LOCUS       NZ_CP059415             2494 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain ENCL48212 plasmid p48212_COL,
            complete sequence.
ACCESSION   NZ_CP059415
VERSION     NZ_CP059415.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15589925
            Assembly: GCF_013873655.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Chudejova,K., Mattioni
            Marchetti,V. and Hrabak,J.
  TITLE     Detection of Five mcr-9-Carrying Enterobacterales Isolates in Four
            Czech Hospitals
  JOURNAL   mSphere 5 (6), e01008-20 (2020)
   PUBMED   33298573
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbial Assembly v. SMRT Link v.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 290.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013873655.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:42:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,172
            Genes (coding)                    :: 5,070
            CDSs (with protein)               :: 5,070
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 39 of 102
            Pseudo Genes (incomplete)         :: 65 of 102
            Pseudo Genes (internal stop)      :: 13 of 102
            Pseudo Genes (multiple problems)  :: 15 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="ENCL48212"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p48212_COL"
                     /geo_loc_name="Czech Republic: Tabor"
                     /lat_lon="49.41 N 14.64 E"
                     /collection_date="2019"
                     /collected_by="Nemocnice Tabor"
     gene            join(2341..2494,1..65)
                     /locus_tag="H1D56_RS25220"
                     /old_locus_tag="H1D56_25220"
     CDS             join(2341..2494,1..65)
                     /locus_tag="H1D56_RS25220"
                     /old_locus_tag="H1D56_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(356..541)
                     /locus_tag="H1D56_RS25225"
                     /old_locus_tag="H1D56_25225"
     CDS             complement(356..541)
                     /locus_tag="H1D56_RS25225"
                     /old_locus_tag="H1D56_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(907..1134)
                     /locus_tag="H1D56_RS25230"
                     /old_locus_tag="H1D56_25230"
     CDS             complement(907..1134)
                     /locus_tag="H1D56_RS25230"
                     /old_locus_tag="H1D56_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          222..309
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1550..2078)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 cggccgtaag gactgtcatg ctcgaatttc ttgaatccgt tcgcattcat tacatttcgt
       61 cctgagcatt ttctgtattt ctgtccatac ttaaacgagg cggccgtcac ggacggtggt
      121 cgtcgttcca tgatgctgtt atggacgagt gagagtaatc taatctaact tgattgagtt
      181 ccgggcgtgc cgcccggttt tttttgctgt ttcccggtgt ccgttccggg acaggatgtg
      241 catttgtaat accgcgcacg cgccggcatc acaaataccc tggtcagggc tgcgccccga
      301 caccccgcca tcgcctcacg gccgcgcact gaattgcgca cccgttcacc gattttcacg
      361 cattttcctc gcctatctgt gcgttcagca tggcgtaaag cgcttcggcc tgatcgtgca
      421 ggtgctcaca gcagccggca gcctcatcca gatccatctg ctcgagccgc tcaaggagga
      481 gaagtgtctg tgcgcggata aatttggcca tattgagggc ggcggattcc tgtctggtca
      541 ctggtgattt tccccgtctg gtgatgattt ttgcgtgaaa agagcatggc gtcattctgg
      601 tgcatagtca tgcatgccgt taaaatttaa caggcgcgtt tctgacgggt tccggggtgg
      661 tttgttgtgg tttttgtcat ggtttcgtca ggaacgcgct gaacgcgtct caggcggtgc
      721 gggcaacgga tgttatggta aattcttggg ggcttgatta taaaataccg cacttcctct
      781 tatgtcttgt gctttttaga caaccctgaa agcttttagc tttggtgatg caatttgctg
      841 ggattttggc ggagaaccac aggtaaagaa aaaggccaca ttagcggcct ttttcggaga
      901 agatgctcag tgcggaattt cggagacttc tttaacgtcg gttttattga tctgctgttt
      961 gacaccattc gcgtctgtat agccaagcag gcctgagtct gattctttag gtttgccgtc
     1021 gctgacaata gtgcgaccgt cattggtgtg tatggcatag cttgtgcgcg tgcagccggt
     1081 gagggctgaa agcgcaactg ccgctgcgaa cactgaaaca aaaatctttt tcaaagccag
     1141 ctccttttat ccatatcagt tttggtcact aaccatctaa ctataggtca ttttttatgt
     1201 gaaaagaggc ttgatggtgg gctgtatatc gagttaggcg cgaggtgcta tggtcaaagt
     1261 gtggtgtcag gcggggagga ggcttatatg agatgtaatc cggccgatac atataccaag
     1321 atgatttaag tattcctctt tgtgaggttg gatgtctaat ttataaagga tcttcttgag
     1381 atcctttttt tccgcgcgta atctcttgcc ctgtaaacga aaaaaccacc ctggcaggtg
     1441 gtttttcgaa ggttaggtaa tcctggcaga tcccctaacc gtggtaacag tcttgtgcga
     1501 gacatgtcac caaatttgtc ctttcagtgt agcctcacta aggccgccac ttcaagaact
     1561 cttgagacat ctctcgcaca tcctgtttgc caatggccgt tgccaatggc gattagtcgt
     1621 gtctttccgg gttggactca agttgatagt taccggataa ggcgcagcgg tcggactgaa
     1681 cggggggttc gtgcatacag tccatcctgg agcgaactgc cttcccggaa ctgagtgtca
     1741 ggcgtggaat gagaaaccgc ggccataaca gcggagtgac accggtaaac cgaaaggcag
     1801 gaatgcgggg gagcacgagg gagccaccag ggggaaacgc ctggtatctt taagccgcat
     1861 cgggtttcgc caccactgat ttgagcgtca gattctgtga tgcttgtcag gggggcggag
     1921 cctatggaaa aacggctttg gcgcggcctt atgctttctt cgttaagtat cttcctggca
     1981 tcccccagga aatttctgat ccatccgtaa gcccgtcccg ctcgccgcag ccgaacgacc
     2041 gagcggagcg agtcagtgag cgaggaagcg gaatatattc tgtatcacat tttctcctga
     2101 cgcgttttct ttcactttct gcgcctgtct tatgtggcat taatgctatg tgttactgcc
     2161 atgctacatc ttaagccagt atacactccg ctagtgctcc gtgactggtc cggcgctgcg
     2221 cccggaaccc gcctgtaccg gttcagcagc cgttccggcc tgactgcaat tttttttttt
     2281 catccctgcc cgctaccctg taaacctttc ttctgcgttg ccgttaacag taggtaagcc
     2341 atgtttggtt tttcttccgg agccctgatg actcttagtg atgatgagcg acacctgctt
     2401 gtgtccgttg tttcggtctg gctacggcgt gccggagggg atgccggagc catgatgctg
     2461 gatgcatacc ggcagatttt gtctgaaacc gaac
//
