LOCUS       NZ_CP059293             3066 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain 1506 plasmid p1506-5, complete sequence.
ACCESSION   NZ_CP059293
VERSION     NZ_CP059293.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15548965
            Assembly: GCF_013694285.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Yue,M.
  TITLE     Complete genome sequence of a colistin resistant Escherichia coli
            isolate 1506
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-2020) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:25:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,789
            CDSs (total)                      :: 4,668
            Genes (coding)                    :: 4,435
            CDSs (with protein)               :: 4,435
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 62 of 233
            Pseudo Genes (incomplete)         :: 157 of 233
            Pseudo Genes (internal stop)      :: 44 of 233
            Pseudo Genes (multiple problems)  :: 27 of 233
            Pseudo Genes (short protein)      :: 3 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059293.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="1506"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p1506-5"
                     /geo_loc_name="China: Hangzhou"
                     /collected_by="Meina Yue"
     gene            complement(115..648)
                     /locus_tag="H0481_RS23665"
                     /old_locus_tag="H0481_23665"
     CDS             complement(115..648)
                     /locus_tag="H0481_RS23665"
                     /old_locus_tag="H0481_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168772.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(651..1577)
                     /locus_tag="H0481_RS23670"
                     /old_locus_tag="H0481_23670"
     CDS             complement(651..1577)
                     /locus_tag="H0481_RS23670"
                     /old_locus_tag="H0481_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_014342223.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGYDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1754..2152
                     /locus_tag="H0481_RS23675"
                     /old_locus_tag="H0481_23675"
     CDS             1754..2152
                     /locus_tag="H0481_RS23675"
                     /old_locus_tag="H0481_23675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106645285.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDSILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     Typing          complement(655..1577)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgagcgtt cagaagggcg aacgggtcaa aacagcaaac tttctcatca gaaaacggtg
       61 aacgggacgc ggaatttttt cgcgggtcgt gaggcgttgg acaagcgaag cgcgtcagtt
      121 tttccggctc ttgcccgtga caggtttctc cgccagttta tcttccagtg tcacaacttt
      181 ctgatacacc gccgctgtgg cgttgtagtt cctgtcgagc attgttttca ttccccatcc
      241 aagcacacag aacataacca gaaaagccac tgacacccca cccagccatt tccagctata
      301 ccctttaagg gtgttgtatt cctgcacgat attgctgtga attgtcgcaa tatccctgtt
      361 cagggcatcc gcgtttgcct tcatgtttgc gactgactct tccagccgct gaaaatcctg
      421 gctgatgcgc tcacctatca gccctgcaac atccagcttc tgcacctcgt ttctgacttc
