LOCUS       NZ_CP059482            12808 bp    DNA     circular CON 08-MAR-2024
DEFINITION  Enterobacter kobei strain IB2020 plasmid pIB2020_ColE_MCR, complete
            sequence.
ACCESSION   NZ_CP059482
VERSION     NZ_CP059482.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15594913
            Assembly: GCF_014041955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Marchetti,V.M., Bitar,I., Sarti,M., Fogato,E., Scaltriti,E.,
            Bracchi,C., Hrabak,J., Pongolini,S. and Migliavacca,R.
  TITLE     Genomic Characterization of VIM and MCR Co-Producers: The First Two
            Clinical Cases, in Italy
  JOURNAL   Diagnostics (Basel) 11 (1), 79 (2021)
   PUBMED   33418979
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 384.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/08/2024 02:36:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,328
            CDSs (total)                      :: 5,207
            Genes (coding)                    :: 5,042
            CDSs (with protein)               :: 5,042
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 45 of 165
            Pseudo Genes (incomplete)         :: 121 of 165
            Pseudo Genes (internal stop)      :: 21 of 165
            Pseudo Genes (multiple problems)  :: 18 of 165
            Pseudo Genes (short protein)      :: 1 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059482.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="IB2020"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="pIB2020_ColE_MCR"
                     /geo_loc_name="Italy: Modena"
                     /lat_lon="44.64 N 10.92 E"
                     /collection_date="2019-12-14"
                     /collected_by="Agostino-Estense-Baggiovara Hospital"
     gene            complement(join(12382..12808,1..41))
                     /gene="traD"
                     /locus_tag="H1R18_RS25060"
                     /old_locus_tag="H1R18_25040"
     CDS             complement(join(12382..12808,1..41))
                     /gene="traD"
                     /locus_tag="H1R18_RS25060"
                     /old_locus_tag="H1R18_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_032235402.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            207..1304
                     /gene="mobA"
                     /locus_tag="H1R18_RS25065"
                     /old_locus_tag="H1R18_25045"
     CDS             207..1304
                     /gene="mobA"
                     /locus_tag="H1R18_RS25065"
                     /old_locus_tag="H1R18_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_040113349.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            1317..1901
                     /locus_tag="H1R18_RS25070"
                     /old_locus_tag="H1R18_25050"
     CDS             1317..1901
                     /locus_tag="H1R18_RS25070"
                     /old_locus_tag="H1R18_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040113348.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(2235..3257)
                     /locus_tag="H1R18_RS25075"
                     /old_locus_tag="H1R18_25055"
     CDS             complement(2235..3257)
                     /locus_tag="H1R18_RS25075"
                     /old_locus_tag="H1R18_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_000156887.1"
                     /translation="MTITTVGIDLAKNVFAVHGVDQNGRTVLVKPKVSRAALPELIASL
                     PPCVIGMEACSGAHYWARLFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICEAV
                     RRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSADALRH
