LOCUS       NZ_CP059134             1459 bp    DNA     circular CON 31-AUG-2023
DEFINITION  Escherichia coli strain Ec55 plasmid pEc55_4, complete sequence.
ACCESSION   NZ_CP059134
VERSION     NZ_CP059134.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15486685
            Assembly: GCF_019355495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Mahazu,S., Wakano,S., Ayibieke,A., Prah,I., Ablordey,A. and Saito,R.
  TITLE     Whole genome analysis of ESBL producing E.coli ST131 from Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Mahazu,S., Wakano,S., Ayibieke,A., Prah,I., Ablordey,A. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2020) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1000x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/29/2023 04:43:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,347
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,005
            CDSs (with protein)               :: 5,005
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 83 of 228
            Pseudo Genes (incomplete)         :: 166 of 228
            Pseudo Genes (internal stop)      :: 39 of 228
            Pseudo Genes (multiple problems)  :: 52 of 228
            Pseudo Genes (short protein)      :: 3 of 228
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059134.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec55"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc55_4"
                     /geo_loc_name="Ghana"
                     /collection_date="2016"
     gene            1..441
                     /locus_tag="H0X92_RS26450"
                     /old_locus_tag="H0X92_26330"
     CDS             1..441
                     /locus_tag="H0X92_RS26450"
                     /old_locus_tag="H0X92_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          1051..1131
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="87.0"
                     /evalue="4.46e-26"
     Typing          complement(676..936)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagtcggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccggattta aagagccagc ggcaggcgga caatccacag aacattgcat
      481 gcaatttcta gtggattgtc tatcctgcat tgctcttttg ccgcctcaaa atcccttgcg
      541 tgtttttgct ccccgatctc cagaacgacc gagccgccac ggttccggca gcgccttgag
      601 cgcaaccgga aaccgtctgg ggagaccccc agacccccca tcgctgtaac accgacggtc
      661 tacgaccacc taccaaccga gtggctgctt gtaccgtgca aatgggcttt gcagggggct
      721 ggcgcggttc ctggcgacct gaaagcccct ggctaggggt atcggcgcgc ctgacctcct
      781 gaaacgctct gtaagcgttt ttcgcgcctt ccatgccctg acgggcattt aaaccccgcc
      841 tcgtgcgtct gagagcttct gagcgcgttt gaagggctat ctgacgactc atccccgaaa
      901 gaacaacagc ggaatcaccg acagcatgcg agaggtggca ccgctcgccg cagccccagc
      961 gaccgaacgt tagtgagcga gtgggcgagg aagcggaaga gaaccggaaa ccacattgag
     1021 cacgtacgca ctgatacggg gtgtcggggc gaagctctga ccagatggca aacggaatat
     1081 cgtcgcgggt gacggtatta catttgcaca tcctgtcctg atttctgagg cgttttaacg
     1141 gtgaacggac ggcaaaaaca tgatggagct gttgaacgtg gagagcgaga aatcggacaa
     1201 aaatcacggc tgacgaggct tgcagaggag taagccagta tacactccgc tatcgctacg
     1261 tgactgggtc agggcttcgc cccgacaccc caaagagcag ccaggataaa tatctaatgg
     1321 aatcaatgcg ttagacgcct aatataaaga tacagaaagg agaataaaaa cattaataaa
     1381 atctaagggt tacagttgac atagttatca ataatgataa cactgttatc attattgata
     1441 actcaaatag ggttcaaag
//
