LOCUS       NZ_CP059334             5180 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain MS10860 plasmid pMS10860B, complete
            sequence.
ACCESSION   NZ_CP059334
VERSION     NZ_CP059334.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10141462
            Assembly: GCF_030991405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   White,R.T.
  TITLE     Escherichia coli ST410 strain MS10860 plasmid pMS10860B
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   White,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2020) School of Chemistry and Molecular
            Biosciences, The University of Queensland, Building 76, Cooper Road,
            St Lucia, QLD 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Flye v. 2.5
            Assembly Name                     :: MS10860_QU059_ST1193
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 177.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 16:40:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,074
            CDSs (total)                      :: 4,956
            Genes (coding)                    :: 4,697
            CDSs (with protein)               :: 4,697
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 110 of 259
            Pseudo Genes (incomplete)         :: 161 of 259
            Pseudo Genes (internal stop)      :: 43 of 259
            Pseudo Genes (multiple problems)  :: 50 of 259
            Pseudo Genes (short protein)      :: 4 of 259
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059334.1.
            Bacteria and source DNA available from Escherichia coli.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS10860"
                     /serotype="O75:H5"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS10860B"
                     /geo_loc_name="Australia: New South Wales"
                     /collection_date="2007"
     gene            693..902
                     /locus_tag="MS10860_RS25270"
                     /old_locus_tag="MS10860_B0001"
     CDS             693..902
                     /locus_tag="MS10860_RS25270"
                     /old_locus_tag="MS10860_B0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(968..1501)
                     /locus_tag="MS10860_RS25275"
                     /old_locus_tag="MS10860_B0002"
     CDS             complement(968..1501)
                     /locus_tag="MS10860_RS25275"
                     /old_locus_tag="MS10860_B0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1504..2430)
                     /locus_tag="MS10860_RS25280"
                     /old_locus_tag="MS10860_B0003"
     CDS             complement(1504..2430)
                     /locus_tag="MS10860_RS25280"
                     /old_locus_tag="MS10860_B0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2607..3005
                     /locus_tag="MS10860_RS25285"
                     /old_locus_tag="MS10860_B0004"
     CDS             2607..3005
                     /locus_tag="MS10860_RS25285"
                     /old_locus_tag="MS10860_B0004"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(3209..4123)
                     /locus_tag="MS10860_RS25290"
                     /old_locus_tag="MS10860_B0005"
     CDS             complement(3209..4123)
                     /locus_tag="MS10860_RS25290"
