LOCUS       NZ_CP059133             2101 bp    DNA     circular CON 31-AUG-2023
DEFINITION  Escherichia coli strain Ec55 plasmid pEc55_3, complete sequence.
ACCESSION   NZ_CP059133
VERSION     NZ_CP059133.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15486685
            Assembly: GCF_019355495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Mahazu,S., Wakano,S., Ayibieke,A., Prah,I., Ablordey,A. and Saito,R.
  TITLE     Whole genome analysis of ESBL producing E.coli ST131 from Ghana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Mahazu,S., Wakano,S., Ayibieke,A., Prah,I., Ablordey,A. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2020) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1000x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/29/2023 04:43:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,347
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,005
            CDSs (with protein)               :: 5,005
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 83 of 228
            Pseudo Genes (incomplete)         :: 166 of 228
            Pseudo Genes (internal stop)      :: 39 of 228
            Pseudo Genes (multiple problems)  :: 52 of 228
            Pseudo Genes (short protein)      :: 3 of 228
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059133.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec55"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc55_3"
                     /geo_loc_name="Ghana"
                     /collection_date="2016"
     gene            complement(660..926)
                     /locus_tag="H0X92_RS26440"
                     /old_locus_tag="H0X92_26320"
     CDS             complement(660..926)
                     /locus_tag="H0X92_RS26440"
                     /old_locus_tag="H0X92_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(930..1895)
                     /locus_tag="H0X92_RS26445"
                     /old_locus_tag="H0X92_26325"
     CDS             complement(930..1895)
                     /locus_tag="H0X92_RS26445"
                     /old_locus_tag="H0X92_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1271..1502)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cctcgtcatt actgggctaa accactattt catatccgcc atttatccct taagtataaa
       61 tacaaggatc acgcgcgaga attgcgcaaa aaataaccac aattttaacc ccccaaaaca
      121 agcacccccg aacggcttac cccaaccttt tccacacacc cgatcaccaa aacagggaat
      181 aaaaacaaaa ccctgtctta caggtggatg gcctcacttc gtgaaaccat ccaccccgca
      241 taagtttttt aatcggtggt gtcgtcaatc actaaatcgg aaccccaaag gggaccccca
      301 cgatttagca tttgacgaga ttaactctcg gagatggcat catgctttta acggacttta
      361 aaaaacggtg aacggatgcg gattttaatc cgtgtctgtg aggcgttgag caacgagcaa
      421 agcgaggcgg tagaaagaga gccagcggca ggctcaggac gataatccgg ggaaaatgta
      481 ccactcgcag agtgttaaca tttgacatcg gattgtctat cctgcattgg tgtcgagttg
      541 gcaaggctga ggataatgct gaggataatg ctgaggataa tgcaggatag acagagcact
      601 agagattgca tgcaatctcg tacctatcac ctcagcctgc cgctggctct tttccagaac
      661 taagaatgcg ttcttaaaaa tccaaacttg ataccgttta aatctatttt cacaaaaaga
      721 gtttttaccc tctcgtttgt tttctgaata tataaaatta attcatcctc aaaaccttcg
      781 tgcggatgaa aaccaaatgt gccaaaactt aatccatcaa taagcaaaat aaggttttta
      841 aacctgtcag gaatcaccgc ttcacttctt aaaaaaaata aaccaatagt cttatttcca
      901 ttcaggtatt caatattaat attcatacgt taatccgatt tatccttctc aggggcgcgt
      961 ttgtaacgcc gtttacctga atcccagaca aacgccgtcc gcttcccgtc atcagtgcca
     1021 tcccctacat cgtcggcaat gacaagatcc tcattggttt ctcgatccaa ctggaggacg
     1081 ttttttagcg ccccaccggt cgagataaaa cggcgcttgt gaagctgccg cgtcagctca
     1141 agaaaccact caggatcgtt tgccatatct tccggtttaa cggagtattt cagcgtttca
     1201 gcaaccgcgc tttgcagttg ctcggcaacg ttggccacaa cctcacctgt cttagttttt
     1261 actgcccgaa tatcgatatt cggctcatag ttcacccgca agcaatcgcg ccagagttct
     1321 acccaacgtt cgtgcttaac gtagttcttc cctttaaacc aagaaggttg caccatcagc
     1381 agacagtgaa aatgcggatg tgcgctgcca tccttacctc gcgtcacctc cgtagccctg
     1441 atccacccct gaacaggtga tagctccttt cgcttttcca ttcgcttaaa cgccgcattc
     1501 attgctgtaa ggactgttcc aagttcacct atctcgcagt tcctgacagt taacgtcaga
     1561 aacaaccatc gggaagacgg gtaatccaca acgattttcg gtagagcctg ataaaaacgg
     1621 gcttgccaca tgagggttct tctccactgg cagacagggc aatgacggac acggcaaaat
     1681 tgcgcactac gaagccgtag gcgcgtttct ccggtttcca tgatggtaga ccaaccaaaa
     1741 cgtaacaaac cgctacagga ggccatacgg agtgcatagc gctcgaattc agcagcagtc
     1801 tggtagatac ccccgacaga atcggtaagc gtgcgctggg tatcccaaac tgcatcacgg
     1861 gggctgtagt ccgaaaggaa tttatcttct gacattaagc ctgctccgat ctgggcaggc
     1921 tgtgatttga tgtggtatat tcatcacgta cctgtgcatt gcgagccagt acatgtcacc
     1981 agagtttgcc gctgaggtga cacattcgaa atgttgggga gtgttgacgc actccccaac
     2041 gccgattaaa aacgcctcaa ttctgctcca ttttctttga cctgtcaaaa cccactcaaa
     2101 g
//
