LOCUS       NZ_CP059423             2493 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain ENCL49790 plasmid p49790_COL,
            complete sequence.
ACCESSION   NZ_CP059423
VERSION     NZ_CP059423.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15589938
            Assembly: GCF_013873945.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Chudejova,K., Mattioni
            Marchetti,V. and Hrabak,J.
  TITLE     Detection of Five mcr-9-Carrying Enterobacterales Isolates in Four
            Czech Hospitals
  JOURNAL   mSphere 5 (6), e01008-20 (2020)
   PUBMED   33298573
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 490.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013873945.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:37:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,285
            CDSs (total)                      :: 5,169
            Genes (coding)                    :: 5,070
            CDSs (with protein)               :: 5,070
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 36 of 99
            Pseudo Genes (incomplete)         :: 64 of 99
            Pseudo Genes (internal stop)      :: 12 of 99
            Pseudo Genes (multiple problems)  :: 13 of 99
            Pseudo Genes (short protein)      :: 1 of 99
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059423.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="ENCL49790"
                     /isolation_source="decubitus swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p49790_COL"
                     /geo_loc_name="Czech Republic: Ceske Budejovice"
                     /lat_lon="48.97 N 14.48 E"
                     /collection_date="2019"
                     /collected_by="ceske budejovice hospital"
     gene            join(2346..2493,1..71)
                     /locus_tag="H1Z82_RS23595"
                     /old_locus_tag="H1Z82_23595"
     CDS             join(2346..2493,1..71)
                     /locus_tag="H1Z82_RS23595"
                     /old_locus_tag="H1Z82_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(362..547)
                     /locus_tag="H1Z82_RS23600"
                     /old_locus_tag="H1Z82_23600"
     CDS             complement(362..547)
                     /locus_tag="H1Z82_RS23600"
                     /old_locus_tag="H1Z82_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(913..1140)
                     /locus_tag="H1Z82_RS23605"
                     /old_locus_tag="H1Z82_23605"
     CDS             complement(913..1140)
                     /locus_tag="H1Z82_RS23605"
                     /old_locus_tag="H1Z82_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          228..315
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1556..2084)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ccgaaccggc cgtaaggact gtcatgctcg aatttcttga atccgttcgc attcattaca
       61 tttcgtcctg agcattttct gtatttctgt ccatacttaa acgaggcggc cgtcacggac
      121 ggtggtcgtc gttccatgat gctgttatgg acgagtgaga gtaatctaat ctaacttgat
      181 tgagttccgg gcgtgccgcc cggttttttt tgctgtttcc cggtgtccgt tccgggacag
      241 gatgtgcatt tgtaataccg cgcacgcgcc ggcatcacaa ataccctggt cagggctgcg
      301 ccccgacacc ccgccatcgc ctcacggccg cgcactgaat tgcgcacccg ttcaccgatt
      361 ttcacgcatt ttcctcgcct atctgtgcgt tcagcatggc gtaaagcgct tcggcctgat
      421 cgtgcaggtg ctcacagcag ccggcagcct catccagatc catctgctcg agccgctcaa
      481 ggaggagaag tgtctgtgcg cggataaatt tggccatatt gagggcggcg gattcctgtc
      541 tggtcactgg tgattttccc cgtctggtga tgatttttgc gtgaaaagag catggcgtca
      601 ttctggtgca tagtcatgca tgccgttaaa atttaacagg cgcgtttctg acgggttccg
      661 gggtggtttg ttgtggtttt tgtcatggtt tcgtcaggaa cgcgctgaac gcgtctcagg
      721 cggtgcgggc aacggatgtt atggtaaatt cttgggggct tgattataaa ataccgcact
      781 tcctcttatg tcttgtgctt tttagacaac cctgaaagct tttagctttg gtgatgcaat
      841 ttgctgggat tttggcggag aaccacaggt aaagaaaaag gccacattag cggccttttt
      901 cggagaagat gctcagtgcg gaatttcgga gacttcttta acgtcggttt tattgatctg
      961 ctgtttgaca ccattcgcgt ctgtatagcc aagcaggcct gagtctgatt ctttaggttt
     1021 gccgtcgctg acaatagtgc gaccgtcatt ggtgtgtatg gcatagcttg tgcgcgtgca
     1081 gccggtgagg gctgaaagcg caactgccgc tgcgaacact gaaacaaaaa tctttttcaa
     1141 agccagctcc ttttatccat atcagttttg gtcactaacc atctaactat aggtcatttt
     1201 ttatgtgaaa agaggcttga tggtgggctg tatatcgagt taggcgcgag gtgctatggt
     1261 caaagtgtgg tgtcaggcgg ggaggaggct tatatgagat gtaatccggc cgatacatat
     1321 accaagatga tttaagtatt cctctttgtg aggttggatg tctaatttat aaaggatctt
     1381 cttgagatcc tttttttccg cgcgtaatct cttgccctgt aaacgaaaaa accaccctgg
     1441 caggtggttt ttcgaaggtt aggtaatcct ggcagatccc ctaaccgtgg taacagtctt
     1501 gtgcgagaca tgtcaccaaa tttgtccttt cagtgtagcc tcactaaggc cgccacttca
     1561 agaactcttg agacatctct cgcacatcct gtttgccaat ggccgttgcc aatggcgatt
     1621 agtcgtgtct ttccgggttg gactcaagtt gatagttacc ggataaggcg cagcggtcgg
     1681 actgaacggg gggttcgtgc atacagtcca tcctggagcg aactgccttc ccggaactga
     1741 gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg agtgacaccg gtaaaccgaa
     1801 aggcaggaat gcgggggagc acgagggagc caccaggggg aaacgcctgg tatctttaag
     1861 ccgcatcggg tttcgccacc actgatttga gcgtcagatt ctgtgatgct tgtcaggggg
     1921 gcggagccta tggaaaaacg gctttggcgc ggccttatgc tttcttcgtt aagtatcttc
     1981 ctggcatccc ccaggaaatt tctgatccat ccgtaagccc gtcccgctcg ccgcagccga
     2041 acgaccgagc ggagcgagtc agtgagcgag gaagcggaat atattctgta tcacattttc
     2101 tcctgacgcg ttttctttca ctttctgcgc ctgtcttatg tggcattaat gctatgtgtt
     2161 actgccatgc tacatcttaa gccagtatac actccgctag tgctccgtga ctggtccggc
     2221 gctgcgcccg gaacccgcct gtaccggttc agcagccgtt ccggcctgac tgcaattttt
     2281 tttttcatcc ctgcccgcta ccctgtaaac ctttcttctg cgttgccgtt aacagtaggt
     2341 aagccatgtt tggtttttct tccggagccc tgatgactct tagtgatgat gagcgacacc
     2401 tgcttgtgtc cgttgtttcg gtctggctac ggcgtgccgg aggggatgcc ggagccatga
     2461 tgctggatgc ataccggcag attttgtctg aaa
//