                     /old_locus_tag="MS10860_B0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          complement(3349..4123)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(1508..2430)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatagttcca ttagtttaaa gtgtcacaca aactgagcat ccatttgttt tccttacttt
       61 tgcccctgta ttccaagggg tttttttata acttaaagat tagcacacca cacaaaaaca
      121 ataaaaaaaa acgacaagtt atacaataaa accagtacta tccatacagc taattacata
      181 taaatactat taaaccatct ggacattaaa aataaactct tataacataa aattaatgcg
      241 gttccggtac catatgtcag ggataatgtg caaatggagg tatcggaact gcatttcagt
      301 tcattattaa ttaatgacaa aaaaaacctc tgcgagcgca gagggggtaa ggaatgttcc
      361 cggtcataat atgtcgatag tgtttatata catacagaac aagaacaaca accagccaac
      421 taataaatat tttatttttt agagctgaaa ccttaaagct cacttccatt ccatacattg
      481 ttataaacct tatgttgaaa actgaaagta agtacggcag acttattaag ttgcgtgcag
      541 tcttttcacg ttatacaaac aagaacacct gcgaagttat ttctccttac atatccccct
      601 atgccaacgg gggatttttt ctggttcact aacgcataaa taatagtgac accctatcat
      661 tcacaaaccg tgataatata aggattatca tcatggcaaa attaggtgaa aacaccccac
      721 atattatcga tgctactgta gattttatgg catcaagcca ggcatttcgt gagtatctga
      781 aaaaacttcc ccctgataac acaataccgc cagctcttac tgatgagcac gtaccgttat
      841 atatacgaag gctggaatac tatcgtctgc tttatcgacc aaagcagaca gaatcatatt
      901 gaaaaaaacg gtgaacggtt cgcgaaattc tttcgcgggt cgtgaggcgt tggacaagcg
      961 aagcgcgtca gtttttccgg ctcttccccg tgacaggttt ctccaccagt ttatcttcca
     1021 gtgtcacaac tttctgatac accgccgctg tggcgttgta gttcctgtcg agtattgttt
     1081 tcattcccca tccgagcaca cagaacataa ccagaaaagc cactgacacc ccacccagcc
     1141 atttccagct atacccttta agggtgttgt attcctgcac gatattgctg tgaattgtcg
     1201 caatatccct gttcagggca tccgcgtttg ccttcatgtt tgcgactgac tcttccagcc
     1261 gctgaaaatc ctggctgatg cgctcaccta tcagtcctgc cacgtccagc ttctgcacct
     1321 catttctgac ttcatccctg acctgttttg ccacaccgtc agaaatatcc cgatatccgc
     1381 gaagagtttc ccttgccaca gccagagaat cagcaacagc tttctgttgc tccattgcgg
     1441 tatgttgcag atgctccacc gataacagga gcgtaagtaa tttttcttct gtgctggcca
     1501 tgttcagaat cccatagaaa atccacgtga cggtgattta acccgttcag gtgcagttcg
     1561 gttctgtctg ctcactctgt ctctcatgtc aaagccatgc tcccgcgcaa catcatgttg
     1621 cagccttctg accagttcat gttgcttaac cggagtgtcc agccattccc tgatgtcatg
     1681 cccgaccgtt cgcgcctgtt caagatggcg ggaatagtcc ggttcagacc gtttttgcat
     1741 gatgtcatga cgctgggcat catcaagccg tccggcagct tcaaaaccca gatgcacttc
     1801 gggacgtcgt tcaatgccct gttctttcag cgtccggcag tcaatgaagc ccctttcaaa
     1861 gccatgcttt ctcatatgtt cgttgtgctt cacctcccag cgttttcgca attcccgcaa
     1921 ctgctctttt tgctgagtga gagtcaggct tccggaatcc tttttcgcac ctccccgctc
     1981 gggatacctg gcgttatagc gccggaaata ctgttccggt gtacgttcaa tgccatcatt
     2041 cacacgctga ctcatcatga tatgcgcatg aggctgttcg ccgccgtcaa tgctcgcttt
     2101 cggattgtga atggcaaaac tccaggcgtg ccgttcgcca gcttcctgac gtacaaaatc
     2161 ctgcaccagt tcaaggcgct gttccggtgt cagttcacgg ggtaatgcaa tttcaagttc
     2221 cctataggtc gaaccgttcg cacgttcaaa ctggtcagcc gcctgccaga aatgggaagg
     2281 attatccctg gcccattccg gcatgtttcc gtgtgcggaa tattccaaat cctgacggtc
     2341 gcggtatttc tctttgcgct cgatgtaatc ggcgtggttt gcagcctgcc ccttgccacc
     2401 aaatttaacg ctgaggtgat aggttgccac ggtttcagcc ctctgcagga gcgcactgtt
     2461 tctcgacgaa ggagagaaac atataagtgc gcttccttct aattttacga attagcacgg
