LOCUS       NZ_CP058966             2458 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ZZW20 plasmid pZZW20-2.4K, complete
            sequence.
ACCESSION   NZ_CP058966
VERSION     NZ_CP058966.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15511588
            Assembly: GCF_013423825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Bai,S.C., Li,R.B., Yang-Yu and Liao,X.P.
  TITLE     Sporadic dissemination of mcr-8-ST11 Klebsiella pneumoniae isolates
            in China
  JOURNAL   Enferm Infecc Microbiol Clin (Engl Ed) (2021) In press
   PUBMED   33632538
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Bai,S.-C. and Liao,X.-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan Street No. 483, Guangzhou, Guangdong
            510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:57:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,778
            CDSs (total)                      :: 5,654
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 43 of 108
            Pseudo Genes (incomplete)         :: 61 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 11 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058966.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZZW20"
                     /isolation_source="sewage"
                     /db_xref="taxon:573"
                     /plasmid="pZZW20-2.4K"
                     /geo_loc_name="China:Fujian"
                     /lat_lon="23.34 N 116.54 E"
                     /collection_date="2018"
                     /collected_by="Shuan-cheng Bai"
     gene            complement(join(2351..2458,1..6))
                     /locus_tag="H0E90_RS28715"
                     /old_locus_tag="H0E90_28715"
     CDS             complement(join(2351..2458,1..6))
                     /locus_tag="H0E90_RS28715"
                     /old_locus_tag="H0E90_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058670733.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     gene            426..617
                     /locus_tag="H0E90_RS28720"
                     /old_locus_tag="H0E90_28720"
     CDS             426..617
                     /locus_tag="H0E90_RS28720"
                     /old_locus_tag="H0E90_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1481..1906)
                     /locus_tag="H0E90_RS28725"
                     /old_locus_tag="H0E90_28725"
     CDS             complement(1481..1906)
                     /locus_tag="H0E90_RS28725"
                     /old_locus_tag="H0E90_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_004884531.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGFIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     Typing          complement(112..551)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.463"
                     /coverage="84.0"
                     /evalue="7.500000000000001e-132"
     Typing          complement(2357..2458)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.146"
                     /coverage="89.0"
                     /evalue="9.59e-38"
ORIGIN
        1 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
       61 gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
      121 agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct gtcgggtttc
      181 gccaccactg atttgagcgt cagattttcg tgatgcttgt caggggggcg gagcctatgg
      241 aaaaacgccc cggcggggcg tggccttttc gcccttgtcc ttagctttcg ttatgcttac
      301 cgttacgtta tctgcttatc ctccctccgc tcgccgcaga ctcatgaccg aacggagtga
      361 gcgaatgagc gaggaagcgg aaaaccatcc gaacgtacag taagcacttt cgcgccgggg
      421 ctttcatgaa caaacaacaa aagactgccg ttaacatggc taaattcatc caggatcagt
      481 ccctgcttct tctggacagg cttaatgagc tggatctgga tcatgaggct gatttctgcg
      541 agaaactcca tgaggatgcg gaacgccttt accgaagcct gtcagcgcgc ctgaatgagc
      601 agcaggacgg tgcgtaatgg ctccgttctg ggtattaccg gagtaatacc ggcatcgcct
      661 taattttcat tgtgttagcg cttaacatta ttaccccggt aatacccaga atccctgtgt
      721 tttttctttt ttatttattt ttcatgaagt tgcgatgaaa atcgcagctg cgttaggtgt
      781 atgtcatttt ttagtatata aaacgaccac tcccgagtgc tggcgccgca ggttaaggat
      841 gattctggct aaaaagcgtc cttttccggg aaagggtcat gctcgtatga ccctatgggt
      901 tcagcgacta cacataaact acaaaattgc atctgctttt accccagaac gcgggtgtac
      961 aacagaatta catcaaaatt acatcacttt tacgtcagat cgcgggtgta caacagaatt
     1021 acatcaaaag tacatcactt ttacgtcact tttacatcat ttttacgtca gatctgacct
     1081 ctgaactatt cgtacatccg ccccctcttc ttgggcctcc tgcacgtgct gaaaatggtt
     1141 tttccggctg tgccggcaac gcctcacggc cacgcaataa attgcgcatc cgttcaccgt
     1201 tatcggggtg gttcgtggtg gctttcaggg cgttcatcgg attttagccg ctaaaatccc
     1261 ggccaggtat gtccgtgcat agccatgcag tcgcgccaga atcatcaggg gcgttttttc
     1321 tcgatatggg cacagaaagg tgtgtgtttc aggccggagg cctcagaaac gccctgaggg
     1381 cctctgaggc ggtgcgggca acggatgtta tggtaaatcc agctgattgg caggtacaaa
     1441 aaaagccccg cagggctttt tttgtaatgc tacgaggaag ctactgctgc ttagaattca
     1501 tttcctcgat atgattgttc acaatcgatc tgaattgcat tggcgccttg atgtttggaa
     1561 ttggagtgtt ggttccgcca gtgcctttaa tcacgataga cccatagtta aagattcgtc
     1621 ccagtatccc ttgatgtatg ccaaggcttt caactttatc aatacggagt tccaccgtat
     1681 tgcgtcggat aaaacccact tttgcgatga ctctttggtt agttaatgca agctccgttg
     1741 accaaactct aaggagcaca gggattatta taagtaaccc taagccgtaa aaaggcagca
     1801 acaagattcc gataattact gggatgattg ttggaatcca ggaggtttga gcgcgagata
     1861 ttacggattc atttctcgtt aatgattggt ctacgtagct tcccataagg ttatatccat
     1921 ttagaaaaaa taggattttt ccctttttaa ggcgaatcag caatagaaac tttatgtctc
     1981 cgtggtgggt atacaaaatt tacggagagc gtagcgaccg tcgcacagtt cggttctttc
     2041 ctttcgtgac gcaactggct tgattctgtg gtggtggtgg ctgctgctat gtctcacata
     2101 ctcttggcgt tgcaatcctt tatcccatat ctgtcgaata tcacagatga tcttcttgag
     2161 atcctgtccg ttgcgtcgta atctctttct ctggaaacga aaaaaccgcc ttgcagggcg
     2221 gtttttcgaa ggttctcaga gctaccaact ctttgaaccg cggtaactgg cttggaggag
     2281 cgcagtaacc aaatctgtcc tttcagttta gccttaaccg gcgcataact tcaagactaa
     2341 ctcctctaaa tcagttacca gtggctgctg ccagtggcgc tttgtcgtgt ctttccgggt
     2401 tggactcaag atgatagtta ccggacaagg cgcagcagtc ggactgaacg gggggttc
//