                     MVSVQKSSLPLQVQQCVDDLMEHVDRIEANITEYDRILSHIAKTDHRSQRLMELKGVGP
                     TTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRTLLVQG
                     ARSVLIGAGKRTDSFSRWVCMLVERRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSVN
                     "
                     /name="IS110 family transposase"
     gene            3617..4639
                     /locus_tag="H1R18_RS25080"
                     /old_locus_tag="H1R18_25060"
     CDS             3617..4639
                     /locus_tag="H1R18_RS25080"
                     /old_locus_tag="H1R18_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_000156887.1"
                     /translation="MTITTVGIDLAKNVFAVHGVDQNGRTVLVKPKVSRAALPELIASL
                     PPCVIGMEACSGAHYWARLFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICEAV
                     RRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSADALRH
                     MVSVQKSSLPLQVQQCVDDLMEHVDRIEANITEYDRILSHIAKTDHRSQRLMELKGVGP
                     TTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRTLLVQG
                     ARSVLIGAGKRTDSFSRWVCMLVERRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSVN
                     "
                     /name="IS110 family transposase"
     gene            complement(<4933..5718)
                     /locus_tag="H1R18_RS25085"
                     /old_locus_tag="H1R18_25065"
                     /pseudo=""
     CDS             complement(<4933..5718)
                     /locus_tag="H1R18_RS25085"
                     /old_locus_tag="H1R18_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            complement(6015..>6332)
                     /locus_tag="H1R18_RS25090"
                     /old_locus_tag="H1R18_25070"
                     /pseudo=""
     CDS             complement(6015..>6332)
                     /locus_tag="H1R18_RS25090"
                     /old_locus_tag="H1R18_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016707037.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(6501..7205)
                     /locus_tag="H1R18_RS25095"
                     /old_locus_tag="H1R18_25075"
     CDS             complement(6501..7205)
                     /locus_tag="H1R18_RS25095"
                     /old_locus_tag="H1R18_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <7267..7437
                     /locus_tag="H1R18_RS26780"
                     /pseudo=""
     CDS             <7267..7437
                     /locus_tag="H1R18_RS26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            7611..9236
                     /locus_tag="H1R18_RS25100"
                     /old_locus_tag="H1R18_25080"
     CDS             7611..9236
                     /locus_tag="H1R18_RS25100"
                     /old_locus_tag="H1R18_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-4.3"
                     /protein_id="WP_011638903.1"
                     /translation="MISRFKTLSVNQFTFITALFYVAIFNLPLFGIVRKGIEKQPEVDP
                     LFIASMPLFLTFALSFLFSIFTVKYLLKPFFIVLTLLSSSVFFAAYQYNVVFDYGMIEN
                     TFQTHPAEALMYVNLASITNLLLTGLLPSYLIYKADIHYQPFFKELLHKLAFMLLMFVG
                     IGIVAFFYYQDYAAFGRNNSELRRYIVPTYFVSSASKYLNEHYLQTPMEYQQLGLDAKN
                     ASRNPNTKPNLLVFVVGETARSMSYQYYGYNKPTNAHTQNQGLIAFNDTSSCGTATAVS
                     LPCMFSRMGRADYDPRRANAQDTVIDVLSHSGIKVQWFDNDSGCKGVCDQVENLTIDLK
                     SDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIGSHGPTYYLRYPPEHRKFIP
                     DCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGKQDMFDTAMLYLSDHGESLG
                     EKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMTCVAQRAEQGGFSHDNLFDS
                     LLGLMNVKTTVYQSQLDIFAPCRY"
                     /name="phosphoethanolamine--lipid A transferase MCR-4.3"
     gene            complement(9574..9861)
                     /locus_tag="H1R18_RS25105"
                     /old_locus_tag="H1R18_25085"
     CDS             complement(9574..9861)
                     /locus_tag="H1R18_RS25105"
                     /old_locus_tag="H1R18_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_172691904.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(9854..10171)
                     /locus_tag="H1R18_RS25110"
                     /old_locus_tag="H1R18_25090"
     CDS             complement(9854..10171)
                     /locus_tag="H1R18_RS25110"
                     /old_locus_tag="H1R18_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_040113353.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10418..10831)
                     /locus_tag="H1R18_RS25115"
                     /old_locus_tag="H1R18_25095"
     CDS             complement(10418..10831)
                     /locus_tag="H1R18_RS25115"
                     /old_locus_tag="H1R18_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(10839..11789)
                     /locus_tag="H1R18_RS25120"
                     /old_locus_tag="H1R18_25100"
     CDS             complement(10839..11789)
                     /locus_tag="H1R18_RS25120"
                     /old_locus_tag="H1R18_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_034167667.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     ARG             7612..9233
                     /name="mcr-4.3"
                     /gene="mcr-4.3"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-4.3"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(10840..11015)
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          208..1301
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctttctgctg cccattgttc ctgtttttcg cttttactca tgcccgttta ccctctgagt
       61 tttaacgggg gaattatgct cattgatggc agtggaggtc aaattcaact acaatccctg
      121 ataactcttt tatttatcag ggcgcactta gacgtttctt cacttcgttc agaaacggtg
      181 cgcgccttca gcgggataaa aacacgatgg cctcctatca cctcagcgta aaaaccggcg
      241 ggaaaggcag tgcctctccc catgccgatt atatttcgcg tgagggaaag tatgcccgtg
      301 aaaaagactc cgaccttgag cacaaggagt ccggcaatat gcctgcctgg gcagcgcata
      361 aaccgacgga gttctggaaa gccgctgaca cgtttgaacg tgccaacggc tgtacctacc
      421 gtgaaatcga gattgccctg ccgcgtgagc tgaaacccga acaacgcctt gaactggtcc
      481 gcgattttgt ccggcaggaa atcggcgacc gtcatgcgta tcagttcgcc attcataatc
      541 cgaaagcggc gatagccggc ggcgagcagc cgcacgcgca tatcatgttc tctgagcgga
      601 tgaatgacgg tatcgaccgt gacccgcagc agtattttaa gcgagctaac agcaaagccc
      661 ctgagcgcgg cggtgcgaaa aaggcacgtt tcggggaaac gccgacagaa cgcaaagagc
      721 acctcgtcgc ccagcgggaa cgctgggctg acctacagaa taagcacctt gaacggtatc
      781 agcataccga ccgcgttgat gcacgctcac tcaaagctca gggcattgag cgtgagcctg
      841 agcggcatct tggcgcggga caggttcagc ggttcgatac tgcgcagtta caggccatcc
      901 ttgagcgcag ggacgctgaa aggcaggcgg agcagtctcg tgatgaacgt gacagtgtga
      961 ttgacgtgac cacctcgcta cgggaagcca tcagcgagcg ggataacctg acgataaaac
     1021 aagagcagaa gcctgaacct gcgcaggaag acacttccgg cagggttttc gattttgaga
     1081 aagagccgga gaagctgaac gcactggtca gtgatgcgct gaaggacgta caggacgaaa
     1141 tcgaccttca gtcccttgtg aacgagacaa tggcggagtt tcaggggata catcaggaaa
     1201 tggagcgact ggcagaaaaa cagcgtcagc aggagaaaga acagcagaga ctcgccgaac
