LOCUS       NZ_CP058965             3508 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ZZW20 plasmid pZZW20-3.5K, complete
            sequence.
ACCESSION   NZ_CP058965
VERSION     NZ_CP058965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15511588
            Assembly: GCF_013423825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Bai,S.C., Li,R.B., Yang-Yu and Liao,X.P.
  TITLE     Sporadic dissemination of mcr-8-ST11 Klebsiella pneumoniae isolates
            in China
  JOURNAL   Enferm Infecc Microbiol Clin (Engl Ed) (2021) In press
   PUBMED   33632538
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Bai,S.-C. and Liao,X.-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2020) College of Veterinary Medicine, South China
            Agricultural University, Wushan Street No. 483, Guangzhou, Guangdong
            510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:57:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,778
            CDSs (total)                      :: 5,654
            Genes (coding)                    :: 5,546
            CDSs (with protein)               :: 5,546
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 43 of 108
            Pseudo Genes (incomplete)         :: 61 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 11 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058965.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZZW20"
                     /isolation_source="sewage"
                     /db_xref="taxon:573"
                     /plasmid="pZZW20-3.5K"
                     /geo_loc_name="China:Fujian"
                     /lat_lon="23.34 N 116.54 E"
                     /collection_date="2018"
                     /collected_by="Shuan-cheng Bai"
     gene            380..571
                     /locus_tag="H0E90_RS28700"
                     /old_locus_tag="H0E90_28700"
     CDS             380..571
                     /locus_tag="H0E90_RS28700"
                     /old_locus_tag="H0E90_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            571..993
                     /locus_tag="H0E90_RS28705"
                     /old_locus_tag="H0E90_28705"
     CDS             571..993
                     /locus_tag="H0E90_RS28705"
                     /old_locus_tag="H0E90_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_065808023.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGVNSVGLHDLQHRITLLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1937..3073)
                     /locus_tag="H0E90_RS28710"
                     /old_locus_tag="H0E90_28710"
     CDS             complement(1937..3073)
                     /locus_tag="H0E90_RS28710"
                     /old_locus_tag="H0E90_28710"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065808024.1"
                     /translation="MEGIEKYIIEEYKSKKDTYIRLGQSMNSLVDTLLHTSGIKAYTVQ
                     HRVKDEGSLKDKIQLKNKYKTISDITDILGLRIITYYSSDTDLVEDIIKNEFAVDSENT
                     IDKRKKDDPDRFGYMSLHYVVSLDEKRSNLKENIKFKGIKFEIQIRTILQHTWAEIEHD
                     LGYKSKSSVPLHIRRKFSSLSGALELIDGAFIEIRKSLAAYDEETKNQITAIESTSKDT
                     QKRKNNKANHEVSDINAILIKNFIISSPMMQDLYDAYINQEKSKGVIERVIYPLDAKSD
                     LDVNYENLALILKKLNAKDIGDILEITSEISEDPYIITTIVNNRPDYMPAGVLSKYFLF
                     LFCIYIYIHKKELHDSFKETGSINLISQLGAAYSQIKK"
                     /name="hypothetical protein"
     gene            complement(3359..3472)
                     /locus_tag="H0E90_RS29145"
     CDS             complement(3359..3472)
                     /locus_tag="H0E90_RS29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074422211.1"
                     /translation="MHEPPVQSDCCASSGNYRLESNPVKHAKAPLAAAIGN"
                     /name="replication initiation protein"
     Typing          complement(2..332)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.799"
                     /coverage="92.0"
                     /evalue="6.579999999999999e-92"
     Typing          complement(3360..3508)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.333"
                     /coverage="99.0"
                     /evalue="2.49e-60"
ORIGIN
        1 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
       61 gaatggcagg aacaggagag cgcacgaggg agccatcagg gggaaacgcc tggtatcttt