     2521 aagatacccc ctgcacccgc cgaccgcaag cgatatactg cacgaccaca acacaccgca
     2581 aaaaaacacc agggagcatc acgaacatgc gcatcagcaa catcgaatgg ctgaagaaac
     2641 ggatcggatt catcaggaag ctcggagaac agaccgcacg acagagacag attattgacc
     2701 tgatcgacaa cgaagccgga ctcacagaac aggaacgcaa acttctccac gtactggcaa
     2761 cagcggaaaa aaacgactta caggcacagg agagcgaacg aaaacaggcg gtccaaaaac
     2821 gaatcgaggg aaaaaaacaa cgacgggaac gcaatcaccg gctctttctc gcggctgggc
     2881 tactgatcga agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca
     2941 ggatattaca agcacttaag gaacttaagt acgatctgga gacatcccca aacccagacg
     3001 cgtaagtgcc tccatccgtg ttccgattcc ctggtgcttc ctcgctcatt cgctcgctac
     3061 gctcggtcat gagactgcgg cgagcactac caccacaatc acgcggaaag ccgcgccatt
     3121 gcagggctga acggggaaaa acggcaaaaa acctgctgct caaaaagacg ccagaaaggc
     3181 gctgttatct gatataggct tatttggtct actctacgag acctcgtttt ttcagttggt
     3241 aataccaggc gcgactgatc cctaatgcct cccacggttt cagcgtgcgt gcagatgtag
     3301 ctactgtgct gcgcttagac ttagaaccgc ccttgcgacc gcgtgcagcc tgaatttctg
     3361 gcgtgtgcgt atcagccaca tactgtgcga aagtttccgg cgtgaagttc ctgtgcgtgt
     3421 atttcgcaat gctcttgcca atagcccgac attcagccag tgaaagcgga acgggaagcg
     3481 atgcattgta catttcgaca cgctggatca ccgcttcaag ccactgtgag aacgctggcc
     3541 agccctgacg aatcgccctg taagcccatt tgcgcgtcat ttcgaacagg tggcagtttc
     3601 gccccatccc gtaatgttta tcgatgctac gacgctctga ggcgctcaaa tcgagataat
     3661 cagccagttc atcgagggta taggcttcct cgcgccactc catcaccagc cattccaggt
     3721 ggaacggatt tttgcaaatc aggccgctgt agttcacatc cgcacaaagt ttctcacaca
     3781 gcgaacgttc aaccgctgcg gcatatttca gcgccttaac cgaggcatca ggcgcggttc
     3841 tcacggcgat gttgagtgcg tagagcaggt gagcgtgtcc gttcacagga tttttaacgg
     3901 tgatgttcgg cgcgggagcg ttccggtcgc tccagtcaat cgcagccccc tcgcggtcaa
     3961 cgtcaaaaac cagccagaac tgcgcatgag gctggttctg ctgtatgtac cgcgcaagaa
     4021 gcgcacgccc tttgccggag atgcgaagac caaaagcgag atcatccgtg tgatagggac
     4081 gatggggtaa attttcgtcg aagtattgaa gcgcggcgct catgtgttac gattctctcc
     4141 gggttatgcg acctacacgc cctctgtatt tcatgttgaa tcttgccgga tctggacatg
     4201 atttcagggg cgtttgtttt tcagtcgctt acactacacc caacggcaaa tcctgtcgaa
     4261 ccttcccgaa aacccgatcc atttaccata actcccgttg tacgcccccc ttaaaacgcc
     4321 ctcagtcgct ttctggcgca aaaaacgcaa aaagacaaga aaacaccagg ccaaaccaga
     4381 aaaacgcgtc agaatcgttt tcagggcgtt gcataactat gcacgaaagc gatgtgcgat
     4441 cggtttcgtg cgttctgaac accgaacggc tcaaaacgac aagttttctc ccctggtgcg
     4501 tggcgctctg acggggtttc ctgtttccaa acttcacatc tcgccaggac aaaaaaaaac
     4561 cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt atcggatgct
     4621 caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt tccagaagga
     4681 gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg gaaccaagag
     4741 aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct acgctaaata
     4801 ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga cgaaggattt
     4861 ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca aaaacaagtg
     4921 tagttttcta agtaaattag attcgacaac caattagttt atccacatcg cattccactc
     4981 ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac cagacaccaa
     5041 tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa aactgtcaca
     5101 accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac tgtaacaacc
     5161 tccactctat agttccattc
//