     1261 agacacgaca gaagccggat aaaggctggt cattttcacg atagacagga tttttcatga
     1321 gcgacgaaat taaacaaatc gctgccctca tcgggcatca tcaggcgctg gaaaagcgtg
     1381 ttacctctct gactgagcag tttcaggacg cctcagcaca attacagcag cagagtgaca
     1441 cgctgagcag gctcattcgt gagctggaca gcgcgtccgg taatatgact gaaaccgtca
     1501 ggcaatctgt tcatgccgcg ctcattcagg ttgagaaaga gctgaaacaa gccggattag
     1561 cacagcagaa accggcgaca gaagcgctta atcaggctac cgatacggcg aaggcgatga
     1621 tccatgaaat gcgacgcgag atgtcgcgct atacgtggaa atccgcgatt tatctcgtgc
     1681 tgacgatatt tttcgtcatg gctgcctgcg tgacggcatt tacgtggttt atgaatgacg
     1741 ggtacagcca gattgctgaa atgcaacgga tggaagcggt ctggcagaag aaagccccgc
     1801 tggctgatat atcgcgttgt gacggaaagc cctgcgttaa ggtggacacc cgagctacgt
     1861 atggtgataa agagaatacc tggatgatta tcaaaaaata acataacggc gcacattgcg
     1921 ccgttattgt tgtcgtgaat tagggggtca gtttggatat agagaattat tgtactataa
     1981 ggacttcccc ggcatgtcaa agccggtatt tatggtttct gtctgctctt cagtaaaaat
     2041 aaaggggaga gactgcggta ctataaacgg ccatgatgaa tcgtcatcga ttcgggccct
     2101 gatgaaagac gcgcgtgagg ttcttgttcg ttaagcggat acgtatatcc tgtacaatag
     2161 ccagataggc ctcacggggt ctaacccttt atcatcaacg aattgcaggt cagatggtta
     2221 tacagcttcg agctttagtt gactgagtac agccggaaat catcaccgta atgtagtgat
     2281 gcccaacaca gccgcgcatt tttggcggcg atggccacaa cagcccgcca gtaccctctg
     2341 cgctcaacca acatacaaac ccaacggctg aatgagtcag tccttttccc ggcgccaata
     2401 agcactgaac gagccccctg aaccagaagc gttcgcagat acgaatcacc ggcttttgtg
     2461 atcctgccga gtttcgactt tcccccgctg ctgtattgtg atggcgtcag ccccagccag
     2521 gctgccagtt gacgtccatt tttaaaatca tgtgcattac cgatactggc gaccagtgca
     2581 caggccgttg tgggaccaac gccctttagc tccatcagcc gctgactgcg atgatctgtt
     2641 ttggctatgt gggacaaaat tcggtcatat tcagtgatgt tagcttcaat gcgatcaacg
     2701 tgttccatca aatcatcaac acattgctga acctgaagcg gtaaagagct cttctgcaca
     2761 gaaaccatgt ggcgcagggc atcagcactt tgtggcgcga tgacaccgaa ttcagatatc
     2821 aatcctcgca gacgattata cgttgctgtt ttctcttcga taaaaccctg acgggtacga
     2881 tgtaaacact gcatggcctg ctggctttcg tctttcactg gtacgaaccg catatgcgga
     2941 cgacgaacag cttcacagat agcctgagca tcagctgcat cattttttcc tgattttccg
     3001 gccatacgat aaggcgatac aaacttagca gccatcagac gtggctcatg accatactgc
     3061 cgaaacaatc ttgcccagta gtgagccccg gagcatgctt ccatcccgat aacacaggga
     3121 ggcaaacttg caattaactc aggaagtgca gcacgcgata ctttaggctt aaccagaaca
     3181 gttctaccat tttgatcaac accgtgaaca gcaaacacat ttttagcaag atcgataccg
     3241 acagtcgtga tagtcataac gaattcctcc gggacgatgt gtaccccatg attgcacaag
     3301 agttaatcag gcgcataatc aggggaagtc ccttccattc gttaagcaca ttatttaacc
     3361 aattcggata cgtaagtgat tgatttatcg tcagtggcta tcgcttttag gttgtcgctc
     3421 ttgctgtatc gcatttaggt tatccgatta agacaaaatc ccctgaaaac gctgtatagc
     3481 gcgggagtac accctattgc tataggtaag tctgttcaaa aaacaggctt accgaatgga
     3541 agggacttcc cctgattatg cgcctgatta actcttgtgc aatcatgggg tacacatcgt
     3601 cccggaggaa ttcgttatga ctatcacgac tgtcggtatc gatcttgcta aaaatgtgtt
     3661 tgctgttcac ggtgttgatc aaaatggtag aactgttctg gttaagccta aagtatcgcg
     3721 tgctgcactt cctgagttaa ttgcaagttt gcctccctgt gttatcggga tggaagcatg
     3781 ctccggggct cactactggg caagattgtt tcggcagtat ggtcatgagc cacgtctgat
     3841 ggctgctaag tttgtatcgc cttatcgtat ggccggaaaa tcaggaaaaa atgatgcagc
     3901 tgatgctcag gctatctgtg aagctgttcg tcgtccgcat atgcggttcg taccagtgaa
     3961 agacgaaagc cagcaggcca tgcagtgttt acatcgtacc cgtcagggtt ttatcgaaga
     4021 gaaaacagca acgtataatc gtctgcgagg attgatatct gaattcggtg tcatcgcgcc
