LOCUS       NZ_CP059341             2113 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain MS8320 plasmid pMS8320B, complete sequence.
ACCESSION   NZ_CP059341
VERSION     NZ_CP059341.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10141488
            Assembly: GCF_030991425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   White,R.T.
  TITLE     Escherichia coli ST1193 strain MS8320 plasmid pMS8320B
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   White,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2020) School of Chemistry and Molecular
            Biosciences, The University of Queensland, Building 76, Cooper Road,
            St Lucia, QLD 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Flye v. 2.5
            Assembly Name                     :: MS8320_CS12_NT018_ST1193
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 16:26:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,205
            CDSs (total)                      :: 5,086
            Genes (coding)                    :: 4,776
            CDSs (with protein)               :: 4,776
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 161 of 310
            Pseudo Genes (incomplete)         :: 179 of 310
            Pseudo Genes (internal stop)      :: 41 of 310
            Pseudo Genes (multiple problems)  :: 66 of 310
            Pseudo Genes (short protein)      :: 4 of 310
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059341.1.
            Bacteria and source DNA available from Escherichia coli.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS8320"
                     /serotype="O75:H5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS8320B"
                     /geo_loc_name="Australia: Northern Territory"
                     /collection_date="2012"
     gene            703..1668
                     /locus_tag="MS8320_RS26005"
                     /old_locus_tag="MS8320_B0001"
     CDS             703..1668
                     /locus_tag="MS8320_RS26005"
                     /old_locus_tag="MS8320_B0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1672..1938
                     /locus_tag="MS8320_RS26010"
                     /old_locus_tag="MS8320_B0002"
     CDS             1672..1938
                     /locus_tag="MS8320_RS26010"
                     /old_locus_tag="MS8320_B0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1097..1328
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aacactctgc gagtggtaca ttttccccgg attatcgtcc tgagcctgcc gctggctctc
       61 tttctaccgc ctcgctttgc tcgttgctca acgcctcaca gacacggatt aaaatccgca
      121 tccgttcacc gttttttaaa gtccgttaaa agcatgatgc catctccgag agttaatctc
      181 gtcaaatgct aaatcgtggg ggtccccttt ggggttccga tttagtgatt gacgacacca
      241 ccgattaaaa aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag
      301 acagggtttt gtttttattc cctgttttgg tgatcgggtg tgtggaaaag gttggggtaa
      361 gccgttcggg ggtgcttgtt ttggggggtt aaaattgtgg ttattttttg cgcaattctc
      421 gcgcgtgatc cttgtattta tacttaaggg ataaatggcg gatatgaaat agtggtttag
      481 cccagtaatg acgaggcttt gagtgggttt tgacaggtca aagaaaatgg agcagaattg
      541 aggcgttttt aatcggcgtt ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca
      601 cctcagcggc aaactctggt gacatgtact ggctcgcaat gcacaggtac gtgatgaata
      661 taccacatca aatcacagcc tgcccagatc ggagcaggct taatgtcaga agataaattc
      721 ctttcggact acagcccccg tgatgcagtt tgggataccc agcgcacgct taccgattct
      781 gtcgggggta tctaccagac tgctgctgaa ttcgagcgct atgcactccg tatggcctcc
      841 tgtagcggtt tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta
      901 cggcttcgta gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga
      961 accctcatgt ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg
     1021 tcttcccgat ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga
     1081 acagtcctta cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca
     1141 cctgttcagg ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat
     1201 ccgcattttc actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt
     1261 aagcacgaac gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat
     1321 atcgatattc gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa
     1381 ctgcaaagcg cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaac
     1441 gatcctgagt ggtttcttga gctgacgcgg cagcttcaca agcgccgttt tatctcgacc
     1501 ggtggggcgc taaaaaacgt cctccagttg gatcgagaaa ccaatgagga tcttgtcatt
     1561 gccgacgatg taggggatgg cactgatgac gggaagcgga cggcgtttgt ctgggattca
     1621 ggtaaacggc gttacaaacg cgcccctgag aaggataaat cggattaacg tatgaatatt
     1681 aatattgaat acctgaatgg aaataagact attggtttat tttttttaag aagtgaagcg
     1741 gtgattcctg acaggtttaa aaaccttatt ttgcttattg atggattaag ttttggcaca
     1801 tttggttttc atccgcacga aggttttgag gatgaattaa ttttatatat tcagaaaaca
     1861 aacgagaggg taaaaactct ttttgtgaaa atagatttaa acggtatcaa gtttggattt
     1921 ttaagaacgc attcttagtt ctggaaaaga gccagcggca ggctgaggtg ataggtacga
     1981 gattgcatgc aatctctagt gctctgtcta tcctgcatta tcctcagcat tatcctcagc
     2041 attatcctca gcattatcct cagccttgcc aactcgacac caatgcagga tagacaatcc
     2101 gatgtcaaat gat
//
