LOCUS       NZ_CP059420             2494 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Enterobacter hormaechei strain ENCL48880 plasmid p48880_COL,
            complete sequence.
ACCESSION   NZ_CP059420
VERSION     NZ_CP059420.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15589926
            Assembly: GCF_013873515.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Chudejova,K., Mattioni
            Marchetti,V. and Hrabak,J.
  TITLE     Detection of Five mcr-9-Carrying Enterobacterales Isolates in Four
            Czech Hospitals
  JOURNAL   mSphere 5 (6), e01008-20 (2020)
   PUBMED   33298573
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 386.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/07/2024 12:12:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,703
            CDSs (with protein)               :: 4,703
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 58 of 133
            Pseudo Genes (incomplete)         :: 83 of 133
            Pseudo Genes (internal stop)      :: 33 of 133
            Pseudo Genes (multiple problems)  :: 34 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059420.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="ENCL48880"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p48880_COL"
                     /geo_loc_name="Czech Republic: Kolin"
                     /lat_lon="50.02 N 15.20 E"
                     /collection_date="2019"
                     /collected_by="Nemocnice Kolin"
     gene            1098..1316
                     /locus_tag="H1D58_RS23260"
                     /old_locus_tag="H1D58_23260"
     CDS             1098..1316
                     /locus_tag="H1D58_RS23260"
                     /old_locus_tag="H1D58_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1607..1792)
                     /locus_tag="H1D58_RS23265"
                     /old_locus_tag="H1D58_23265"
     CDS             complement(1607..1792)
                     /locus_tag="H1D58_RS23265"
                     /old_locus_tag="H1D58_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2157..2384)
                     /locus_tag="H1D58_RS23270"
                     /old_locus_tag="H1D58_23270"
     CDS             complement(2157..2384)
                     /locus_tag="H1D58_RS23270"
                     /old_locus_tag="H1D58_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1473..1560
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(306..834)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 gtgctatggt caaagtgtgg tgtcaggcgg ggaggaggct tatatgagat gtaatccggc
       61 cgatacatat accaagatga tttaagtatt cctctttgtg aggttggatg tctaatttat
      121 aaaggatctt cttgagatcc tttttttccg cgcgtaatct cttgccctgt aaacgaaaaa
      181 accaccctgg caggtggttt ttcgaaggtt aggtaatcct ggcagatccc ctaaccgtgg
      241 taacagtctt gtgcgagaca tgtcaccaaa tttgtccttt cagtgtagcc tcactaaggc
      301 cgccacttca agaactcttg agacatctct cgcacatcct gtttgccaat ggccgttgcc
      361 aatggcgatt agtcgtgtct ttccgggttg gactcaagtt gatagttacc ggataaggcg
      421 cagcggtcgg actgaacggg gggttcgtgc atacagtcca tcctggagcg aactgccttc
      481 ccggaactga gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg agtgacaccg
      541 gtaaaccgaa aggcaggaat gcgggggagc acgagggagc caccaggggg aaacgcctgg
      601 tatctttaag ccgcatcggg tttcgccacc actgatttga gcgtcagatt ctgtgatgct
      661 tgtcaggggg gcggagccta tggaaaaacg gctttggcgc ggccttatgc tttcttcgtt
      721 aagtatcttc ctggcatccc ccaggaaatt tctgatccat ccgtaagccc gtcccgctcg
      781 ccgcagccga acgaccgagc ggagcgagtc agtgagcgag gaagcggaat atattctgta
      841 tcacattttc tcctgacgcg ttttctttca ctttctgcgc ctgtcttatg tggcattaat
      901 gctatgtgtt actgccatgc tacatcttaa gccagtatac actccgctag tgctccgtga
      961 ctggtccggc gctgcgcccg gaacccgcct gtaccggttc agcagccgtt ccggcctgac
     1021 tgcaattttt tttttttcat ccctgcccgc taccctgtaa acctttcttc tgcgttgccg
     1081 ttaacagtag gtaagccatg tttggttttt cttccggagc cctgatgact cttagtgatg
     1141 atgagcgaca cctgcttgtg tccgttgttt cggtctggct acggcgtgcc ggaggggatg
     1201 ccggagccat gatgctggat gcataccggc agattttgtc tgaaaccgaa ccggccgtaa
     1261 ggactgtcat gctcgaattt cttgaatccg ttcgcattca ttacatttcg tcctgagcat
     1321 tttctgtatt tctgtccata cttaaacgag gcggccgtca cggacggtgg tcgtcgttcc
     1381 atgatgctgt tatggacgag tgagagtaat ctaatctaac ttgattgagt tccgggcgtg
     1441 ccgcccggtt ttttttgctg tttcccggtg tccgttccgg gacaggatgt gcatttgtaa
     1501 taccgcgcac gcgccggcat cacaaatacc ctggtcaggg ctgcgccccg acaccccgcc
     1561 atcgcctcac ggccgcgcac tgaattgcgc acccgttcac cgattttcac gcattttcct
     1621 cgcctatctg tgcgttcagc atggcgtaaa gcgcttcggc ctgatcgtgc aggtgctcac
     1681 agcagccggc agcctcatcc agatccatct gctcgagccg ctcaaggagg agaagtgtct
     1741 gtgcgcggat aaatttggcc atattgaggg cggcggattc ctgtctggtc actggtgatt
     1801 ttccccgtct ggtgatgatt tttgcgtgaa aagagcatgg cgtcattctg gtgcatagtc
     1861 atgcatgccg ttaaaattta acaggcgcgt ttctgacggg ttccggggtg gtttgttgtg
     1921 gtttttgtca tggtttcgtc aggaacgcgc tgaacgcgtc tcagcggtgc gcgtaacgga
     1981 tgttatggta aattcttggg ggcttgatta taaaataccg cacttcctct tatgtcttgt
     2041 gctttttaga caaccctgaa agcttttagc tttggtgatg caatttgctg ggattttggc
     2101 ggagaaccac aggtaaagaa aaaggccaca ttagcggcct ttttcggaga agatgctcag
     2161 tgcggaattt cggagacttc tttaacgtcg gttttattga tctgctgttt gacaccattc
     2221 gcgtctgtat agccaagcag gcctgagtct gattctttag gtttgccgtc gctgacaata
     2281 gtgcgaccgt cattggtgtg tatggcatag cttgtgcgcg tgcagccggt gagggctgaa
     2341 agcgcaactg ccgctgcgaa cactgaaaca aaaatctttt tcaaagccag ctccttttat
     2401 ccatatcagt tttggtcact aaccatctaa ctataggtca ttttttatgt gaaaagaggc
     2461 ttgatggtgg gctgtatatc gagttaggcg cgag
//