     4081 acaaagtgct gatgccctgc gccacatggt ttctgtgcag aagagctctt taccgcttca
     4141 ggttcagcaa tgtgttgatg atttgatgga acacgttgat cgcattgaag ctaacatcac
     4201 tgaatatgac cgaattttgt cccacatagc caaaacagat catcgcagtc agcggctgat
     4261 ggagctaaag ggcgttggtc ccacaacggc ctgtgcactg gtcgccagta tcggtaatgc
     4321 acatgatttt aaaaatggac gtcaactggc agcctggctg gggctgacgc catcacaata
     4381 cagcagcggg ggaaagtcga aactcggcag gatcacaaaa gccggtgatt cgtatctgcg
     4441 aacgcttctg gttcaggggg ctcgttcagt gcttattggc gccgggaaaa ggactgactc
     4501 attcagccgt tgggtttgta tgttggttga gcgcagaggg tactggcggg ctgttgtggc
     4561 catcgccgcc aaaaatgcgc ggctgtgttg ggcatcacta cattacggtg atgatttccg
     4621 gctgtactca gtcaactaaa gctcgaagct gtataaccat ctgacctgca attcgttgat
     4681 gataaagggt tagaccccgt gaggcctatc tggctattgt acaggatata cgtatccgct
     4741 taacgaacaa gaacctcacg cgcgtctttc atcagggccc gaatcgatga cgattcatca
     4801 tggccgttta tagtaccgca gtctctcccc tttattttta ctgaagagca gacagaaacc
     4861 ataaataccg gctttgacat gccggggaag tccttatagt acaaaaattt ccgatctcca
     4921 aactgacccc ttgatggccg ttttgttctt gcgtggatgc tgtttcaagg ttcttacctt
     4981 gccggggcgc tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg
     5041 cgccccttgg tagccggcat cggctgagac aaattgctcc tctccatgca gcagattacc
     5101 cagctgattg aggtcatgct cgttggccgc ggtggtgacc aggctgtggg tcaggccact
     5161 cttggcatcg acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt
     5221 ctgatgcatc tccggatcgc gttgctgctc tttgttcttg gtcgagctgg gtgcctcaat
     5281 gatggtggca tcgaccaagg tgccttgagt catcatgacg cctgcttcgg ccagccagcg
     5341 attgatggtc ttgaacaatt ggcgggccag ttgatgctgc tccagcaggt ggcggaaatt
     5401 catgatggtg gtgcggtccg gcaaggcgct atccagggat aaccgggcaa acagacgcat
     5461 ggaggcgatt tcgtacagag catcttccat cgcgccatcg ctcaggttgt accaatgctg
     5521 catgcagtga atgcgtagca tggtctccag cggatagggc cgtcggccat tgcccgcctt
     5581 gggataaaac ggctcgatga cagcggtcat attctgccat ggcagaatct gctccatgcg
     5641 ggagaggaaa atctcttttc gggtctgacg gcgcttagtg ctgaattcac tatcggcgaa
     5701 ggtgagttga tggctcatga tgtccctctg ggatgcgctc cggatgaaca tgatgatctc
     5761 atatcaggaa cttgttcgca ccttccctgt ctatcgcaca tttttgcaaa tcccgtcgat
     5821 tatttatttc catatgaaac tgaaatccgt agcatagttt gacgaaaaga acaggccatc
     5881 aggatgaaaa tggcccgggt aacaagccac cacctgaaac ggatgtcagc ctctaggagc
     5941 tgtaacctaa gggcggtagc gtgcctgcgt caggccactt gttctgaaaa accgagcgtc
     6001 ccgcccccca accattacag atcagacaaa gggctacgta ccccattggc gccgttttgc
     6061 agcacatggg tataaatttg tgtggttcga acatcagaat ggcccagttg ctcctgcacg
     6121 gttcgaatat ccgccccccg cgccagcaaa tgggtggcaa aggaatggcg caaggtatgg
     6181 caggtgaccg gttttcccag ctgcaactga cgcgccgtgg cacgaaccgc tttttgcagg
     6241 gtggttggat ccagatgatg tcgacgcaag gccccatcga caggatcgcg actcagatgg
     6301 gatgccggaa acaggtagtg ccaacccagc tgccccagca aaacacattt cgcgtgtggt
     6361 ggctggtggc tatataacaa agaacttgtc cgctgactta taggggttgt aagccggaac
     6421 cgccgaaatt tccgttagcc agaggggcgg cactgttgca aagttagcga tgaggcagcc
     6481 ttttgtctta ttcaaaggcc ttacatttca aaaactctgc ttaccaggcg catttcgccc
     6541 aggggatcac cataataaaa tgctgaggcc tggcctttgc gtagtgcacg catcacctca
     6601 atacctttga tggtggcgta agccgtcttc atggatttaa atcccagcgt ggcgccgatt
     6661 atccgtttca gtttgccatg atcgcattca atcacgttgt tccggtactt aatctgtcgg
     6721 tgttcaacgt cagacgggca ccggccttcg cgtttgagca gagcaagcgc gcgaccatag
     6781 gcgggcgctt tatccgtgtt gatgaatcgc gggatctgcc acttcttcac gttgttgagg
     6841 attttaccca gaaaccggta tgcagctttg ctgttacgac gggaggagag ataaaaatcg
     6901 acagtgcggc cccggctgtc gacggcccgg tacagatacg cccagcggcc attgaccttc
