgttgtga tcgtgctcat ctcgctgaat ggttacgttc
     5401 gctcggtctg ggtgaacatg tattgcgtcg ccggcgtgtg cctgacgttg atccggcact
     5461 gcttcgtcag gtgaccggca tcggcaataa cctcaatcag atagcccgta agctgcatgg
     5521 tctggattcg ctttcggccc atcagcaggt ttctcttctg gcggtactga ccagccttga
     5581 tcgccagtta tctgagcttc tggagacaca gcgtgatcgt caagattcat agccgcggtg
     5641 cgggttcagg cagtggcccg gtggattacc tgctgggaaa agaacgtgat cgggataaag
     5701 cggtggtact gcgcggtgag cctgagcgtg tcaggcaact gattgatggt tgtgagtttg
     5761 cccgcgccta tacctccggg gtgttgtcgt tccgggaaca aaacctccct gacgatgaga
     5821 aagtgcgtct gatggatgag tttgagcaga cactgttgcc cggtctggac ggcgaccagt
     5881 acgcctgtct gtgggtggag caccgggaca aggacaggct ggagctgaat tttgtgttcc
     5941 cgaacatcga attgcagagc ggaaaacgcc tgcaacccta ctatgaccgg gctgatcgtc
     6001 ccagggtcaa cgcctggcag actctgacca atgaccgctt gaacctcgat gaccctcatg
     6061 atcctgcccg acgacgcacc ctgacaccgg gcaatgacct tcccggcgac cgtcagaagg
     6121 cggcgca
//