      121 atagtcctgt cgggtttcgc caccactgat ttgagcgtca aattctgtga tgcttgtcag
      181 gggggcggag cctatggaaa aacggccgct aagcggcctt tccttttccc cgtcacgcga
      241 ccgctcggtt ttctcgagct tttacacgca tgctcctgcc gctcgccgca gccgaacggc
      301 aggagcatag cgactgagtg agcgaggaag cggaaaagag aacatcccta cactgagcac
      361 tgacgctctg aggcctctta tgacgaaaca acagaaaaca gcgctgaaca tggctaaatt
      421 catccagaat cagtctctgc tcctgctgga gaagcttaac gagcttgatc ttgatgtaga
      481 ggccgacctt tgtgagaaac tccacgatga tgcggagcat cttttccgta cactgtcctc
      541 gaggctggat gaattacagg atggtaactg atggctagag tgtctattag tgaagcggca
      601 cggcttgtta aggttagtcg tcctacaatt tacaagatga ttaacagtgg aaagttaagt
      661 tacacctctg ttgtaaagca cgggaaggct attaaagtta ttgatacttc tgagcttatt
      721 agagtgtttg gttctcttga tggtgtaatc gatgctgtaa aatatgatgt aaaaagtgac
      781 gcagaatcta caggtgttaa cagcgttggt ttacatgatt tacaacatag aatcacttta
      841 cttgaagctg aaaacgatgg tcttaaaggg gctgttaagg ctcgagatga gcatattgat
      901 tcccttcgtc aggctatgca gcttctggag cataagcatg aaccttcttc accaccccat
      961 tccccgtggt ggaaattctg gaaaaaatcc tgaccccgtc atgcttcagt tttcctctcc
     1021 ggccatcccg gggagttcct gaaccttccc aaaaatcgcc agatctctga acgctcctca
     1081 ccactaaaaa tacccacacc ccacgcaaaa ttaaaatttt gaagataact cattaatatc
     1141 agttagttgt gggttattaa attacgcagc attactttct gtaaggtgaa tccaagtcgt
     1201 cggcgattcg tgtacgactt gccgacgact cgtcatagag tcatcgacga ctcacttaag
     1261 actatcagta acttatgacg acttgactct agagtcgaat cgtcattttg gtgttttttt
     1321 gaccttgacg actctagagt caagtcacga gtcgaatcgc cggtgaatcg tgtggtgagt
     1381 cgtccggcga cttgccataa cgtgattgcc ggccgtcgac gaacccgatt cctgatcgtg
     1441 cgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc
     1501 ccgttcaccg ttttcggata ggaagaacgc cggaaacagg acttaccctg gctgaatggc
     1561 gtgaattttc gcgctatgca cttgcgtgca tactcatgca tgccgtaaaa acagagcctg
     1621 cgcgtttctg gcgggttttc gggtggtttg ttgcttgttt taccggtttc ccgtcagaaa
     1681 cgccctgagg ccgttttagc ggtgcgtaca atcatggctt tatgttaaat tgattaggcg
     1741 ggggttaagt atttttccat gaccctatct aatcaacgct tattcggtag gtttttttat
     1801 ttagggaagc gcaatgcttt acaaacgaga tgctatgcat aaacttttat gttattactt
     1861 gtttgttctc ttttttattt tatttacagc cccgccaaca taaatcattt aaagctggcg
     1921 ggagaagtta tggtttttat ttcttaattt gtgagtaggc cgcccctagt tgagatatta
     1981 aattaataga tcctgtttct ttaaatgaat catgtaattc ttttttgtga atataaatat
     2041 atatacaaaa taggaataga aaatattttg ataaaacccc agcaggcata taatccggtc
     2101 tgttattgac aatcgttgtt attatatacg gatcctcgct gatctcagag gtgatttcta
     2161 atatatcacc aatgtcttta gcatttaact ttttaagaat tagtgcgagg ttttcgtaat
     2221 taacatcaag atcactcttc gcatcaagtg gataaataac tctctcgata acgccttttg
     2281 atttttcttg attgatgtat gcatcataaa gatcctgcat cattggtgat gaaatgatga
     2341 agtttttaat taaaattgca tttatgtcgc taacttcatg atttgctttg ttgttttttc
     2401 ttttttgtgt gtcttttgat gtggattcaa ttgccgttat ttgatttttt gtctcttcgt
     2461 cataagcggc taaagacttt cttatttcaa taaacgcgcc atctataagc tctagagccc
     2521 cggaaaggct agagaatttc cttcttatat gtaatggcac tgagcttttt gatttgtagc
     2581 caaggtcatg ttctatttct gcccatgtgt gttgtagtat tgttctgatt tgtatttcga
     2641 atttaatccc tttgaatttg atattttctt tgagattaga tctcttttca tccaaggaaa
     2701 ctacgtaatg tagtgacata taaccgaatc tatccggatc atctttcttt cttttatcaa
     2761 tcgtattttc gctatcaaca gcaaattcgt ttttgattat atcttctacc aggtcggtat
     2821 cacttgagta gtaagttatt attcttaatc caagaatgtc cgttatgtca gatatcgttt
     2881 tgtacttatt ttttaattgt atcttgtctt taaggctccc ttcatccttt actctgtgct
     2941 gaacggtgta tgcctttatc cctgaagtat gcagtaatgt atccaccaat gaattcattg
     3001 attgaccaag cctaatataa gtgtctttct tagacttata ttcttctatt atatattttt
     3061 cgatgccttc cataattatc tcattgataa acttttttaa attaggtttt tattttttcc
     3121 atgattatgt tttatagcat ttttcttttg atgggaacta tttcttgaat tttttttatt
     3181 gcagacgtaa tattctgttt gaaaacaaaa aaccacccgg caaggtggtt ttttgaaggt
     3241 tcaaaaactt gcaggtgatt gaaccaaggt aactggctag tagaggagct cagtcaccaa
     3301 aactgttcgt ttagtttagc ctcaattggc tcataacttc aagactaact cctctaaatc
     3361 agttaccaat ggctgctgcc agtggcgctt tcgcatgctt taccgggttg gactcaagac
     3421 gatagttacc ggatgaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca
     3481 gcttggagcg aactgcctac ccggaact
//