     6961 acgtaggttt catccatgtg ccacgggcaa agatcggaag ggttacgcca gtaccagcgc
     7021 agccgttttt ccatttcagg cgcataacgc tgaacccagc ggtaaatcgt ggagtgatcg
     7081 acattcactc cgcgttcagc cagcatctcc tgcagctcac ggtaactgat gccgtatttg
     7141 cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg
     7201 ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca
     7261 acagtgcctg taaaactcac ggatacctta aaacaggcta tttatgataa aagagctgag
     7321 ggggccacca ttgggcagct tgccaaggag tatcaccttg gagaagcctc tatttatcgg
     7381 gcactgaatt ctgtgaagag cacacaagaa attacaagta aacttataag taattgaagt
     7441 ttatttactt aattgtttac atgtaacttt taggtgaatt gggctcaatt cacctaaaac
     7501 ggtcttatct gttaagtttg ttggtgactc attaaaataa ccttttaatc ataaggttat
     7561 tttaatgagg tcaagctagt attgtttttt ttagcaaaaa aggggttttt gtgatttcta
     7621 gatttaagac gttatcggtt aaccaattca ctttcatcac tgcgttgttt tatgttgcca
     7681 ttttcaatct accgctcttt ggtatagtgc gaaaaggaat tgaaaaacaa ccagaagttg
     7741 atcccctttt catcgcatct atgccgctat ttttaacatt tgcgctgagt tttttgtttt
     7801 caatttttac cgtcaaatac ctgctgaagc ccttttttat cgtattgacg ttactttcct
     7861 caagtgtatt ttttgcagcc tatcaataca atgtcgtgtt tgactacggc atgatagaaa
     7921 acacgtttca aacacatcct gctgaagcat tgatgtatgt aaatcttgca tcaattacca
     7981 atctactgct gactgggcta ttaccgtcat atcttattta taaggccgat attcattatc
     8041 agcccttttt taaggagtta ttgcataaat tagcctttat gctgctaatg ttcgttggca
     8101 ttgggatagt cgcctttttt tactatcaag attatgctgc atttggtcga aacaacagtg
     8161 agttaaggcg ttacattgtc cctacctatt ttgtcagtag tgcatctaaa tatctcaatg
     8221 agcactattt gcagacgccc atggaatacc aacaacttgg cctagatgcg aagaatgcca
     8281 gtcgtaaccc gaacactaaa cctaacttat tagtgtttgt tgtgggtgaa actgcgcgct
     8341 caatgagcta tcaatattat ggatataaca agccaaccaa tgctcatacc caaaatcagg
     8401 ggctgattgc gtttaacgat actagctcat gcggcacggc cacggcggtg tctctaccct
     8461 gtatgttttc acgaatgggg cgggcagact atgatcctcg ccgtgctaat gctcaagaca
     8521 cagtgattga tgtgttaagt catagtggta taaaagtaca gtggtttgat aatgattctg
     8581 gctgtaaagg tgtgtgtgat caggttgaaa atctcacgat agatttgaag agtgatccga
     8641 agctgtgttc tggccaatat tgttttgacc aagtattgct caacaaatta gataaaattc
     8701 tggcagtagc accaagtcaa gatacagtaa tttttttgca tatcattggt agtcatggac
     8761 caacttatta tcttagatac ccgccagagc atcgtaaatt tataccggat tgtccgcgca
     8821 gtgatattca aaattgcagt caagaagaac tgattaacac ctacgacaac actattctat
     8881 atacggattt tattctcagt gaagtggtga ataaattaaa aggtaagcag gatatgttcg
     8941 atactgcaat gctgtatctc tctgaccatg gtgagtcttt gggtgaaaag ggcatgtatt
     9001 tacatggtgc gccctatagt attgcaccga aagaacaaac tagcgtacca atgctggctt
     9061 gggtatctaa tgactttagc caagataatc agttgaacat gacttgtgtt gcacagcgag
     9121 cagaacaggg cggcttttcc cacgacaatt tgttcgacag tttgctagga cttatgaatg
     9181 taaaaaccac cgtctatcag agccaactcg atatttttgc accttgcagg tattagatat
     9241 ggctgtaaat gttttatctt aggggtgaca tattcacccc ttaaatcttg gactaacaat
     9301 gtcctctatt gttacattta gctctcaatc ttccacaatg agttatcacg gtcttttaga
     9361 cttaacatca atttgccgtt gtgaaactag aacaatggct tacaagctgt aaatgctaac
     9421 tttacatttt tggcgcgttg ggcaaaatta gcctatttat atcccttagg attatctcat
     9481 atgtgtgaaa tgatggtttt ttgcaaaaaa actccttgtt caacataagc gatcttgcac
     9541 gcatcaaaag gctgcttaaa gcggccttga aaattacaac gtgatctgag ggtgcttaac
     9601 accgcatgct cttgcgtatc gagccagtgt ttcaatgcta gccttaatca cattagattc
     9661 catgcgagaa acggtaggtg gagtaacacc catgcgttct gccacctggg ctcttgttaa
     9721 tcctgcgtga tgacgccatt cagcgagcat agcttgaagt ctttccttgc gctcctctgc
     9781 ttcataggct gcttgaacct caggattaga catcaattca ttcctaacat gctcccaagc