      481 atccctgacc tgttttgcca caccgtcaga aatatcccga tatccgcgaa gtgtttccct
      541 tgccacagcc agagaatcag caacagcttt ctgttgctcc attgcggtat gttgcagatg
      601 ctccaccgat aacaggagcg tgagtaattt ttcttctgtg ctggccatgt tcagaatccc
      661 atagaaaatc cacgtgacgg tgatttaacc cgttcaggtt cagttcggtt ctgtctgctc
      721 actctgtctc tcatgtcaaa gccatgctcc cgcgcaacat catgttgcag ccttctaacc
      781 agttcatgtt gcttaaccgg agtgtccagc cattccctga tgtcataccc gaccgttcgc
      841 gcctgttcaa gatggcggga atagtccggt tcagaccgtt tttgcatgat gtcatgacgc
      901 tgggcatcat caagccgtcc ggcagcttca aaacccagat gcacttcggg acgtcgttca
      961 atgccctgtt ctttcagcgt ccggcagtca atgaagcccc tttcaaagcc atgctttctc
     1021 atatgttcgt tgtgcttcac ctcccagcgt tttcgcaatt cccgcaactg ctctttttgc
     1081 tgagtgagag tcaggcttcc ggaatccttt ttcgcacctc cccgctcggg atacctggcg
     1141 ttatagcgcc ggaaatactg ttccggtgta cgttcaatgc catcattcac acgctggctc
     1201 atcatgatat gcgcatgagg ctgttcgccg ccgtcaatgc tcgctttcgg attgtgaatg
     1261 gcaaaactcc aggcgtgccg ttcgccagct tcctgacgta caaaatcctg caccagttca
     1321 aggcgctgtt ccgctgtcag ttcacggggt aatgcaattt caagttcccg ataggtcgaa
     1381 ccgttcgcac gttcaaactg gtcagccgcc tgccagaaat gtgagggatt atctcgcgcc
     1441 cattccggca tgtttccgtg tgcggaatat tccaaatcct gacggtcgcg gtatttctct
     1501 ttgcgctcga tgtaatcggc gtggtttgca gcctgcccct tgccaccaaa tttaacgctg
     1561 aggtgatagg ttgccacggt ttcagccctc cgcaggagcg cactgtttct cgacgaagga
     1621 gagaaacata taagtgcgct tccttctaat tttacgaatt agcacggaag ataccccctg
     1681 cacccgccga ccgcaagcga tatactgcac gaccacaaca caccgcaaaa aaacaccagg
     1741 gagcaccacg aacatgcgca tcagcaacat cgaatggctg aagaaacgga tcggattcat
     1801 caggaagctc ggagaacaga ccgcacgaca gagacagatt attgacctgc tcgacaacga
     1861 agcaggactc acagaacagg aacgcaaact cctccacgta ctggcaacag cggaaaaaaa
     1921 cgacttacag gcacaggaga gcgaacgaaa acaggcggtc caaaaacgaa tcgagggaaa
     1981 aaaacaacga cgagaacgca atcaccgact ctttctcgcg gctgggctac tgatcgaagc
     2041 aggactggta gacacaaaaa caggcgagct atgctataaa aaagacagta tattacaggc
     2101 acttaaggaa attaagtacg atctggagac atccccaaac ccagacgcgt aagtgcctcc
     2161 atccgtgttc cggttcactt ccgcttcctc gcccactcgc tcactacgtt cggtcgctgg
     2221 ggctgcggcg agcggagtga tctctctctc gatcctgtgg ggatcttttt tttttttgcg
     2281 gtagagcagt ctgtgattgc ggtgcggcag ggggctgtgc catatgctgg ttggtgagcg
     2341 ttctgaggaa ccggcaaact ccccccccac cccgcaaagg gcgtaaaagg gggtaagagt
     2401 tttgcccgcg cgatatgctg gccggagccg cgcccatcgt caccggaacg gacctatggt
     2461 agaacgtccg tttgcatgtt cccgcgtcta cgccgcgaca tttcgcccaa catgctggca
     2521 ctggtcccag aaccgcgccc aagggcaaaa acccaatata agcgcattac attcaaccac
     2581 ttgtgccact ccccaggcgt ccgctcaaaa atgaagtatc ttccccggta gcggaattct
     2641 cacgcgtgcg gtcgccgtcg tcactgaccc cccaaccgca aacggccttt gcaaacacgc
     2701 aacaagtgcc tgcaaaaaca ggttttttcg ggattcctgg gagactgtgc ggcgggaaaa
     2761 ttcttgaaga atacagaagg ttcatgcaaa ctgaatgaac tggtttctgt cgctgcgaac
     2821 aacagactcc acaggaacat tgaggaggtc gaaaacctca atgaacccga agaagctcgc
     2881 ctaatggcgg gcttttttcg ttctactcgc cttcaacgaa acaagcaagt ccaaaaacgc
     2941 aatttgagcg cgatagcaca tcaaatctac aaaaaacgca ctttcacccg accaaacagc
     3001 caaaaacgca ccagaaacgt ttttacgcgc ctgaataact attcacgaaa gcaatgcgcg
     3061 atctgt
//