     9841 gataccttta actttactca tttagcatct ccttaagacg ttcaaaggcc atagatattt
     9901 cacccgatgg ggttttctga gtctttttaa taaagactct aagtagatag atattcctgc
     9961 ctttctgata aacataaaga cctctggcaa tatccgtccc catcgtcctt aactcaaata
    10021 atccatcccg gagtggtttg ctatctggct ctcgtaatgc agtagcgttg gttttaagtt
    10081 tatcaatcag gcgaatcatc ttcgctctga ctactggcgg taagccagcc agctcatttg
    10141 gtacatcatc gtgcaatatg acgttaaaca ctggttcgcc ctccattgcg aaatattagc
    10201 ataaaaagca atttgcgtta tagggtaatt ttatggtttt tctgctgtat ttaacataag
    10261 cgatcttgcg cgcaccgccc gaacaccact cgccacaaaa acccgccgga acgtccaaaa
    10321 gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt tatcagaatc
    10381 gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag aattgccatg
    10441 attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt gattcgataa
    10501 gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca agttgtctca
    10561 ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt ctattaggtg
    10621 ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct ttgaatgcac
    10681 caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata
    10741 tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg tttgttagtc
    10801 ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc
    10861 agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga
    10921 tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt
    10981 gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtggga atctgatgta
    11041 atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg
    11101 agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc
    11161 ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa
    11221 acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta
    11281 aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt
    11341 aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga
    11401 ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact
    11461 aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct
    11521 ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct
    11581 aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg
    11641 aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca
    11701 cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca
    11761 tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca
    11821 ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct ttgagttgtg
    11881 ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct
    11941 gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat
    12001 agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca
    12061 ctactttagt cagttccgca gtattaaaaa ggatgtcgca aacgctgttt gctcctctac
    12121 aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc
    12181 tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt
    12241 cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcctt
    12301 ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac gggcttctca
    12361 gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt tcagcagttc
    12421 ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt cattcagact
    12481 ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct tactgtcaac
    12541 cagacccgcc aggataagca atccggcaga ctggtacaga gcatggtcac gggctttacg
    12601 ggcggctctg gcttcggctc gcttttctgc ctgtatcagg ttcatgactt tcgctctggc
    12661 ttcctgcgct ttctctgctg cctgttcagc cttcatcagc agggacagcg ttttgatatc
    12721 ctgtgctgtt ttatctgctt tatccgtcag tatcagcata agtttctgtt gctcattcgg
    12781 cgactttaag ccgcgaatat actgcact
//
